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The map label for this gene is alaXM [H]

Identifier: 186476790

GI number: 186476790

Start: 2286591

End: 2287322

Strand: Reverse

Name: alaXM [H]

Synonym: Bphy_2035

Alternate gene names: 186476790

Gene position: 2287322-2286591 (Counterclockwise)

Preceding gene: 186476791

Following gene: 186476789

Centisome position: 65.74

GC content: 68.31

Gene sequence:

>732_bases
ATGACGACGCGCGCGCTCTTTCGCGACGACGCCTATCTGACCCGCTGCGACGCGACCATCACCGCGATCGACGAGCAGGG
CATCCATCTCGATCAGACCGTGTTCTATCCGCTCGGCGGCGGCCAGGCGGGCGACGCCGGTGTGCTGACGCTCGCGGACG
GCACGCGAATCCAGATCGCCGACACCCGCAAGGCGAAGTTCGAAGGCGCGACGCCCGACGACGCCGTCCATGTTCCCGCG
CCCGGGCAGGAAGCGCTGCTGGCGCAACTCGCGGCCGGCCAGCCGTTGACGGCGGAGATCGACTGGGGCCGCCGCTACCG
GCATATGCGCCTGCACACGGCGAGTCATCTGATGTGTGCGGTGCTGCCGTATCCCGTCGACGGCTGCAGCATCACGTCCG
ACTATGCGCGTCTCGACTTCGCGACGGTCGAGCCGATCGGACGCGAGGAAGTCGAAACGCGGCTCGCGGAACTGGTGGGC
GGTGCGCACGGTGTCGCGACACAATGGATCACGGACGACGAAATGGCGTCGCGCCCCGAACTCGTGCGCACGATGAGCGT
GAAGCCGCCGATGGGCCTCGGCCGAGTGCGCCTGTTGCGCATCGAAGGCGTCGACCTGCAGCCGTGCGGCGGCACGCACG
TGCGCAACACGCAGGAGATCGGCGGGCTGCGCGTCGCAAAACTCGAAAAGAAAAGCGCGCGCACGCGGCGGCTCGTGCTG
GAGCTTGCATGA

Upstream 100 bases:

>100_bases
GCGAGGCGAGCGGCGCGCGCATCGGCATAATGTTCGCTGGCACACCCCGGGCATGAGCCAGGCGAATGCCGGACGCAAAC
CAGGCACAAGGAGACTTTCC

Downstream 100 bases:

>100_bases
GCGTGCGTATCGACGCGGCTGCATATTCGTCGGAGGCGGACTACGCCGCGCTCGATCCTCGCGCGCGCGATGTGCTCGAC
TGCTGGTTCGGCTTGCCCGG

Product: threonyl/alanyl tRNA synthetase SAD

Products: AMP; diphosphate; L-alanyl-tRNAAla [C]

Alternate protein names: AlaX-M; AlaXp; Alanyl-tRNA deacylase AlaX-M [H]

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MTTRALFRDDAYLTRCDATITAIDEQGIHLDQTVFYPLGGGQAGDAGVLTLADGTRIQIADTRKAKFEGATPDDAVHVPA
PGQEALLAQLAAGQPLTAEIDWGRRYRHMRLHTASHLMCAVLPYPVDGCSITSDYARLDFATVEPIGREEVETRLAELVG
GAHGVATQWITDDEMASRPELVRTMSVKPPMGLGRVRLLRIEGVDLQPCGGTHVRNTQEIGGLRVAKLEKKSARTRRLVL
ELA

Sequences:

>Translated_243_residues
MTTRALFRDDAYLTRCDATITAIDEQGIHLDQTVFYPLGGGQAGDAGVLTLADGTRIQIADTRKAKFEGATPDDAVHVPA
PGQEALLAQLAAGQPLTAEIDWGRRYRHMRLHTASHLMCAVLPYPVDGCSITSDYARLDFATVEPIGREEVETRLAELVG
GAHGVATQWITDDEMASRPELVRTMSVKPPMGLGRVRLLRIEGVDLQPCGGTHVRNTQEIGGLRVAKLEKKSARTRRLVL
ELA
>Mature_242_residues
TTRALFRDDAYLTRCDATITAIDEQGIHLDQTVFYPLGGGQAGDAGVLTLADGTRIQIADTRKAKFEGATPDDAVHVPAP
GQEALLAQLAAGQPLTAEIDWGRRYRHMRLHTASHLMCAVLPYPVDGCSITSDYARLDFATVEPIGREEVETRLAELVGG
AHGVATQWITDDEMASRPELVRTMSVKPPMGLGRVRLLRIEGVDLQPCGGTHVRNTQEIGGLRVAKLEKKSARTRRLVLE
LA

Specific function: Functions in trans to edit the amino acid moiety from mischarged Ser-tRNA(Ala). Recognition depends, at least in part, on the acceptor stem of tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-M subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789048, Length=233, Percent_Identity=29.6137339055794, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7 [C]

Molecular weight: Translated: 26435; Mature: 26303

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRALFRDDAYLTRCDATITAIDEQGIHLDQTVFYPLGGGQAGDAGVLTLADGTRIQIA
CCCCCEECCCCEEEECCEEEEEECCCCCEECCEEEEECCCCCCCCCEEEEECCCCEEEEE
DTRKAKFEGATPDDAVHVPAPGQEALLAQLAAGQPLTAEIDWGRRYRHMRLHTASHLMCA
CCCCCEECCCCCCCEEECCCCCHHHHHHHHHCCCCEEEEECCCCCEEEEEEHHHHHEEEE
VLPYPVDGCSITSDYARLDFATVEPIGREEVETRLAELVGGAHGVATQWITDDEMASRPE
ECCCCCCCCEECCCHHHEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEECCHHHHCCCH
LVRTMSVKPPMGLGRVRLLRIEGVDLQPCGGTHVRNTQEIGGLRVAKLEKKSARTRRLVL
HHHEECCCCCCCCCCEEEEEEECCEECCCCCCCCCCHHHHCCEEEEEHHHHHHHHEEEEE
ELA
EEC
>Mature Secondary Structure 
TTRALFRDDAYLTRCDATITAIDEQGIHLDQTVFYPLGGGQAGDAGVLTLADGTRIQIA
CCCCEECCCCEEEECCEEEEEECCCCCEECCEEEEECCCCCCCCCEEEEECCCCEEEEE
DTRKAKFEGATPDDAVHVPAPGQEALLAQLAAGQPLTAEIDWGRRYRHMRLHTASHLMCA
CCCCCEECCCCCCCEEECCCCCHHHHHHHHHCCCCEEEEECCCCCEEEEEEHHHHHEEEE
VLPYPVDGCSITSDYARLDFATVEPIGREEVETRLAELVGGAHGVATQWITDDEMASRPE
ECCCCCCCCEECCCHHHEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEECCHHHHCCCH
LVRTMSVKPPMGLGRVRLLRIEGVDLQPCGGTHVRNTQEIGGLRVAKLEKKSARTRRLVL
HHHEECCCCCCCCCCEEEEEEECCEECCCCCCCCCCHHHHCCEEEEEHHHHHHHHEEEEE
ELA
EEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; @ALAN01.txt*L-alanine!; tRNAAla [C]

Specific reaction: ATP + @ALAN01.txt*L-alanine! + tRNAAla = AMP + diphosphate + L-alanyl-tRNAAla [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824 [H]