Click here to switch to the map view.

The map label for this gene is prpE [H]

Identifier: 186476346

GI number: 186476346

Start: 1799620

End: 1801620

Strand: Direct

Name: prpE [H]

Synonym: Bphy_1588

Alternate gene names: 186476346

Gene position: 1799620-1801620 (Clockwise)

Preceding gene: 186476345

Following gene: 186476349

Centisome position: 51.73

GC content: 64.12

Gene sequence:

>2001_bases
ATGCTGTCCTCTCGCGTAAGCGAAAAAAGAACCACAGCAAAACCGCAGGAACTGCAAAAAGCCGCACCCGTGAAAGGACA
TCCTCCGATGACCACCTACCGCGAGTTCCACCGCCGGTCGATCGAATCCCCGGAAGACTTCTGGCGCGAACAGGCCAACC
GGATCCATTGGGAAACCAGATTCGAAACCGTTCTCGACCGCTCGAATCCGCCATTTTCACGCTGGTTCGTCGGCGGCAGG
ACGAATCTCTGCCATAACGCCGTCGACCGGCACCTCGCGGAACGCGCGCAGCAGAACGCGCTCATCTATGTGTCGACGGA
AACGGGCATCGAGCGGCAATATACCTACGCCGAACTTCACGCGGAAGTGAACCGGATGGCAGCCGTGATGCGCTCGCTCG
GCGTCAAGCGCAGCGACCGCGTGCTGATCTACCTGCCCATGATCCCCGAAGCCGTGTTCGCGATGCTCGCGTGCGCGCGG
CTCGGCGCAATTCACTCAGTCGTGTTCGGCGGCTTTGCCGCGCCGAACCTTGCCGCGCGCATCGACGACGCGAAGCCCGT
GCTGATCGTCACCGCCGACGCGGGGGCGCGCGCAGGCAAAGTAATCGACTACACACCGCTCGTCGATGAAGCGATGTCGC
GCGCGACGCACAAGACGCCGCACGTGCTGCTGATCGACCGGCAACTGACGCCCGAGCGTCTGAACGCGCCGTATCTCGTC
GCGTACGAGCCACTGCGCGAGCAGTTCTTCGATGTCCACGTGCAATGCGAATGGCTGGAATCGACGGAACCTTCGTATGT
GCTCTATACGTCGGGCACGACGGGCAAGCCGAAAGGCGTGCAGCGCGACGTCGGCGGCTATGCCGTGGCGCTCGCGGCGT
CGATGGAATACATCTTCCAGGGCAAGCCGGCCGACACGATGTTCACCGCCTCCGACGTCGGCTGGGTCGTCGGTCACAGC
TACATCGTCTACGCGCCGCTGATCGCCGGTCTCACGACAGTCATGTACGAGGGCACGCCGATCCGTCCCGACGGCGGTAT
CTGGTGGCGGCTCGTCGAACAGTACAAGATCAATCTGATGTTTACCGCGCCGACAGCGATCCGCGTGCTAAAGAAGCAGG
ATCCCGCGCTGCTCAAACAGGCCGACTTGTCCAGCCTGCGTACGCTGTTTCTCGCCGGCGAGCCGCTCGACGAACCGACA
GCTTCATGGATCGCCGACTCGCTCGGCAAACCCGTCGTCGACAACTTCTGGCAGACGGAAACCGGCTGGCCGATTCTCGC
AATCCAGCGCGGCGTCGAGGCGTTGCCGCAAAAACTCGGCTCTCCGGGCGTGCCCTGCTACGGCTACGATCTGACGCTGC
GCAACGAGAACACGGGCGAGCCGTGCCCGCCGGGCGAAAAAGGCGTGATCACGCTCGGCTATCCGTTGCCGCCGGGCTGT
ATGTCGACCGTGTGGGGCGATGATGCGCGCTTCGTGAAGACCTATTGGGAAAGCGTGCCGAACCAGCAGGTCTATTCGAC
TTTCGACTGGGGTATCCAGGACGAAGACGGCTACGTGACGATCCTCGGCCGCACCGACGATGTGATCAACGTCGCGGGCC
ACCGGCTCGGCACGCGCGAAATCGAGGAAGCGCTGTCGAGCCACAAGGCGGTGGCGGAAGTCGCCGTGGTCGGCGTGACG
GATCAGGTCAAAGGACAGGCGGCGATTGCGTTCGTCGTCGTGCGCGATGCGGCTGCATATGATTCGGACGACGCGCGCGC
GAAGCTCTCTGCCGACCTGTGCGCGACCGTCGATCGCCAGCTCGGCGCGATCGCGCGGCCCGCGCAGGTGGCGCTGGTGT
CGATGCTGCCCAAGACGCGCTCCGGCAAGCTGTTGCGCCGCGCGATCGCGGCGCTTGCCGAAGGACGCGACCCCGGCGAC
CTGCCGACCATCGAAGATCCATCCGCTTTGCAGCAGATTCGTGACGCCGTGGCACTCGGTTTCGGCACACGCGGCGGCTG
A

Upstream 100 bases:

>100_bases
CAGCGAAAGAAACGCGCTCGCCAGCAAAAAACCAGAACGGCGCTGCAGACAAAAAAAGGTCATGGTGAACCCGCGTTGCC
CGCCCCACAGGCGGTGCTCT

Downstream 100 bases:

>100_bases
GCGTCGCCGCCAACGCGGCGCGGGGTACTTAATTTCGGCTCATCCGCGCGCCGACAAAAACCGCTTCAGTATTCCACTCG
AATACGTGCCCGCGATCTCG

Product: propionyl-CoA synthetase

Products: NA

Alternate protein names: Propionyl-CoA synthetase [H]

Number of amino acids: Translated: 666; Mature: 666

Protein sequence:

>666_residues
MLSSRVSEKRTTAKPQELQKAAPVKGHPPMTTYREFHRRSIESPEDFWREQANRIHWETRFETVLDRSNPPFSRWFVGGR
TNLCHNAVDRHLAERAQQNALIYVSTETGIERQYTYAELHAEVNRMAAVMRSLGVKRSDRVLIYLPMIPEAVFAMLACAR
LGAIHSVVFGGFAAPNLAARIDDAKPVLIVTADAGARAGKVIDYTPLVDEAMSRATHKTPHVLLIDRQLTPERLNAPYLV
AYEPLREQFFDVHVQCEWLESTEPSYVLYTSGTTGKPKGVQRDVGGYAVALAASMEYIFQGKPADTMFTASDVGWVVGHS
YIVYAPLIAGLTTVMYEGTPIRPDGGIWWRLVEQYKINLMFTAPTAIRVLKKQDPALLKQADLSSLRTLFLAGEPLDEPT
ASWIADSLGKPVVDNFWQTETGWPILAIQRGVEALPQKLGSPGVPCYGYDLTLRNENTGEPCPPGEKGVITLGYPLPPGC
MSTVWGDDARFVKTYWESVPNQQVYSTFDWGIQDEDGYVTILGRTDDVINVAGHRLGTREIEEALSSHKAVAEVAVVGVT
DQVKGQAAIAFVVVRDAAAYDSDDARAKLSADLCATVDRQLGAIARPAQVALVSMLPKTRSGKLLRRAIAALAEGRDPGD
LPTIEDPSALQQIRDAVALGFGTRGG

Sequences:

>Translated_666_residues
MLSSRVSEKRTTAKPQELQKAAPVKGHPPMTTYREFHRRSIESPEDFWREQANRIHWETRFETVLDRSNPPFSRWFVGGR
TNLCHNAVDRHLAERAQQNALIYVSTETGIERQYTYAELHAEVNRMAAVMRSLGVKRSDRVLIYLPMIPEAVFAMLACAR
LGAIHSVVFGGFAAPNLAARIDDAKPVLIVTADAGARAGKVIDYTPLVDEAMSRATHKTPHVLLIDRQLTPERLNAPYLV
AYEPLREQFFDVHVQCEWLESTEPSYVLYTSGTTGKPKGVQRDVGGYAVALAASMEYIFQGKPADTMFTASDVGWVVGHS
YIVYAPLIAGLTTVMYEGTPIRPDGGIWWRLVEQYKINLMFTAPTAIRVLKKQDPALLKQADLSSLRTLFLAGEPLDEPT
ASWIADSLGKPVVDNFWQTETGWPILAIQRGVEALPQKLGSPGVPCYGYDLTLRNENTGEPCPPGEKGVITLGYPLPPGC
MSTVWGDDARFVKTYWESVPNQQVYSTFDWGIQDEDGYVTILGRTDDVINVAGHRLGTREIEEALSSHKAVAEVAVVGVT
DQVKGQAAIAFVVVRDAAAYDSDDARAKLSADLCATVDRQLGAIARPAQVALVSMLPKTRSGKLLRRAIAALAEGRDPGD
LPTIEDPSALQQIRDAVALGFGTRGG
>Mature_666_residues
MLSSRVSEKRTTAKPQELQKAAPVKGHPPMTTYREFHRRSIESPEDFWREQANRIHWETRFETVLDRSNPPFSRWFVGGR
TNLCHNAVDRHLAERAQQNALIYVSTETGIERQYTYAELHAEVNRMAAVMRSLGVKRSDRVLIYLPMIPEAVFAMLACAR
LGAIHSVVFGGFAAPNLAARIDDAKPVLIVTADAGARAGKVIDYTPLVDEAMSRATHKTPHVLLIDRQLTPERLNAPYLV
AYEPLREQFFDVHVQCEWLESTEPSYVLYTSGTTGKPKGVQRDVGGYAVALAASMEYIFQGKPADTMFTASDVGWVVGHS
YIVYAPLIAGLTTVMYEGTPIRPDGGIWWRLVEQYKINLMFTAPTAIRVLKKQDPALLKQADLSSLRTLFLAGEPLDEPT
ASWIADSLGKPVVDNFWQTETGWPILAIQRGVEALPQKLGSPGVPCYGYDLTLRNENTGEPCPPGEKGVITLGYPLPPGC
MSTVWGDDARFVKTYWESVPNQQVYSTFDWGIQDEDGYVTILGRTDDVINVAGHRLGTREIEEALSSHKAVAEVAVVGVT
DQVKGQAAIAFVVVRDAAAYDSDDARAKLSADLCATVDRQLGAIARPAQVALVSMLPKTRSGKLLRRAIAALAEGRDPGD
LPTIEDPSALQQIRDAVALGFGTRGG

Specific function: Catalyzes the synthesis of propionyl-CoA from propionate and CoA. Also converts acetate to acetyl-CoA but with a lower specific activity [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI13375727, Length=639, Percent_Identity=40.3755868544601, Blast_Score=468, Evalue=1e-131,
Organism=Homo sapiens, GI8923896, Length=681, Percent_Identity=36.7107195301028, Blast_Score=418, Evalue=1e-117,
Organism=Homo sapiens, GI253970489, Length=694, Percent_Identity=36.3112391930836, Blast_Score=413, Evalue=1e-115,
Organism=Homo sapiens, GI28416953, Length=637, Percent_Identity=38.3045525902669, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI122937307, Length=572, Percent_Identity=29.5454545454545, Blast_Score=189, Evalue=8e-48,
Organism=Homo sapiens, GI38505220, Length=563, Percent_Identity=28.0639431616341, Blast_Score=178, Evalue=1e-44,
Organism=Homo sapiens, GI42544132, Length=544, Percent_Identity=27.0220588235294, Blast_Score=175, Evalue=1e-43,
Organism=Homo sapiens, GI31982927, Length=651, Percent_Identity=24.1167434715822, Blast_Score=166, Evalue=6e-41,
Organism=Homo sapiens, GI58082049, Length=553, Percent_Identity=27.6672694394213, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI115511026, Length=551, Percent_Identity=26.8602540834846, Blast_Score=153, Evalue=5e-37,
Organism=Homo sapiens, GI157311624, Length=553, Percent_Identity=27.124773960217, Blast_Score=150, Evalue=6e-36,
Organism=Homo sapiens, GI157311622, Length=553, Percent_Identity=27.124773960217, Blast_Score=150, Evalue=6e-36,
Organism=Homo sapiens, GI42544134, Length=365, Percent_Identity=23.5616438356164, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI195947378, Length=451, Percent_Identity=24.6119733924612, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI187761345, Length=394, Percent_Identity=24.1116751269036, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI187761343, Length=394, Percent_Identity=24.1116751269036, Blast_Score=73, Evalue=7e-13,
Organism=Escherichia coli, GI1786529, Length=626, Percent_Identity=61.6613418530351, Blast_Score=786, Evalue=0.0,
Organism=Escherichia coli, GI1790505, Length=641, Percent_Identity=38.3775351014041, Blast_Score=426, Evalue=1e-120,
Organism=Escherichia coli, GI221142682, Length=558, Percent_Identity=22.0430107526882, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1788107, Length=553, Percent_Identity=23.1464737793852, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1786810, Length=472, Percent_Identity=23.9406779661017, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1786801, Length=495, Percent_Identity=23.030303030303, Blast_Score=83, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71982997, Length=648, Percent_Identity=36.1111111111111, Blast_Score=378, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI71983001, Length=648, Percent_Identity=36.1111111111111, Blast_Score=377, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17569507, Length=649, Percent_Identity=22.0338983050847, Blast_Score=129, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17560308, Length=566, Percent_Identity=24.2049469964664, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71985884, Length=596, Percent_Identity=23.8255033557047, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17531443, Length=381, Percent_Identity=24.1469816272966, Blast_Score=88, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71996755, Length=525, Percent_Identity=21.7142857142857, Blast_Score=82, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17560140, Length=533, Percent_Identity=21.5759849906191, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319264, Length=647, Percent_Identity=36.9397217928903, Blast_Score=435, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6323182, Length=666, Percent_Identity=37.5375375375375, Blast_Score=426, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6319699, Length=383, Percent_Identity=22.976501305483, Blast_Score=87, Evalue=7e-18,
Organism=Drosophila melanogaster, GI21355225, Length=659, Percent_Identity=40.8194233687405, Blast_Score=477, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24667955, Length=650, Percent_Identity=36.1538461538462, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI62472339, Length=562, Percent_Identity=37.5444839857651, Blast_Score=350, Evalue=3e-96,
Organism=Drosophila melanogaster, GI24667959, Length=529, Percent_Identity=37.0510396975425, Blast_Score=320, Evalue=2e-87,
Organism=Drosophila melanogaster, GI21355181, Length=538, Percent_Identity=23.4200743494424, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21358303, Length=506, Percent_Identity=21.1462450592885, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI18859661, Length=568, Percent_Identity=21.830985915493, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR012694 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.17 [H]

Molecular weight: Translated: 73245; Mature: 73245

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSSRVSEKRTTAKPQELQKAAPVKGHPPMTTYREFHRRSIESPEDFWREQANRIHWETR
CCCCHHHHHHCCCCHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCEEHHHH
FETVLDRSNPPFSRWFVGGRTNLCHNAVDRHLAERAQQNALIYVSTETGIERQYTYAELH
HHHHHCCCCCCHHHEEECCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHH
AEVNRMAAVMRSLGVKRSDRVLIYLPMIPEAVFAMLACARLGAIHSVVFGGFAAPNLAAR
HHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEE
IDDAKPVLIVTADAGARAGKVIDYTPLVDEAMSRATHKTPHVLLIDRQLTPERLNAPYLV
CCCCCEEEEEEECCCCCCCCEEECCHHHHHHHHHHHCCCCEEEEEECCCCHHHCCCCEEE
AYEPLREQFFDVHVQCEWLESTEPSYVLYTSGTTGKPKGVQRDVGGYAVALAASMEYIFQ
EEHHHHHHHEEEEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCEEEHHHHHHHHHCC
GKPADTMFTASDVGWVVGHSYIVYAPLIAGLTTVMYEGTPIRPDGGIWWRLVEQYKINLM
CCCCCCEEECCCCCHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHEEEEEEEEEE
FTAPTAIRVLKKQDPALLKQADLSSLRTLFLAGEPLDEPTASWIADSLGKPVVDNFWQTE
EECCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHCCCCC
TGWPILAIQRGVEALPQKLGSPGVPCYGYDLTLRNENTGEPCPPGEKGVITLGYPLPPGC
CCCEEHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCH
MSTVWGDDARFVKTYWESVPNQQVYSTFDWGIQDEDGYVTILGRTDDVINVAGHRLGTRE
HHHHCCCCHHHHHHHHHHCCCCHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHCCCCHHH
IEEALSSHKAVAEVAVVGVTDQVKGQAAIAFVVVRDAAAYDSDDARAKLSADLCATVDRQ
HHHHHHHHHHHHHEEEEECCHHCCCCEEEEEEEEECHHCCCCCCCHHHHHHHHHHHHHHH
LGAIARPAQVALVSMLPKTRSGKLLRRAIAALAEGRDPGDLPTIEDPSALQQIRDAVALG
HHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHC
FGTRGG
CCCCCC
>Mature Secondary Structure
MLSSRVSEKRTTAKPQELQKAAPVKGHPPMTTYREFHRRSIESPEDFWREQANRIHWETR
CCCCHHHHHHCCCCHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCEEHHHH
FETVLDRSNPPFSRWFVGGRTNLCHNAVDRHLAERAQQNALIYVSTETGIERQYTYAELH
HHHHHCCCCCCHHHEEECCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHH
AEVNRMAAVMRSLGVKRSDRVLIYLPMIPEAVFAMLACARLGAIHSVVFGGFAAPNLAAR
HHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEE
IDDAKPVLIVTADAGARAGKVIDYTPLVDEAMSRATHKTPHVLLIDRQLTPERLNAPYLV
CCCCCEEEEEEECCCCCCCCEEECCHHHHHHHHHHHCCCCEEEEEECCCCHHHCCCCEEE
AYEPLREQFFDVHVQCEWLESTEPSYVLYTSGTTGKPKGVQRDVGGYAVALAASMEYIFQ
EEHHHHHHHEEEEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCEEEHHHHHHHHHCC
GKPADTMFTASDVGWVVGHSYIVYAPLIAGLTTVMYEGTPIRPDGGIWWRLVEQYKINLM
CCCCCCEEECCCCCHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHEEEEEEEEEE
FTAPTAIRVLKKQDPALLKQADLSSLRTLFLAGEPLDEPTASWIADSLGKPVVDNFWQTE
EECCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHCCCCC
TGWPILAIQRGVEALPQKLGSPGVPCYGYDLTLRNENTGEPCPPGEKGVITLGYPLPPGC
CCCEEHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCH
MSTVWGDDARFVKTYWESVPNQQVYSTFDWGIQDEDGYVTILGRTDDVINVAGHRLGTRE
HHHHCCCCHHHHHHHHHHCCCCHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHCCCCHHH
IEEALSSHKAVAEVAVVGVTDQVKGQAAIAFVVVRDAAAYDSDDARAKLSADLCATVDRQ
HHHHHHHHHHHHHEEEEECCHHCCCCEEEEEEEEECHHCCCCCCCHHHHHHHHHHHHHHH
LGAIARPAQVALVSMLPKTRSGKLLRRAIAALAEGRDPGDLPTIEDPSALQQIRDAVALG
HHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHC
FGTRGG
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9006051; 10411265; 11677609; 10482501 [H]