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The map label for this gene is purR [H]

Identifier: 186476258

GI number: 186476258

Start: 1695210

End: 1696283

Strand: Direct

Name: purR [H]

Synonym: Bphy_1500

Alternate gene names: 186476258

Gene position: 1695210-1696283 (Clockwise)

Preceding gene: 186476257

Following gene: 186476259

Centisome position: 48.72

GC content: 68.34

Gene sequence:

>1074_bases
ATGACGCCCACGATCAAGGATGTCGCTGCCCACGCGGGCTTTTCGATTGCGACCGTCTCGCGCGCGATCAACGCGCCGCA
CACGGTCAACCCCGTGACCCTCGAAAAAGTGCGTCAATCGATCGACGCGCTCAACTTCCGTCCGAGCCCGCTCGGCCGCC
AGCTGCGCGGTGAGCGTTCGCGCCTGATCGGCGTCGTGCTGCCGACGCTCGCCAATCCCGTGTTCGCCGAATGCCTGCAA
GGCATCGACGAACTCGCATCCGCGCAAGGCTATCGTCTGATGCTGATGACGACGCAATACGACGCCGACCGTGAGCGCCG
CGCGATCGAAACGCTGCGCGAACAGCGCGTGGAAGGCCTGATCCTGACGGTCGCCGACGCCGACACACATCCGCTGCTCG
ACGACCTCGACCGCGACGGCATGCTGTACGTACTGATGCACAACGACACCGTGCGCCGTCCGTCGGTATCCGTCGACAAC
CGCCAGGCCGCGTACGACGGCGTGCGCATGCTGATCGCGCACGGTCATCGGCGCATCCTGATGCTCGCGGGCACGCTCGC
CGCATCGGACCGCGCGCGGCTGCGCCATCTCGGCTACACCCAGGCGATGCAGCAGGCGGGCCTCACGCCCGCGCCCGCGC
TCGAAATCGACTTCAACGCCGACGAGCTCGCGCCCTCCGTGCTCAATCATCTGACCACGGGCGCGAATCGTCCGACGGCC
CTCTTCTGCTCGAACGATCTGCTCGCGATGGTCGTCATGCGCGGCCTGCGCCGCGCACGCTTCGAGATGCCCGGCGACAT
GTCGATTCTCGGCTTCGACGGCCTCGCGATGGGCGAACTGCTGTCGCCGCCGCTCGCGAGCGTCTGCGCGCCGAACCGCG
AGATCGGCTGCGCCGCGTGGCGTCGGCTGATCGAGCGCGTCGACGGCACGCAGCGGTTGGACTTCGACGACGCGCTGCGT
GCACCCTCGCTGTCGCTGACCTTGCCGCACACGCTGCGCGAGGGCGCGACGATCGCCGCGATCTGCGACATCGCGGGCGC
CCCGTCGAACCGCATTGTGCGCACGCTCGCCTGA

Upstream 100 bases:

>100_bases
CGACCCGTCACAAACGTTCCCGATAATCCGGCCTGCGAAAACGTTTACAACACCGCGCGGCGGCAACGCCGCTCGGCACA
GGCTGACGGGAAACCCAACG

Downstream 100 bases:

>100_bases
AGTCATAGAACCCGTTTCAAGGCCGCCAACCATCCTTCTCAACCTTCCGGAGACCTGTCGTGATTCGCCGTTCCTCCATC
GGTATCGCAGCCGTGCGCGT

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MTPTIKDVAAHAGFSIATVSRAINAPHTVNPVTLEKVRQSIDALNFRPSPLGRQLRGERSRLIGVVLPTLANPVFAECLQ
GIDELASAQGYRLMLMTTQYDADRERRAIETLREQRVEGLILTVADADTHPLLDDLDRDGMLYVLMHNDTVRRPSVSVDN
RQAAYDGVRMLIAHGHRRILMLAGTLAASDRARLRHLGYTQAMQQAGLTPAPALEIDFNADELAPSVLNHLTTGANRPTA
LFCSNDLLAMVVMRGLRRARFEMPGDMSILGFDGLAMGELLSPPLASVCAPNREIGCAAWRRLIERVDGTQRLDFDDALR
APSLSLTLPHTLREGATIAAICDIAGAPSNRIVRTLA

Sequences:

>Translated_357_residues
MTPTIKDVAAHAGFSIATVSRAINAPHTVNPVTLEKVRQSIDALNFRPSPLGRQLRGERSRLIGVVLPTLANPVFAECLQ
GIDELASAQGYRLMLMTTQYDADRERRAIETLREQRVEGLILTVADADTHPLLDDLDRDGMLYVLMHNDTVRRPSVSVDN
RQAAYDGVRMLIAHGHRRILMLAGTLAASDRARLRHLGYTQAMQQAGLTPAPALEIDFNADELAPSVLNHLTTGANRPTA
LFCSNDLLAMVVMRGLRRARFEMPGDMSILGFDGLAMGELLSPPLASVCAPNREIGCAAWRRLIERVDGTQRLDFDDALR
APSLSLTLPHTLREGATIAAICDIAGAPSNRIVRTLA
>Mature_356_residues
TPTIKDVAAHAGFSIATVSRAINAPHTVNPVTLEKVRQSIDALNFRPSPLGRQLRGERSRLIGVVLPTLANPVFAECLQG
IDELASAQGYRLMLMTTQYDADRERRAIETLREQRVEGLILTVADADTHPLLDDLDRDGMLYVLMHNDTVRRPSVSVDNR
QAAYDGVRMLIAHGHRRILMLAGTLAASDRARLRHLGYTQAMQQAGLTPAPALEIDFNADELAPSVLNHLTTGANRPTAL
FCSNDLLAMVVMRGLRRARFEMPGDMSILGFDGLAMGELLSPPLASVCAPNREIGCAAWRRLIERVDGTQRLDFDDALRA
PSLSLTLPHTLREGATIAAICDIAGAPSNRIVRTLA

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=314, Percent_Identity=29.2993630573248, Blast_Score=151, Evalue=5e-38,
Organism=Escherichia coli, GI1790194, Length=324, Percent_Identity=32.0987654320988, Blast_Score=148, Evalue=6e-37,
Organism=Escherichia coli, GI48994940, Length=311, Percent_Identity=31.8327974276527, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1790715, Length=314, Percent_Identity=26.7515923566879, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1787948, Length=311, Percent_Identity=27.0096463022508, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1789202, Length=295, Percent_Identity=29.8305084745763, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1786540, Length=319, Percent_Identity=29.7805642633229, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1788474, Length=293, Percent_Identity=28.3276450511945, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1789068, Length=338, Percent_Identity=28.9940828402367, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1787580, Length=320, Percent_Identity=25, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1787906, Length=330, Percent_Identity=28.4848484848485, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1786268, Length=302, Percent_Identity=24.5033112582781, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1790689, Length=309, Percent_Identity=23.6245954692557, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38977; Mature: 38846

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPTIKDVAAHAGFSIATVSRAINAPHTVNPVTLEKVRQSIDALNFRPSPLGRQLRGERS
CCCCHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHCHHH
RLIGVVLPTLANPVFAECLQGIDELASAQGYRLMLMTTQYDADRERRAIETLREQRVEGL
HHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCEE
ILTVADADTHPLLDDLDRDGMLYVLMHNDTVRRPSVSVDNRQAAYDGVRMLIAHGHRRIL
EEEEECCCCCCCHHHCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEE
MLAGTLAASDRARLRHLGYTQAMQQAGLTPAPALEIDFNADELAPSVLNHLTTGANRPTA
EEECHHHHCHHHHHHHCCHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCEE
LFCSNDLLAMVVMRGLRRARFEMPGDMSILGFDGLAMGELLSPPLASVCAPNREIGCAAW
EEECCHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHCCCCHHHHHCCCCCCCHHHH
RRLIERVDGTQRLDFDDALRAPSLSLTLPHTLREGATIAAICDIAGAPSNRIVRTLA
HHHHHHCCCCCCCCHHHHHCCCCCEEECCHHHHCCCEEEEEECCCCCCCCCCHHCCC
>Mature Secondary Structure 
TPTIKDVAAHAGFSIATVSRAINAPHTVNPVTLEKVRQSIDALNFRPSPLGRQLRGERS
CCCHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHCHHH
RLIGVVLPTLANPVFAECLQGIDELASAQGYRLMLMTTQYDADRERRAIETLREQRVEGL
HHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCEE
ILTVADADTHPLLDDLDRDGMLYVLMHNDTVRRPSVSVDNRQAAYDGVRMLIAHGHRRIL
EEEEECCCCCCCHHHCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEE
MLAGTLAASDRARLRHLGYTQAMQQAGLTPAPALEIDFNADELAPSVLNHLTTGANRPTA
EEECHHHHCHHHHHHHCCHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCEE
LFCSNDLLAMVVMRGLRRARFEMPGDMSILGFDGLAMGELLSPPLASVCAPNREIGCAAW
EEECCHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHCCCCHHHHHCCCCCCCHHHH
RRLIERVDGTQRLDFDDALRAPSLSLTLPHTLREGATIAAICDIAGAPSNRIVRTLA
HHHHHHCCCCCCCCHHHHHCCCCCEEECCHHHHCCCEEEEEECCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA