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The map label for this gene is proP [H]

Identifier: 186476249

GI number: 186476249

Start: 1687264

End: 1688733

Strand: Direct

Name: proP [H]

Synonym: Bphy_1491

Alternate gene names: 186476249

Gene position: 1687264-1688733 (Clockwise)

Preceding gene: 186476248

Following gene: 186476257

Centisome position: 48.5

GC content: 65.44

Gene sequence:

>1470_bases
TTGACCGCTTCCAGCAACGGCTTCTGGCGACAAAACAACAACGACAACAGCCTCACACTCGACGACATCACGGTCGTCGA
CAACGCTCTTCTCAAGCGTGCTGTCGGCGCGATGGCGCTCGGCAACGCGATGGAATGGTTCGATTTCGGCGTGTACAGCT
ACATCGCCGTGACCCTCGGCAAAGTGTTCTTCCCGTCGAGCAGTCCGTCAGCCCAGCTGATCGCCACGTTCGGCACGTTC
GCCGCCGCGTTCGTCGTGCGCCCGATCGGCGGCATGGTGTTCGGGCCGCTCGGCGACCGCATCGGCCGTCAGCGCGTGCT
CGCGATGACGATGATCATGATGGCCGTCGGCACGTTCGCGATCGGCCTGATTCCGGATTACGGCTCGATCGGCATTCTCG
CGCCCGCCTTGCTGCTGCTCGCGCGTCTCGTGCAGGGCTTCTCGACGGGCGGCGAATATGGCGGCGCGGCGACCTTCATC
GCCGAGTTCGCAACCGACCGCCGCCGCGGCTTCGCGGGCAGCTTCCTCGAATTCGGCACGCTGGTCGGCTATATCTTCGG
CGCGGGCACGGTGGCCGTGCTGACCGCGTCGCTGTCCCAGGAAGCGCTGCTGTCGTGGGGCTGGCGTGTGCCGTTCTTCA
TCGCGGGTCCGCTGGGCCTCGTCGGCCTGTACATCCGGATGAAGCTCGAGGAAACGCCTGCCTTCAGGAAGGAAGCCGAA
GCGCGCGAGGCCGACGAGAAGTCGCGCCCGAAGCAGAGCTTCATGGAACTGCTCGCGCAGCAATGGAAGCCGCTGCTGCA
ATGCGTTGGCCTCGTGCTGATCTTCAACGTGACCGACTACATGGCGCTGTCGTATCTGCCCAGCTATCTGTCGGCGACGC
TGCACTTCAACGAATCGCACGGCCTGTTCCTCGTGCTGGCCGTGATGGTGCTGATGATGCCGATGACGCTGTACGCAGGC
CACCTGTCGGACACGATCGGCCGCAAGCCCGTCATGCTGCTCGGCTGCGTGGGTCTGTTCGCGCTGTCAATTCCTGCTCT
GCTGTTGATCCGCACGGGCGCCGTGCTGCCCGTGTTCGGCGGTCTGCTGATCCTCGGTGCACTGCTGTCGACCTTCACGG
GCGTGATGCCGTCGTCGCTGCCCGCCCTCTTTCCGACGAAGATCCGCTACGGCGCGCTCGCAATCGGCTTCAACGTGTCG
GTGTCGCTGTTCGGCGGCACGACGCCGCTGGTCGCCGCGTGGCTGGTCGACCGCACCGGCAACGCGATGATGCCCGCGTA
CTACCTGATGGGCGCGTCGATCATCGGTATCGTCTCCGTGCTCGCGCTGCGCGAGACGGCCCGCAAGCCGCTGCCCGGCT
CAGGCCCGTGCGTCGCGACGCGTGCGGAAGCGCACGCCGTGCTGCGTGGCGAACGTGAAGCTGCGGAAATCGAAGAAGCG
TATGCGGCTACCGCAACGGTGCGTGCATAA

Upstream 100 bases:

>100_bases
GCCGGCTTCTGCCACGCCTTTGACCGCTCGCGAAACTGCACTTTCCAGCAATCCGTGGCGGAACGCTTCGCGTTCGGCCG
CAGTCATAGGAGCATTCACC

Downstream 100 bases:

>100_bases
GCGAGCCACACGTCTCGCACAGGACTGAAACGGGTCAGCTTCGGCTGATCCGTTTTTTATGGCGTCAGCGTATGCAACGT
GGCTTCGACGCTTGCACCGT

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MTASSNGFWRQNNNDNSLTLDDITVVDNALLKRAVGAMALGNAMEWFDFGVYSYIAVTLGKVFFPSSSPSAQLIATFGTF
AAAFVVRPIGGMVFGPLGDRIGRQRVLAMTMIMMAVGTFAIGLIPDYGSIGILAPALLLLARLVQGFSTGGEYGGAATFI
AEFATDRRRGFAGSFLEFGTLVGYIFGAGTVAVLTASLSQEALLSWGWRVPFFIAGPLGLVGLYIRMKLEETPAFRKEAE
AREADEKSRPKQSFMELLAQQWKPLLQCVGLVLIFNVTDYMALSYLPSYLSATLHFNESHGLFLVLAVMVLMMPMTLYAG
HLSDTIGRKPVMLLGCVGLFALSIPALLLIRTGAVLPVFGGLLILGALLSTFTGVMPSSLPALFPTKIRYGALAIGFNVS
VSLFGGTTPLVAAWLVDRTGNAMMPAYYLMGASIIGIVSVLALRETARKPLPGSGPCVATRAEAHAVLRGEREAAEIEEA
YAATATVRA

Sequences:

>Translated_489_residues
MTASSNGFWRQNNNDNSLTLDDITVVDNALLKRAVGAMALGNAMEWFDFGVYSYIAVTLGKVFFPSSSPSAQLIATFGTF
AAAFVVRPIGGMVFGPLGDRIGRQRVLAMTMIMMAVGTFAIGLIPDYGSIGILAPALLLLARLVQGFSTGGEYGGAATFI
AEFATDRRRGFAGSFLEFGTLVGYIFGAGTVAVLTASLSQEALLSWGWRVPFFIAGPLGLVGLYIRMKLEETPAFRKEAE
AREADEKSRPKQSFMELLAQQWKPLLQCVGLVLIFNVTDYMALSYLPSYLSATLHFNESHGLFLVLAVMVLMMPMTLYAG
HLSDTIGRKPVMLLGCVGLFALSIPALLLIRTGAVLPVFGGLLILGALLSTFTGVMPSSLPALFPTKIRYGALAIGFNVS
VSLFGGTTPLVAAWLVDRTGNAMMPAYYLMGASIIGIVSVLALRETARKPLPGSGPCVATRAEAHAVLRGEREAAEIEEA
YAATATVRA
>Mature_488_residues
TASSNGFWRQNNNDNSLTLDDITVVDNALLKRAVGAMALGNAMEWFDFGVYSYIAVTLGKVFFPSSSPSAQLIATFGTFA
AAFVVRPIGGMVFGPLGDRIGRQRVLAMTMIMMAVGTFAIGLIPDYGSIGILAPALLLLARLVQGFSTGGEYGGAATFIA
EFATDRRRGFAGSFLEFGTLVGYIFGAGTVAVLTASLSQEALLSWGWRVPFFIAGPLGLVGLYIRMKLEETPAFRKEAEA
READEKSRPKQSFMELLAQQWKPLLQCVGLVLIFNVTDYMALSYLPSYLSATLHFNESHGLFLVLAVMVLMMPMTLYAGH
LSDTIGRKPVMLLGCVGLFALSIPALLLIRTGAVLPVFGGLLILGALLSTFTGVMPSSLPALFPTKIRYGALAIGFNVSV
SLFGGTTPLVAAWLVDRTGNAMMPAYYLMGASIIGIVSVLALRETARKPLPGSGPCVATRAEAHAVLRGEREAAEIEEAY
AATATVRA

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=470, Percent_Identity=48.936170212766, Blast_Score=500, Evalue=1e-142,
Organism=Escherichia coli, GI1788292, Length=436, Percent_Identity=30.045871559633, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1788942, Length=406, Percent_Identity=34.4827586206897, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1789941, Length=430, Percent_Identity=34.1860465116279, Blast_Score=185, Evalue=4e-48,
Organism=Escherichia coli, GI87082231, Length=436, Percent_Identity=25, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87082404, Length=370, Percent_Identity=24.3243243243243, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 52134; Mature: 52003

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASSNGFWRQNNNDNSLTLDDITVVDNALLKRAVGAMALGNAMEWFDFGVYSYIAVTLG
CCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
KVFFPSSSPSAQLIATFGTFAAAFVVRPIGGMVFGPLGDRIGRQRVLAMTMIMMAVGTFA
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHHHHHHHHH
IGLIPDYGSIGILAPALLLLARLVQGFSTGGEYGGAATFIAEFATDRRRGFAGSFLEFGT
HHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
LVGYIFGAGTVAVLTASLSQEALLSWGWRVPFFIAGPLGLVGLYIRMKLEETPAFRKEAE
HHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHEEECCCCCHHHHHH
AREADEKSRPKQSFMELLAQQWKPLLQCVGLVLIFNVTDYMALSYLPSYLSATLHFNESH
HHHCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCC
GLFLVLAVMVLMMPMTLYAGHLSDTIGRKPVMLLGCVGLFALSIPALLLIRTGAVLPVFG
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
GLLILGALLSTFTGVMPSSLPALFPTKIRYGALAIGFNVSVSLFGGTTPLVAAWLVDRTG
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCEEEEECEEEEEEECCCHHHHHHHHHHCCC
NAMMPAYYLMGASIIGIVSVLALRETARKPLPGSGPCVATRAEAHAVLRGEREAAEIEEA
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHCCCHHHHHHHHH
YAATATVRA
HHHHHHCCC
>Mature Secondary Structure 
TASSNGFWRQNNNDNSLTLDDITVVDNALLKRAVGAMALGNAMEWFDFGVYSYIAVTLG
CCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
KVFFPSSSPSAQLIATFGTFAAAFVVRPIGGMVFGPLGDRIGRQRVLAMTMIMMAVGTFA
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHHHHHHHHH
IGLIPDYGSIGILAPALLLLARLVQGFSTGGEYGGAATFIAEFATDRRRGFAGSFLEFGT
HHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
LVGYIFGAGTVAVLTASLSQEALLSWGWRVPFFIAGPLGLVGLYIRMKLEETPAFRKEAE
HHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHEEECCCCCHHHHHH
AREADEKSRPKQSFMELLAQQWKPLLQCVGLVLIFNVTDYMALSYLPSYLSATLHFNESH
HHHCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCC
GLFLVLAVMVLMMPMTLYAGHLSDTIGRKPVMLLGCVGLFALSIPALLLIRTGAVLPVFG
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
GLLILGALLSTFTGVMPSSLPALFPTKIRYGALAIGFNVSVSLFGGTTPLVAAWLVDRTG
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCEEEEECEEEEEEECCCHHHHHHHHHHCCC
NAMMPAYYLMGASIIGIVSVLALRETARKPLPGSGPCVATRAEAHAVLRGEREAAEIEEA
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHCCCHHHHHHHHH
YAATATVRA
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]