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The map label for this gene is fixJ [H]

Identifier: 186476230

GI number: 186476230

Start: 1659205

End: 1659849

Strand: Direct

Name: fixJ [H]

Synonym: Bphy_1472

Alternate gene names: 186476230

Gene position: 1659205-1659849 (Clockwise)

Preceding gene: 186476229

Following gene: 186476231

Centisome position: 47.69

GC content: 63.1

Gene sequence:

>645_bases
ATGAACACCCCAGTTACCACACAGGAAACCGTGTTTGTCGTCGACGACGACGAAGCGGTGCGAGATTCGCTGCGCTGGCT
GCTGGAAGCAAACGGCTACCGCGTGCAGTGTTTCTCCAGCGCCGAGCAGTTCATCGACGCATGGCAGCCGCATCAGCATC
CCGGCCAGATCGCGTGCCTGATCCTCGACGTGCGCATGTCGGGCATGAGCGGCCTCGAACTGCAGGAGCGCCTGATCGCC
GACAACACGCTGCTGCCGATCATCTTCGTGACGGGTCACGGCGACGTGCCGATGGCCGTGTCGACGATGAAGAAGGGCGC
GATGGACTTCATCGAAAAGCCGTTCGACGAAGCCGAACTGCGCAAGCTGGTCGAGCGCATGCTCGACAAGGCGCGCAGCG
AAAGCAGCAGCGTGCAGCAGCAGCGCGCCGCCGCCGAGCGCCTCGGCAAGCTCACGGCACGCGAGCATCAGGTGCTCGAG
CGCATCATCGCCGGCCGCCTGAACAAGCAGATCGCCGACGACCTCGGCATCAGCATCAAGACGGTCGAAGCGCATCGCGC
GAACATCATGGAAAAGCTCAACGTGAACACGGTGGCCGATCTGCTGCGTCTCGCGTTGTCCAACAAGCCGCAGCCCGCGC
AATAA

Upstream 100 bases:

>100_bases
CGGGTGCCACTTTCCATTGCAGTCTGCCTATTGGAGAGCCTGACGGCCCGAGCAACGGCGGGTCCGAGGCGCCGACACCA
CAAACCGTTACGGGAGAGCT

Downstream 100 bases:

>100_bases
GACACGACGCCGTCATCGCGTGATGGCGCGATCCTTGCCCGCGCACCAACGCGCGCGGGCGCATGAGATCGTTGACACCA
TCACGCGGCGCTGCGCTCGC

Product: two component LuxR family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MNTPVTTQETVFVVDDDEAVRDSLRWLLEANGYRVQCFSSAEQFIDAWQPHQHPGQIACLILDVRMSGMSGLELQERLIA
DNTLLPIIFVTGHGDVPMAVSTMKKGAMDFIEKPFDEAELRKLVERMLDKARSESSSVQQQRAAAERLGKLTAREHQVLE
RIIAGRLNKQIADDLGISIKTVEAHRANIMEKLNVNTVADLLRLALSNKPQPAQ

Sequences:

>Translated_214_residues
MNTPVTTQETVFVVDDDEAVRDSLRWLLEANGYRVQCFSSAEQFIDAWQPHQHPGQIACLILDVRMSGMSGLELQERLIA
DNTLLPIIFVTGHGDVPMAVSTMKKGAMDFIEKPFDEAELRKLVERMLDKARSESSSVQQQRAAAERLGKLTAREHQVLE
RIIAGRLNKQIADDLGISIKTVEAHRANIMEKLNVNTVADLLRLALSNKPQPAQ
>Mature_214_residues
MNTPVTTQETVFVVDDDEAVRDSLRWLLEANGYRVQCFSSAEQFIDAWQPHQHPGQIACLILDVRMSGMSGLELQERLIA
DNTLLPIIFVTGHGDVPMAVSTMKKGAMDFIEKPFDEAELRKLVERMLDKARSESSSVQQQRAAAERLGKLTAREHQVLE
RIIAGRLNKQIADDLGISIKTVEAHRANIMEKLNVNTVADLLRLALSNKPQPAQ

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=161, Percent_Identity=33.5403726708075, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI1788222, Length=205, Percent_Identity=26.3414634146341, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1790102, Length=197, Percent_Identity=31.4720812182741, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788550, Length=124, Percent_Identity=28.2258064516129, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI1786747, Length=202, Percent_Identity=23.7623762376238, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI1790860, Length=129, Percent_Identity=29.4573643410853, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1790437, Length=124, Percent_Identity=33.0645161290323, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1789809, Length=141, Percent_Identity=29.7872340425532, Blast_Score=60, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23943; Mature: 23943

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTPVTTQETVFVVDDDEAVRDSLRWLLEANGYRVQCFSSAEQFIDAWQPHQHPGQIACL
CCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCCCCCCEEEE
ILDVRMSGMSGLELQERLIADNTLLPIIFVTGHGDVPMAVSTMKKGAMDFIEKPFDEAEL
EEEEHHCCCCCHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHH
RKLVERMLDKARSESSSVQQQRAAAERLGKLTAREHQVLERIIAGRLNKQIADDLGISIK
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
TVEAHRANIMEKLNVNTVADLLRLALSNKPQPAQ
EHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNTPVTTQETVFVVDDDEAVRDSLRWLLEANGYRVQCFSSAEQFIDAWQPHQHPGQIACL
CCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCCCCCCEEEE
ILDVRMSGMSGLELQERLIADNTLLPIIFVTGHGDVPMAVSTMKKGAMDFIEKPFDEAEL
EEEEHHCCCCCHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHH
RKLVERMLDKARSESSSVQQQRAAAERLGKLTAREHQVLERIIAGRLNKQIADDLGISIK
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
TVEAHRANIMEKLNVNTVADLLRLALSNKPQPAQ
EHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275 [H]