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The map label for this gene is tilS [H]

Identifier: 186476145

GI number: 186476145

Start: 1558863

End: 1560281

Strand: Reverse

Name: tilS [H]

Synonym: Bphy_1387

Alternate gene names: 186476145

Gene position: 1560281-1558863 (Counterclockwise)

Preceding gene: 186476146

Following gene: 186476144

Centisome position: 44.85

GC content: 68.57

Gene sequence:

>1419_bases
GTGACTCTCTCCGCTGACACATCCGCCGACCGCCTCGTTCTCGATGCGGTCGGCGTTGCTTTTGGCGCCTTGCCCGGTGC
TGCGCGTGTCGCGATCGCGTTCAGCGGCGGCGTCGATTCGAGCGTGCTGCTGGACGCAGCGGTGCGGGTCGGCGGCGCGG
CGCGTTGTCTCGCGTTGCATGTCCATCACGGTCTCAGTCCGAACGCCGACGCATGGCTCGCGCATGGCGAGGCGTTTGCG
CGCGAGCGGCGGGTCGAGTTTGCTTCAGCGCGGGTGGACGTCTCGCGCGCGAGCGGCGCGAGCATCGAAGCGGCAGCACG
CGATGCGCGTTATCGCGCGCTCGATGCGCTGTGCGCGCAACATCGCGTGCATACGCTTTGGCTCGCGCAGCATGCGGACG
ACCAGGCGGAAACCGTATTGCTTCAGCTGCTGCGCGGCGCGGGGCTCGCCGGACTCGCGGCGATGGCGCCCGAGTATGTG
CCGCCCGGCGCCGCAATCACGCGCGTGCGCCCGCTGCTGCGTCTGCTGCGCGGCCAACTGGAGCGGTACGCGCGCGCACG
GGATCTGGGCTGGATCGACGATGAATCGAATGCCGACACGCGCTACGCGCGCAATGCATTACGTCACGACGTGCTGCCGG
CTCTCGCGGTGCATTTTCCCGGTTTCCGCGACGCGCTTGCGCGCACGGCGGCGCATGCGGCGTCGGCGCAGCGTTTGCTC
GACGAGTTGGCGCGCATCGATCTACACACGGCGCGTGGCGAAGAAGAGGGCGCGCTCTCGCGCGACGCGCTGCTCGCCCT
CGACGACGAGCGCGCGGCCAATCTGCTGCGTTACTGGATGCGCACGCTCAAATTGCCGGCTGCGTCGACTGCGCGTCTGA
TGGATGCGCTGCGGCAGTTGCGCGAAATCGGCGGCGCACACACGTTGCGTGTCGACCATGCAGGCCAGGCGTTGCGCAGC
TATCGCGGACAGATCTATTGGGAAACGGGCGACAGCGCTGATCCCGCCGACGAAACGGCGCTCTTCGAACGCACCGAAAG
CGTGCTCGCATGGCGGGGCGAAAGCGTGTGGCGCCTGCCGCAGTGGCGCGGCACGTTCGTATTCAGCGACGCGGCGGCCG
ACGCATCCGACGCGATTCCCGTCGACGTCCTCACGCGCGCGCCGCTTGTCGCGCGATCGCGCAGCGGCGGCGAACGCATG
CGCTGCGCCTCGAACGGGCCGGGTCGAACGTTGAAGAATCTCTTTCAGGAGCGCGGCGTACCGTCATGGAAGCGCGACGT
GCCGCTGCTGTTCGCCGGCGACGCGTTGCTGTTCGTGCCGCTGATCGGCGTCAACCAGGCGGCGATCGCCGAACCTTCGC
AACGGCGCAGCGCTCGCTACATCAGGATCGAATGGCGCGAGGATCTCACGCTCGCGTGA

Upstream 100 bases:

>100_bases
CTTCGACCGTCTGATGGCCTACGGCAAGTTCAAGGAAACCACGCCCGGCGCGTAAGCGCGCTAGCGTTCTCATCCGCAGG
CGCCTGGGCGCCGTCACCTC

Downstream 100 bases:

>100_bases
TTCGCCATCCATGCCGGGCCGACGGGACGGCTAGTGTCATCAGCGCGCAACATTGGCCGCAAATCCTTGTCCGACAAGCA
TTTGCAACGGCTTTTGCCCG

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MTLSADTSADRLVLDAVGVAFGALPGAARVAIAFSGGVDSSVLLDAAVRVGGAARCLALHVHHGLSPNADAWLAHGEAFA
RERRVEFASARVDVSRASGASIEAAARDARYRALDALCAQHRVHTLWLAQHADDQAETVLLQLLRGAGLAGLAAMAPEYV
PPGAAITRVRPLLRLLRGQLERYARARDLGWIDDESNADTRYARNALRHDVLPALAVHFPGFRDALARTAAHAASAQRLL
DELARIDLHTARGEEEGALSRDALLALDDERAANLLRYWMRTLKLPAASTARLMDALRQLREIGGAHTLRVDHAGQALRS
YRGQIYWETGDSADPADETALFERTESVLAWRGESVWRLPQWRGTFVFSDAAADASDAIPVDVLTRAPLVARSRSGGERM
RCASNGPGRTLKNLFQERGVPSWKRDVPLLFAGDALLFVPLIGVNQAAIAEPSQRRSARYIRIEWREDLTLA

Sequences:

>Translated_472_residues
MTLSADTSADRLVLDAVGVAFGALPGAARVAIAFSGGVDSSVLLDAAVRVGGAARCLALHVHHGLSPNADAWLAHGEAFA
RERRVEFASARVDVSRASGASIEAAARDARYRALDALCAQHRVHTLWLAQHADDQAETVLLQLLRGAGLAGLAAMAPEYV
PPGAAITRVRPLLRLLRGQLERYARARDLGWIDDESNADTRYARNALRHDVLPALAVHFPGFRDALARTAAHAASAQRLL
DELARIDLHTARGEEEGALSRDALLALDDERAANLLRYWMRTLKLPAASTARLMDALRQLREIGGAHTLRVDHAGQALRS
YRGQIYWETGDSADPADETALFERTESVLAWRGESVWRLPQWRGTFVFSDAAADASDAIPVDVLTRAPLVARSRSGGERM
RCASNGPGRTLKNLFQERGVPSWKRDVPLLFAGDALLFVPLIGVNQAAIAEPSQRRSARYIRIEWREDLTLA
>Mature_471_residues
TLSADTSADRLVLDAVGVAFGALPGAARVAIAFSGGVDSSVLLDAAVRVGGAARCLALHVHHGLSPNADAWLAHGEAFAR
ERRVEFASARVDVSRASGASIEAAARDARYRALDALCAQHRVHTLWLAQHADDQAETVLLQLLRGAGLAGLAAMAPEYVP
PGAAITRVRPLLRLLRGQLERYARARDLGWIDDESNADTRYARNALRHDVLPALAVHFPGFRDALARTAAHAASAQRLLD
ELARIDLHTARGEEEGALSRDALLALDDERAANLLRYWMRTLKLPAASTARLMDALRQLREIGGAHTLRVDHAGQALRSY
RGQIYWETGDSADPADETALFERTESVLAWRGESVWRLPQWRGTFVFSDAAADASDAIPVDVLTRAPLVARSRSGGERMR
CASNGPGRTLKNLFQERGVPSWKRDVPLLFAGDALLFVPLIGVNQAAIAEPSQRRSARYIRIEWREDLTLA

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=441, Percent_Identity=37.4149659863946, Blast_Score=188, Evalue=7e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 51342; Mature: 51210

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSADTSADRLVLDAVGVAFGALPGAARVAIAFSGGVDSSVLLDAAVRVGGAARCLALH
CCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHH
VHHGLSPNADAWLAHGEAFARERRVEFASARVDVSRASGASIEAAARDARYRALDALCAQ
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEHHHCCCCCHHHHHHHHHHHHHHHHHHH
HRVHTLWLAQHADDQAETVLLQLLRGAGLAGLAAMAPEYVPPGAAITRVRPLLRLLRGQL
HHHHHEEEHHCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
ERYARARDLGWIDDESNADTRYARNALRHDVLPALAVHFPGFRDALARTAAHAASAQRLL
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
DELARIDLHTARGEEEGALSRDALLALDDERAANLLRYWMRTLKLPAASTARLMDALRQL
HHHHHHHHHHCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
REIGGAHTLRVDHAGQALRSYRGQIYWETGDSADPADETALFERTESVLAWRGESVWRLP
HHCCCCEEEEECHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEECC
QWRGTFVFSDAAADASDAIPVDVLTRAPLVARSRSGGERMRCASNGPGRTLKNLFQERGV
CCCCCEEEECCCCCCCCCCCHHHHHCCCHHHCCCCCCCCEEECCCCCCHHHHHHHHHCCC
PSWKRDVPLLFAGDALLFVPLIGVNQAAIAEPSQRRSARYIRIEWREDLTLA
CCHHHCCCEEEECCCEEEHHHHCCCHHHHCCCHHHCCCCEEEEEECCCCCCC
>Mature Secondary Structure 
TLSADTSADRLVLDAVGVAFGALPGAARVAIAFSGGVDSSVLLDAAVRVGGAARCLALH
CCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHH
VHHGLSPNADAWLAHGEAFARERRVEFASARVDVSRASGASIEAAARDARYRALDALCAQ
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEHHHCCCCCHHHHHHHHHHHHHHHHHHH
HRVHTLWLAQHADDQAETVLLQLLRGAGLAGLAAMAPEYVPPGAAITRVRPLLRLLRGQL
HHHHHEEEHHCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
ERYARARDLGWIDDESNADTRYARNALRHDVLPALAVHFPGFRDALARTAAHAASAQRLL
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
DELARIDLHTARGEEEGALSRDALLALDDERAANLLRYWMRTLKLPAASTARLMDALRQL
HHHHHHHHHHCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
REIGGAHTLRVDHAGQALRSYRGQIYWETGDSADPADETALFERTESVLAWRGESVWRLP
HHCCCCEEEEECHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEECC
QWRGTFVFSDAAADASDAIPVDVLTRAPLVARSRSGGERMRCASNGPGRTLKNLFQERGV
CCCCCEEEECCCCCCCCCCCHHHHHCCCHHHCCCCCCCCEEECCCCCCHHHHHHHHHCCC
PSWKRDVPLLFAGDALLFVPLIGVNQAAIAEPSQRRSARYIRIEWREDLTLA
CCHHHCCCEEEECCCEEEHHHHCCCHHHHCCCHHHCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA