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The map label for this gene is pucI [H]

Identifier: 186476060

GI number: 186476060

Start: 1460173

End: 1461669

Strand: Reverse

Name: pucI [H]

Synonym: Bphy_1302

Alternate gene names: 186476060

Gene position: 1461669-1460173 (Counterclockwise)

Preceding gene: 186476061

Following gene: 186476059

Centisome position: 42.01

GC content: 59.72

Gene sequence:

>1497_bases
ATGGCTCAGTTCAGTGCAGCACCGAATAGTTCGGCACTTCCCGATTACGAGAGCGGCGCTCAAGGCGGCCATGCGCTGCC
TGCAGGCTACAGCGAACGCCTGTACAACGAAGACCTCGCGCCGTTGAAGCATCAGACGTGGGGCGCCTATAACATCTTCG
CGTTCTGGATGTCGGACGTGCATAGCGTCGGCGGCTACGTGTTCGCAGGCAGCCTGTTCGCACTCGGTCTCACGAGCTGG
CAAGTGCTGATCGCGCTGCTGGTCGGCATTACGATCGTGAACGTGCTGTGCAACATGATCGCGAAGCCGAGCCAGCAGAA
CGGCGTGCCGTATCCCGTTGCGTGTCGCGCCACGTTCGGCGTGCTGGGGGCCAACGTTCCTGCCGTGATCCGTGGTCTCA
TCGCCGTCGCGTGGTACGGCATCCAGACCTATCTGGCATCGAGTGCGCTCGTGATCGTCGTGCTCAAGTTCCTCCCTCAA
CTACTGCCCTATGCCGACGTGCATCATCACGGCTTCATGGGCCTGTCCGCGATCGGCTGGGCCGGGTTCATGCTGCTGTG
GGTGCTGCAGGCACTTGTTTTCTGGAACGGCATGGAGACGATCAAGAAGTTCATCGACTTCGCCGGCCCTGCCGTGTATG
TCGTCATGTTCATTCTGGCCGGCTACATGATCTGGCGTGCGGGCTGGCAGAACATCGGCATCAACCTCGGCGGCGTGAAG
TATCACGGCATGGAAGTCATCCCCGTGATGATCACGGCGATCTCGCTGGTGGTGTCGTACTTCTCGGGTCCGATGCTGAA
TTTCGGCGACTTCTCGCGCTATGGAAAGAGCTTTCGCAGCGTCAAGCGCGGCAACTTCTGGGGGCTGCCCGTCAACTTCC
TCGCGTTCTCGCTGGTGACGGTCGTCACAACGGCCGCGACGCTGCCCGTGTTCGGCGAACTCATTACCGATCCCGTCGAA
ACGGTGGGCCGCATCGATTATCCGACGGCTGTGATTCTCGGCGCACTGACCTTCACGATCGCAACCATCGGCATCAACAT
TGTCGCTAACTTCGTATCGCCTGCATTCGATTTCTCGAACGTTGCGCCGAAGCTCATCAGCTGGCGTGCAGGCGGCATGC
TAGCTGCAGTGGCGTCGATTTTCATCACGCCGTGGAACCTGTTCAATAACCCGGCGGTGATCCACTACACGCTCGATGTG
CTCGGCAGCTTCATCGGACCGTTGTACGGCATCCTGATCGTCGATTACTTCCTCGTGAAGCGTCAGAAGATCGTGCTCGA
CGATCTGTACACCGTATCGGAGAAGGGCAAGTACTGGTATCACAACGGCGTGAATTATCGTGCCGTTGCCGCGCTGCTGC
CGGCTGCAGTGATCGCCGTGATCTGCGTGATGGTGCCGTCGCTGAATGCGCTCGCGAACTTCTCGTGGTTCATTGGCGCG
GGGCTCGGTGCAGTGTTCTACGGCGGACTTGCCCGCAACCTGCAACGTGGTGCATGA

Upstream 100 bases:

>100_bases
GCGCATCACCAAAAGTCACATAGTGGGCTCGCCTCGCGCCTGGCATACACGATGCAGTGCCCGTCGATCGACCTCGACGT
TCCCATCTTAGGAGGAGTTC

Downstream 100 bases:

>100_bases
AGCAGGCATGACAGTTCATCAAAGGCAGCAACGGGAGTCGACATGCGGATCAAACTGATCAACCCGAACACGACGCAACG
GATGACGGAAGCGATGGGCC

Product: cytosine/purines uracil, thiamine, allantoin permease

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MAQFSAAPNSSALPDYESGAQGGHALPAGYSERLYNEDLAPLKHQTWGAYNIFAFWMSDVHSVGGYVFAGSLFALGLTSW
QVLIALLVGITIVNVLCNMIAKPSQQNGVPYPVACRATFGVLGANVPAVIRGLIAVAWYGIQTYLASSALVIVVLKFLPQ
LLPYADVHHHGFMGLSAIGWAGFMLLWVLQALVFWNGMETIKKFIDFAGPAVYVVMFILAGYMIWRAGWQNIGINLGGVK
YHGMEVIPVMITAISLVVSYFSGPMLNFGDFSRYGKSFRSVKRGNFWGLPVNFLAFSLVTVVTTAATLPVFGELITDPVE
TVGRIDYPTAVILGALTFTIATIGINIVANFVSPAFDFSNVAPKLISWRAGGMLAAVASIFITPWNLFNNPAVIHYTLDV
LGSFIGPLYGILIVDYFLVKRQKIVLDDLYTVSEKGKYWYHNGVNYRAVAALLPAAVIAVICVMVPSLNALANFSWFIGA
GLGAVFYGGLARNLQRGA

Sequences:

>Translated_498_residues
MAQFSAAPNSSALPDYESGAQGGHALPAGYSERLYNEDLAPLKHQTWGAYNIFAFWMSDVHSVGGYVFAGSLFALGLTSW
QVLIALLVGITIVNVLCNMIAKPSQQNGVPYPVACRATFGVLGANVPAVIRGLIAVAWYGIQTYLASSALVIVVLKFLPQ
LLPYADVHHHGFMGLSAIGWAGFMLLWVLQALVFWNGMETIKKFIDFAGPAVYVVMFILAGYMIWRAGWQNIGINLGGVK
YHGMEVIPVMITAISLVVSYFSGPMLNFGDFSRYGKSFRSVKRGNFWGLPVNFLAFSLVTVVTTAATLPVFGELITDPVE
TVGRIDYPTAVILGALTFTIATIGINIVANFVSPAFDFSNVAPKLISWRAGGMLAAVASIFITPWNLFNNPAVIHYTLDV
LGSFIGPLYGILIVDYFLVKRQKIVLDDLYTVSEKGKYWYHNGVNYRAVAALLPAAVIAVICVMVPSLNALANFSWFIGA
GLGAVFYGGLARNLQRGA
>Mature_497_residues
AQFSAAPNSSALPDYESGAQGGHALPAGYSERLYNEDLAPLKHQTWGAYNIFAFWMSDVHSVGGYVFAGSLFALGLTSWQ
VLIALLVGITIVNVLCNMIAKPSQQNGVPYPVACRATFGVLGANVPAVIRGLIAVAWYGIQTYLASSALVIVVLKFLPQL
LPYADVHHHGFMGLSAIGWAGFMLLWVLQALVFWNGMETIKKFIDFAGPAVYVVMFILAGYMIWRAGWQNIGINLGGVKY
HGMEVIPVMITAISLVVSYFSGPMLNFGDFSRYGKSFRSVKRGNFWGLPVNFLAFSLVTVVTTAATLPVFGELITDPVET
VGRIDYPTAVILGALTFTIATIGINIVANFVSPAFDFSNVAPKLISWRAGGMLAAVASIFITPWNLFNNPAVIHYTLDVL
GSFIGPLYGILIVDYFLVKRQKIVLDDLYTVSEKGKYWYHNGVNYRAVAALLPAAVIAVICVMVPSLNALANFSWFIGAG
LGAVFYGGLARNLQRGA

Specific function: Transport of allantoin [H]

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=457, Percent_Identity=28.8840262582057, Blast_Score=196, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6319495, Length=434, Percent_Identity=26.036866359447, Blast_Score=154, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6322219, Length=449, Percent_Identity=23.6080178173719, Blast_Score=150, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6319429, Length=438, Percent_Identity=24.6575342465753, Blast_Score=133, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6323266, Length=473, Percent_Identity=23.0443974630021, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324645, Length=471, Percent_Identity=21.8683651804671, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6324766, Length=473, Percent_Identity=21.9873150105708, Blast_Score=105, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 53916; Mature: 53785

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AQFSAAPNSSALPDYESGAQGGHALPAGYSERLYNEDLAPLKHQTWGAYNIFAFWMSDV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHH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HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]