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The map label for this gene is oprM [H]

Identifier: 186476026

GI number: 186476026

Start: 1419328

End: 1420821

Strand: Direct

Name: oprM [H]

Synonym: Bphy_1267

Alternate gene names: 186476026

Gene position: 1419328-1420821 (Clockwise)

Preceding gene: 186476025

Following gene: 186476028

Centisome position: 40.79

GC content: 67.74

Gene sequence:

>1494_bases
ATGCGCCTCTTTTCAATGACCCGCACGTCTTGCGCAGTGGCGGCAGCTTCCCTGTTCGCGACGGCCTGCGCGTTCGGGCC
GAGCGGCAATCCGCCAGCGATGCCCGAACCCGCGCATTACGGCGCCGAAGAACAGTCAACGCAGACAGTGCCCGCGCAAG
GCATCACGCAGCAGTTCGTCGTCGGCGCAAACGCGGTGCCGCAATGGTGGCGCACGTTTGAATCGAATGAACTGAACGCG
CTCGTCGACGAAGGCCTGCGCAACAGCCCCACGCTCGCCGCCGCCGACAGGAATCTGACGGCAGCCCGCGAGCAGTTGCG
GGCGCAGATCGGCAGCAACATGCTGCCCGCCATCGACGCCGGCGGCCAGGCTGCGCGCCAGCGCGCGCTCGGCATTCCCG
AGGCCGGACCGAACACGTTTCTGTACAACACGTTCGTCGGCCAGTTGCAGGCGCAGTACACGTTCGACATCTTCGGCGCG
GCGCGGCTTGCGAACGCGGCGCTGGCCTCGCGTGTGAACGTGCAGGCGTTCCAGTTCAACGCGGCGCGCCGTGCGCTTGC
TGCAAACATTGTGACGGCGGCCGTTTCCGCGGCGATGCTCGATGCCCAGGTGAAGACCACCGAACGCCTCGTCACGCTCG
CCGACGAGCAGGCTCGCGACACGCAGCGCCGCTACGATCTCGGCGCGGTATCGCACAGCGACCTGCTCAATGCGCAGCAA
AGCGCGGCGTCGTTGGCGGCGAGTCTGCCCGCTGCGCGCCAGCAGCTGCTGACGACGCGTCACGCGCTTGCCGTGCTGCT
GGGCAGCACGCCGGACGCCGCGCCGCCGCCGCTCGATCTTGCATCGTTGCATGTGCCCGAACGGGTGCCCGTGTCGGTGC
CGTCCGATTTACTGAAAGCGCGCCCGGACATCGAGGCCGCCGACGCGACGCTGAAGGCAGCCGCGGCCGATGTCGGCGTC
GCGACGGCGCAGATGTATCCGAGCATCACGTTGAACGCGTCGATGGGCCAGGGCGGCTTCAGCTGGCCCGTCGCGCTCTC
GGGCGCGGGCGCGCTCTGGAGCATCGGCGCATCGATCACGCAGCCGATCTTTCACGGCGGCGCATTGTTTGCGCAGCGGC
GCGCGGCGCTCGCTTCGTATGAAGCCGCCACGTCGCAGTACAAGCAAACGGTGCTGACTGCCTTTCAGAACGTCGCCGAT
ACATTGGCTGCGTTGGAACATGACGCGCAATCGCTCGATGCGGCGAATGTGGCCGCGCGCGCGGCGCAACGCGCGTTCGA
AGAGACGTCCGCGAAGTACCGATTGGGCGCCGTCCCCGTCACGTCGACGCGCTCCAGCGAGCAGCAATACCTGAACGCGC
GGCTCGACGAGATTCGCTATACGGGCGCGCGTCTGAACGATACGGCGGCGCTGTTCCAGGCGATGGGCAATCCGCCTGCC
GAGGAGGCGCGCGCTGAGTCGGTGTCGACGGGTGCGGGACCCATTGCACAGTGA

Upstream 100 bases:

>100_bases
CGCTCTTCTATCTGCCCGCGCTCTATGCCGCCTGGTTCCGTGTGCGCGGCAACGAGCGCGATCCGCGCCCGGCGCCGGGC
GAACATTCGGGGAATTGATC

Downstream 100 bases:

>100_bases
GCGACGCGTATCGAGTTATCCCCAGTTTCTGGGGATGATCTTGTTGAGATCGCCGGGACAAGCAGGATAAGTCATTGAGC
CAGCGACGGATTTCTCCCGC

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MRLFSMTRTSCAVAAASLFATACAFGPSGNPPAMPEPAHYGAEEQSTQTVPAQGITQQFVVGANAVPQWWRTFESNELNA
LVDEGLRNSPTLAAADRNLTAAREQLRAQIGSNMLPAIDAGGQAARQRALGIPEAGPNTFLYNTFVGQLQAQYTFDIFGA
ARLANAALASRVNVQAFQFNAARRALAANIVTAAVSAAMLDAQVKTTERLVTLADEQARDTQRRYDLGAVSHSDLLNAQQ
SAASLAASLPAARQQLLTTRHALAVLLGSTPDAAPPPLDLASLHVPERVPVSVPSDLLKARPDIEAADATLKAAAADVGV
ATAQMYPSITLNASMGQGGFSWPVALSGAGALWSIGASITQPIFHGGALFAQRRAALASYEAATSQYKQTVLTAFQNVAD
TLAALEHDAQSLDAANVAARAAQRAFEETSAKYRLGAVPVTSTRSSEQQYLNARLDEIRYTGARLNDTAALFQAMGNPPA
EEARAESVSTGAGPIAQ

Sequences:

>Translated_497_residues
MRLFSMTRTSCAVAAASLFATACAFGPSGNPPAMPEPAHYGAEEQSTQTVPAQGITQQFVVGANAVPQWWRTFESNELNA
LVDEGLRNSPTLAAADRNLTAAREQLRAQIGSNMLPAIDAGGQAARQRALGIPEAGPNTFLYNTFVGQLQAQYTFDIFGA
ARLANAALASRVNVQAFQFNAARRALAANIVTAAVSAAMLDAQVKTTERLVTLADEQARDTQRRYDLGAVSHSDLLNAQQ
SAASLAASLPAARQQLLTTRHALAVLLGSTPDAAPPPLDLASLHVPERVPVSVPSDLLKARPDIEAADATLKAAAADVGV
ATAQMYPSITLNASMGQGGFSWPVALSGAGALWSIGASITQPIFHGGALFAQRRAALASYEAATSQYKQTVLTAFQNVAD
TLAALEHDAQSLDAANVAARAAQRAFEETSAKYRLGAVPVTSTRSSEQQYLNARLDEIRYTGARLNDTAALFQAMGNPPA
EEARAESVSTGAGPIAQ
>Mature_497_residues
MRLFSMTRTSCAVAAASLFATACAFGPSGNPPAMPEPAHYGAEEQSTQTVPAQGITQQFVVGANAVPQWWRTFESNELNA
LVDEGLRNSPTLAAADRNLTAAREQLRAQIGSNMLPAIDAGGQAARQRALGIPEAGPNTFLYNTFVGQLQAQYTFDIFGA
ARLANAALASRVNVQAFQFNAARRALAANIVTAAVSAAMLDAQVKTTERLVTLADEQARDTQRRYDLGAVSHSDLLNAQQ
SAASLAASLPAARQQLLTTRHALAVLLGSTPDAAPPPLDLASLHVPERVPVSVPSDLLKARPDIEAADATLKAAAADVGV
ATAQMYPSITLNASMGQGGFSWPVALSGAGALWSIGASITQPIFHGGALFAQRRAALASYEAATSQYKQTVLTAFQNVAD
TLAALEHDAQSLDAANVAARAAQRAFEETSAKYRLGAVPVTSTRSSEQQYLNARLDEIRYTGARLNDTAALFQAMGNPPA
EEARAESVSTGAGPIAQ

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=469, Percent_Identity=26.4392324093817, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI2367348, Length=442, Percent_Identity=23.7556561085973, Blast_Score=72, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 52055; Mature: 52055

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLFSMTRTSCAVAAASLFATACAFGPSGNPPAMPEPAHYGAEEQSTQTVPAQGITQQFV
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
VGANAVPQWWRTFESNELNALVDEGLRNSPTLAAADRNLTAAREQLRAQIGSNMLPAIDA
HCCCCHHHHHHHHCCCCHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCC
GGQAARQRALGIPEAGPNTFLYNTFVGQLQAQYTFDIFGAARLANAALASRVNVQAFQFN
CCHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHH
AARRALAANIVTAAVSAAMLDAQVKTTERLVTLADEQARDTQRRYDLGAVSHSDLLNAQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
SAASLAASLPAARQQLLTTRHALAVLLGSTPDAAPPPLDLASLHVPERVPVSVPSDLLKA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHC
RPDIEAADATLKAAAADVGVATAQMYPSITLNASMGQGGFSWPVALSGAGALWSIGASIT
CCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEECCCCHHHHHHHHHH
QPIFHGGALFAQRRAALASYEAATSQYKQTVLTAFQNVADTLAALEHDAQSLDAANVAAR
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAQRAFEETSAKYRLGAVPVTSTRSSEQQYLNARLDEIRYTGARLNDTAALFQAMGNPPA
HHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCH
EEARAESVSTGAGPIAQ
HHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRLFSMTRTSCAVAAASLFATACAFGPSGNPPAMPEPAHYGAEEQSTQTVPAQGITQQFV
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
VGANAVPQWWRTFESNELNALVDEGLRNSPTLAAADRNLTAAREQLRAQIGSNMLPAIDA
HCCCCHHHHHHHHCCCCHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCC
GGQAARQRALGIPEAGPNTFLYNTFVGQLQAQYTFDIFGAARLANAALASRVNVQAFQFN
CCHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHH
AARRALAANIVTAAVSAAMLDAQVKTTERLVTLADEQARDTQRRYDLGAVSHSDLLNAQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
SAASLAASLPAARQQLLTTRHALAVLLGSTPDAAPPPLDLASLHVPERVPVSVPSDLLKA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHC
RPDIEAADATLKAAAADVGVATAQMYPSITLNASMGQGGFSWPVALSGAGALWSIGASIT
CCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEECCCCHHHHHHHHHH
QPIFHGGALFAQRRAALASYEAATSQYKQTVLTAFQNVADTLAALEHDAQSLDAANVAAR
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAQRAFEETSAKYRLGAVPVTSTRSSEQQYLNARLDEIRYTGARLNDTAALFQAMGNPPA
HHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCH
EEARAESVSTGAGPIAQ
HHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]