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The map label for this gene is nolF [H]

Identifier: 186476024

GI number: 186476024

Start: 1415016

End: 1416206

Strand: Direct

Name: nolF [H]

Synonym: Bphy_1265

Alternate gene names: 186476024

Gene position: 1415016-1416206 (Clockwise)

Preceding gene: 186476022

Following gene: 186476025

Centisome position: 40.67

GC content: 68.43

Gene sequence:

>1191_bases
GTGAATCGTTCCCGCCGTGGCGCGCCACCTATCGCGCGCAAGCTGTTTCCTCTGTTCGCATCGACTGCACCGGCATCTTC
GTCATCGGGCCGTGCGAACCTGGCCGCGCTCCTTCTCGCCACCGCGTTGACGCTCGCCGCCTGTCACAACAAGGAAGCCG
CCACGCCCGCGCCGAGGCCCGTCGTCGCCGTCGCTGTGCATGCCGATGGCCGGCCGCTCGCTGCATCGCTGCCGGGTGAA
GTGCAGGCGCGTTACTCGACACCGCTGTCATTCCGTATTGCCGGAAAGATCGTCGAGCGGCGCGTGCGCCTCGGCGACGT
CGTGCAGAACGGCCAGGTCGTCGCGCGGCTCGATCCCACCGACGCGCAGAAGAACGCCGCCAGCGCCGCCGCGCAACTCG
ACGCTGCGCAGCATCGGCTCATCTACGCGAAGCAGCAGCTCGAGCGCGACCGTGCGCAGGCGCGCGAGAACCTGATTGCG
CAGACCCAACTGGAGCAGACGGAAGACGCCTACGCATCGGCGGCCGCGCAACGCGACCAGGCGCAGCAGCAGGCCGCGCT
CGCGAAAGACCAGCTGCGGTACACGACGCTCACCGCCGATCACGCGGGCGTCATCACGGCCGAGCAGGCCGATACAGGTC
AGAACGTGTCAGCGGGACAGGCCGTCTACAACCTCGCGTGGTCCGGCGATATCGATGTCGTGTGCGACGTGCCCGAAGCC
GCCCTCGCGTCGCTGCGCGTCGGCACGACGGCGACCGTGAAGCTCGGCGCACTGCCCGGCCGCACGTTCAATGCGCGCGT
GCGTGAACTGTCGCCCGCTGCCGATCCGCAAAGCCGCACCTATCGCGCGAAGCTCACGCTCGATCATGCGGGCGCGGACG
TGCGTCTCGGCATGACAGCCGACATCGACTTCGCACACGACAACACCGCCCCGCACGCCAGCCTCTACACGCTGCCCGCC
ACCGCCCTGTTTCATGACGGCACCCAGCCCGCCGTGTGGGTCGTGAAGAGCGGCAGCGACGAACTGGCACTGCGCCGCGT
GCAGGTCTCGCAATACACAGAGCGGACCATCGCCGTCTCGCAAGGTCTGAGCGAAGGCGAGCGCGTCGTCTGGCAAGGCG
TGCATACGGTGACGGCGGGCGAGAAAGTCCGCGTCGTGGCGCCGCTGCATCCCGAGGATTTCGCGTCATGA

Upstream 100 bases:

>100_bases
CGATGCGCCTCGAATCGAAGTATGCGCGTTGACGCGTCGTCGCATCCCGTCGGTAGCCTCTGGCCTGCCATATCCGTTTG
CATCTCAGCCTGGGGGCATC

Downstream 100 bases:

>100_bases
GCGGCATGCGCGACGAAGAAGGACGCTTCAACCTGTCCGCGTGGGCGCTGCGCCATCAGGCGCTGGTGGTGTTTCTGATC
GCGCTCGCGACGGCGTTCGG

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MNRSRRGAPPIARKLFPLFASTAPASSSSGRANLAALLLATALTLAACHNKEAATPAPRPVVAVAVHADGRPLAASLPGE
VQARYSTPLSFRIAGKIVERRVRLGDVVQNGQVVARLDPTDAQKNAASAAAQLDAAQHRLIYAKQQLERDRAQARENLIA
QTQLEQTEDAYASAAAQRDQAQQQAALAKDQLRYTTLTADHAGVITAEQADTGQNVSAGQAVYNLAWSGDIDVVCDVPEA
ALASLRVGTTATVKLGALPGRTFNARVRELSPAADPQSRTYRAKLTLDHAGADVRLGMTADIDFAHDNTAPHASLYTLPA
TALFHDGTQPAVWVVKSGSDELALRRVQVSQYTERTIAVSQGLSEGERVVWQGVHTVTAGEKVRVVAPLHPEDFAS

Sequences:

>Translated_396_residues
MNRSRRGAPPIARKLFPLFASTAPASSSSGRANLAALLLATALTLAACHNKEAATPAPRPVVAVAVHADGRPLAASLPGE
VQARYSTPLSFRIAGKIVERRVRLGDVVQNGQVVARLDPTDAQKNAASAAAQLDAAQHRLIYAKQQLERDRAQARENLIA
QTQLEQTEDAYASAAAQRDQAQQQAALAKDQLRYTTLTADHAGVITAEQADTGQNVSAGQAVYNLAWSGDIDVVCDVPEA
ALASLRVGTTATVKLGALPGRTFNARVRELSPAADPQSRTYRAKLTLDHAGADVRLGMTADIDFAHDNTAPHASLYTLPA
TALFHDGTQPAVWVVKSGSDELALRRVQVSQYTERTIAVSQGLSEGERVVWQGVHTVTAGEKVRVVAPLHPEDFAS
>Mature_396_residues
MNRSRRGAPPIARKLFPLFASTAPASSSSGRANLAALLLATALTLAACHNKEAATPAPRPVVAVAVHADGRPLAASLPGE
VQARYSTPLSFRIAGKIVERRVRLGDVVQNGQVVARLDPTDAQKNAASAAAQLDAAQHRLIYAKQQLERDRAQARENLIA
QTQLEQTEDAYASAAAQRDQAQQQAALAKDQLRYTTLTADHAGVITAEQADTGQNVSAGQAVYNLAWSGDIDVVCDVPEA
ALASLRVGTTATVKLGALPGRTFNARVRELSPAADPQSRTYRAKLTLDHAGADVRLGMTADIDFAHDNTAPHASLYTLPA
TALFHDGTQPAVWVVKSGSDELALRRVQVSQYTERTIAVSQGLSEGERVVWQGVHTVTAGEKVRVVAPLHPEDFAS

Specific function: Involved in the production of Medicago-specific nodulation signal molecule [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786787, Length=189, Percent_Identity=29.6296296296296, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42057; Mature: 42057

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRSRRGAPPIARKLFPLFASTAPASSSSGRANLAALLLATALTLAACHNKEAATPAPRP
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
VVAVAVHADGRPLAASLPGEVQARYSTPLSFRIAGKIVERRVRLGDVVQNGQVVARLDPT
EEEEEEECCCCEEEECCCCCEEEEECCCEEEEHHHHHHHHHHHHHHHHCCCEEEEEECCC
DAQKNAASAAAQLDAAQHRLIYAKQQLERDRAQARENLIAQTQLEQTEDAYASAAAQRDQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQQQAALAKDQLRYTTLTADHAGVITAEQADTGQNVSAGQAVYNLAWSGDIDVVCDVPEA
HHHHHHHHHHHEEEEEEECCCCCEEEECCCCCCCCCCCCCEEEEEEECCCCEEEECCCHH
ALASLRVGTTATVKLGALPGRTFNARVRELSPAADPQSRTYRAKLTLDHAGADVRLGMTA
HHHHEECCCEEEEEECCCCCCCCCCHHHHCCCCCCCCCCEEEEEEEEECCCCEEEEEEEE
DIDFAHDNTAPHASLYTLPATALFHDGTQPAVWVVKSGSDELALRRVQVSQYTERTIAVS
EEEECCCCCCCCCEEEECCCHHEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
QGLSEGERVVWQGVHTVTAGEKVRVVAPLHPEDFAS
CCCCCCCEEEEECCEEEECCCEEEEEECCCCHHCCC
>Mature Secondary Structure
MNRSRRGAPPIARKLFPLFASTAPASSSSGRANLAALLLATALTLAACHNKEAATPAPRP
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
VVAVAVHADGRPLAASLPGEVQARYSTPLSFRIAGKIVERRVRLGDVVQNGQVVARLDPT
EEEEEEECCCCEEEECCCCCEEEEECCCEEEEHHHHHHHHHHHHHHHHCCCEEEEEECCC
DAQKNAASAAAQLDAAQHRLIYAKQQLERDRAQARENLIAQTQLEQTEDAYASAAAQRDQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQQQAALAKDQLRYTTLTADHAGVITAEQADTGQNVSAGQAVYNLAWSGDIDVVCDVPEA
HHHHHHHHHHHEEEEEEECCCCCEEEECCCCCCCCCCCCCEEEEEEECCCCEEEECCCHH
ALASLRVGTTATVKLGALPGRTFNARVRELSPAADPQSRTYRAKLTLDHAGADVRLGMTA
HHHHEECCCEEEEEECCCCCCCCCCHHHHCCCCCCCCCCEEEEEEEEECCCCEEEEEEEE
DIDFAHDNTAPHASLYTLPATALFHDGTQPAVWVVKSGSDELALRRVQVSQYTERTIAVS
EEEECCCCCCCCCEEEECCCHHEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
QGLSEGERVVWQGVHTVTAGEKVRVVAPLHPEDFAS
CCCCCCCEEEEECCEEEECCCEEEEEECCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1909418; 11481432 [H]