Click here to switch to the map view.

The map label for this gene is nreB [H]

Identifier: 186475857

GI number: 186475857

Start: 1233043

End: 1234524

Strand: Reverse

Name: nreB [H]

Synonym: Bphy_1092

Alternate gene names: 186475857

Gene position: 1234524-1233043 (Counterclockwise)

Preceding gene: 186475858

Following gene: 186475856

Centisome position: 35.48

GC content: 60.53

Gene sequence:

>1482_bases
ATGAGCGCACCAGGCACGAGTGACGCGAATCCGGTGCCGCAAGGCAATCAATCCGGCGAACACCGCTATCACTTCGCCAT
CGACATCGACGGGGAATTTGCCGTCTTTACGCTCGACACGCACGGTCAGGTCGAGACATGGAATGCGTCAGCCGAACGCA
TCACAGGATATGGCGCGGGTGAAATTCTTGGTAAGCCGCTTTCGGTACTCTACCCGACGGTTTCCGCGGGTGTGCCCGTT
CATTCGGACATCCTGGTGCGAGCAGCGACGACTGGGCGGGCCTATGACGAGGGCTGGCACGCGCGCAAGGACGGAATACG
GTTCTGGGCCAGCGTACTCGTCATTGCTCAGCAACGTGAGCAGGGCGATCTGGCTGGTTTTGCAGCCGTCACGCACGATC
TTTCGCAGCGTCAACTGATCGAGGCAAAGCTTCGCAGAAGCGAGCAGCGATTACAGGCGATGATTGACAGCGTCGAAGAC
TACGCGATCTTCATGCTCAGTCCGACGGGCGAGGTCACCTCCTGGAATACGGGAGCCCAGCGAATCGAGGGCTATTGCGC
AGGTGAAATTATCGGCCGCCATTTTTCTGTCTTCTATCCGCCAGAAGCGCGGACGGCGGGTTTGCCCGAACGCATGCTCG
AGACGGCATCCACGCAGGGCCGCTCCGAAGCCGAAGGATGGCGTGTCAGAAGTGATGGGTCGCGTTTCTGGGCGGACGCT
ATTATCACGCCCATTCGCGACGACAAGGGGGCGCTGCAGGGTTTCGCCAAAGTGACGCGTGATCTGACCGAGCATAAACG
GCTGGAACAACTCGAACATGTCGGCGAACTCGCTGCGGCGACGGAGCGGGCGCGGGAAGAGGAAAAGAAGAGAATCGCCC
GGGAACTGCACGATGACCTCGGTCAGCAGTTGACCGCATTGAAAATGAGCGCCGTCATCCTGCAGCAGGACGTCGAAGCA
GGGCATTACACCGCGGAGACAGCCGCGAAGGCGCGTGGCCTGGTCGTACAGGCGGACGATACGATGAGCGCGTTGCGACG
CATCGCGTCGAATCTGCGTCCCACGATGCTCGACGATCTGGGGTTGCTGCCTGCCGTAGAGTGGCTCGCGGATGAATTTA
GTCGGCGCTACGGGATCAAGGCCAGCACCCGTTTGTCGGCGGGCGAGCTCGCGTTCAGCGATACGGCATCAACCGCAATC
TTTCGCATCGTTCAGGAGGCGCTGACAAACGTGGCGCGTCATGCGTGTGCGACGGAAGTCGTCGTCACCATCGAGCTCGA
CGATAACGCGTGTGAAGTTCGCATCGATGACAACGGACGCGGGGCGGCTTCCGCGCCCGGCGACAAGCCCGACTCTTTTG
GCCTGCTGGGCATACGCGAGCGCGTCCGGCAACTTAACGGCACCGTGTCGCTCGGAAATCGACTGGACAGAGGATTTCGT
GTCCAGATCAGGTTTCCGATCGACACCATCGTCCGTCATTGA

Upstream 100 bases:

>100_bases
TGAATGACTTCCCGCTGTGCCCACGTAGGGCCGGCCGGTGTTTTCCGGCCGGTCGAAGAACCAGACTCGAAGTTGATGAC
GGGAGGCGCCTGTGAGCAGC

Downstream 100 bases:

>100_bases
GGACGCCTTTCGGCAACGTCGCACCCGCATTGATGCAAATCAAGCGAGCCACGCCTGCTTGCTCCTATCGTAAGTTTGCC
CGTAGCCGTTCTGGTTTCAC

Product: PAS/PAC sensor signal transduction histidine kinase

Products: NA

Alternate protein names: Nitrogen regulation protein B [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MSAPGTSDANPVPQGNQSGEHRYHFAIDIDGEFAVFTLDTHGQVETWNASAERITGYGAGEILGKPLSVLYPTVSAGVPV
HSDILVRAATTGRAYDEGWHARKDGIRFWASVLVIAQQREQGDLAGFAAVTHDLSQRQLIEAKLRRSEQRLQAMIDSVED
YAIFMLSPTGEVTSWNTGAQRIEGYCAGEIIGRHFSVFYPPEARTAGLPERMLETASTQGRSEAEGWRVRSDGSRFWADA
IITPIRDDKGALQGFAKVTRDLTEHKRLEQLEHVGELAAATERAREEEKKRIARELHDDLGQQLTALKMSAVILQQDVEA
GHYTAETAAKARGLVVQADDTMSALRRIASNLRPTMLDDLGLLPAVEWLADEFSRRYGIKASTRLSAGELAFSDTASTAI
FRIVQEALTNVARHACATEVVVTIELDDNACEVRIDDNGRGAASAPGDKPDSFGLLGIRERVRQLNGTVSLGNRLDRGFR
VQIRFPIDTIVRH

Sequences:

>Translated_493_residues
MSAPGTSDANPVPQGNQSGEHRYHFAIDIDGEFAVFTLDTHGQVETWNASAERITGYGAGEILGKPLSVLYPTVSAGVPV
HSDILVRAATTGRAYDEGWHARKDGIRFWASVLVIAQQREQGDLAGFAAVTHDLSQRQLIEAKLRRSEQRLQAMIDSVED
YAIFMLSPTGEVTSWNTGAQRIEGYCAGEIIGRHFSVFYPPEARTAGLPERMLETASTQGRSEAEGWRVRSDGSRFWADA
IITPIRDDKGALQGFAKVTRDLTEHKRLEQLEHVGELAAATERAREEEKKRIARELHDDLGQQLTALKMSAVILQQDVEA
GHYTAETAAKARGLVVQADDTMSALRRIASNLRPTMLDDLGLLPAVEWLADEFSRRYGIKASTRLSAGELAFSDTASTAI
FRIVQEALTNVARHACATEVVVTIELDDNACEVRIDDNGRGAASAPGDKPDSFGLLGIRERVRQLNGTVSLGNRLDRGFR
VQIRFPIDTIVRH
>Mature_492_residues
SAPGTSDANPVPQGNQSGEHRYHFAIDIDGEFAVFTLDTHGQVETWNASAERITGYGAGEILGKPLSVLYPTVSAGVPVH
SDILVRAATTGRAYDEGWHARKDGIRFWASVLVIAQQREQGDLAGFAAVTHDLSQRQLIEAKLRRSEQRLQAMIDSVEDY
AIFMLSPTGEVTSWNTGAQRIEGYCAGEIIGRHFSVFYPPEARTAGLPERMLETASTQGRSEAEGWRVRSDGSRFWADAI
ITPIRDDKGALQGFAKVTRDLTEHKRLEQLEHVGELAAATERAREEEKKRIARELHDDLGQQLTALKMSAVILQQDVEAG
HYTAETAAKARGLVVQADDTMSALRRIASNLRPTMLDDLGLLPAVEWLADEFSRRYGIKASTRLSAGELAFSDTASTAIF
RIVQEALTNVARHACATEVVVTIELDDNACEVRIDDNGRGAASAPGDKPDSFGLLGIRERVRQLNGTVSLGNRLDRGFRV
QIRFPIDTIVRH

Specific function: Member of the two-component regulatory system nreB/nreC involved in the control of dissimilatory nitrate/nitrite reduction in response to oxygen. NreB functions as a direct oxygen sensor histidine kinase which is autophosphorylated, in the absence of oxyg

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=203, Percent_Identity=29.064039408867, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI87082311, Length=245, Percent_Identity=29.7959183673469, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1788812, Length=202, Percent_Identity=29.2079207920792, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI87081921, Length=253, Percent_Identity=22.9249011857708, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017203 [H]

Pfam domain/function: PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 54156; Mature: 54025

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPGTSDANPVPQGNQSGEHRYHFAIDIDGEFAVFTLDTHGQVETWNASAERITGYGAG
CCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCEEEEEEECCCCEEEECCCHHHHCCCCCH
EILGKPLSVLYPTVSAGVPVHSDILVRAATTGRAYDEGWHARKDGIRFWASVLVIAQQRE
HHHCCCHHHHCCHHHCCCCCCCCEEEEEECCCCCHHCCCHHHHHHHHHHHHHHHHHHHCC
QGDLAGFAAVTHDLSQRQLIEAKLRRSEQRLQAMIDSVEDYAIFMLSPTGEVTSWNTGAQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHH
RIEGYCAGEIIGRHFSVFYPPEARTAGLPERMLETASTQGRSEAEGWRVRSDGSRFWADA
HHCCHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHH
IITPIRDDKGALQGFAKVTRDLTEHKRLEQLEHVGELAAATERAREEEKKRIARELHDDL
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GQQLTALKMSAVILQQDVEAGHYTAETAAKARGLVVQADDTMSALRRIASNLRPTMLDDL
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCEEEECCHHHHHHHHHHHCCCCHHHHHC
GLLPAVEWLADEFSRRYGIKASTRLSAGELAFSDTASTAIFRIVQEALTNVARHACATEV
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHEEE
VVTIELDDNACEVRIDDNGRGAASAPGDKPDSFGLLGIRERVRQLNGTVSLGNRLDRGFR
EEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEHHHHCCCCEE
VQIRFPIDTIVRH
EEEECCHHHHHCC
>Mature Secondary Structure 
SAPGTSDANPVPQGNQSGEHRYHFAIDIDGEFAVFTLDTHGQVETWNASAERITGYGAG
CCCCCCCCCCCCCCCCCCCEEEEEEEEECCCEEEEEEECCCCEEEECCCHHHHCCCCCH
EILGKPLSVLYPTVSAGVPVHSDILVRAATTGRAYDEGWHARKDGIRFWASVLVIAQQRE
HHHCCCHHHHCCHHHCCCCCCCCEEEEEECCCCCHHCCCHHHHHHHHHHHHHHHHHHHCC
QGDLAGFAAVTHDLSQRQLIEAKLRRSEQRLQAMIDSVEDYAIFMLSPTGEVTSWNTGAQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHH
RIEGYCAGEIIGRHFSVFYPPEARTAGLPERMLETASTQGRSEAEGWRVRSDGSRFWADA
HHCCHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHH
IITPIRDDKGALQGFAKVTRDLTEHKRLEQLEHVGELAAATERAREEEKKRIARELHDDL
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GQQLTALKMSAVILQQDVEAGHYTAETAAKARGLVVQADDTMSALRRIASNLRPTMLDDL
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCEEEECCHHHHHHHHHHHCCCCHHHHHC
GLLPAVEWLADEFSRRYGIKASTRLSAGELAFSDTASTAIFRIVQEALTNVARHACATEV
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHEEE
VVTIELDDNACEVRIDDNGRGAASAPGDKPDSFGLLGIRERVRQLNGTVSLGNRLDRGFR
EEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEHHHHCCCCEE
VQIRFPIDTIVRH
EEEECCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12426351 [H]