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The map label for this gene is thlA [H]

Identifier: 186475740

GI number: 186475740

Start: 1090012

End: 1091196

Strand: Direct

Name: thlA [H]

Synonym: Bphy_0975

Alternate gene names: 186475740

Gene position: 1090012-1091196 (Clockwise)

Preceding gene: 186475739

Following gene: 186475741

Centisome position: 31.33

GC content: 65.82

Gene sequence:

>1185_bases
ATGCAACGTGAAGTAGTGGTAGTGAGCGGTGTCCGCACAGCCATCGGCGACTTCGGCGGCAGCCTGAAAGACTTCGCGCC
GACGGATCTCGGTGCGCGTGTCGTACGCGAGGCCTTGTCGCGGGCGAACGTCGCCGGCGACGAAGTCGGCCATGTCGTGT
TCGGCAGCGTGATCCACACCGAACCGAAGGACATGTACCTCGCGCGCGTCGCGGCCATCAATGGCGGCGTGTCGCAGCAC
GCGCCCGCGCTGACCGTGAACCGGCTGTGCGGCTCGGGCCTGCAGGCAATCGTGTCGGCCGCGCAGTCGGTGCTGCTAGG
CGATACGGACGTCGCGATCGGCGCAGGCGCGGAAAGCATGAGCCGCGCGCCGTACATCATGCCGTCGGCGCGTTTCGGCC
AGCGCATGGGCAACGCGAGCATCGTCGACATGATGCTCGGCGCGCTGCACGATCCGTTTCAGACGATCCACATGGGCGTG
ACGGCCGAAAACGTCGCGAAGAAGTACGACATCTCGCGCGACGCGCAAGACGCACTCGCGCTCGAATCGCACCATCGCGC
GGCCAAGGCGATTGAAGCCGGCTACTTCAAGGAACAGATTCTGCCCATCACGATTGCGTCGAAGAAGGGCGACACCGTGT
TCGACACGGACGAGCACGTGCGCCTGAACGCCACCGCCGACGATTTTTCGAAACTGAAACCCGTGTTCGCGAAGGAAAAC
GGCACGGTGACGGCGGGCAACGCGTCGGGCATCAACGACGCGGCGGCGGCCGTCGTGCTGATGGAGCGCAGCGTCGCCGA
AAAGCGCGGCATGAAGCCGCTCGGCAGGCTGGTCGCTTACGCGCATGCGGGCGTCGACCCGAACTACATGGGCATCGGCC
CCGTGCCCGCGACGCAGAAGGTGCTCGAACGCGCTGGCCTGACGGTCGCGGATCTCGACGTGATCGAGGCGAACGAAGCG
TTTGCCGCGCAGGCTTGCGCCGTCAGCAAGGAACTCGGCCTCGATCCCGCGAAGGTGAACCCGAACGGCTCGGGCATTTC
GCTCGGCCACCCCATCGGCGCGACGGGCGCGCTGATCACCGTGAAGGCGTTGTATGAGCTGCAGCGCATCGGCGGACGTT
ATGCGCTCGTGACGATGTGTATCGGCGGCGGGCAAGGCATCGCCGCGATCTTCGAGCGGATTTGA

Upstream 100 bases:

>100_bases
CGCGCGCACATCCGGCAACGCCGGTAGTGCAAAGGCAAGATCACACGCGGCGGCAACGCCGCGCGCGATATCCAACTGAG
ACAAAGAAGAGGAGCAAGCG

Downstream 100 bases:

>100_bases
TCCGCTCATCCGGGACAGGGCGGCCGCCGCGCCGCCCCGTTGCTGAACGAACTTACCGGTCGAAGGGGGTTCGCGACGCC
GTGCGCACGAGGCGGCATCG

Product: beta-ketothiolase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MQREVVVVSGVRTAIGDFGGSLKDFAPTDLGARVVREALSRANVAGDEVGHVVFGSVIHTEPKDMYLARVAAINGGVSQH
APALTVNRLCGSGLQAIVSAAQSVLLGDTDVAIGAGAESMSRAPYIMPSARFGQRMGNASIVDMMLGALHDPFQTIHMGV
TAENVAKKYDISRDAQDALALESHHRAAKAIEAGYFKEQILPITIASKKGDTVFDTDEHVRLNATADDFSKLKPVFAKEN
GTVTAGNASGINDAAAAVVLMERSVAEKRGMKPLGRLVAYAHAGVDPNYMGIGPVPATQKVLERAGLTVADLDVIEANEA
FAAQACAVSKELGLDPAKVNPNGSGISLGHPIGATGALITVKALYELQRIGGRYALVTMCIGGGQGIAAIFERI

Sequences:

>Translated_394_residues
MQREVVVVSGVRTAIGDFGGSLKDFAPTDLGARVVREALSRANVAGDEVGHVVFGSVIHTEPKDMYLARVAAINGGVSQH
APALTVNRLCGSGLQAIVSAAQSVLLGDTDVAIGAGAESMSRAPYIMPSARFGQRMGNASIVDMMLGALHDPFQTIHMGV
TAENVAKKYDISRDAQDALALESHHRAAKAIEAGYFKEQILPITIASKKGDTVFDTDEHVRLNATADDFSKLKPVFAKEN
GTVTAGNASGINDAAAAVVLMERSVAEKRGMKPLGRLVAYAHAGVDPNYMGIGPVPATQKVLERAGLTVADLDVIEANEA
FAAQACAVSKELGLDPAKVNPNGSGISLGHPIGATGALITVKALYELQRIGGRYALVTMCIGGGQGIAAIFERI
>Mature_394_residues
MQREVVVVSGVRTAIGDFGGSLKDFAPTDLGARVVREALSRANVAGDEVGHVVFGSVIHTEPKDMYLARVAAINGGVSQH
APALTVNRLCGSGLQAIVSAAQSVLLGDTDVAIGAGAESMSRAPYIMPSARFGQRMGNASIVDMMLGALHDPFQTIHMGV
TAENVAKKYDISRDAQDALALESHHRAAKAIEAGYFKEQILPITIASKKGDTVFDTDEHVRLNATADDFSKLKPVFAKEN
GTVTAGNASGINDAAAAVVLMERSVAEKRGMKPLGRLVAYAHAGVDPNYMGIGPVPATQKVLERAGLTVADLDVIEANEA
FAAQACAVSKELGLDPAKVNPNGSGISLGHPIGATGALITVKALYELQRIGGRYALVTMCIGGGQGIAAIFERI

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=393, Percent_Identity=44.529262086514, Blast_Score=345, Evalue=3e-95,
Organism=Homo sapiens, GI148539872, Length=391, Percent_Identity=44.5012787723785, Blast_Score=339, Evalue=2e-93,
Organism=Homo sapiens, GI4557237, Length=397, Percent_Identity=40.8060453400504, Blast_Score=271, Evalue=7e-73,
Organism=Homo sapiens, GI4501853, Length=397, Percent_Identity=41.5617128463476, Blast_Score=269, Evalue=3e-72,
Organism=Homo sapiens, GI4504327, Length=425, Percent_Identity=36.2352941176471, Blast_Score=204, Evalue=2e-52,
Organism=Homo sapiens, GI194353979, Length=391, Percent_Identity=30.9462915601023, Blast_Score=156, Evalue=3e-38,
Organism=Escherichia coli, GI1788554, Length=398, Percent_Identity=48.2412060301507, Blast_Score=343, Evalue=1e-95,
Organism=Escherichia coli, GI87082165, Length=392, Percent_Identity=45.4081632653061, Blast_Score=330, Evalue=8e-92,
Organism=Escherichia coli, GI1787663, Length=404, Percent_Identity=45.7920792079208, Blast_Score=323, Evalue=1e-89,
Organism=Escherichia coli, GI48994986, Length=400, Percent_Identity=38.25, Blast_Score=248, Evalue=4e-67,
Organism=Escherichia coli, GI1788683, Length=410, Percent_Identity=35.3658536585366, Blast_Score=218, Evalue=6e-58,
Organism=Caenorhabditis elegans, GI133906874, Length=391, Percent_Identity=46.2915601023018, Blast_Score=355, Evalue=2e-98,
Organism=Caenorhabditis elegans, GI25147385, Length=393, Percent_Identity=34.8600508905852, Blast_Score=239, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI17535921, Length=396, Percent_Identity=32.8282828282828, Blast_Score=219, Evalue=3e-57,
Organism=Caenorhabditis elegans, GI17551802, Length=423, Percent_Identity=30.9692671394799, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17535917, Length=396, Percent_Identity=26.7676767676768, Blast_Score=149, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6325229, Length=401, Percent_Identity=38.1546134663342, Blast_Score=259, Evalue=6e-70,
Organism=Saccharomyces cerevisiae, GI6322031, Length=406, Percent_Identity=37.6847290640394, Blast_Score=233, Evalue=4e-62,
Organism=Drosophila melanogaster, GI24655093, Length=392, Percent_Identity=45.1530612244898, Blast_Score=351, Evalue=4e-97,
Organism=Drosophila melanogaster, GI17648125, Length=394, Percent_Identity=42.8934010152284, Blast_Score=308, Evalue=4e-84,
Organism=Drosophila melanogaster, GI24640423, Length=394, Percent_Identity=40.1015228426396, Blast_Score=271, Evalue=8e-73,
Organism=Drosophila melanogaster, GI17137578, Length=426, Percent_Identity=32.1596244131455, Blast_Score=186, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 41037; Mature: 41037

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00098 THIOLASE_1 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQREVVVVSGVRTAIGDFGGSLKDFAPTDLGARVVREALSRANVAGDEVGHVVFGSVIHT
CCCCEEEECCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHCC
EPKDMYLARVAAINGGVSQHAPALTVNRLCGSGLQAIVSAAQSVLLGDTDVAIGAGAESM
CCHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEECCHHHH
SRAPYIMPSARFGQRMGNASIVDMMLGALHDPFQTIHMGVTAENVAKKYDISRDAQDALA
CCCCEECCCHHHHHHCCCHHHHHHHHHHHCCCHHHHEECCCHHHHHHHHCCCCCHHHHHH
LESHHRAAKAIEAGYFKEQILPITIASKKGDTVFDTDEHVRLNATADDFSKLKPVFAKEN
HHHHHHHHHHHHHHHHHHCEEEEEEECCCCCEEECCCCCEEEECCHHHHHHCCCCEECCC
GTVTAGNASGINDAAAAVVLMERSVAEKRGMKPLGRLVAYAHAGVDPNYMGIGPVPATQK
CEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHH
VLERAGLTVADLDVIEANEAFAAQACAVSKELGLDPAKVNPNGSGISLGHPIGATGALIT
HHHHCCCEEEEEEEEECCHHHHHHHHHHHHHHCCCCCEECCCCCCEECCCCCCCCHHHHH
VKALYELQRIGGRYALVTMCIGGGQGIAAIFERI
HHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHCC
>Mature Secondary Structure
MQREVVVVSGVRTAIGDFGGSLKDFAPTDLGARVVREALSRANVAGDEVGHVVFGSVIHT
CCCCEEEECCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHCC
EPKDMYLARVAAINGGVSQHAPALTVNRLCGSGLQAIVSAAQSVLLGDTDVAIGAGAESM
CCHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEECCHHHH
SRAPYIMPSARFGQRMGNASIVDMMLGALHDPFQTIHMGVTAENVAKKYDISRDAQDALA
CCCCEECCCHHHHHHCCCHHHHHHHHHHHCCCHHHHEECCCHHHHHHHHCCCCCHHHHHH
LESHHRAAKAIEAGYFKEQILPITIASKKGDTVFDTDEHVRLNATADDFSKLKPVFAKEN
HHHHHHHHHHHHHHHHHHCEEEEEEECCCCCEEECCCCCEEEECCHHHHHHCCCCEECCC
GTVTAGNASGINDAAAAVVLMERSVAEKRGMKPLGRLVAYAHAGVDPNYMGIGPVPATQK
CEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHH
VLERAGLTVADLDVIEANEAFAAQACAVSKELGLDPAKVNPNGSGISLGHPIGATGALIT
HHHHCCCEEEEEEEEECCHHHHHHHHHHHHHHCCCCCEECCCCCCEECCCCCCCCHHHHH
VKALYELQRIGGRYALVTMCIGGGQGIAAIFERI
HHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]