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The map label for this gene is pcm

Identifier: 186475725

GI number: 186475725

Start: 1072202

End: 1073185

Strand: Direct

Name: pcm

Synonym: Bphy_0960

Alternate gene names: 186475725

Gene position: 1072202-1073185 (Clockwise)

Preceding gene: 186475724

Following gene: 186475726

Centisome position: 30.82

GC content: 66.06

Gene sequence:

>984_bases
ATGACCGGCGAGCGCGCAAAGCGCTTTCCGCTGGGACTCGAAGACCTGGTGCGCGAGCCACGCCGGGCCGATGCGCGCGA
TGCGAAAACCGGCAAGCCGGCCGTGCCGAAGCCGGTCACATCGAAACCGGCCGGTTCGTCCACGCGGGCGCCTGTCGTGG
TCACACCCGTGGTCAAACCGGTCGTCAAAAGCGCCGTTACAAAAAACGCGTCCGGAATCGTCAATACGAAAAGCGCATCC
ACGATCCGCAACGACGGCGCGCGCAACCAGTTGCCGCGTCCCGCGACGGCAGCGTTCGAACGCACGGGCGCGCCGAATGT
CGCGTTGACGAGCGCGGTCACGCTGACGTCCGAACGCGTGCGCGAGCGGATGGTCGAAAGGCTGCGTGCGAACGGCATCA
CGGACCCGCGCGTGCTCGACGCGATGGCGATGGTGCCGCGCCACATGTTCGTGGATCCCGGCCTCGCTACGCAGGCGTAC
GAAGACGCGGCGCTGCCCATCGGCCATCATCAGACCATCTCGAAGCCTTCTGTGGTGGCGCGGATGATCGAGCTGGCGGC
GCAGGGCCGTACGCTCAACAACGTGCTCGAAATCGGCACGGGCTGCGGCTATCAGGCTGCGGTACTGAGCCAGGTCGCGC
GCGACGTGTATTCGATTGAACGCATCAAACCGCTTTACGAACGCGCAAAGACGAACCTGCGCCCGCTGCGCATTCCGAAC
ATCCGTCTGCACTATGGCGACGGACGCATCGGGTTGCCCGCGGCTGCGCCGTTCGATGCGATCGTGATCGCGGCAGCCGG
ACTCGATGTGCCGCAGGCGCTGCGCGAGCAGCTCGCTATCGGCGCGCGGCTCGTGGCGCCCGTCGGATCGCAGGACGGAC
AGTCGCAGGTGCTCACGCTCGTCGAGCGCACGGGGCCCGCGCAGTGGCGCGAATCGCGGCTTGATCGCGTTTTCTTTGTC
CCCTTAAAATCCGGAGTGATTTGA

Upstream 100 bases:

>100_bases
CGAACGGTTTCGTGTCGATCACGCCGCTGCAACTTGACTTGACGGATACGGCGATGCTGCCCACGGCGTGCGACTGGGTG
CGCGCCGGGAGCGGGACTTC

Downstream 100 bases:

>100_bases
ACCCGATGAGTATGTTTCGCGCGATGCAAAGAACCAGCCTGAATGTCCCGTTGTCCGTCGCTCAGCGCAGCGTTTGCGTG
GCCATGCTGTCCGTCCTGGC

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MTGERAKRFPLGLEDLVREPRRADARDAKTGKPAVPKPVTSKPAGSSTRAPVVVTPVVKPVVKSAVTKNASGIVNTKSAS
TIRNDGARNQLPRPATAAFERTGAPNVALTSAVTLTSERVRERMVERLRANGITDPRVLDAMAMVPRHMFVDPGLATQAY
EDAALPIGHHQTISKPSVVARMIELAAQGRTLNNVLEIGTGCGYQAAVLSQVARDVYSIERIKPLYERAKTNLRPLRIPN
IRLHYGDGRIGLPAAAPFDAIVIAAAGLDVPQALREQLAIGARLVAPVGSQDGQSQVLTLVERTGPAQWRESRLDRVFFV
PLKSGVI

Sequences:

>Translated_327_residues
MTGERAKRFPLGLEDLVREPRRADARDAKTGKPAVPKPVTSKPAGSSTRAPVVVTPVVKPVVKSAVTKNASGIVNTKSAS
TIRNDGARNQLPRPATAAFERTGAPNVALTSAVTLTSERVRERMVERLRANGITDPRVLDAMAMVPRHMFVDPGLATQAY
EDAALPIGHHQTISKPSVVARMIELAAQGRTLNNVLEIGTGCGYQAAVLSQVARDVYSIERIKPLYERAKTNLRPLRIPN
IRLHYGDGRIGLPAAAPFDAIVIAAAGLDVPQALREQLAIGARLVAPVGSQDGQSQVLTLVERTGPAQWRESRLDRVFFV
PLKSGVI
>Mature_326_residues
TGERAKRFPLGLEDLVREPRRADARDAKTGKPAVPKPVTSKPAGSSTRAPVVVTPVVKPVVKSAVTKNASGIVNTKSAST
IRNDGARNQLPRPATAAFERTGAPNVALTSAVTLTSERVRERMVERLRANGITDPRVLDAMAMVPRHMFVDPGLATQAYE
DAALPIGHHQTISKPSVVARMIELAAQGRTLNNVLEIGTGCGYQAAVLSQVARDVYSIERIKPLYERAKTNLRPLRIPNI
RLHYGDGRIGLPAAAPFDAIVIAAAGLDVPQALREQLAIGARLVAPVGSQDGQSQVLTLVERTGPAQWRESRLDRVFFVP
LKSGVI

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=213, Percent_Identity=32.3943661971831, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1789100, Length=214, Percent_Identity=44.8598130841121, Blast_Score=155, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI71983477, Length=220, Percent_Identity=31.8181818181818, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI193207222, Length=219, Percent_Identity=31.5068493150685, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI17981723, Length=216, Percent_Identity=31.9444444444444, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_BURP8 (B2JG67)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001857195.1
- ProteinModelPortal:   B2JG67
- SMR:   B2JG67
- GeneID:   6242458
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_0960
- HOGENOM:   HBG699907
- OMA:   STIRNDG
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 35123; Mature: 34992

Theoretical pI: Translated: 11.15; Mature: 11.15

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 174-174

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGERAKRFPLGLEDLVREPRRADARDAKTGKPAVPKPVTSKPAGSSTRAPVVVTPVVKP
CCCCCCCCCCCCHHHHHCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECHHHHH
VVKSAVTKNASGIVNTKSASTIRNDGARNQLPRPATAAFERTGAPNVALTSAVTLTSERV
HHHHHHHCCCCCCEECCCHHHHHCCCCCCCCCCCHHHHHHHCCCCCEEHHHHHHHHHHHH
RERMVERLRANGITDPRVLDAMAMVPRHMFVDPGLATQAYEDAALPIGHHQTISKPSVVA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHH
RMIELAAQGRTLNNVLEIGTGCGYQAAVLSQVARDVYSIERIKPLYERAKTNLRPLRIPN
HHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCC
IRLHYGDGRIGLPAAAPFDAIVIAAAGLDVPQALREQLAIGARLVAPVGSQDGQSQVLTL
EEEEECCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCHHEECCCCCCCCHHHHHHH
VERTGPAQWRESRLDRVFFVPLKSGVI
HHHCCCHHHHHHHCCEEEEEECCCCCC
>Mature Secondary Structure 
TGERAKRFPLGLEDLVREPRRADARDAKTGKPAVPKPVTSKPAGSSTRAPVVVTPVVKP
CCCCCCCCCCCHHHHHCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECHHHHH
VVKSAVTKNASGIVNTKSASTIRNDGARNQLPRPATAAFERTGAPNVALTSAVTLTSERV
HHHHHHHCCCCCCEECCCHHHHHCCCCCCCCCCCHHHHHHHCCCCCEEHHHHHHHHHHHH
RERMVERLRANGITDPRVLDAMAMVPRHMFVDPGLATQAYEDAALPIGHHQTISKPSVVA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHH
RMIELAAQGRTLNNVLEIGTGCGYQAAVLSQVARDVYSIERIKPLYERAKTNLRPLRIPN
HHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCC
IRLHYGDGRIGLPAAAPFDAIVIAAAGLDVPQALREQLAIGARLVAPVGSQDGQSQVLTL
EEEEECCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCHHEECCCCCCCCHHHHHHH
VERTGPAQWRESRLDRVFFVPLKSGVI
HHHCCCHHHHHHHCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA