| Definition | Burkholderia phymatum STM815 chromosome chromosome 1, complete sequence. |
|---|---|
| Accession | NC_010622 |
| Length | 3,479,187 |
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The map label for this gene is clpB [H]
Identifier: 186475703
GI number: 186475703
Start: 1041221
End: 1043818
Strand: Direct
Name: clpB [H]
Synonym: Bphy_0938
Alternate gene names: 186475703
Gene position: 1041221-1043818 (Clockwise)
Preceding gene: 186475702
Following gene: 186475708
Centisome position: 29.93
GC content: 62.28
Gene sequence:
>2598_bases ATGAGAATCGACAAACTCACCACTAAATTCCAGGAAGCGTTGGCCGACGCGCAGAGCCTGGCCGTCGGTCATGACAATCA GTACATCGAGCCTGTGCATCTGCTCGCCGCTCTTGTCGCGCAGCAGGACGGCTCCGCGCGTTCGCTGCTGGCACGCGCCG GCGTTCATGTGCAGGCGCTGCAAACCGCGCTGAACGACGCCATCACGCGGCTGCCGCAAGTGCAGGGCACGGACGGCAAC GTGCAGATCGGGCGCGATCTCACGGGTCTGTTGAACCAGGCCGACAAGGAAGCACAGAAGCTCAACGACACCTACATCGC GAGCGAAATGTTTTTGCTCGCTGTCGCTGACGACAAGGGCGAAGCCGGGCGCCTCGCGCGCGAGCACGGCTTGTCGCGCA AGTCGCTCGAAGCGGCCATCGTCGCCGTGCGCGGCGGTGCGCAGGTGCACAGCCAGGACGCTGAAAGCCAGCGCGAGGCG CTGAAGAAATACACGATCGATCTGACCGAGCGCGCTCGGGCGGGCAAGCTCGACCCGGTGATCGGGCGCGACGACGAAAT CCGCCGTTCGATCCAGATCCTGCAGCGCCGCACCAAGAACAACCCGGTGCTGATCGGCGAGCCGGGCGTCGGCAAGACGG CGATTGTCGAAGGTCTCGCGCAGCGCATCGTCAACGGCGAAGTGCCGGAAACGCTAAAGGGCAAGCGTGTGTTGTCGCTC GACATGGCGGCGCTGCTCGCCGGCGCGAAGTATCGCGGCGAGTTCGAAGAACGGCTGAAGGCCGTGCTGAACGACATCGC GAAGGACGAAGGGCAGACCATCGTCTTCATCGACGAAATTCACACAATGGTCGGTGCCGGCAAGGCCGAAGGCGCGATGG ACGCGGGCAACATGCTGAAGCCGGCGCTGTCGCGCGGCGAACTGCATTGCGTCGGCGCGACCACGCTGGACGAATATCGC AAGTACATCGAAAAGGATGCCGCGCTGGAGCGCCGTTTTCAGAAAGTGCTCGTCGACGAACCCAGCGTGGAAGCAACCAT CGCGATCCTGCGCGGCTTGCAGGAACGTTATGAACTGCACCACGGCGTCGACATTACCGACCCCGCCATCGTCGCCGCGG CGGAACTGTCGCATCGCTACATCACCGACCGTTTCCTGCCGGATAAGGCGATTGACCTCATCGACGAAGCCGCGTCGAAG ATCAAGATGGAAATCGACTCGAAGCCCGAGGAGATGGACCGGCTCGACCGTCGGCGCATCCAGTTGAAGATCGAGCGCGA AGCCGTCAAGAAGGAGAAGGACGAAGCGTCACAGAAGCGTCTACAACTGATCGAGGAAGAAATCCAGCGGCTAGACCGCG AATATTCGGACCTGGAAGAAATCTGGACGGCGGAAAAAGCCGCCGTGCAGGGCAGTGCGCAGCTGAAGGAAGAGATCGAG AAGACGCGCGCGGAAATTACGCGTCTGCAACGCGAAGGCAAGCTCGAAAAGGTCGCCGAACTGCAATACGGCAAGCTGCC GCAACTCGAGGCGCAACTGAAGGCGGTCACGAAGGCCGAGGCGGAAGAGCAGAATAATCCGACGCGTCCGCGTCTGTTGC GCACGCAGGTCGGCGCCGAGGAAATCGCCGAAGTCGTGTCGCGCGCAACGGGCATTCCCGTGTCGCGGATGATGCAGGGC GAGCGTGAGAAGCTGCTGCAGATCGAAGAGAAGTTGCATCAGCGCGTGGTCGGCCAGGATGAGGCGATCAGTGCGGTGGC CGATGCGATCCGCCGCTCGCGTGCCGGCCTGTCGGATCCGAATCGTCCGTATGGCTCGTTCCTGTTCCTGGGGCCGACGG GCGTCGGCAAGACGGAACTGTGCAAGGCGCTCGCGGCATTTCTCTTCGACGCGGAAGACCATCTCATCCGCATCGACATG AGCGAGTTCATGGAGAAGCACAGCGTCGCGCGTCTGATCGGCGCGCCGCCGGGCTATGTGGGTTACGAGGAAGGCGGCTA TCTGACGGAAGCGGTGCGTCGCAAGCCGTACAGCGTGATCCTGCTCGACGAAATCGAGAAGGCGCATCCTGACGTCTTCA ATGTGCTGCTGCAGGTGCTCGACGACGGCCGCATGACGGACGGCCAGGGCCGCACGGTGGACTTCAAGAACACGGTGATC GTGATGACATCGAACCTCGGCTCGCACGTCATTCAGTCGATGGTCGGCGAGCCGCAGGAAGCCGTGAAGGACGCAGTCTG GGAAGAAGTGAAGCTGCATTTCCGGCCGGAATTCCTGAACCGGATCGACGATGTGGTCGTGTTTCATGCGCTCGATCGGG CGAACATCCAGTCGATTGCGAAGATTCAGCTGGAACGGGTGCACGAGCGGCTTGCGAAGCTCGACATGCAGCTGGTCGTA TCGGAGGCCGCGCTGGAGCTGATCGGCAAGGTCGGCTACGACCCGTTGTTCGGCGCGCGGCCATTGAAGCGTGCAATCCA GCAGGAGATCGAGAACCCGGTCGCGAAGCTGATTCTGGCCGGCAAGTTCGGGCCGAAGGACGTGATTCCCGTCGAAGTCG AAAACGGCAAGCTGGTGTTCGAGCGGGTGGTGCACTGA
Upstream 100 bases:
>100_bases TGCGGACCGCGTGCACATCCTCTCTTCTTCGCGCAAAAAAGCGCTTGAAACCCAAATAAAACGCCTCATTTTGGTGGTAA TCGAAATCGGAGGTCGCTTG
Downstream 100 bases:
>100_bases TCATTCACGGGAGCGGAGCGTCGAAAGCAGACGCTTCGTTCCGGATGAAAAGCAAAAGGGCAACGAAGCGATTCGTTGCC CTTTTTTTCACGCACGGCGT
Product: ATP-dependent chaperone ClpB
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 865; Mature: 865
Protein sequence:
>865_residues MRIDKLTTKFQEALADAQSLAVGHDNQYIEPVHLLAALVAQQDGSARSLLARAGVHVQALQTALNDAITRLPQVQGTDGN VQIGRDLTGLLNQADKEAQKLNDTYIASEMFLLAVADDKGEAGRLAREHGLSRKSLEAAIVAVRGGAQVHSQDAESQREA LKKYTIDLTERARAGKLDPVIGRDDEIRRSIQILQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPETLKGKRVLSL DMAALLAGAKYRGEFEERLKAVLNDIAKDEGQTIVFIDEIHTMVGAGKAEGAMDAGNMLKPALSRGELHCVGATTLDEYR KYIEKDAALERRFQKVLVDEPSVEATIAILRGLQERYELHHGVDITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAASK IKMEIDSKPEEMDRLDRRRIQLKIEREAVKKEKDEASQKRLQLIEEEIQRLDREYSDLEEIWTAEKAAVQGSAQLKEEIE KTRAEITRLQREGKLEKVAELQYGKLPQLEAQLKAVTKAEAEEQNNPTRPRLLRTQVGAEEIAEVVSRATGIPVSRMMQG EREKLLQIEEKLHQRVVGQDEAISAVADAIRRSRAGLSDPNRPYGSFLFLGPTGVGKTELCKALAAFLFDAEDHLIRIDM SEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEIEKAHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVI VMTSNLGSHVIQSMVGEPQEAVKDAVWEEVKLHFRPEFLNRIDDVVVFHALDRANIQSIAKIQLERVHERLAKLDMQLVV SEAALELIGKVGYDPLFGARPLKRAIQQEIENPVAKLILAGKFGPKDVIPVEVENGKLVFERVVH
Sequences:
>Translated_865_residues MRIDKLTTKFQEALADAQSLAVGHDNQYIEPVHLLAALVAQQDGSARSLLARAGVHVQALQTALNDAITRLPQVQGTDGN VQIGRDLTGLLNQADKEAQKLNDTYIASEMFLLAVADDKGEAGRLAREHGLSRKSLEAAIVAVRGGAQVHSQDAESQREA LKKYTIDLTERARAGKLDPVIGRDDEIRRSIQILQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPETLKGKRVLSL DMAALLAGAKYRGEFEERLKAVLNDIAKDEGQTIVFIDEIHTMVGAGKAEGAMDAGNMLKPALSRGELHCVGATTLDEYR KYIEKDAALERRFQKVLVDEPSVEATIAILRGLQERYELHHGVDITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAASK IKMEIDSKPEEMDRLDRRRIQLKIEREAVKKEKDEASQKRLQLIEEEIQRLDREYSDLEEIWTAEKAAVQGSAQLKEEIE KTRAEITRLQREGKLEKVAELQYGKLPQLEAQLKAVTKAEAEEQNNPTRPRLLRTQVGAEEIAEVVSRATGIPVSRMMQG EREKLLQIEEKLHQRVVGQDEAISAVADAIRRSRAGLSDPNRPYGSFLFLGPTGVGKTELCKALAAFLFDAEDHLIRIDM SEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEIEKAHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVI VMTSNLGSHVIQSMVGEPQEAVKDAVWEEVKLHFRPEFLNRIDDVVVFHALDRANIQSIAKIQLERVHERLAKLDMQLVV SEAALELIGKVGYDPLFGARPLKRAIQQEIENPVAKLILAGKFGPKDVIPVEVENGKLVFERVVH >Mature_865_residues MRIDKLTTKFQEALADAQSLAVGHDNQYIEPVHLLAALVAQQDGSARSLLARAGVHVQALQTALNDAITRLPQVQGTDGN VQIGRDLTGLLNQADKEAQKLNDTYIASEMFLLAVADDKGEAGRLAREHGLSRKSLEAAIVAVRGGAQVHSQDAESQREA LKKYTIDLTERARAGKLDPVIGRDDEIRRSIQILQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPETLKGKRVLSL DMAALLAGAKYRGEFEERLKAVLNDIAKDEGQTIVFIDEIHTMVGAGKAEGAMDAGNMLKPALSRGELHCVGATTLDEYR KYIEKDAALERRFQKVLVDEPSVEATIAILRGLQERYELHHGVDITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAASK IKMEIDSKPEEMDRLDRRRIQLKIEREAVKKEKDEASQKRLQLIEEEIQRLDREYSDLEEIWTAEKAAVQGSAQLKEEIE KTRAEITRLQREGKLEKVAELQYGKLPQLEAQLKAVTKAEAEEQNNPTRPRLLRTQVGAEEIAEVVSRATGIPVSRMMQG EREKLLQIEEKLHQRVVGQDEAISAVADAIRRSRAGLSDPNRPYGSFLFLGPTGVGKTELCKALAAFLFDAEDHLIRIDM SEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEIEKAHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVI VMTSNLGSHVIQSMVGEPQEAVKDAVWEEVKLHFRPEFLNRIDDVVVFHALDRANIQSIAKIQLERVHERLAKLDMQLVV SEAALELIGKVGYDPLFGARPLKRAIQQEIENPVAKLILAGKFGPKDVIPVEVENGKLVFERVVH
Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase
COG id: COG0542
COG function: function code O; ATPases with chaperone activity, ATP-binding subunit
Gene ontology:
Cell location: Cytoplasm (Probable) [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the clpA/clpB family [H]
Homologues:
Organism=Homo sapiens, GI13540606, Length=298, Percent_Identity=36.241610738255, Blast_Score=196, Evalue=6e-50, Organism=Escherichia coli, GI1788943, Length=860, Percent_Identity=67.2093023255814, Blast_Score=1179, Evalue=0.0, Organism=Escherichia coli, GI1787109, Length=401, Percent_Identity=41.1471321695761, Blast_Score=282, Evalue=8e-77, Organism=Saccharomyces cerevisiae, GI6320464, Length=712, Percent_Identity=52.3876404494382, Blast_Score=753, Evalue=0.0, Organism=Saccharomyces cerevisiae, GI6323002, Length=865, Percent_Identity=42.7745664739884, Blast_Score=692, Evalue=0.0,
Paralogues:
None
Copy number: 560 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003593 - InterPro: IPR013093 - InterPro: IPR003959 - InterPro: IPR018368 - InterPro: IPR017730 - InterPro: IPR001270 - InterPro: IPR019489 - InterPro: IPR004176 - InterPro: IPR023150 [H]
Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]
EC number: NA
Molecular weight: Translated: 96183; Mature: 96183
Theoretical pI: Translated: 5.57; Mature: 5.57
Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.2 %Cys (Translated Protein) 1.8 %Met (Translated Protein) 2.1 %Cys+Met (Translated Protein) 0.2 %Cys (Mature Protein) 1.8 %Met (Mature Protein) 2.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MRIDKLTTKFQEALADAQSLAVGHDNQYIEPVHLLAALVAQQDGSARSLLARAGVHVQAL CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCHHHHH QTALNDAITRLPQVQGTDGNVQIGRDLTGLLNQADKEAQKLNDTYIASEMFLLAVADDKG HHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCC EAGRLAREHGLSRKSLEAAIVAVRGGAQVHSQDAESQREALKKYTIDLTERARAGKLDPV CHHHHHHHHCCCHHHHHHHHEEEECCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC IGRDDEIRRSIQILQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPETLKGKRVLSL CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCHHCCCEEHHH DMAALLAGAKYRGEFEERLKAVLNDIAKDEGQTIVFIDEIHTMVGAGKAEGAMDAGNMLK HHHHHHHCCHHCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCCCCCCCCCCHHHH PALSRGELHCVGATTLDEYRKYIEKDAALERRFQKVLVDEPSVEATIAILRGLQERYELH HHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH HGVDITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAASKIKMEIDSKPEEMDRLDRRRI CCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEHHCCCCHHHHHHHHHHHH QLKIEREAVKKEKDEASQKRLQLIEEEIQRLDREYSDLEEIWTAEKAAVQGSAQLKEEIE HHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH KTRAEITRLQREGKLEKVAELQYGKLPQLEAQLKAVTKAEAEEQNNPTRPRLLRTQVGAE HHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHH EIAEVVSRATGIPVSRMMQGEREKLLQIEEKLHQRVVGQDEAISAVADAIRRSRAGLSDP HHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC NRPYGSFLFLGPTGVGKTELCKALAAFLFDAEDHLIRIDMSEFMEKHSVARLIGAPPGYV CCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHCCCCCCC GYEEGGYLTEAVRRKPYSVILLDEIEKAHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVI CCCCCCHHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEE VMTSNLGSHVIQSMVGEPQEAVKDAVWEEVKLHFRPEFLNRIDDVVVFHALDRANIQSIA EEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHH KIQLERVHERLAKLDMQLVVSEAALELIGKVGYDPLFGARPLKRAIQQEIENPVAKLILA HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH GKFGPKDVIPVEVENGKLVFERVVH CCCCCCCCEEEEECCCCCHHHHHCC >Mature Secondary Structure MRIDKLTTKFQEALADAQSLAVGHDNQYIEPVHLLAALVAQQDGSARSLLARAGVHVQAL CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCHHHHH QTALNDAITRLPQVQGTDGNVQIGRDLTGLLNQADKEAQKLNDTYIASEMFLLAVADDKG HHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCC EAGRLAREHGLSRKSLEAAIVAVRGGAQVHSQDAESQREALKKYTIDLTERARAGKLDPV CHHHHHHHHCCCHHHHHHHHEEEECCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC IGRDDEIRRSIQILQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPETLKGKRVLSL CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCHHCCCEEHHH DMAALLAGAKYRGEFEERLKAVLNDIAKDEGQTIVFIDEIHTMVGAGKAEGAMDAGNMLK HHHHHHHCCHHCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCCCCCCCCCCHHHH PALSRGELHCVGATTLDEYRKYIEKDAALERRFQKVLVDEPSVEATIAILRGLQERYELH HHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH HGVDITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAASKIKMEIDSKPEEMDRLDRRRI CCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEHHCCCCHHHHHHHHHHHH QLKIEREAVKKEKDEASQKRLQLIEEEIQRLDREYSDLEEIWTAEKAAVQGSAQLKEEIE HHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH KTRAEITRLQREGKLEKVAELQYGKLPQLEAQLKAVTKAEAEEQNNPTRPRLLRTQVGAE HHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHH EIAEVVSRATGIPVSRMMQGEREKLLQIEEKLHQRVVGQDEAISAVADAIRRSRAGLSDP HHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC NRPYGSFLFLGPTGVGKTELCKALAAFLFDAEDHLIRIDMSEFMEKHSVARLIGAPPGYV CCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHCCCCCCC GYEEGGYLTEAVRRKPYSVILLDEIEKAHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVI CCCCCCHHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEE VMTSNLGSHVIQSMVGEPQEAVKDAVWEEVKLHFRPEFLNRIDDVVVFHALDRANIQSIA EEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHH KIQLERVHERLAKLDMQLVVSEAALELIGKVGYDPLFGARPLKRAIQQEIENPVAKLILA HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH GKFGPKDVIPVEVENGKLVFERVVH CCCCCCCCEEEEECCCCCHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 11823852 [H]