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The map label for this gene is lepA [H]

Identifier: 186475604

GI number: 186475604

Start: 937214

End: 939007

Strand: Direct

Name: lepA [H]

Synonym: Bphy_0839

Alternate gene names: 186475604

Gene position: 937214-939007 (Clockwise)

Preceding gene: 186475603

Following gene: 186475605

Centisome position: 26.94

GC content: 59.53

Gene sequence:

>1794_bases
ATGGATCATATTCGTAACTTTTCGATCATTGCGCACATCGACCACGGCAAGTCGACGCTCGCCGATCGCATCATCCAGAT
TTGCGGCGGTCTCTCGGACCGCGAGATGGAAGCACAAGTTCTCGACTCGATGGATCTCGAGCGCGAGCGCGGCATCACCA
TCAAGGCTCAGACGGCTGCACTGTCGTATCGCGCGCGCGATGGCAAGCTGTACAACCTGAACATGATCGACACGCCCGGG
CACGTCGACTTCTCTTATGAAGTCAGCCGTTCGCTGTCCGCGTGTGAAGGCGCGCTGCTGGTCGTCGACGCGAGTCAGGG
CGTCGAGGCGCAGACTGTCGCGAACTGCTACACGGCGATCGAACTAGGCGTCGAGGTCGTGCCCGTGCTCAACAAGATCG
ACCTGCCTGCAGCCAACCCGGAAAACGCGATCGCCGAAATCGAGGACGTAATCGGCATCGACGCAGCTGACGCGACGCCT
TGCAGCGCGAAGACGGGCATGGGCGTCGAAGACGTGCTCGAAGCGCTGATCGCCAAGGTGCCGCCGCCCAAGGGCGACGC
GGGCCAGCCGTTGCAGGCATTGATCATCGACTCGTGGTTCGACAACTACGTCGGCGTCGTGATGCTGGTGCGCATCGTCA
ACGGCACGCTGCGTCCCAAGGACCGCATTCGCTTGATGGCGACGGGCGCCGAGTATCCCGTCGAGCACGTCGGCGTGTTC
ACACCCAAGTCGAAGAATCTCGAGTCGCTGTCGGCGGGGCAGGTGGGCTTCATCATCGCGGGTATCAAGGAACTGACGGC
TGCGAAGGTCGGCGATACCGTGACCACCGTGAAGAACGCGGCGCCGGAACCATTGCCCGGCTTCAAGGAAGTGAAGCCAC
AGGTGTTCGCTGGTCTCTATCCCGTCGAAGCGAACCAGTACGACGCCCTGCGCGAATCGCTGGAAAAGCTCAAGCTCAAC
GATGCATCTCTGATGTACGAGCCGGAAGTGTCCCAGGCACTCGGCTTCGGCTTCCGCTGCGGCTTCCTCGGGCTGTTGCA
TATGGAAATTGTCCAGGAACGTCTCGAACGCGAGTTCGATATGGACCTGATCACGACCGCGCCGACCGTGGTCTACGAGG
TCGTTCAGCGCGACGGTACGACGATCATGGTCGAGAACCCGGCCAAGATGCCGGACCCGTCGAAGATCGAAGAAGTGCGC
GAGCCGATCGTCACTGTGAATCTGTACATGCCGCAAGACTATGTCGGCTCGGTGATCACGCTGTGTACGCAAAAGCGTGG
CGTGCAAATCAACATGCAATATCACGGCCGCCAGGTGCAGTTGACGTACGAGATCCCGATGGGCGAAATCGTGCTCGACT
TCTTCGACCGTCTGAAGTCGGTGTCGCGCGGCTATGCGTCGATGGATTACGAGTTCAAGGAATATCGCGCGTCGGACGTG
GTGAAGGTCGACATGCTGATCAACGGCGATAAGGTCGACGCGCTATCCGTCATCGTGCACCGTTCGCAGAGCCAGTATCG
CGGCCGTGAAGTCGCGTCGAAGATGCGCGAAATCATTCCGCGACAGATGTACGACGTCGCGATCCAGGCCACCATCGGCG
CACACATCATCGCGCGCGAGAACATCAAGGCACTGCGCAAGAACGTTCTTGCCAAGTGTTATGGCGGCGACATCACACGT
AAGAAGAAGCTGCTGGAAAAACAGAAGGAAGGCAAGAAGCGGATGAAGCAAGTCGGCTCCGTCGAGATCCCGCAAGAGGC
TTTCCTCGCGATTCTGCGCGTCGAAGACAAATAA

Upstream 100 bases:

>100_bases
GGCGCCCCCCTTTTCGGCTAAAATAGATAAGTTTTTCACTTACTTACAAGGCGTGCTCCGCAATCGTCCGAGCGCGCCTT
TTTCGCTTGATCGGCACTGA

Downstream 100 bases:

>100_bases
GACTAAAGAACTGGAACCCTATGAATTTTGCGCTGATTCTTTTTGTGCTCGTGATTCTCACGGGCGTGGCATGGGTCGCG
GACAAACTGGTTTTCCTGCC

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 597; Mature: 597

Protein sequence:

>597_residues
MDHIRNFSIIAHIDHGKSTLADRIIQICGGLSDREMEAQVLDSMDLERERGITIKAQTAALSYRARDGKLYNLNMIDTPG
HVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTAIELGVEVVPVLNKIDLPAANPENAIAEIEDVIGIDAADATP
CSAKTGMGVEDVLEALIAKVPPPKGDAGQPLQALIIDSWFDNYVGVVMLVRIVNGTLRPKDRIRLMATGAEYPVEHVGVF
TPKSKNLESLSAGQVGFIIAGIKELTAAKVGDTVTTVKNAAPEPLPGFKEVKPQVFAGLYPVEANQYDALRESLEKLKLN
DASLMYEPEVSQALGFGFRCGFLGLLHMEIVQERLEREFDMDLITTAPTVVYEVVQRDGTTIMVENPAKMPDPSKIEEVR
EPIVTVNLYMPQDYVGSVITLCTQKRGVQINMQYHGRQVQLTYEIPMGEIVLDFFDRLKSVSRGYASMDYEFKEYRASDV
VKVDMLINGDKVDALSVIVHRSQSQYRGREVASKMREIIPRQMYDVAIQATIGAHIIARENIKALRKNVLAKCYGGDITR
KKKLLEKQKEGKKRMKQVGSVEIPQEAFLAILRVEDK

Sequences:

>Translated_597_residues
MDHIRNFSIIAHIDHGKSTLADRIIQICGGLSDREMEAQVLDSMDLERERGITIKAQTAALSYRARDGKLYNLNMIDTPG
HVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTAIELGVEVVPVLNKIDLPAANPENAIAEIEDVIGIDAADATP
CSAKTGMGVEDVLEALIAKVPPPKGDAGQPLQALIIDSWFDNYVGVVMLVRIVNGTLRPKDRIRLMATGAEYPVEHVGVF
TPKSKNLESLSAGQVGFIIAGIKELTAAKVGDTVTTVKNAAPEPLPGFKEVKPQVFAGLYPVEANQYDALRESLEKLKLN
DASLMYEPEVSQALGFGFRCGFLGLLHMEIVQERLEREFDMDLITTAPTVVYEVVQRDGTTIMVENPAKMPDPSKIEEVR
EPIVTVNLYMPQDYVGSVITLCTQKRGVQINMQYHGRQVQLTYEIPMGEIVLDFFDRLKSVSRGYASMDYEFKEYRASDV
VKVDMLINGDKVDALSVIVHRSQSQYRGREVASKMREIIPRQMYDVAIQATIGAHIIARENIKALRKNVLAKCYGGDITR
KKKLLEKQKEGKKRMKQVGSVEIPQEAFLAILRVEDK
>Mature_597_residues
MDHIRNFSIIAHIDHGKSTLADRIIQICGGLSDREMEAQVLDSMDLERERGITIKAQTAALSYRARDGKLYNLNMIDTPG
HVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTAIELGVEVVPVLNKIDLPAANPENAIAEIEDVIGIDAADATP
CSAKTGMGVEDVLEALIAKVPPPKGDAGQPLQALIIDSWFDNYVGVVMLVRIVNGTLRPKDRIRLMATGAEYPVEHVGVF
TPKSKNLESLSAGQVGFIIAGIKELTAAKVGDTVTTVKNAAPEPLPGFKEVKPQVFAGLYPVEANQYDALRESLEKLKLN
DASLMYEPEVSQALGFGFRCGFLGLLHMEIVQERLEREFDMDLITTAPTVVYEVVQRDGTTIMVENPAKMPDPSKIEEVR
EPIVTVNLYMPQDYVGSVITLCTQKRGVQINMQYHGRQVQLTYEIPMGEIVLDFFDRLKSVSRGYASMDYEFKEYRASDV
VKVDMLINGDKVDALSVIVHRSQSQYRGREVASKMREIIPRQMYDVAIQATIGAHIIARENIKALRKNVLAKCYGGDITR
KKKLLEKQKEGKKRMKQVGSVEIPQEAFLAILRVEDK

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=604, Percent_Identity=49.1721854304636, Blast_Score=614, Evalue=1e-176,
Organism=Homo sapiens, GI18390331, Length=161, Percent_Identity=39.1304347826087, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI94966754, Length=133, Percent_Identity=45.1127819548872, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI25306283, Length=180, Percent_Identity=42.2222222222222, Blast_Score=104, Evalue=3e-22,
Organism=Homo sapiens, GI19923640, Length=180, Percent_Identity=42.2222222222222, Blast_Score=104, Evalue=3e-22,
Organism=Homo sapiens, GI25306287, Length=180, Percent_Identity=42.2222222222222, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI4503483, Length=144, Percent_Identity=41.6666666666667, Blast_Score=103, Evalue=6e-22,
Organism=Homo sapiens, GI310132016, Length=110, Percent_Identity=42.7272727272727, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI310110807, Length=110, Percent_Identity=42.7272727272727, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI310123363, Length=110, Percent_Identity=42.7272727272727, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI217272894, Length=133, Percent_Identity=34.5864661654135, Blast_Score=78, Evalue=3e-14,
Organism=Homo sapiens, GI217272892, Length=133, Percent_Identity=34.5864661654135, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI53729339, Length=218, Percent_Identity=29.8165137614679, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI53729337, Length=218, Percent_Identity=29.8165137614679, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI4503471, Length=346, Percent_Identity=25.1445086705202, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788922, Length=594, Percent_Identity=69.6969696969697, Blast_Score=852, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=510, Percent_Identity=29.0196078431373, Blast_Score=172, Evalue=8e-44,
Organism=Escherichia coli, GI1789738, Length=178, Percent_Identity=34.8314606741573, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI1790835, Length=171, Percent_Identity=30.4093567251462, Blast_Score=83, Evalue=6e-17,
Organism=Escherichia coli, GI1789559, Length=289, Percent_Identity=28.719723183391, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17557151, Length=613, Percent_Identity=39.3148450244698, Blast_Score=466, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI17556745, Length=464, Percent_Identity=24.7844827586207, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17533571, Length=162, Percent_Identity=36.4197530864198, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17506493, Length=156, Percent_Identity=38.4615384615385, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI71988819, Length=134, Percent_Identity=38.0597014925373, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71988811, Length=134, Percent_Identity=38.0597014925373, Blast_Score=91, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17552882, Length=133, Percent_Identity=34.5864661654135, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI32566303, Length=243, Percent_Identity=29.2181069958848, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71994658, Length=220, Percent_Identity=29.0909090909091, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=602, Percent_Identity=47.0099667774086, Blast_Score=562, Evalue=1e-161,
Organism=Saccharomyces cerevisiae, GI6323098, Length=199, Percent_Identity=36.1809045226131, Blast_Score=113, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6324707, Length=144, Percent_Identity=41.6666666666667, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6320593, Length=144, Percent_Identity=41.6666666666667, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322359, Length=115, Percent_Identity=41.7391304347826, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324166, Length=144, Percent_Identity=38.8888888888889, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6325337, Length=156, Percent_Identity=31.4102564102564, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6319594, Length=156, Percent_Identity=31.4102564102564, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324761, Length=282, Percent_Identity=25.531914893617, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI78706572, Length=602, Percent_Identity=45.514950166113, Blast_Score=542, Evalue=1e-154,
Organism=Drosophila melanogaster, GI24582462, Length=162, Percent_Identity=38.2716049382716, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24585709, Length=148, Percent_Identity=37.1621621621622, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24585711, Length=148, Percent_Identity=37.1621621621622, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24585713, Length=148, Percent_Identity=37.1621621621622, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI28574573, Length=137, Percent_Identity=43.0656934306569, Blast_Score=95, Evalue=2e-19,
Organism=Drosophila melanogaster, GI221458488, Length=149, Percent_Identity=37.5838926174497, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21357743, Length=133, Percent_Identity=34.5864661654135, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI45553807, Length=280, Percent_Identity=27.5, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI45553816, Length=280, Percent_Identity=27.5, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24651721, Length=280, Percent_Identity=27.5, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17864154, Length=280, Percent_Identity=27.5, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24652838, Length=172, Percent_Identity=33.7209302325581, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI17137572, Length=172, Percent_Identity=33.7209302325581, Blast_Score=70, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 66048; Mature: 66048

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHIRNFSIIAHIDHGKSTLADRIIQICGGLSDREMEAQVLDSMDLERERGITIKAQTAA
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHCCCEEEEEHHE
LSYRARDGKLYNLNMIDTPGHVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTAI
EEEECCCCEEEEEEEECCCCCCEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
ELGVEVVPVLNKIDLPAANPENAIAEIEDVIGIDAADATPCSAKTGMGVEDVLEALIAKV
HHCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
PPPKGDAGQPLQALIIDSWFDNYVGVVMLVRIVNGTLRPKDRIRLMATGAEYPVEHVGVF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHCEEE
TPKSKNLESLSAGQVGFIIAGIKELTAAKVGDTVTTVKNAAPEPLPGFKEVKPQVFAGLY
CCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCHHHHHHCCCCCCCCCHHHCCHHHHHCCC
PVEANQYDALRESLEKLKLNDASLMYEPEVSQALGFGFRCGFLGLLHMEIVQERLEREFD
CCCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
MDLITTAPTVVYEVVQRDGTTIMVENPAKMPDPSKIEEVREPIVTVNLYMPQDYVGSVIT
CHHHHCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHCCEEEEEEECCHHHHHHHHH
LCTQKRGVQINMQYHGRQVQLTYEIPMGEIVLDFFDRLKSVSRGYASMDYEFKEYRASDV
HHHHCCCEEEEEEECCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCE
VKVDMLINGDKVDALSVIVHRSQSQYRGREVASKMREIIPRQMYDVAIQATIGAHIIARE
EEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHH
NIKALRKNVLAKCYGGDITRKKKLLEKQKEGKKRMKQVGSVEIPQEAFLAILRVEDK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEEECCC
>Mature Secondary Structure
MDHIRNFSIIAHIDHGKSTLADRIIQICGGLSDREMEAQVLDSMDLERERGITIKAQTAA
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHCCCEEEEEHHE
LSYRARDGKLYNLNMIDTPGHVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTAI
EEEECCCCEEEEEEEECCCCCCEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
ELGVEVVPVLNKIDLPAANPENAIAEIEDVIGIDAADATPCSAKTGMGVEDVLEALIAKV
HHCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
PPPKGDAGQPLQALIIDSWFDNYVGVVMLVRIVNGTLRPKDRIRLMATGAEYPVEHVGVF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHCEEE
TPKSKNLESLSAGQVGFIIAGIKELTAAKVGDTVTTVKNAAPEPLPGFKEVKPQVFAGLY
CCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCHHHHHHCCCCCCCCCHHHCCHHHHHCCC
PVEANQYDALRESLEKLKLNDASLMYEPEVSQALGFGFRCGFLGLLHMEIVQERLEREFD
CCCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
MDLITTAPTVVYEVVQRDGTTIMVENPAKMPDPSKIEEVREPIVTVNLYMPQDYVGSVIT
CHHHHCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHCCEEEEEEECCHHHHHHHHH
LCTQKRGVQINMQYHGRQVQLTYEIPMGEIVLDFFDRLKSVSRGYASMDYEFKEYRASDV
HHHHCCCEEEEEEECCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCE
VKVDMLINGDKVDALSVIVHRSQSQYRGREVASKMREIIPRQMYDVAIQATIGAHIIARE
EEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHH
NIKALRKNVLAKCYGGDITRKKKLLEKQKEGKKRMKQVGSVEIPQEAFLAILRVEDK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA