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The map label for this gene is mltB [H]

Identifier: 186475520

GI number: 186475520

Start: 851438

End: 852826

Strand: Reverse

Name: mltB [H]

Synonym: Bphy_0755

Alternate gene names: 186475520

Gene position: 852826-851438 (Counterclockwise)

Preceding gene: 186475523

Following gene: 186475502

Centisome position: 24.51

GC content: 62.63

Gene sequence:

>1389_bases
ATGGCGAGCCTGCGCTGCATGTCTCCGTCCATTGACGCAAGCGGGTTGCGCGAATACTACGAAGTTACCACACGACGCGC
CCAGACCGTGTTGCGGCAACGCTTCTGCACAGTTTCAGGAACCCCGTCGGGTATACTTCCCCGTAACTTTTTTTCGCCCG
CACACATCCAGTCCACCATGACCGTCAAGCTTGCTCCGTCCGCACCACATCGGCTACGCGCAACGACTGTAGCCGCCGCA
CTGTCCATCGCATGGTGCGCGTTCGCGGGAGTCGGCACGGCTTCGGCGCAGAGCGTCAACAGCAAGCCACGCATGCAGCT
CGCGCAGAGTCAACCCGAGCCAGCGGCGCAAGGACAGACGTTCGAAGAAGAGATCGTTCCGCAGCGTTACGCCAACAATG
CCGATGTCGACGCGTTCATCAACGACATGGCCGCGCGCTACGACTTCGACAGCGCCGCACTACATGCGCTGTTTGCACGC
GTCAGCTACTCGGCGACGGCCGTGAAGCTCGTGACACCTTCGCCGACGCCGTCGATCAAGAACTGGCGGGTGTATCAGGC
GCGCTTTCTAGACCCGGTGCGCATCAATGCGGGCGTGAAATTCTGGCGCGCGAACCAGGCAACGCTACAGCGCGCGTACG
AGCAGTACGGCGTACCGCCCGAAGTGATCGTCGGCATCATCGGCGTCGAGACGATCTACGGCCGGTACATGGGCAACTTC
CGCGTGCTCGATGCGCTCACCACGCTCTCGTTCGATTACCCGAACACGCCGAATCGCGCAGAACGGATGATCACGTTCCG
CAAGAATCTCGAAGACTACCTGGTGTGGACGCGCGACTCTCAAATCGATCCGACTACAGTCCTCGGCTCATACACCGGCG
CGATCGGCATTCCGCAGTTTCTGCCGAGCAGCATCGTTCAGTACGCGGTCGACTATGACGGCAACAAGACGATCGATCTG
CGCACGAGCCAGGCCGATGCAATCGGCAGCGTCGCGAATTATCTGAAGCAGAACGGCTGGGAAACGGGTCGTCCCGTCGT
GTGGAACATCGCAACGGATACGGGCAGCCTGGGCATCGCGCAAGCCGCCGCGACAGGCGCGCCGGAGCCGCGCTGGGCGC
TGCAGCAACTGCTGAAGGCGGGCATGCTGATGAACGAGCCGGGTCTCGATACGCGCACGGAAGCGGGCACGCCCGTCACT
GTGGTCGACCTGCCGTCGCCGGGCCGCCCCACGGAATACACGCTCGGTCTGAAAAACTTCTACGTGCTCACGCGCTACAA
CCGCAGCTTCTTCTACGCACTGGCCGTCTACCAGCTCGGCGAGCGCGTGAAGGCGCAGATGGCAGCGAGCGACGCGGGCA
ATGCGTCGATGCCCGCGGCGTCGCAGTAA

Upstream 100 bases:

>100_bases
CATTCGGGATGCCATTGACCCATCTCATGCTGCAGACAGTCTGCATGTGAATAGAAACCGGTTGCCATGAGTTGATTTTC
AGCTGCGCGCCTCGCACGGC

Downstream 100 bases:

>100_bases
GCACACTCGCGCACAACCATGAAAAACGCCGGCAGATGCCGGCGTTTGTCTTTATCTGGATGCGAAGGCCGCTCCCACGC
GGCACCGCGACTCAGGCGGG

Product: lytic murein transglycosylase B

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MASLRCMSPSIDASGLREYYEVTTRRAQTVLRQRFCTVSGTPSGILPRNFFSPAHIQSTMTVKLAPSAPHRLRATTVAAA
LSIAWCAFAGVGTASAQSVNSKPRMQLAQSQPEPAAQGQTFEEEIVPQRYANNADVDAFINDMAARYDFDSAALHALFAR
VSYSATAVKLVTPSPTPSIKNWRVYQARFLDPVRINAGVKFWRANQATLQRAYEQYGVPPEVIVGIIGVETIYGRYMGNF
RVLDALTTLSFDYPNTPNRAERMITFRKNLEDYLVWTRDSQIDPTTVLGSYTGAIGIPQFLPSSIVQYAVDYDGNKTIDL
RTSQADAIGSVANYLKQNGWETGRPVVWNIATDTGSLGIAQAAATGAPEPRWALQQLLKAGMLMNEPGLDTRTEAGTPVT
VVDLPSPGRPTEYTLGLKNFYVLTRYNRSFFYALAVYQLGERVKAQMAASDAGNASMPAASQ

Sequences:

>Translated_462_residues
MASLRCMSPSIDASGLREYYEVTTRRAQTVLRQRFCTVSGTPSGILPRNFFSPAHIQSTMTVKLAPSAPHRLRATTVAAA
LSIAWCAFAGVGTASAQSVNSKPRMQLAQSQPEPAAQGQTFEEEIVPQRYANNADVDAFINDMAARYDFDSAALHALFAR
VSYSATAVKLVTPSPTPSIKNWRVYQARFLDPVRINAGVKFWRANQATLQRAYEQYGVPPEVIVGIIGVETIYGRYMGNF
RVLDALTTLSFDYPNTPNRAERMITFRKNLEDYLVWTRDSQIDPTTVLGSYTGAIGIPQFLPSSIVQYAVDYDGNKTIDL
RTSQADAIGSVANYLKQNGWETGRPVVWNIATDTGSLGIAQAAATGAPEPRWALQQLLKAGMLMNEPGLDTRTEAGTPVT
VVDLPSPGRPTEYTLGLKNFYVLTRYNRSFFYALAVYQLGERVKAQMAASDAGNASMPAASQ
>Mature_461_residues
ASLRCMSPSIDASGLREYYEVTTRRAQTVLRQRFCTVSGTPSGILPRNFFSPAHIQSTMTVKLAPSAPHRLRATTVAAAL
SIAWCAFAGVGTASAQSVNSKPRMQLAQSQPEPAAQGQTFEEEIVPQRYANNADVDAFINDMAARYDFDSAALHALFARV
SYSATAVKLVTPSPTPSIKNWRVYQARFLDPVRINAGVKFWRANQATLQRAYEQYGVPPEVIVGIIGVETIYGRYMGNFR
VLDALTTLSFDYPNTPNRAERMITFRKNLEDYLVWTRDSQIDPTTVLGSYTGAIGIPQFLPSSIVQYAVDYDGNKTIDLR
TSQADAIGSVANYLKQNGWETGRPVVWNIATDTGSLGIAQAAATGAPEPRWALQQLLKAGMLMNEPGLDTRTEAGTPVTV
VDLPSPGRPTEYTLGLKNFYVLTRYNRSFFYALAVYQLGERVKAQMAASDAGNASMPAASQ

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=325, Percent_Identity=40.3076923076923, Blast_Score=213, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 50589; Mature: 50458

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASLRCMSPSIDASGLREYYEVTTRRAQTVLRQRFCTVSGTPSGILPRNFFSPAHIQSTM
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCEEE
TVKLAPSAPHRLRATTVAAALSIAWCAFAGVGTASAQSVNSKPRMQLAQSQPEPAAQGQT
EEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHCCCCCCCCCCC
FEEEIVPQRYANNADVDAFINDMAARYDFDSAALHALFARVSYSATAVKLVTPSPTPSIK
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCC
NWRVYQARFLDPVRINAGVKFWRANQATLQRAYEQYGVPPEVIVGIIGVETIYGRYMGNF
CCEEEHHHCCCCEEECCCCEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
RVLDALTTLSFDYPNTPNRAERMITFRKNLEDYLVWTRDSQIDPTTVLGSYTGAIGIPQF
CEEHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHEEEECCCCCCCHHHHCCCCCCCCCHHH
LPSSIVQYAVDYDGNKTIDLRTSQADAIGSVANYLKQNGWETGRPVVWNIATDTGSLGIA
HHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHH
QAAATGAPEPRWALQQLLKAGMLMNEPGLDTRTEAGTPVTVVDLPSPGRPTEYTLGLKNF
HHHHCCCCCHHHHHHHHHHCCCEECCCCCCCCCCCCCCEEEEECCCCCCCCEEEECCCEE
YVLTRYNRSFFYALAVYQLGERVKAQMAASDAGNASMPAASQ
EEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
ASLRCMSPSIDASGLREYYEVTTRRAQTVLRQRFCTVSGTPSGILPRNFFSPAHIQSTM
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCEEE
TVKLAPSAPHRLRATTVAAALSIAWCAFAGVGTASAQSVNSKPRMQLAQSQPEPAAQGQT
EEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHCCCCCCCCCCC
FEEEIVPQRYANNADVDAFINDMAARYDFDSAALHALFARVSYSATAVKLVTPSPTPSIK
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCC
NWRVYQARFLDPVRINAGVKFWRANQATLQRAYEQYGVPPEVIVGIIGVETIYGRYMGNF
CCEEEHHHCCCCEEECCCCEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
RVLDALTTLSFDYPNTPNRAERMITFRKNLEDYLVWTRDSQIDPTTVLGSYTGAIGIPQF
CEEHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHEEEECCCCCCCHHHHCCCCCCCCCHHH
LPSSIVQYAVDYDGNKTIDLRTSQADAIGSVANYLKQNGWETGRPVVWNIATDTGSLGIA
HHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHH
QAAATGAPEPRWALQQLLKAGMLMNEPGLDTRTEAGTPVTVVDLPSPGRPTEYTLGLKNF
HHHHCCCCCHHHHHHHHHHCCCEECCCCCCCCCCCCCCEEEEECCCCCCCCEEEECCCEE
YVLTRYNRSFFYALAVYQLGERVKAQMAASDAGNASMPAASQ
EEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]