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The map label for this gene is nuoF2 [H]

Identifier: 186475493

GI number: 186475493

Start: 822588

End: 824144

Strand: Reverse

Name: nuoF2 [H]

Synonym: Bphy_0728

Alternate gene names: 186475493

Gene position: 824144-822588 (Counterclockwise)

Preceding gene: 186475494

Following gene: 186475492

Centisome position: 23.69

GC content: 65.0

Gene sequence:

>1557_bases
ATGACGCGCATCTATGTTCCTCGCGATTCTTCCGCGCTGGCGCTCGGCGCAGATGCGCTCGCCGCGGCCATCGTCGATGA
AGCGAAACAACGCGGCATCGACATCGAACTCGTGCGCAACGGCTCGCGCGGGTTGCTGTATCTCGAACCGCTCGTCGAAG
TCGAGACGCCCGAAGGCCGCATCGGCTATGCGAACGTCGAAGCCGACGACGTGAAGGCGCTATTCGACGCAGGCTTCATC
GAAGGCAAGACGCACGCGAAGCAGGTCGGCGTCGTCGACGAGATTCCGTATCTGAAGAAGCAGCAGCGCCTGACGTTCGC
GCGCATCGGCATCACCGACCCGCTCTCGACGGACGACTACATCGCCAACGGCGGCATCGTCGGCCTGTACAACGCGCTTG
CGATGACGGGCGACGCCGCCGTCGAAGCGCTCATCGAATCCGGCCTGCGCGGCCGCGGCGGCGCAGCGTTCCCGGCGGGC
ATCAAGTGGCGCACGGTGCGCGCCGCGAACGCCGATCAGAAGTACATCGTCTGCAATGCGGACGAAGGCGACTCGGGCAC
GTTCTCCGACCGCCTCGTGATGGAAAGCGATCCGTTCGTGCTGATCGAAGGGATGATCATCGCGGGCATCGTGACGGGCG
CGACGGTCGGCCATATCTATGTGCGCAGCGAGTATCCGCATTCGATCGCGACGCTCGAAGAAGCTATCGGCAAGGCGCGC
GCCGCCGGTTGGCTCGGCGACAGCGTGCTCGGTTCGGAACACCGCTTCGAGCTATTCGTCGCGAAGGGTGCGGGCGCTTA
TGTGTGCGGCGAAGAAACGGCGCTGCTCGAATCGCTCGAAGGCAAGCGCGGCATCGTGCGCGCCAAGCCGCCGCTGCCCG
CGCTCGAGGGTCTGTTCGGCAAGCCGACTGTCATCAACAACGTGATCACCCTCGCCACGGTGCCGATCATCTTCGCTAAG
GGTGCCGCGTTCTACAAGGACTTCGGCATGGGCCGCTCGCGCGGTACGCTGCCGTTCCAGATCGCGGGCAACGTGAAGCA
AGGCGGTCTCGTGGAACTCGCGTTCGGCTGCACGCTGCGCGAACTGATGTTCGACTATGGCGGCGGCACGGCGAGCGGCC
GACCCGCGCGCGCGGTTCAGGTAGGCGGCCCGCTCGGCACGTATCTGCCCGAAAGCCAGTGGGACATTCCGCTCGACTAC
GAGGAATACGCGAAAGTCGGTGCCGTCGTCGGTCACGGCGGTCTCGTGATTCACGACGACACGTCGAATCTCGCCGAGCT
CGCGCAATACGCCATGCACTTCTGCGCACTCGAATCGTGCGGTAAGTGCACGCCCTGCCGGATTGGCTCGACGCGCGGCG
TCGAAGTGATCCAGCGCATCCGCAACGGCGACACGTCGACGAAGCAGGTGCAACTGCTGCGCGACCTGTGCGACACCATG
GTGTCCGGTTCGCTGTGCGCGATGGGCGGCATGACGCCGTTTCCGGTCGTCTCCGCGCTCGACCATTTCCCCGAAGACTT
CGGTCTCGCGACCGTCACGCCAAAAGAAGCCGCGTAA

Upstream 100 bases:

>100_bases
CCCCCGCCATGATGATCAACGGCGAACTGCACGCCAGGGTCACGCCGCAGAAGTTCGACGCGCTGCTTGCAGCCGCCCTG
AAACGCGTGGAGGAAACGGC

Downstream 100 bases:

>100_bases
TTAGGGAGCCCAAGATGTCCGATCCGATCACTTTCCAATCCGGCGGCTGCGGCTCCGGCAACTGCGCCTGCAAGGCAGGC
GCGCTGCGCAATGAACGCGG

Product: NADH dehydrogenase (quinone)

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MTRIYVPRDSSALALGADALAAAIVDEAKQRGIDIELVRNGSRGLLYLEPLVEVETPEGRIGYANVEADDVKALFDAGFI
EGKTHAKQVGVVDEIPYLKKQQRLTFARIGITDPLSTDDYIANGGIVGLYNALAMTGDAAVEALIESGLRGRGGAAFPAG
IKWRTVRAANADQKYIVCNADEGDSGTFSDRLVMESDPFVLIEGMIIAGIVTGATVGHIYVRSEYPHSIATLEEAIGKAR
AAGWLGDSVLGSEHRFELFVAKGAGAYVCGEETALLESLEGKRGIVRAKPPLPALEGLFGKPTVINNVITLATVPIIFAK
GAAFYKDFGMGRSRGTLPFQIAGNVKQGGLVELAFGCTLRELMFDYGGGTASGRPARAVQVGGPLGTYLPESQWDIPLDY
EEYAKVGAVVGHGGLVIHDDTSNLAELAQYAMHFCALESCGKCTPCRIGSTRGVEVIQRIRNGDTSTKQVQLLRDLCDTM
VSGSLCAMGGMTPFPVVSALDHFPEDFGLATVTPKEAA

Sequences:

>Translated_518_residues
MTRIYVPRDSSALALGADALAAAIVDEAKQRGIDIELVRNGSRGLLYLEPLVEVETPEGRIGYANVEADDVKALFDAGFI
EGKTHAKQVGVVDEIPYLKKQQRLTFARIGITDPLSTDDYIANGGIVGLYNALAMTGDAAVEALIESGLRGRGGAAFPAG
IKWRTVRAANADQKYIVCNADEGDSGTFSDRLVMESDPFVLIEGMIIAGIVTGATVGHIYVRSEYPHSIATLEEAIGKAR
AAGWLGDSVLGSEHRFELFVAKGAGAYVCGEETALLESLEGKRGIVRAKPPLPALEGLFGKPTVINNVITLATVPIIFAK
GAAFYKDFGMGRSRGTLPFQIAGNVKQGGLVELAFGCTLRELMFDYGGGTASGRPARAVQVGGPLGTYLPESQWDIPLDY
EEYAKVGAVVGHGGLVIHDDTSNLAELAQYAMHFCALESCGKCTPCRIGSTRGVEVIQRIRNGDTSTKQVQLLRDLCDTM
VSGSLCAMGGMTPFPVVSALDHFPEDFGLATVTPKEAA
>Mature_517_residues
TRIYVPRDSSALALGADALAAAIVDEAKQRGIDIELVRNGSRGLLYLEPLVEVETPEGRIGYANVEADDVKALFDAGFIE
GKTHAKQVGVVDEIPYLKKQQRLTFARIGITDPLSTDDYIANGGIVGLYNALAMTGDAAVEALIESGLRGRGGAAFPAGI
KWRTVRAANADQKYIVCNADEGDSGTFSDRLVMESDPFVLIEGMIIAGIVTGATVGHIYVRSEYPHSIATLEEAIGKARA
AGWLGDSVLGSEHRFELFVAKGAGAYVCGEETALLESLEGKRGIVRAKPPLPALEGLFGKPTVINNVITLATVPIIFAKG
AAFYKDFGMGRSRGTLPFQIAGNVKQGGLVELAFGCTLRELMFDYGGGTASGRPARAVQVGGPLGTYLPESQWDIPLDYE
EYAKVGAVVGHGGLVIHDDTSNLAELAQYAMHFCALESCGKCTPCRIGSTRGVEVIQRIRNGDTSTKQVQLLRDLCDTMV
SGSLCAMGGMTPFPVVSALDHFPEDFGLATVTPKEAA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260656005, Length=363, Percent_Identity=37.7410468319559, Blast_Score=248, Evalue=1e-65,
Organism=Homo sapiens, GI20149568, Length=363, Percent_Identity=37.7410468319559, Blast_Score=248, Evalue=1e-65,
Organism=Escherichia coli, GI1788620, Length=407, Percent_Identity=39.0663390663391, Blast_Score=296, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI17531727, Length=420, Percent_Identity=35.7142857142857, Blast_Score=260, Evalue=1e-69,
Organism=Drosophila melanogaster, GI19920794, Length=372, Percent_Identity=37.0967741935484, Blast_Score=253, Evalue=3e-67,
Organism=Drosophila melanogaster, GI45550452, Length=368, Percent_Identity=36.9565217391304, Blast_Score=236, Evalue=2e-62,
Organism=Drosophila melanogaster, GI24653819, Length=353, Percent_Identity=29.4617563739377, Blast_Score=177, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24653817, Length=332, Percent_Identity=27.4096385542169, Blast_Score=152, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 54938; Mature: 54807

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00644 COMPLEX1_51K_1 ; PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRIYVPRDSSALALGADALAAAIVDEAKQRGIDIELVRNGSRGLLYLEPLVEVETPEGR
CCEEEECCCCCCEEECHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEECEEEEECCCCC
IGYANVEADDVKALFDAGFIEGKTHAKQVGVVDEIPYLKKQQRLTFARIGITDPLSTDDY
EEEECCCHHHHHHHHHCCCCCCCCHHHHCCCHHCCCHHHHHHCCEEEEECCCCCCCCCCE
IANGGIVGLYNALAMTGDAAVEALIESGLRGRGGAAFPAGIKWRTVRAANADQKYIVCNA
ECCCCEEHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEC
DEGDSGTFSDRLVMESDPFVLIEGMIIAGIVTGATVGHIYVRSEYPHSIATLEEAIGKAR
CCCCCCCCCCEEEECCCCEEEECCHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHHH
AAGWLGDSVLGSEHRFELFVAKGAGAYVCGEETALLESLEGKRGIVRAKPPLPALEGLFG
HHCCCCCHHCCCCCEEEEEEECCCCEEEECCHHHHHHHCCCCCCEEECCCCCHHHHCCCC
KPTVINNVITLATVPIIFAKGAAFYKDFGMGRSRGTLPFQIAGNVKQGGLVELAFGCTLR
CCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCEEEEECCCCCCCEEEEECCCHHH
ELMFDYGGGTASGRPARAVQVGGPLGTYLPESQWDIPLDYEEYAKVGAVVGHGGLVIHDD
HHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHEEECCCEEEECC
TSNLAELAQYAMHFCALESCGKCTPCRIGSTRGVEVIQRIRNGDTSTKQVQLLRDLCDTM
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHH
VSGSLCAMGGMTPFPVVSALDHFPEDFGLATVTPKEAA
HCCCEECCCCCCHHHHHHHHHHCCHHCCEEECCCCCCC
>Mature Secondary Structure 
TRIYVPRDSSALALGADALAAAIVDEAKQRGIDIELVRNGSRGLLYLEPLVEVETPEGR
CEEEECCCCCCEEECHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEECEEEEECCCCC
IGYANVEADDVKALFDAGFIEGKTHAKQVGVVDEIPYLKKQQRLTFARIGITDPLSTDDY
EEEECCCHHHHHHHHHCCCCCCCCHHHHCCCHHCCCHHHHHHCCEEEEECCCCCCCCCCE
IANGGIVGLYNALAMTGDAAVEALIESGLRGRGGAAFPAGIKWRTVRAANADQKYIVCNA
ECCCCEEHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEC
DEGDSGTFSDRLVMESDPFVLIEGMIIAGIVTGATVGHIYVRSEYPHSIATLEEAIGKAR
CCCCCCCCCCEEEECCCCEEEECCHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHHH
AAGWLGDSVLGSEHRFELFVAKGAGAYVCGEETALLESLEGKRGIVRAKPPLPALEGLFG
HHCCCCCHHCCCCCEEEEEEECCCCEEEECCHHHHHHHCCCCCCEEECCCCCHHHHCCCC
KPTVINNVITLATVPIIFAKGAAFYKDFGMGRSRGTLPFQIAGNVKQGGLVELAFGCTLR
CCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCEEEEECCCCCCCEEEEECCCHHH
ELMFDYGGGTASGRPARAVQVGGPLGTYLPESQWDIPLDYEEYAKVGAVVGHGGLVIHDD
HHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHEEECCCEEEECC
TSNLAELAQYAMHFCALESCGKCTPCRIGSTRGVEVIQRIRNGDTSTKQVQLLRDLCDTM
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHH
VSGSLCAMGGMTPFPVVSALDHFPEDFGLATVTPKEAA
HCCCEECCCCCCHHHHHHHHHHCCHHCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]