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The map label for this gene is gatA [H]

Identifier: 186475480

GI number: 186475480

Start: 804264

End: 805652

Strand: Reverse

Name: gatA [H]

Synonym: Bphy_0715

Alternate gene names: 186475480

Gene position: 805652-804264 (Counterclockwise)

Preceding gene: 186475484

Following gene: 186475479

Centisome position: 23.16

GC content: 71.35

Gene sequence:

>1389_bases
ATGAGCACCGCCATGTCACCCGAACCCACGCCGTTTCCCCCGCTCGCCCAACTCGCTGCCGATCTCGCCTCCGGCCGCAC
CACGAGCCGCGCGCTCGTCGAAGCCGCGCTGGCGCGGATCGCCGACCCCGCTGGCCAGGGGGCGGCCGTATTCATGCAGG
TCGACGCCGATAACGCGCGCGCCACGGCCGATGCGCACGATCGCCTACGCGCAGCCGGCACCGTACTGTCGCCGCTCGCG
GGCATTCCCGTCTCCGTGAAAGACCTGTTCGACGTCGAGGGACAGGTGACGCGCGCCGGCTCGACAGTGCTGGCGAACGC
GGCACCCGCCACGGCAGACGCCCCGGCGGTCGCGCGCCTGAAGCGCGCGGGCGCCGTCATCGTCGGTCGCACGAACATGA
GCGAGTTCGCGTTCTCGGGGCTCGGCCTCAACCCGCACTACGGCAATCCCCTCTCGCCCTACCGGCGCGGCGTGAAGGGC
GACGAGCGGGTGTCGGGCGGCTCGTCGTCGGGGGCAGCAGCGTCCGTCGCGGACGGCATGGCGGCCGTCGCGCTCGGCAG
CGACACGGGCGGCTCGCTGCGCATTCCCGCCGCGCTGTGCGGACTTACCGGCTTCAAGCCGACGGCCGACCGCGTGCCCA
AACAGGGCGGCGTACCGCTCTCGCCGACACTTGATGCATTCGGCGCGATCGGCGCGACAGTCGCGTGCTGCGCACTGGTC
GACCGGATCCTCGCCGGGCTGGAGCCCGTCATTCCGGCTGCGCGGCCGCTCGAGGGCGCGCGGCTCGGCGTGCTGAACCA
TTACGTGACGGACGGCATCGAGCCGGAAGTCGCCGAGGCTTATGACGCCGCGCTCAAGCATCTGGAGGCGGCGGGCGCGA
TCGTCAGCGACGTGCGTTTTGCGCCGCTCGACCGGCTTGCCGAAATCAACCGCTTCGGCTTTTCGCCCATCGAAGCCTAC
GCGTGGCACCGGCCGCTGCTCGACGCGCACCGGGACGCCTACGATCCGCGCGTGTTGACGCGCATCCTCAAAGGCCAGCC
GGCGACGGCCGCCGACTATCTCGACCTGCTCGCCGGGCGCGCCGCGGTGCTGGCCGAGGCGCGCACGATGTGGCAGCGCT
TCGATGCCCTCGTCGCGCCGACGGTGCCCGTCGTGCCGCCGCGCGTCGCCGAACTGGTGCACGACGACGATGCATTCTTT
CGTGCCAACGGCCTCGTGCTGCGCAATCCGAGCGCCTTCAATTTTCTCGATGCCTGCGCGATCTCTGTGCCGTGTCATCC
GCGCGGCGGCGCGCCCGTCGGGCTGATGCTGGCCGCCGCGCCGCACGCGGACGACGCGCTGCTGGGAATTGGACGAGCCG
TCGAAGCGGTGTTGAACACCATCCGCTGA

Upstream 100 bases:

>100_bases
GCTCGCCCGCAGTCATCACAGATTCCGCGCGACGCATGACGCCGAAGCCGCGCGAAGCGTCACAATACGGATCCCGACTA
CCGACCTGCCGATTCACGCC

Downstream 100 bases:

>100_bases
CATGCGCCGCGGATCGCGCACGGCCGTGCCTGGGCGGCGGCATGCGCCGCGGGCTTCGGCTAGAATCGCCGCACCCTTTA
CCGAACTTCCACCGCCAGCA

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MSTAMSPEPTPFPPLAQLAADLASGRTTSRALVEAALARIADPAGQGAAVFMQVDADNARATADAHDRLRAAGTVLSPLA
GIPVSVKDLFDVEGQVTRAGSTVLANAAPATADAPAVARLKRAGAVIVGRTNMSEFAFSGLGLNPHYGNPLSPYRRGVKG
DERVSGGSSSGAAASVADGMAAVALGSDTGGSLRIPAALCGLTGFKPTADRVPKQGGVPLSPTLDAFGAIGATVACCALV
DRILAGLEPVIPAARPLEGARLGVLNHYVTDGIEPEVAEAYDAALKHLEAAGAIVSDVRFAPLDRLAEINRFGFSPIEAY
AWHRPLLDAHRDAYDPRVLTRILKGQPATAADYLDLLAGRAAVLAEARTMWQRFDALVAPTVPVVPPRVAELVHDDDAFF
RANGLVLRNPSAFNFLDACAISVPCHPRGGAPVGLMLAAAPHADDALLGIGRAVEAVLNTIR

Sequences:

>Translated_462_residues
MSTAMSPEPTPFPPLAQLAADLASGRTTSRALVEAALARIADPAGQGAAVFMQVDADNARATADAHDRLRAAGTVLSPLA
GIPVSVKDLFDVEGQVTRAGSTVLANAAPATADAPAVARLKRAGAVIVGRTNMSEFAFSGLGLNPHYGNPLSPYRRGVKG
DERVSGGSSSGAAASVADGMAAVALGSDTGGSLRIPAALCGLTGFKPTADRVPKQGGVPLSPTLDAFGAIGATVACCALV
DRILAGLEPVIPAARPLEGARLGVLNHYVTDGIEPEVAEAYDAALKHLEAAGAIVSDVRFAPLDRLAEINRFGFSPIEAY
AWHRPLLDAHRDAYDPRVLTRILKGQPATAADYLDLLAGRAAVLAEARTMWQRFDALVAPTVPVVPPRVAELVHDDDAFF
RANGLVLRNPSAFNFLDACAISVPCHPRGGAPVGLMLAAAPHADDALLGIGRAVEAVLNTIR
>Mature_461_residues
STAMSPEPTPFPPLAQLAADLASGRTTSRALVEAALARIADPAGQGAAVFMQVDADNARATADAHDRLRAAGTVLSPLAG
IPVSVKDLFDVEGQVTRAGSTVLANAAPATADAPAVARLKRAGAVIVGRTNMSEFAFSGLGLNPHYGNPLSPYRRGVKGD
ERVSGGSSSGAAASVADGMAAVALGSDTGGSLRIPAALCGLTGFKPTADRVPKQGGVPLSPTLDAFGAIGATVACCALVD
RILAGLEPVIPAARPLEGARLGVLNHYVTDGIEPEVAEAYDAALKHLEAAGAIVSDVRFAPLDRLAEINRFGFSPIEAYA
WHRPLLDAHRDAYDPRVLTRILKGQPATAADYLDLLAGRAAVLAEARTMWQRFDALVAPTVPVVPPRVAELVHDDDAFFR
ANGLVLRNPSAFNFLDACAISVPCHPRGGAPVGLMLAAAPHADDALLGIGRAVEAVLNTIR

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=313, Percent_Identity=30.6709265175719, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI195972892, Length=228, Percent_Identity=31.5789473684211, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI166795287, Length=138, Percent_Identity=37.6811594202899, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17543272, Length=438, Percent_Identity=26.4840182648402, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17538252, Length=304, Percent_Identity=28.6184210526316, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17537465, Length=371, Percent_Identity=27.7628032345013, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71990152, Length=170, Percent_Identity=36.4705882352941, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17556264, Length=250, Percent_Identity=30, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17556276, Length=259, Percent_Identity=32.4324324324324, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=259, Percent_Identity=32.4324324324324, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17538254, Length=214, Percent_Identity=29.4392523364486, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=383, Percent_Identity=31.5926892950392, Blast_Score=124, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6323950, Length=159, Percent_Identity=38.9937106918239, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24648113, Length=499, Percent_Identity=27.0541082164329, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI45550774, Length=215, Percent_Identity=33.4883720930233, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648435, Length=215, Percent_Identity=33.4883720930233, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24648437, Length=215, Percent_Identity=33.4883720930233, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24648441, Length=146, Percent_Identity=39.041095890411, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648439, Length=146, Percent_Identity=39.041095890411, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356731, Length=315, Percent_Identity=29.2063492063492, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI161078093, Length=247, Percent_Identity=29.9595141700405, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24644968, Length=497, Percent_Identity=24.3460764587525, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 47598; Mature: 47466

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTAMSPEPTPFPPLAQLAADLASGRTTSRALVEAALARIADPAGQGAAVFMQVDADNAR
CCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCC
ATADAHDRLRAAGTVLSPLAGIPVSVKDLFDVEGQVTRAGSTVLANAAPATADAPAVARL
CCCCHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCEEECCCEEEECCCCCCCCCHHHHHH
KRAGAVIVGRTNMSEFAFSGLGLNPHYGNPLSPYRRGVKGDERVSGGSSSGAAASVADGM
HHCCEEEEECCCHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHCCC
AAVALGSDTGGSLRIPAALCGLTGFKPTADRVPKQGGVPLSPTLDAFGAIGATVACCALV
EEEEECCCCCCCEECHHHHHCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
DRILAGLEPVIPAARPLEGARLGVLNHYVTDGIEPEVAEAYDAALKHLEAAGAIVSDVRF
HHHHHCCHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
APLDRLAEINRFGFSPIEAYAWHRPLLDAHRDAYDPRVLTRILKGQPATAADYLDLLAGR
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHH
AAVLAEARTMWQRFDALVAPTVPVVPPRVAELVHDDDAFFRANGLVLRNPSAFNFLDACA
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCEEEECCEEEECCCHHHHHHHHE
ISVPCHPRGGAPVGLMLAAAPHADDALLGIGRAVEAVLNTIR
EECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STAMSPEPTPFPPLAQLAADLASGRTTSRALVEAALARIADPAGQGAAVFMQVDADNAR
CCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCC
ATADAHDRLRAAGTVLSPLAGIPVSVKDLFDVEGQVTRAGSTVLANAAPATADAPAVARL
CCCCHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCEEECCCEEEECCCCCCCCCHHHHHH
KRAGAVIVGRTNMSEFAFSGLGLNPHYGNPLSPYRRGVKGDERVSGGSSSGAAASVADGM
HHCCEEEEECCCHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHCCC
AAVALGSDTGGSLRIPAALCGLTGFKPTADRVPKQGGVPLSPTLDAFGAIGATVACCALV
EEEEECCCCCCCEECHHHHHCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
DRILAGLEPVIPAARPLEGARLGVLNHYVTDGIEPEVAEAYDAALKHLEAAGAIVSDVRF
HHHHHCCHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
APLDRLAEINRFGFSPIEAYAWHRPLLDAHRDAYDPRVLTRILKGQPATAADYLDLLAGR
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHH
AAVLAEARTMWQRFDALVAPTVPVVPPRVAELVHDDDAFFRANGLVLRNPSAFNFLDACA
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCEEEECCEEEECCCHHHHHHHHE
ISVPCHPRGGAPVGLMLAAAPHADDALLGIGRAVEAVLNTIR
EECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA