Click here to switch to the map view.

The map label for this gene is spoT [H]

Identifier: 186475437

GI number: 186475437

Start: 755774

End: 758158

Strand: Direct

Name: spoT [H]

Synonym: Bphy_0671

Alternate gene names: 186475437

Gene position: 755774-758158 (Clockwise)

Preceding gene: 186475436

Following gene: 186475438

Centisome position: 21.72

GC content: 62.68

Gene sequence:

>2385_bases
ATGAGCACCAATCCCTCGCCATCCGCCACGGAAGTGGATCACGCTGCCCACGAACACGATAGCGACTCGCCATCGTCTGC
ACGCAAGTACATCGACGCGGTCCTCGAACAATCGTTTCGCCACCTGTTCGGACCGACCGCCACGCCGGAGCAGCCGCGCC
GGCATGATGTCGTCTCCATCGCCAAGCTGACTACTGCGCTGTCCGGCTATCTGAGCCCGGAAGAGATCAAGGAAGTCAAA
GCGGCTTTCCACTTCAGCGACGAAGCCCACCTCGGGCAATATCGTCAAAGCGGCGAACCCTACATCACGCATCCTGTTGC
CGTCGCGGAAACGTGCGCGGCGTGGAAGCTCGATGCGCAGTCGATCATGGCGGCGCTCCTGCACGACGTGATGGAAGATC
AGGGCGTGACCAAGACCGAACTGGCAGAGCGCTTCGGCGCGAAGGTCGCGGAACTGGTCGACGGGTTGTCGAAGCTGGAC
AAGATGGAGTTCCGCAGTCGCGAGGAAGCGCAGGCGGAAAACTTCCGCAAGATGCTGCTCGCGATGGCGCGCGATGTGCG
GGTGATCCTCGTGAAGCTCGCGGACCGGCTGCACAACATGCGCACGCTCGGCGCGGTGCCGCCCGAAAAGCGCCGCCGCG
TGGCGCGCGAAACGCTGGACATCTACGCGCCGATCGCACACCGTCTCGGCCTGAACAACACCTATCGCGAGTTGCAGGAC
CTGAGCTTCGCGAACTTCAATCCGCACCGTTACGCGACGCTCGAAAAAGCCGTGAAGGCGGCGCGCGGCAACCGTCGTGA
AGTGGTCGGCAAGATTCTCGAATCGGTGCAGCGCGCCATTGCCGACGCGAAGCTCGATGCCGAAGTGACGGGCCGCGAGA
AAACGATCTTCAGCATCTACAAAAAGATGCGCGACAAGCAGCTGTCGTTCTCACAGGTGCTCGACGTGTACGGTTTCCGC
GTGGTGGTCGAAAGCCCGCTCGAATGCTATACGTGCCTGGGCGCGCTGCATGCGCTTTACAAGCCGGTGCCGGGCAAGTT
CAAGGACTACATCGCGATTCCGAAGGTCAACGGCTATCAGTCCCTGCATACCACCCTGGTCGGCCCATTCGGCGCGCCGA
TCGAGTTCCAGGTGCGCACGCGCAAGATGCACGAGATCGCGGAAGCGGGTGTCGCCGCGCACTGGCTGTACAAGAACGGC
GGCGCGGACCTGAACGATGTGCAGAAGCGCGCGCATCAATGGCTCAAGTCGCTGCTCGACATTCAGAGCGAAGTCGGCGA
TTCGAGCGAATTCCTCGAACACGTGAAGATCGATCTCTTCCCGGATGCCGTCTACGTGTTCACGCCGAAGTCGAAGATCA
TGGCGCTGCCGCGCGGTGCGACGGCTCTGGACTTCGCGTATTCGATCCACAGCGACCTGGGCAATCAGTGCGTCGCGGTG
AAGATCAACAACGAACTGCTGCCGCTGCGTACCGAGCTGAAGAGCGGCGATATCGTCGAAGTGATCACGGCGCCGTATTC
AAAACCGAACCCCGCGTGGCTTGGCTTCGTGCGCACGGGCAAGGCGCGCACCGCGATCCGTCATTACCTGAAGACGATGC
GTCTTAACGAATCGGTGCAACTCGGTGAGCGTCTCGTCGATCAGTCGCTCAAGGGTTATGGGCTCGCGCTGTCCGACGTC
ACGCCGGAAGTGTGGGACAAGCTCGTGCTGTGGACGGGCAACAAGAACCGTCAGGAAATCTTCGCAGATATAGGTCTCGG
CCGGCGCGTGGCCGCCGTGATGGCCAAGCGCATCGAAGTGCTGATGAACGGCCGCGAAGGTCACGAAGGCGACGACGACC
ATCCGCGCGCCGAGCATCCGACGCCCAACGCGCCACCCGTCGTAATCACGGGCACCGAGGGGATGTCGGTGCAACTGTCC
GCATGCTGCCGCCCGATCCCCGGCGACGACATCATGGGCTACATCGGCATCGGTCTCGGCATGGCCATCCACACCACGGA
TTGCCGCGTCGCTCAGCGCATTCATCGGCGCGATCCGGGCCGCTGGATCGACGTCGCGTGGGCGCCGCAACCGGGGCGCC
TGTTCGACGTCGCGATCAAGGTGCTGGTGAAGAACACCAAGGGCGTGTTTGCGCGTGTCGCCGCGGATATCACGTCGGCC
GACGCCAACATCGTCCACATCGCAATGGACGAAGACCAGTCGCAGGAATCAACGGTGCTGCGCTTCGTCATCCAGGTCAG
CGACCGCGTGCATCTCGCCAACGTGATGCGCCGCGTGCGGACCAATCTCGACGTGATGCGCATCGCGCGCGAACGGCCGA
GCGAAGAAGGGCATCGTCATCACGACGGCGGCATGCGGATCGATCGCGAGCGCGCCGATTACTGA

Upstream 100 bases:

>100_bases
AATGCTGAAGAAGGTGCCTGTCTAAGGCACGCTTCAACGTTTGAACGTCATGTAACTCACCCCACGCGTGCAGACGGGCA
CTATCGCAACGGAGGCGACC

Downstream 100 bases:

>100_bases
AATCGGAAGTTGAAAGGCGGGCGCGTCGCTTGCGACTGGTTGCACGAAGGCGCGCCGAGCTTTGCCTGTCTACAGGAACA
ACGCGATGAACGTAGCTGAT

Product: (p)ppGpp synthetase I SpoT/RelA

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 794; Mature: 793

Protein sequence:

>794_residues
MSTNPSPSATEVDHAAHEHDSDSPSSARKYIDAVLEQSFRHLFGPTATPEQPRRHDVVSIAKLTTALSGYLSPEEIKEVK
AAFHFSDEAHLGQYRQSGEPYITHPVAVAETCAAWKLDAQSIMAALLHDVMEDQGVTKTELAERFGAKVAELVDGLSKLD
KMEFRSREEAQAENFRKMLLAMARDVRVILVKLADRLHNMRTLGAVPPEKRRRVARETLDIYAPIAHRLGLNNTYRELQD
LSFANFNPHRYATLEKAVKAARGNRREVVGKILESVQRAIADAKLDAEVTGREKTIFSIYKKMRDKQLSFSQVLDVYGFR
VVVESPLECYTCLGALHALYKPVPGKFKDYIAIPKVNGYQSLHTTLVGPFGAPIEFQVRTRKMHEIAEAGVAAHWLYKNG
GADLNDVQKRAHQWLKSLLDIQSEVGDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYSIHSDLGNQCVAV
KINNELLPLRTELKSGDIVEVITAPYSKPNPAWLGFVRTGKARTAIRHYLKTMRLNESVQLGERLVDQSLKGYGLALSDV
TPEVWDKLVLWTGNKNRQEIFADIGLGRRVAAVMAKRIEVLMNGREGHEGDDDHPRAEHPTPNAPPVVITGTEGMSVQLS
ACCRPIPGDDIMGYIGIGLGMAIHTTDCRVAQRIHRRDPGRWIDVAWAPQPGRLFDVAIKVLVKNTKGVFARVAADITSA
DANIVHIAMDEDQSQESTVLRFVIQVSDRVHLANVMRRVRTNLDVMRIARERPSEEGHRHHDGGMRIDRERADY

Sequences:

>Translated_794_residues
MSTNPSPSATEVDHAAHEHDSDSPSSARKYIDAVLEQSFRHLFGPTATPEQPRRHDVVSIAKLTTALSGYLSPEEIKEVK
AAFHFSDEAHLGQYRQSGEPYITHPVAVAETCAAWKLDAQSIMAALLHDVMEDQGVTKTELAERFGAKVAELVDGLSKLD
KMEFRSREEAQAENFRKMLLAMARDVRVILVKLADRLHNMRTLGAVPPEKRRRVARETLDIYAPIAHRLGLNNTYRELQD
LSFANFNPHRYATLEKAVKAARGNRREVVGKILESVQRAIADAKLDAEVTGREKTIFSIYKKMRDKQLSFSQVLDVYGFR
VVVESPLECYTCLGALHALYKPVPGKFKDYIAIPKVNGYQSLHTTLVGPFGAPIEFQVRTRKMHEIAEAGVAAHWLYKNG
GADLNDVQKRAHQWLKSLLDIQSEVGDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYSIHSDLGNQCVAV
KINNELLPLRTELKSGDIVEVITAPYSKPNPAWLGFVRTGKARTAIRHYLKTMRLNESVQLGERLVDQSLKGYGLALSDV
TPEVWDKLVLWTGNKNRQEIFADIGLGRRVAAVMAKRIEVLMNGREGHEGDDDHPRAEHPTPNAPPVVITGTEGMSVQLS
ACCRPIPGDDIMGYIGIGLGMAIHTTDCRVAQRIHRRDPGRWIDVAWAPQPGRLFDVAIKVLVKNTKGVFARVAADITSA
DANIVHIAMDEDQSQESTVLRFVIQVSDRVHLANVMRRVRTNLDVMRIARERPSEEGHRHHDGGMRIDRERADY
>Mature_793_residues
STNPSPSATEVDHAAHEHDSDSPSSARKYIDAVLEQSFRHLFGPTATPEQPRRHDVVSIAKLTTALSGYLSPEEIKEVKA
AFHFSDEAHLGQYRQSGEPYITHPVAVAETCAAWKLDAQSIMAALLHDVMEDQGVTKTELAERFGAKVAELVDGLSKLDK
MEFRSREEAQAENFRKMLLAMARDVRVILVKLADRLHNMRTLGAVPPEKRRRVARETLDIYAPIAHRLGLNNTYRELQDL
SFANFNPHRYATLEKAVKAARGNRREVVGKILESVQRAIADAKLDAEVTGREKTIFSIYKKMRDKQLSFSQVLDVYGFRV
VVESPLECYTCLGALHALYKPVPGKFKDYIAIPKVNGYQSLHTTLVGPFGAPIEFQVRTRKMHEIAEAGVAAHWLYKNGG
ADLNDVQKRAHQWLKSLLDIQSEVGDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYSIHSDLGNQCVAVK
INNELLPLRTELKSGDIVEVITAPYSKPNPAWLGFVRTGKARTAIRHYLKTMRLNESVQLGERLVDQSLKGYGLALSDVT
PEVWDKLVLWTGNKNRQEIFADIGLGRRVAAVMAKRIEVLMNGREGHEGDDDHPRAEHPTPNAPPVVITGTEGMSVQLSA
CCRPIPGDDIMGYIGIGLGMAIHTTDCRVAQRIHRRDPGRWIDVAWAPQPGRLFDVAIKVLVKNTKGVFARVAADITSAD
ANIVHIAMDEDQSQESTVLRFVIQVSDRVHLANVMRRVRTNLDVMRIARERPSEEGHRHHDGGMRIDRERADY

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=715, Percent_Identity=45.8741258741259, Blast_Score=659, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=684, Percent_Identity=31.4327485380117, Blast_Score=384, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 88765; Mature: 88633

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTNPSPSATEVDHAAHEHDSDSPSSARKYIDAVLEQSFRHLFGPTATPEQPRRHDVVSI
CCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
AKLTTALSGYLSPEEIKEVKAAFHFSDEAHLGQYRQSGEPYITHPVAVAETCAAWKLDAQ
HHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEECCHHHHHHHHHHHCCHH
SIMAALLHDVMEDQGVTKTELAERFGAKVAELVDGLSKLDKMEFRSREEAQAENFRKMLL
HHHHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMARDVRVILVKLADRLHNMRTLGAVPPEKRRRVARETLDIYAPIAHRLGLNNTYRELQD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LSFANFNPHRYATLEKAVKAARGNRREVVGKILESVQRAIADAKLDAEVTGREKTIFSIY
CCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
KKMRDKQLSFSQVLDVYGFRVVVESPLECYTCLGALHALYKPVPGKFKDYIAIPKVNGYQ
HHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHH
SLHTTLVGPFGAPIEFQVRTRKMHEIAEAGVAAHWLYKNGGADLNDVQKRAHQWLKSLLD
HHHHHHCCCCCCCEEEEHHHHHHHHHHHCCHHEEEEEECCCCCHHHHHHHHHHHHHHHHH
IQSEVGDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYSIHSDLGNQCVAV
HHHHCCCHHHHHHHHHHEEECCEEEEECCCHHEEECCCCCHHHHHHHHHHHCCCCCEEEE
KINNELLPLRTELKSGDIVEVITAPYSKPNPAWLGFVRTGKARTAIRHYLKTMRLNESVQ
EECCCEECHHHHCCCCCEEEEEECCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCHHH
LGERLVDQSLKGYGLALSDVTPEVWDKLVLWTGNKNRQEIFADIGLGRRVAAVMAKRIEV
HHHHHHHHHHCCCCEEHHCCCHHHHCCEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHH
LMNGREGHEGDDDHPRAEHPTPNAPPVVITGTEGMSVQLSACCRPIPGDDIMGYIGIGLG
HHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEHHHCCCCCCCCHHHHHHHHHHC
MAIHTTDCRVAQRIHRRDPGRWIDVAWAPQPGRLFDVAIKVLVKNTKGVFARVAADITSA
CEEECCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCC
DANIVHIAMDEDQSQESTVLRFVIQVSDRVHLANVMRRVRTNLDVMRIARERPSEEGHRH
CCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCC
HDGGMRIDRERADY
CCCCCEECHHCCCC
>Mature Secondary Structure 
STNPSPSATEVDHAAHEHDSDSPSSARKYIDAVLEQSFRHLFGPTATPEQPRRHDVVSI
CCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
AKLTTALSGYLSPEEIKEVKAAFHFSDEAHLGQYRQSGEPYITHPVAVAETCAAWKLDAQ
HHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEECCHHHHHHHHHHHCCHH
SIMAALLHDVMEDQGVTKTELAERFGAKVAELVDGLSKLDKMEFRSREEAQAENFRKMLL
HHHHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMARDVRVILVKLADRLHNMRTLGAVPPEKRRRVARETLDIYAPIAHRLGLNNTYRELQD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LSFANFNPHRYATLEKAVKAARGNRREVVGKILESVQRAIADAKLDAEVTGREKTIFSIY
CCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
KKMRDKQLSFSQVLDVYGFRVVVESPLECYTCLGALHALYKPVPGKFKDYIAIPKVNGYQ
HHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHH
SLHTTLVGPFGAPIEFQVRTRKMHEIAEAGVAAHWLYKNGGADLNDVQKRAHQWLKSLLD
HHHHHHCCCCCCCEEEEHHHHHHHHHHHCCHHEEEEEECCCCCHHHHHHHHHHHHHHHHH
IQSEVGDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYSIHSDLGNQCVAV
HHHHCCCHHHHHHHHHHEEECCEEEEECCCHHEEECCCCCHHHHHHHHHHHCCCCCEEEE
KINNELLPLRTELKSGDIVEVITAPYSKPNPAWLGFVRTGKARTAIRHYLKTMRLNESVQ
EECCCEECHHHHCCCCCEEEEEECCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCHHH
LGERLVDQSLKGYGLALSDVTPEVWDKLVLWTGNKNRQEIFADIGLGRRVAAVMAKRIEV
HHHHHHHHHHCCCCEEHHCCCHHHHCCEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHH
LMNGREGHEGDDDHPRAEHPTPNAPPVVITGTEGMSVQLSACCRPIPGDDIMGYIGIGLG
HHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEHHHCCCCCCCCHHHHHHHHHHC
MAIHTTDCRVAQRIHRRDPGRWIDVAWAPQPGRLFDVAIKVLVKNTKGVFARVAADITSA
CEEECCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCC
DANIVHIAMDEDQSQESTVLRFVIQVSDRVHLANVMRRVRTNLDVMRIARERPSEEGHRH
CCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCC
HDGGMRIDRERADY
CCCCCEECHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]