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The map label for this gene is oprM [H]

Identifier: 186475415

GI number: 186475415

Start: 734276

End: 735766

Strand: Direct

Name: oprM [H]

Synonym: Bphy_0649

Alternate gene names: 186475415

Gene position: 734276-735766 (Clockwise)

Preceding gene: 186475414

Following gene: 186475416

Centisome position: 21.1

GC content: 68.01

Gene sequence:

>1491_bases
ATGCGCCGCGCCCTGGTCCTCGGTCCCCTGTGCGCGTTCTGCGCACTGACGCTCAGCGGCTGCCTGCTCGGCCCCGACTA
CTCGCGCCCGCAGGTGGAGGTGCCCGCGACGTACCGCTTTCCCGAGAACTACGCATCGGATGTCGCCAACACCGAATGGT
GGAAGCAGTTCGACGACCCGGTGCTCAACGACCTGATCACCGCGGCGCTGGCGAACAACAACGATGTGAAGATCGCCGCC
GCACGCGTCGACCAGTTCCTCGGCCAGTTCGTCACCACGCGCGCGGCGCTGTTCCCGCAGGTCAGCGCGGGGCTGGATGC
GGCGCGCGAGCGCGTCTCGACCTCGTCGCCGCTGTTCACGGGCGTGCAGAACCCGGTGTTCAACTCGTATCAGCTGGCGC
TGTCGGCGTCGTGGGAGATCGATCTGTTCGGCCACAACCGGCGCCTGACGGAAGCGGCGCGCGCAAGCCTGCTGTCGACG
GAAGAAGCGCGGCGCGGCACGATCCTCACGCTGGTGTCGTCGGTGGCATCGTCGTACATCAACCTGCGCAGCCTCGACCG
GCAGCTGGAGATCGCGAAGGCGACCACGGCGAGCCGCGCCGAATCCGTGCACGTGTTCGAGCTGCGCTTCCAGGGCGGCG
AGGTCTCGCAGATGGAACTCGCGCAGAGCCAGTCCGAATACGAGGACTCGCGCGCGCGCATCCCGCAGATCGAGTCGCAG
ATCGCGCAGCAGGAAGATGCGCTGTCGGTGCTGCTCGGGCGCAATCCGGGCGATATCCTGCGCGGCCGCGCGCTGGGCGA
ACTGGCGGCACCCGCGGTGCCCGCCGGACTGCCGTCGAACCTGCTCGAACGCCGGCCCGATCTGCGTCAGGCCGAGCAGG
ATCTGGTCGCGGCCAATGCGCAGATCGGCGCGGCGAAAGCACTGTATTTCCCGCAGATCTCGATCACGGGGCTGCTCGGC
ACGCAGAGCGGCCAGTTCTCGAGGCTCTTTACGGGGCCGGCGCGCGTGTGGTCGTTTGCCGGCTCCGTGACGCAGCCGAT
CTTCGAGGGCGGCGCGATCGCCGGCCAGGTGAAGCAGGCCGAGGCCGTGCAGCAGCAGGCGCTGTACACGTACCGCAAGG
CGATCCAGGTGGCGTTCCAGGAGGTCGACGATGCGCTGATCTCGTCGCAGAAGCTGCGCGAGCAGTTCGAGGTGCAGGGG
CGGCAGGTGCAGGCGCTCGCGACGTATGCGCATCTGGCGAGGCTGCGGTACGAAGGCGGCTATACGAGCTATATCGAAGT
GCTCGATGCCGAACGCAGCCTGTTCAATGCGCAGCTGAGCGAGACGCAGACGCAGGCGGGCGTGCTGGTGTCGTACGTGA
GCCTGTACAAGGCGATGGGCGGCGGCTGGGTGGTCACGGCTGAGGGCATGACTACCCAGCAGGCCGCGCACGCGGGCGAC
GCGGCACAGCCGGTCAACGGCACGAACGGCACGGTGGAGGAAGTGAAATGA

Upstream 100 bases:

>100_bases
ATAGCGGGCGAGCCGGACAGGGTGCCGCCGTCGGCCGGCGGCCCGGCCGTGGGCGGCGGCCCGAGCACCCGTCCGTCCGC
CCCGCGTGAAGGTGAGTGAC

Downstream 100 bases:

>100_bases
GCGACACGGCCACCTCCGCCTACCTGGGCCACGCGGCGCGCATGCCTGTGATCGCCTGTCACGAATGCGATCTGCTCCAG
AGAGAAAGCACCGTGCCGCC

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MRRALVLGPLCAFCALTLSGCLLGPDYSRPQVEVPATYRFPENYASDVANTEWWKQFDDPVLNDLITAALANNNDVKIAA
ARVDQFLGQFVTTRAALFPQVSAGLDAARERVSTSSPLFTGVQNPVFNSYQLALSASWEIDLFGHNRRLTEAARASLLST
EEARRGTILTLVSSVASSYINLRSLDRQLEIAKATTASRAESVHVFELRFQGGEVSQMELAQSQSEYEDSRARIPQIESQ
IAQQEDALSVLLGRNPGDILRGRALGELAAPAVPAGLPSNLLERRPDLRQAEQDLVAANAQIGAAKALYFPQISITGLLG
TQSGQFSRLFTGPARVWSFAGSVTQPIFEGGAIAGQVKQAEAVQQQALYTYRKAIQVAFQEVDDALISSQKLREQFEVQG
RQVQALATYAHLARLRYEGGYTSYIEVLDAERSLFNAQLSETQTQAGVLVSYVSLYKAMGGGWVVTAEGMTTQQAAHAGD
AAQPVNGTNGTVEEVK

Sequences:

>Translated_496_residues
MRRALVLGPLCAFCALTLSGCLLGPDYSRPQVEVPATYRFPENYASDVANTEWWKQFDDPVLNDLITAALANNNDVKIAA
ARVDQFLGQFVTTRAALFPQVSAGLDAARERVSTSSPLFTGVQNPVFNSYQLALSASWEIDLFGHNRRLTEAARASLLST
EEARRGTILTLVSSVASSYINLRSLDRQLEIAKATTASRAESVHVFELRFQGGEVSQMELAQSQSEYEDSRARIPQIESQ
IAQQEDALSVLLGRNPGDILRGRALGELAAPAVPAGLPSNLLERRPDLRQAEQDLVAANAQIGAAKALYFPQISITGLLG
TQSGQFSRLFTGPARVWSFAGSVTQPIFEGGAIAGQVKQAEAVQQQALYTYRKAIQVAFQEVDDALISSQKLREQFEVQG
RQVQALATYAHLARLRYEGGYTSYIEVLDAERSLFNAQLSETQTQAGVLVSYVSLYKAMGGGWVVTAEGMTTQQAAHAGD
AAQPVNGTNGTVEEVK
>Mature_496_residues
MRRALVLGPLCAFCALTLSGCLLGPDYSRPQVEVPATYRFPENYASDVANTEWWKQFDDPVLNDLITAALANNNDVKIAA
ARVDQFLGQFVTTRAALFPQVSAGLDAARERVSTSSPLFTGVQNPVFNSYQLALSASWEIDLFGHNRRLTEAARASLLST
EEARRGTILTLVSSVASSYINLRSLDRQLEIAKATTASRAESVHVFELRFQGGEVSQMELAQSQSEYEDSRARIPQIESQ
IAQQEDALSVLLGRNPGDILRGRALGELAAPAVPAGLPSNLLERRPDLRQAEQDLVAANAQIGAAKALYFPQISITGLLG
TQSGQFSRLFTGPARVWSFAGSVTQPIFEGGAIAGQVKQAEAVQQQALYTYRKAIQVAFQEVDDALISSQKLREQFEVQG
RQVQALATYAHLARLRYEGGYTSYIEVLDAERSLFNAQLSETQTQAGVLVSYVSLYKAMGGGWVVTAEGMTTQQAAHAGD
AAQPVNGTNGTVEEVK

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=460, Percent_Identity=36.0869565217391, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI2367348, Length=470, Percent_Identity=24.0425531914894, Blast_Score=121, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 53799; Mature: 53799

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRALVLGPLCAFCALTLSGCLLGPDYSRPQVEVPATYRFPENYASDVANTEWWKQFDDP
CCCCEEHHHHHHHHHHHHHCCEECCCCCCCEEECCEEEECCHHHHHHCCCCHHHHHHCCH
VLNDLITAALANNNDVKIAAARVDQFLGQFVTTRAALFPQVSAGLDAARERVSTSSPLFT
HHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
GVQNPVFNSYQLALSASWEIDLFGHNRRLTEAARASLLSTEEARRGTILTLVSSVASSYI
CCCCCCCCCEEEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
NLRSLDRQLEIAKATTASRAESVHVFELRFQGGEVSQMELAQSQSEYEDSRARIPQIESQ
HHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
IAQQEDALSVLLGRNPGDILRGRALGELAAPAVPAGLPSNLLERRPDLRQAEQDLVAANA
HHHHHHHHHHHCCCCCHHHHHCCHHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHHHCC
QIGAAKALYFPQISITGLLGTQSGQFSRLFTGPARVWSFAGSVTQPIFEGGAIAGQVKQA
CCCCHHEEECCCEEEEEEEECCCCCCHHHHCCHHHHHHHHCHHHHHHHCCCCCHHHHHHH
EAVQQQALYTYRKAIQVAFQEVDDALISSQKLREQFEVQGRQVQALATYAHLARLRYEGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
YTSYIEVLDAERSLFNAQLSETQTQAGVLVSYVSLYKAMGGGWVVTAEGMTTQQAAHAGD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCC
AAQPVNGTNGTVEEVK
CCCCCCCCCCCHHCCC
>Mature Secondary Structure
MRRALVLGPLCAFCALTLSGCLLGPDYSRPQVEVPATYRFPENYASDVANTEWWKQFDDP
CCCCEEHHHHHHHHHHHHHCCEECCCCCCCEEECCEEEECCHHHHHHCCCCHHHHHHCCH
VLNDLITAALANNNDVKIAAARVDQFLGQFVTTRAALFPQVSAGLDAARERVSTSSPLFT
HHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
GVQNPVFNSYQLALSASWEIDLFGHNRRLTEAARASLLSTEEARRGTILTLVSSVASSYI
CCCCCCCCCEEEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
NLRSLDRQLEIAKATTASRAESVHVFELRFQGGEVSQMELAQSQSEYEDSRARIPQIESQ
HHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
IAQQEDALSVLLGRNPGDILRGRALGELAAPAVPAGLPSNLLERRPDLRQAEQDLVAANA
HHHHHHHHHHHCCCCCHHHHHCCHHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHHHCC
QIGAAKALYFPQISITGLLGTQSGQFSRLFTGPARVWSFAGSVTQPIFEGGAIAGQVKQA
CCCCHHEEECCCEEEEEEEECCCCCCHHHHCCHHHHHHHHCHHHHHHHCCCCCHHHHHHH
EAVQQQALYTYRKAIQVAFQEVDDALISSQKLREQFEVQGRQVQALATYAHLARLRYEGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
YTSYIEVLDAERSLFNAQLSETQTQAGVLVSYVSLYKAMGGGWVVTAEGMTTQQAAHAGD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCC
AAQPVNGTNGTVEEVK
CCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]