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The map label for this gene is kefA [H]

Identifier: 186475345

GI number: 186475345

Start: 655807

End: 657162

Strand: Direct

Name: kefA [H]

Synonym: Bphy_0578

Alternate gene names: 186475345

Gene position: 655807-657162 (Clockwise)

Preceding gene: 186475344

Following gene: 186475346

Centisome position: 18.85

GC content: 64.53

Gene sequence:

>1356_bases
ATGCAAAACCATCTTTTCTTCCACATGCTGGGCGACGTCGTGCGCGACTTCGGCCAGCCCGTGATTCTCTGGCAGGTGGG
CGTGCTGCTCGGCACGCTGGCGCTCGCCTGGCTGCTTGCGCATCTCGCACGGCACGCGCTCGATGCGCAGCGCGAGACCC
GCTACGGCGCGCTGCGCTTCGGCGCCGAAGCGCTCAATCGCGCGCTATTTCCGCTGCTCGGCGGCGTGCTGGTGTGGATC
GCGCGGGCGATCGCCGGCCAGTTCATTCACACGTCGCTGCTCGATCTGGCGCTGGTGCCGCTGTTTGGCATCGGGCTCAT
CTACATCGTGTTCTATCTCGCGCGGCGTGTGTTCGGGCGGGATGGCAGCGCGCACACGTGGCTTTCGCTGGTCGAAAAGG
TCGTGTCGCTGATCGTGTGGATCGGCATGGTGCTGACCGTGATGGGCATCCAGAACGACGTGCTCAAATGGATGGCGAGC
GTGCAGTTCCGCATCGCCAACACGCATGTGACGCTGCTGTCGCTGCTGTCGGGCTTGCTGTGGGTGTGCGTGACGATGAT
CGTCGCGATGTGGCTCGGCGCGGCCCTGGAAGACCGGCTGATGCGCTCGGGCTCGCTCGACGCGAACCTGAAAGTGGTGC
TGTCGCGCGTCGGTCGCGCGCTGCTGATACTTGGGGCCGTGCTGATCAGCCTGTCGCTGGTCGGTATCGACATCACGGTG
CTCGGCGTGTTCGGCGGGGCGCTCGGCGTGGGCCTCGGTTTCGGCTTGCAGAAGATCGCGAGCAACTACGTGTCGGGCTT
CATCATCCTGATCGACCGGTCGCTGCGCATCGGCGACACGATCAACGTGAGCGGCCTGCAGGGCATGGTCACGCAGATCC
GCACGCGTTATACCGTCGTGCGCGGTCTGGACGGGATCGAAACGCTGATTCCAAACGAAAAGCTGATTACCGACGTCGTG
CAGAACCAGTCGTCGTATCTGACGCGCGGCAACGCGAAGGCGACGGTCCAGATCGCCTATTCGACGGACGTCGAACAGGC
GATGGCGCTGCTGGTCGAGGCCACAGCGGGCGTCGCGCGCGTGCTGCAGGAACCCGCGCCCACGCCTTATCTGGCGGGCT
TCGGCCCCGACGGCATCAATCTCGAACTCGGTTACTGGATCGAAGACGCGGCCACGGGTACGTCCGGTGTGCGCTCGAAT
GTGAACCGCAACATCTGGCGGCTCTTCAGCGAAAACGGCATCGCGATTCCCTATGCTCAACGAGAAGTGCGAATTGTTGC
GGGCGGGAGCGACATCATGCCGCAGGCGGTAAGCGTGACCGGACAGACTGCCAACGACCCGGGCAGCGCCGCGTGA

Upstream 100 bases:

>100_bases
AGCGCCGCGGCTAGAGGTATCCCGCCAATGGCCGGACTTCGCGCGGTCCGGCCGGCGCACGCCGCGTAAGCGGCGCGGCG
CGCCGGCTGCTGGAAATATC

Downstream 100 bases:

>100_bases
ATCGCGGCTTGGCGGGCAAAAAACGCCTGCTTCGACCTCGATGGGGCGTGTGCCCGGTTGATGCAGCACAACAGTTTCCT
TTTTTGTTACAAACACTTGG

Product: mechanosensitive ion channel MscS

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MQNHLFFHMLGDVVRDFGQPVILWQVGVLLGTLALAWLLAHLARHALDAQRETRYGALRFGAEALNRALFPLLGGVLVWI
ARAIAGQFIHTSLLDLALVPLFGIGLIYIVFYLARRVFGRDGSAHTWLSLVEKVVSLIVWIGMVLTVMGIQNDVLKWMAS
VQFRIANTHVTLLSLLSGLLWVCVTMIVAMWLGAALEDRLMRSGSLDANLKVVLSRVGRALLILGAVLISLSLVGIDITV
LGVFGGALGVGLGFGLQKIASNYVSGFIILIDRSLRIGDTINVSGLQGMVTQIRTRYTVVRGLDGIETLIPNEKLITDVV
QNQSSYLTRGNAKATVQIAYSTDVEQAMALLVEATAGVARVLQEPAPTPYLAGFGPDGINLELGYWIEDAATGTSGVRSN
VNRNIWRLFSENGIAIPYAQREVRIVAGGSDIMPQAVSVTGQTANDPGSAA

Sequences:

>Translated_451_residues
MQNHLFFHMLGDVVRDFGQPVILWQVGVLLGTLALAWLLAHLARHALDAQRETRYGALRFGAEALNRALFPLLGGVLVWI
ARAIAGQFIHTSLLDLALVPLFGIGLIYIVFYLARRVFGRDGSAHTWLSLVEKVVSLIVWIGMVLTVMGIQNDVLKWMAS
VQFRIANTHVTLLSLLSGLLWVCVTMIVAMWLGAALEDRLMRSGSLDANLKVVLSRVGRALLILGAVLISLSLVGIDITV
LGVFGGALGVGLGFGLQKIASNYVSGFIILIDRSLRIGDTINVSGLQGMVTQIRTRYTVVRGLDGIETLIPNEKLITDVV
QNQSSYLTRGNAKATVQIAYSTDVEQAMALLVEATAGVARVLQEPAPTPYLAGFGPDGINLELGYWIEDAATGTSGVRSN
VNRNIWRLFSENGIAIPYAQREVRIVAGGSDIMPQAVSVTGQTANDPGSAA
>Mature_451_residues
MQNHLFFHMLGDVVRDFGQPVILWQVGVLLGTLALAWLLAHLARHALDAQRETRYGALRFGAEALNRALFPLLGGVLVWI
ARAIAGQFIHTSLLDLALVPLFGIGLIYIVFYLARRVFGRDGSAHTWLSLVEKVVSLIVWIGMVLTVMGIQNDVLKWMAS
VQFRIANTHVTLLSLLSGLLWVCVTMIVAMWLGAALEDRLMRSGSLDANLKVVLSRVGRALLILGAVLISLSLVGIDITV
LGVFGGALGVGLGFGLQKIASNYVSGFIILIDRSLRIGDTINVSGLQGMVTQIRTRYTVVRGLDGIETLIPNEKLITDVV
QNQSSYLTRGNAKATVQIAYSTDVEQAMALLVEATAGVARVLQEPAPTPYLAGFGPDGINLELGYWIEDAATGTSGVRSN
VNRNIWRLFSENGIAIPYAQREVRIVAGGSDIMPQAVSVTGQTANDPGSAA

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=200, Percent_Identity=35, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI2367355, Length=295, Percent_Identity=28.4745762711864, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1789291, Length=256, Percent_Identity=28.90625, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 48608; Mature: 48608

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CEEEEEECCCCCCCHHHEECCCCCCCCCCCC
>Mature Secondary Structure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CEEEEEECCCCCCCHHHEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]