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The map label for this gene is purN [H]

Identifier: 186475343

GI number: 186475343

Start: 653799

End: 654464

Strand: Direct

Name: purN [H]

Synonym: Bphy_0576

Alternate gene names: 186475343

Gene position: 653799-654464 (Clockwise)

Preceding gene: 186475336

Following gene: 186475344

Centisome position: 18.79

GC content: 66.67

Gene sequence:

>666_bases
ATGAAAAATCTCGTCATTCTGATTTCCGGACGGGGCAGCAACATGGAAGCCATCGTGCGCGCGTGCGCGAGCGAAGGCTG
GCCGGCGCGGGTCGCCGCCGTGATTGCCAACCGTCCCGACGCCGCCGGGCTTGCGTTTGCGGCTTCGCAAGGCATTGCGA
CGGCGGTCGTCGACCACCGCCAGTTTCCTGACCGCGAAAGCTTCGACGCCGCGCTCGCGCGCGAGATCGACGGCTTTGCT
CCCGATCTCGTGGTGCTCGCCGGCTTCATGCGCGTGCTGACCGACGCTTTCGTCAACCGCTACATGGGGCGCATGATCAA
CGTGCATCCGTCGCTGCTGCCGAGCTTTCCGGGCCTCAAGACGCACCAGGCAGCGCTCGACGCGGGCGTGCGTCTGCACG
GCGCGTCCGTGCATTTCGTCACGCCGACGCTCGATCACGGACCTCTCGTGCTGCAATCGGCCGTGCCCGTGCTCGCCGGC
GACGACGCCGCGACGCTCGCCGCGCGCGTCCTCGAAACCGAACATGTCATTTACCCGCGCGCCGTGCGCTGGTTCGTCGA
AGGGCGTCTCGCCGTCGACGGTCTGCGCGTCACCGTGACGCCGCCTGAGCCTCAGTGGCTTTTCCTTGATACAACAAGCA
AGTACAACGCCGGGGAGGGCGCATGA

Upstream 100 bases:

>100_bases
TGAGCGGGCCGGATGCCGCAAAACAATCGATTATAAACGCACGGGGCTCCAGACGGCGCCGCAGCGCGGATTGGGCATGG
CCGAATGATAAAATCCGCGG

Downstream 100 bases:

>100_bases
GACTGCATGGATTTTTGATCGGACAAACCGAGACTTTGCTCGCGGACGTCCTAAAGTTCACCGGTCCCGCCGATGCCGCG
ACCAGCCGCTTCTTCCGCGC

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MKNLVILISGRGSNMEAIVRACASEGWPARVAAVIANRPDAAGLAFAASQGIATAVVDHRQFPDRESFDAALAREIDGFA
PDLVVLAGFMRVLTDAFVNRYMGRMINVHPSLLPSFPGLKTHQAALDAGVRLHGASVHFVTPTLDHGPLVLQSAVPVLAG
DDAATLAARVLETEHVIYPRAVRWFVEGRLAVDGLRVTVTPPEPQWLFLDTTSKYNAGEGA

Sequences:

>Translated_221_residues
MKNLVILISGRGSNMEAIVRACASEGWPARVAAVIANRPDAAGLAFAASQGIATAVVDHRQFPDRESFDAALAREIDGFA
PDLVVLAGFMRVLTDAFVNRYMGRMINVHPSLLPSFPGLKTHQAALDAGVRLHGASVHFVTPTLDHGPLVLQSAVPVLAG
DDAATLAARVLETEHVIYPRAVRWFVEGRLAVDGLRVTVTPPEPQWLFLDTTSKYNAGEGA
>Mature_221_residues
MKNLVILISGRGSNMEAIVRACASEGWPARVAAVIANRPDAAGLAFAASQGIATAVVDHRQFPDRESFDAALAREIDGFA
PDLVVLAGFMRVLTDAFVNRYMGRMINVHPSLLPSFPGLKTHQAALDAGVRLHGASVHFVTPTLDHGPLVLQSAVPVLAG
DDAATLAARVLETEHVIYPRAVRWFVEGRLAVDGLRVTVTPPEPQWLFLDTTSKYNAGEGA

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=185, Percent_Identity=43.2432432432432, Blast_Score=162, Evalue=2e-40,
Organism=Homo sapiens, GI209869995, Length=185, Percent_Identity=43.2432432432432, Blast_Score=162, Evalue=2e-40,
Organism=Homo sapiens, GI209869993, Length=185, Percent_Identity=43.2432432432432, Blast_Score=162, Evalue=2e-40,
Organism=Escherichia coli, GI1788846, Length=208, Percent_Identity=50.4807692307692, Blast_Score=207, Evalue=3e-55,
Organism=Escherichia coli, GI1787483, Length=198, Percent_Identity=30.8080808080808, Blast_Score=98, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17567511, Length=179, Percent_Identity=39.1061452513966, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6320616, Length=200, Percent_Identity=28, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24582400, Length=189, Percent_Identity=42.8571428571429, Blast_Score=164, Evalue=6e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23627; Mature: 23627

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLVILISGRGSNMEAIVRACASEGWPARVAAVIANRPDAAGLAFAASQGIATAVVDHR
CCCEEEEEECCCCCHHHHHHHHHCCCCCHHHHHEECCCCCCCCEEHHHCCCCCEEHHHHC
QFPDRESFDAALAREIDGFAPDLVVLAGFMRVLTDAFVNRYMGRMINVHPSLLPSFPGLK
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHCCCCCCCC
THQAALDAGVRLHGASVHFVTPTLDHGPLVLQSAVPVLAGDDAATLAARVLETEHVIYPR
HHHHHHHCCEEEECCEEEEECCCCCCCCEEEHHCCCEEECCCHHHHHHHHHHHCCEEHHH
AVRWFVEGRLAVDGLRVTVTPPEPQWLFLDTTSKYNAGEGA
HHHHHHCCEEEECCEEEEECCCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MKNLVILISGRGSNMEAIVRACASEGWPARVAAVIANRPDAAGLAFAASQGIATAVVDHR
CCCEEEEEECCCCCHHHHHHHHHCCCCCHHHHHEECCCCCCCCEEHHHCCCCCEEHHHHC
QFPDRESFDAALAREIDGFAPDLVVLAGFMRVLTDAFVNRYMGRMINVHPSLLPSFPGLK
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHCCCCCCCC
THQAALDAGVRLHGASVHFVTPTLDHGPLVLQSAVPVLAGDDAATLAARVLETEHVIYPR
HHHHHHHCCEEEECCEEEEECCCCCCCCEEEHHCCCEEECCCHHHHHHHHHHHCCEEHHH
AVRWFVEGRLAVDGLRVTVTPPEPQWLFLDTTSKYNAGEGA
HHHHHHCCEEEECCEEEEECCCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]