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The map label for this gene is murB

Identifier: 186475296

GI number: 186475296

Start: 599359

End: 600399

Strand: Direct

Name: murB

Synonym: Bphy_0528

Alternate gene names: 186475296

Gene position: 599359-600399 (Clockwise)

Preceding gene: 186475294

Following gene: 186475298

Centisome position: 17.23

GC content: 67.34

Gene sequence:

>1041_bases
ATGTCCCAGCCCGAATCGCTTCCGTTTATCGCCGACTTCCCGCTCAAGCCGCACAACACCTTCGGTTTCGATGTGCGCGC
GAGGCTCGCGTGTCGGATCGAAACCGACGCGCAGCTGCTCGCCGCCTTGCGCGATCCGCGCGCCGCGGGCTTGCGACGGC
TCGTGCTGGGCGGCGGCAGCAACGTCGTGCTGACGGGCGATTTCGACGGACTCGTGTTGCTGGTCGCGCTGCGCGGTCGC
AAGGTCGTTCGCGAAGACGACGAAGCGTGGTACGTCGAAGCGGCTGCGGGCGAGAACTGGCACGAGTTCGTATCGTGGAC
CCTTGCAGAGGGCATGCCCGGTCTCGAGAATCTCGCGCTGATTCCGGGCACGGTGGGCGCGGCGCCGATCCAGAACATCG
GCGCGTACGGGCTGGAGATGTGCGAGCGCTTTGCGTCGCTGCGCGCGGTCGAGCTGGCGACGGGCAAACTCGTCGAACTG
GGCGCGGGCGCTTGCCGCTTCGGCTATCGCGACAGCTTCTTCAAACAGGAAGGCCGCGAGCGCTTCGTCATCGTGTCGGT
GACGTTCAGATTGCCGAAGGTTTGGGCGCCGCGCGCCGGTTACACGGATATCGCGCGCCAACTCGCCGCCGTCGGTCTGG
GCGACGCGACGCCGACACCGCAAGCCATCTTCGATGCCGTCGTCGCCGTGCGGCGCGCCAAGCTGCCGGATCCGTTCGTG
CTCGGCAATGCGGGTAGCTTCTTCAAGAATCCTGTGGTCGAATCGGCGCAGTTCGATGCGCTCGCCGCGAAGGAACCGGA
GATCGTCTCGTATCGGCAGGCGGATGGGCGCGTGAAGCTCGCAGCGGGCTGGCTCATCGACCGTTGCGGCTGGAAAGGGC
GGACGTTGGGCGCGGCGGGCGTGCATGAGCGGCAGGCGCTCGTGCTCGTGAATCGCGGCGGCGCGAGTGGAACCGAGGTG
CTGGCACTGGCGAAGGCGATCCAGCAGGACGTCGCGCAGCGCTTCGGCGTGGAGCTGGAGGCCGAACCCGTCTGTCTGTG
A

Upstream 100 bases:

>100_bases
ACCGGTGTCATTTTACCGACGCCTTTCTCGTTTGCCCAAGACTCGCGAGCGGCTTCGTTCCCGCGTCGCAGTTTCTGCCG
GGCGCTTGCCAGAACATCCG

Downstream 100 bases:

>100_bases
ACGGATTCCGCCGTCGGACAGCGACGCGAGCCTGAAAACAAAAAGCGCCGGGTGTCGAACCCGGCGCTTATGTTTTGCGG
ACGGCGCGCCCGTCCCGGTT

Product: UDP-N-acetylenolpyruvoylglucosamine reductase

Products: NA

Alternate protein names: UDP-N-acetylmuramate dehydrogenase

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MSQPESLPFIADFPLKPHNTFGFDVRARLACRIETDAQLLAALRDPRAAGLRRLVLGGGSNVVLTGDFDGLVLLVALRGR
KVVREDDEAWYVEAAAGENWHEFVSWTLAEGMPGLENLALIPGTVGAAPIQNIGAYGLEMCERFASLRAVELATGKLVEL
GAGACRFGYRDSFFKQEGRERFVIVSVTFRLPKVWAPRAGYTDIARQLAAVGLGDATPTPQAIFDAVVAVRRAKLPDPFV
LGNAGSFFKNPVVESAQFDALAAKEPEIVSYRQADGRVKLAAGWLIDRCGWKGRTLGAAGVHERQALVLVNRGGASGTEV
LALAKAIQQDVAQRFGVELEAEPVCL

Sequences:

>Translated_346_residues
MSQPESLPFIADFPLKPHNTFGFDVRARLACRIETDAQLLAALRDPRAAGLRRLVLGGGSNVVLTGDFDGLVLLVALRGR
KVVREDDEAWYVEAAAGENWHEFVSWTLAEGMPGLENLALIPGTVGAAPIQNIGAYGLEMCERFASLRAVELATGKLVEL
GAGACRFGYRDSFFKQEGRERFVIVSVTFRLPKVWAPRAGYTDIARQLAAVGLGDATPTPQAIFDAVVAVRRAKLPDPFV
LGNAGSFFKNPVVESAQFDALAAKEPEIVSYRQADGRVKLAAGWLIDRCGWKGRTLGAAGVHERQALVLVNRGGASGTEV
LALAKAIQQDVAQRFGVELEAEPVCL
>Mature_345_residues
SQPESLPFIADFPLKPHNTFGFDVRARLACRIETDAQLLAALRDPRAAGLRRLVLGGGSNVVLTGDFDGLVLLVALRGRK
VVREDDEAWYVEAAAGENWHEFVSWTLAEGMPGLENLALIPGTVGAAPIQNIGAYGLEMCERFASLRAVELATGKLVELG
AGACRFGYRDSFFKQEGRERFVIVSVTFRLPKVWAPRAGYTDIARQLAAVGLGDATPTPQAIFDAVVAVRRAKLPDPFVL
GNAGSFFKNPVVESAQFDALAAKEPEIVSYRQADGRVKLAAGWLIDRCGWKGRTLGAAGVHERQALVLVNRGGASGTEVL
ALAKAIQQDVAQRFGVELEAEPVCL

Specific function: Cell wall formation

COG id: COG0812

COG function: function code M; UDP-N-acetylmuramate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain

Homologues:

Organism=Escherichia coli, GI1790407, Length=331, Percent_Identity=51.963746223565, Blast_Score=326, Evalue=1e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURB_BURP8 (B2JDU4)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001856766.1
- ProteinModelPortal:   B2JDU4
- SMR:   B2JDU4
- GeneID:   6242029
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_0528
- HOGENOM:   HBG686573
- OMA:   GAAPMQN
- ProtClustDB:   PRK00046
- GO:   GO:0005737
- HAMAP:   MF_00037
- InterPro:   IPR016169
- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR003170
- InterPro:   IPR011601
- InterPro:   IPR006094
- Gene3D:   G3DSA:3.30.465.10
- Gene3D:   G3DSA:3.30.43.10
- Gene3D:   G3DSA:3.90.78.10
- PANTHER:   PTHR21071
- TIGRFAMs:   TIGR00179

Pfam domain/function: PF01565 FAD_binding_4; PF02873 MurB_C; SSF56176 FAD-binding_2; SSF56194 MurB_C

EC number: =1.1.1.158

Molecular weight: Translated: 37270; Mature: 37139

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS51387 FAD_PCMH

Important sites: ACT_SITE 170-170 ACT_SITE 246-246 ACT_SITE 342-342

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPESLPFIADFPLKPHNTFGFDVRARLACRIETDAQLLAALRDPRAAGLRRLVLGGGS
CCCCCCCCEEEECCCCCCCCCCCEEEEEEEEEECCHHHHHHHHHCCHHHHHEEEEEECCC
NVVLTGDFDGLVLLVALRGRKVVREDDEAWYVEAAAGENWHEFVSWTLAEGMPGLENLAL
CEEEEECCCCEEEEEEHHCCHHEECCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEE
IPGTVGAAPIQNIGAYGLEMCERFASLRAVELATGKLVELGAGACRFGYRDSFFKQEGRE
CCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCEEECCCCCCCCCCCHHHHHHCCCC
RFVIVSVTFRLPKVWAPRAGYTDIARQLAAVGLGDATPTPQAIFDAVVAVRRAKLPDPFV
EEEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEE
LGNAGSFFKNPVVESAQFDALAAKEPEIVSYRQADGRVKLAAGWLIDRCGWKGRTLGAAG
ECCCCCHHCCCCCCCCCCCHHCCCCCCCEEEECCCCEEEEEECHHHHHCCCCCCCCCCCC
VHERQALVLVNRGGASGTEVLALAKAIQQDVAQRFGVELEAEPVCL
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCC
>Mature Secondary Structure 
SQPESLPFIADFPLKPHNTFGFDVRARLACRIETDAQLLAALRDPRAAGLRRLVLGGGS
CCCCCCCEEEECCCCCCCCCCCEEEEEEEEEECCHHHHHHHHHCCHHHHHEEEEEECCC
NVVLTGDFDGLVLLVALRGRKVVREDDEAWYVEAAAGENWHEFVSWTLAEGMPGLENLAL
CEEEEECCCCEEEEEEHHCCHHEECCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEE
IPGTVGAAPIQNIGAYGLEMCERFASLRAVELATGKLVELGAGACRFGYRDSFFKQEGRE
CCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCEEECCCCCCCCCCCHHHHHHCCCC
RFVIVSVTFRLPKVWAPRAGYTDIARQLAAVGLGDATPTPQAIFDAVVAVRRAKLPDPFV
EEEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEE
LGNAGSFFKNPVVESAQFDALAAKEPEIVSYRQADGRVKLAAGWLIDRCGWKGRTLGAAG
ECCCCCHHCCCCCCCCCCCHHCCCCCCCEEEECCCCEEEEEECHHHHHCCCCCCCCCCCC
VHERQALVLVNRGGASGTEVLALAKAIQQDVAQRFGVELEAEPVCL
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA