Click here to switch to the map view.

The map label for this gene is ogt [H]

Identifier: 186475290

GI number: 186475290

Start: 594145

End: 594615

Strand: Direct

Name: ogt [H]

Synonym: Bphy_0522

Alternate gene names: 186475290

Gene position: 594145-594615 (Clockwise)

Preceding gene: 186475289

Following gene: 186475291

Centisome position: 17.08

GC content: 63.69

Gene sequence:

>471_bases
ATGTTCAATGCAGTGATCGACGCGCCGTTCGGCAAGATCGGTGTCCGGCTGGAAAGCGAGACCGTGCGCGAGATCGTCTA
TTTGCCCGATTCGGTGGCGAACGTCGAACCGGATTCGCCGCTCGCAAAGCAGGCCGTCGAGCAGATCGAACACTATCTGC
AACAGGCGTCTGCGCGCTTCGATCTCCCGCTCGCCGACGTCGGCACGCCATTCCAGCGGCGCGTCTGGAAGGCGATCGGC
GAGATTCCGCCGGGCGTCGTGCTGACCTACGGGCAACTCGCGAAGCAGATCGGCAGTGTGCCGCGCGCCGTCGGCCAGGC
GTGCGGCTCGAACTTCTTTCCGATCGTGATCCCGTGTCATCGCGTGGTCGCATCGGGCGGCATCGGCGGCTTTGCGCATA
CGGGCGGCGACGGCTACTATCGCAACGTCAAACGCTGGCTACTGAAACACGAGGGCGTACCTTACGCATGA

Upstream 100 bases:

>100_bases
GCCGGCGATCCTTCGCCGCTGGTGCGCGAGCATGTCGCGTGGGCGCTGGAAGCGGCGTGAGCGCGTTTCGCGTCTGGCGA
AACCGAGAGAGGAGCACATC

Downstream 100 bases:

>100_bases
CTGATGTGCTGACCGAAGACGCGCAGCCCGCGTCGCCTGCTGTTTCGCCGCTGCTGCTGACGAGCGGCGCGTCGATCGAT
GCCTTCTGCGATGCGCTATG

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MFNAVIDAPFGKIGVRLESETVREIVYLPDSVANVEPDSPLAKQAVEQIEHYLQQASARFDLPLADVGTPFQRRVWKAIG
EIPPGVVLTYGQLAKQIGSVPRAVGQACGSNFFPIVIPCHRVVASGGIGGFAHTGGDGYYRNVKRWLLKHEGVPYA

Sequences:

>Translated_156_residues
MFNAVIDAPFGKIGVRLESETVREIVYLPDSVANVEPDSPLAKQAVEQIEHYLQQASARFDLPLADVGTPFQRRVWKAIG
EIPPGVVLTYGQLAKQIGSVPRAVGQACGSNFFPIVIPCHRVVASGGIGGFAHTGGDGYYRNVKRWLLKHEGVPYA
>Mature_156_residues
MFNAVIDAPFGKIGVRLESETVREIVYLPDSVANVEPDSPLAKQAVEQIEHYLQQASARFDLPLADVGTPFQRRVWKAIG
EIPPGVVLTYGQLAKQIGSVPRAVGQACGSNFFPIVIPCHRVVASGGIGGFAHTGGDGYYRNVKRWLLKHEGVPYA

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=172, Percent_Identity=33.7209302325581, Blast_Score=66, Evalue=2e-11,
Organism=Escherichia coli, GI1787596, Length=106, Percent_Identity=49.0566037735849, Blast_Score=86, Evalue=1e-18,
Organism=Escherichia coli, GI1788542, Length=103, Percent_Identity=39.8058252427184, Blast_Score=73, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6320001, Length=93, Percent_Identity=38.7096774193548, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 16929; Mature: 16929

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNAVIDAPFGKIGVRLESETVREIVYLPDSVANVEPDSPLAKQAVEQIEHYLQQASARF
CCCCCCCCCCCHHEEEECHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
DLPLADVGTPFQRRVWKAIGEIPPGVVLTYGQLAKQIGSVPRAVGQACGSNFFPIVIPCH
CCCHHHCCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
RVVASGGIGGFAHTGGDGYYRNVKRWLLKHEGVPYA
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MFNAVIDAPFGKIGVRLESETVREIVYLPDSVANVEPDSPLAKQAVEQIEHYLQQASARF
CCCCCCCCCCCHHEEEECHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
DLPLADVGTPFQRRVWKAIGEIPPGVVLTYGQLAKQIGSVPRAVGQACGSNFFPIVIPCH
CCCHHHCCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
RVVASGGIGGFAHTGGDGYYRNVKRWLLKHEGVPYA
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]