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The map label for this gene is aspS [H]

Identifier: 186475150

GI number: 186475150

Start: 431436

End: 433235

Strand: Direct

Name: aspS [H]

Synonym: Bphy_0381

Alternate gene names: 186475150

Gene position: 431436-433235 (Clockwise)

Preceding gene: 186475149

Following gene: 186475151

Centisome position: 12.4

GC content: 62.28

Gene sequence:

>1800_bases
ATGTCGATGAGATCTGAATACTGCGGTCTGGTGACCGAACACCTGCTGGGTCAAACCGTGTCGCTGTGCGGCTGGGTGAG
CCGGCGCCGCGACCACGGCGGCGTTATCTTCATCGACCTGCGCGACCGCGAAGGTCTCGTTCAGGTTGTCTGCGACCCGG
ACCGCGCAGAGATGTTCAAGACGGCTGAAGGCGTGCGCAACGAGTTCTGCGTGCAGGTGAAGGGCGTCGTGCGCAATCGT
CCCGAAGGTACGACAAACGCCGGTCTGAAGAGCGGCAAGATCGAGGTGCTGTGCCACGAGTTGAACGTGCTGAACGCGTC
GGTGACGCCGCCGTTCCAGCTCGATGACGACAACCTGTCGGAAACCACGCGCCTCACGCATCGGGTGCTGGATCTGCGCC
GTCCGCAGATGCAGCACAACCTGCGCCTGCGCTATCGCGTCGCGATGGAAGTGCGCAAGTATCTTGATTCGCGCGGTTTC
ATCGACATCGAAACGCCAATGCTGACCAAGAGCACGCCGGAAGGCGCGCGCGACTACCTCGTCCCGTCGCGTGTGAACGC
CGGCCAGTTCTTTGCGTTGCCGCAGTCGCCGCAGCTCTTCAAGCAGCTGCTGATGGTCGCGAACTTCGACCGCTACTACC
AGATCACGAAGTGTTTCCGCGACGAAGACCTGCGCGCCGACCGTCAGCCGGAATTCACGCAGATCGACTGCGAAACGTCG
TTCCTGAGCGAGCAGGAAATCCGCGATCTGTTCGAAGACATGACGCGTCACGTGTTCAAGGAGACGATCGGCGTCGAACT
GGATGCGAAGTTCGCCGTGATGCCGTATTCGGAAGCGATGAGCCGCTTCGGCTCGGACAAGCCGGACCTGCGCGTGAAGC
TCGAATTCACGGAACTGACGGACGCGATGAAGGACGTCGACTTCAAGGTGTTCAGCACGCCCGCCAACACGAAGGACGGC
CGCGTCGCGGCGCTGCGCGTGCCGAAGGGCGGCGAGCTTTCGCGCGGCGACATCGACAGCTACACGGAATTCGTCCGCAT
CTACGGCGCGAAGGGTCTCGCGTGGATCAAGATCAACGAAGTGGCGAAGGGCCGTGACGGCCTGCAAAGCCCGATCGTCA
AGAACCTGCACGACGCGGCCATTGCAGCGATCATCGAGCGCACGGGCGCGCAAGACGGCGACATCATCTTCTTCGCGGCG
GATCGCGCGAAGGTCGTGAATGACAGCCTCGGCGCGCTGCGTCTCAAGATCGGCCATTCGGAATTCGGCAAGGCGAACGG
TCTGGTCGAAGCGGGCTGGAAGCCGCTGTGGGTCGTTGACTTCCCGATGTTCGAATACGACGAAGAGGAAAACCGCTACG
TCGCCGCTCATCACCCGTTCACGAGCCCGAAGGACGAGCATCTCGAGTATCTGGAAACGGACCCGGGCCGCTGCCTCGCG
AAGGCTTACGACATCGTGCTTAACGGCTGGGAAATCGGCGGCGGCTCGGTGCGTATCTTCCAGGAAGACGTGCAGAGCAA
GGTGTTCCGCGCGCTGAAGATCGGTGCGGAAGAAGCGCGCCTGAAGTTCGGCTTCCTGCTGGACGCGCTGCAGTACGGTG
CGCCGCCGCACGGCGGTATCGCCTTCGGTCTTGACCGCATTGTCACGATGATGGCCGGAGCAGACTCGATCCGCGATGTG
ATCGCGTTCCCGAAGACGCAGCGAGCGCAAGATCTGCTCACGCAGGCGCCGAGCGAAGTGGACGAGCGTCAGTTGCGCGA
ACTGCACATTCGCCTGCGTCAGCCTGAGCAGAAGGCGTAA

Upstream 100 bases:

>100_bases
CCGCACGCCTGTCGAGCCACCGCTGTAATGCCGGCTCGCGCCGACCACAACCTGGTATGGCGCGCGCCGTTCAACTAATG
CAAAACGAAAGACAAACATC

Downstream 100 bases:

>100_bases
ATGTGACGGGTGCTTCACTCGTCGCCCAAACGGCGGCGAGTGTGCAAAGAACCAACGAAAAAGGCGCGTTATGCGCCTTT
TTCCTTTTTTGCGATAAAGT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 599; Mature: 598

Protein sequence:

>599_residues
MSMRSEYCGLVTEHLLGQTVSLCGWVSRRRDHGGVIFIDLRDREGLVQVVCDPDRAEMFKTAEGVRNEFCVQVKGVVRNR
PEGTTNAGLKSGKIEVLCHELNVLNASVTPPFQLDDDNLSETTRLTHRVLDLRRPQMQHNLRLRYRVAMEVRKYLDSRGF
IDIETPMLTKSTPEGARDYLVPSRVNAGQFFALPQSPQLFKQLLMVANFDRYYQITKCFRDEDLRADRQPEFTQIDCETS
FLSEQEIRDLFEDMTRHVFKETIGVELDAKFAVMPYSEAMSRFGSDKPDLRVKLEFTELTDAMKDVDFKVFSTPANTKDG
RVAALRVPKGGELSRGDIDSYTEFVRIYGAKGLAWIKINEVAKGRDGLQSPIVKNLHDAAIAAIIERTGAQDGDIIFFAA
DRAKVVNDSLGALRLKIGHSEFGKANGLVEAGWKPLWVVDFPMFEYDEEENRYVAAHHPFTSPKDEHLEYLETDPGRCLA
KAYDIVLNGWEIGGGSVRIFQEDVQSKVFRALKIGAEEARLKFGFLLDALQYGAPPHGGIAFGLDRIVTMMAGADSIRDV
IAFPKTQRAQDLLTQAPSEVDERQLRELHIRLRQPEQKA

Sequences:

>Translated_599_residues
MSMRSEYCGLVTEHLLGQTVSLCGWVSRRRDHGGVIFIDLRDREGLVQVVCDPDRAEMFKTAEGVRNEFCVQVKGVVRNR
PEGTTNAGLKSGKIEVLCHELNVLNASVTPPFQLDDDNLSETTRLTHRVLDLRRPQMQHNLRLRYRVAMEVRKYLDSRGF
IDIETPMLTKSTPEGARDYLVPSRVNAGQFFALPQSPQLFKQLLMVANFDRYYQITKCFRDEDLRADRQPEFTQIDCETS
FLSEQEIRDLFEDMTRHVFKETIGVELDAKFAVMPYSEAMSRFGSDKPDLRVKLEFTELTDAMKDVDFKVFSTPANTKDG
RVAALRVPKGGELSRGDIDSYTEFVRIYGAKGLAWIKINEVAKGRDGLQSPIVKNLHDAAIAAIIERTGAQDGDIIFFAA
DRAKVVNDSLGALRLKIGHSEFGKANGLVEAGWKPLWVVDFPMFEYDEEENRYVAAHHPFTSPKDEHLEYLETDPGRCLA
KAYDIVLNGWEIGGGSVRIFQEDVQSKVFRALKIGAEEARLKFGFLLDALQYGAPPHGGIAFGLDRIVTMMAGADSIRDV
IAFPKTQRAQDLLTQAPSEVDERQLRELHIRLRQPEQKA
>Mature_598_residues
SMRSEYCGLVTEHLLGQTVSLCGWVSRRRDHGGVIFIDLRDREGLVQVVCDPDRAEMFKTAEGVRNEFCVQVKGVVRNRP
EGTTNAGLKSGKIEVLCHELNVLNASVTPPFQLDDDNLSETTRLTHRVLDLRRPQMQHNLRLRYRVAMEVRKYLDSRGFI
DIETPMLTKSTPEGARDYLVPSRVNAGQFFALPQSPQLFKQLLMVANFDRYYQITKCFRDEDLRADRQPEFTQIDCETSF
LSEQEIRDLFEDMTRHVFKETIGVELDAKFAVMPYSEAMSRFGSDKPDLRVKLEFTELTDAMKDVDFKVFSTPANTKDGR
VAALRVPKGGELSRGDIDSYTEFVRIYGAKGLAWIKINEVAKGRDGLQSPIVKNLHDAAIAAIIERTGAQDGDIIFFAAD
RAKVVNDSLGALRLKIGHSEFGKANGLVEAGWKPLWVVDFPMFEYDEEENRYVAAHHPFTSPKDEHLEYLETDPGRCLAK
AYDIVLNGWEIGGGSVRIFQEDVQSKVFRALKIGAEEARLKFGFLLDALQYGAPPHGGIAFGLDRIVTMMAGADSIRDVI
AFPKTQRAQDLLTQAPSEVDERQLRELHIRLRQPEQKA

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=610, Percent_Identity=38.5245901639344, Blast_Score=413, Evalue=1e-115,
Organism=Homo sapiens, GI45439306, Length=261, Percent_Identity=29.8850574712644, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI5031815, Length=259, Percent_Identity=23.5521235521236, Blast_Score=76, Evalue=9e-14,
Organism=Homo sapiens, GI194272210, Length=259, Percent_Identity=23.5521235521236, Blast_Score=76, Evalue=9e-14,
Organism=Escherichia coli, GI1788173, Length=591, Percent_Identity=57.1912013536379, Blast_Score=687, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=268, Percent_Identity=25.7462686567164, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1790571, Length=254, Percent_Identity=25.9842519685039, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI32566633, Length=616, Percent_Identity=34.2532467532467, Blast_Score=322, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI17551876, Length=262, Percent_Identity=26.3358778625954, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71994340, Length=258, Percent_Identity=23.2558139534884, Blast_Score=79, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17535927, Length=258, Percent_Identity=23.2558139534884, Blast_Score=79, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17535925, Length=258, Percent_Identity=23.2558139534884, Blast_Score=79, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6325153, Length=637, Percent_Identity=33.7519623233909, Blast_Score=281, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6323011, Length=260, Percent_Identity=29.2307692307692, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6321807, Length=253, Percent_Identity=26.8774703557312, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320242, Length=266, Percent_Identity=26.3157894736842, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324256, Length=261, Percent_Identity=25.6704980842912, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24584738, Length=607, Percent_Identity=36.0790774299835, Blast_Score=349, Evalue=3e-96,
Organism=Drosophila melanogaster, GI17136276, Length=263, Percent_Identity=28.8973384030418, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24640849, Length=256, Percent_Identity=25.390625, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24640851, Length=256, Percent_Identity=25.390625, Blast_Score=74, Evalue=4e-13,
Organism=Drosophila melanogaster, GI19921528, Length=232, Percent_Identity=27.5862068965517, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 67729; Mature: 67598

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMRSEYCGLVTEHLLGQTVSLCGWVSRRRDHGGVIFIDLRDREGLVQVVCDPDRAEMFK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCHHHHHH
TAEGVRNEFCVQVKGVVRNRPEGTTNAGLKSGKIEVLCHELNVLNASVTPPFQLDDDNLS
HHHHHHHHHEEEEEHHHCCCCCCCCCCCCCCCCEEEEEEEHHEEECCCCCCCEECCCCCH
ETTRLTHRVLDLRRPQMQHNLRLRYRVAMEVRKYLDSRGFIDIETPMLTKSTPEGARDYL
HHHHHHHHHHHHCCCHHHCCCEEEHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHCC
VPSRVNAGQFFALPQSPQLFKQLLMVANFDRYYQITKCFRDEDLRADRQPEFTQIDCETS
CCCCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEECCHH
FLSEQEIRDLFEDMTRHVFKETIGVELDAKFAVMPYSEAMSRFGSDKPDLRVKLEFTELT
HCCHHHHHHHHHHHHHHHHHHHHCCEECCEEEECCHHHHHHHHCCCCCCEEEEEEHHHHH
DAMKDVDFKVFSTPANTKDGRVAALRVPKGGELSRGDIDSYTEFVRIYGAKGLAWIKINE
HHHHHCCEEEEECCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECC
VAKGRDGLQSPIVKNLHDAAIAAIIERTGAQDGDIIFFAADRAKVVNDSLGALRLKIGHS
HHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHCCCCCEEEEEECCC
EFGKANGLVEAGWKPLWVVDFPMFEYDEEENRYVAAHHPFTSPKDEHLEYLETDPGRCLA
CCCCCCCEEECCCCEEEEEECCCCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCHHHH
KAYDIVLNGWEIGGGSVRIFQEDVQSKVFRALKIGAEEARLKFGFLLDALQYGAPPHGGI
HHHHHEEECEEECCCEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCH
AFGLDRIVTMMAGADSIRDVIAFPKTQRAQDLLTQAPSEVDERQLRELHIRLRQPEQKA
HHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SMRSEYCGLVTEHLLGQTVSLCGWVSRRRDHGGVIFIDLRDREGLVQVVCDPDRAEMFK
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCHHHHHH
TAEGVRNEFCVQVKGVVRNRPEGTTNAGLKSGKIEVLCHELNVLNASVTPPFQLDDDNLS
HHHHHHHHHEEEEEHHHCCCCCCCCCCCCCCCCEEEEEEEHHEEECCCCCCCEECCCCCH
ETTRLTHRVLDLRRPQMQHNLRLRYRVAMEVRKYLDSRGFIDIETPMLTKSTPEGARDYL
HHHHHHHHHHHHCCCHHHCCCEEEHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHCC
VPSRVNAGQFFALPQSPQLFKQLLMVANFDRYYQITKCFRDEDLRADRQPEFTQIDCETS
CCCCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEECCHH
FLSEQEIRDLFEDMTRHVFKETIGVELDAKFAVMPYSEAMSRFGSDKPDLRVKLEFTELT
HCCHHHHHHHHHHHHHHHHHHHHCCEECCEEEECCHHHHHHHHCCCCCCEEEEEEHHHHH
DAMKDVDFKVFSTPANTKDGRVAALRVPKGGELSRGDIDSYTEFVRIYGAKGLAWIKINE
HHHHHCCEEEEECCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECC
VAKGRDGLQSPIVKNLHDAAIAAIIERTGAQDGDIIFFAADRAKVVNDSLGALRLKIGHS
HHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHCCCCCEEEEEECCC
EFGKANGLVEAGWKPLWVVDFPMFEYDEEENRYVAAHHPFTSPKDEHLEYLETDPGRCLA
CCCCCCCEEECCCCEEEEEECCCCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCHHHH
KAYDIVLNGWEIGGGSVRIFQEDVQSKVFRALKIGAEEARLKFGFLLDALQYGAPPHGGI
HHHHHEEECEEECCCEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCH
AFGLDRIVTMMAGADSIRDVIAFPKTQRAQDLLTQAPSEVDERQLRELHIRLRQPEQKA
HHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA