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The map label for this gene is ubiB

Identifier: 186475146

GI number: 186475146

Start: 427906

End: 429489

Strand: Direct

Name: ubiB

Synonym: Bphy_0377

Alternate gene names: 186475146

Gene position: 427906-429489 (Clockwise)

Preceding gene: 186475145

Following gene: 186475147

Centisome position: 12.3

GC content: 61.3

Gene sequence:

>1584_bases
ATGCGTTTCCTGCGTTTCCTCAAGATTTTCTTTACCGTCATCCGGTTCGGGCTGGACGAGATGATGCTGAGCCGCGTCAA
CGACCGCCGCGTGCGGTTGTTGCTGCGCATTACGACGATCGGCCGGAAATTCGATCAGCCGCCTGGCGTGCGGCTGCGTC
TGGCGCTCGAAAGCCTCGGGCCGATCTTCGTAAAGTTCGGCCAGGTGCTGTCCACACGCCGGGACCTGCTGCGCCCCGAT
ATCGCCACCGAACTCGCGAAGCTGCAAGACCAGGTGCCGCCCTTCGATTCTGCGGTGGCGATCGCCATCATCGAGAAGTC
GCTCGGCGCTCCCGTCGATACGATCTTCGACGACTTCGAGCGTGTGCCCGTGGCGAGCGCGTCGATTGCACAGGTGCATT
TCGCGACCCTGAAGATAGGGCAGCACGCGGGCAAACAGGTCGCCGTGAAGGTGTTGCGGCCGAACATGTTGCCCGTGATC
GATTCCGATCTCGCGTTGCTGCGCGATATCGCCGTGTGGGCCGAGCGCCTGTGGGCGGACGGCAAGCGTCTGAAGCCGCG
CGAAGTGGTGGCGGAATTCGATAAGTATCTCCACGACGAACTCGACCTGATGCGCGAGGCCGCGAACGGCAGCCAGCTGC
GGCGTAACTTCGCGGGGCTCGACCTGTTGCTCGTGCCCGAGATGTATTGGGAATACTGCACGGCGAACGTGCTGGTCATG
GAGCGGATGGTCGGCGTGCCGATCAGCCAGGTCGATACGCTGCGCGTGGCGGGCGTTGATATTCCGAAGCTGGCGCGCGA
GGGTGTCGAGATTTTCTTCACGCAGGTGTTCCGCGACGGATTTTTCCACGCGGACATGCATCCCGGCAATATTCAGGTAA
GTCTCGATCCTGCGCATTTCGGGCGATATATTGCGCTGGATTTCGGCATCATTGGCGCATTGTCGGACTTTGATAAGAAC
TATCTCGCGCAGAACTTTCTGGCGTTCTTCAAGCGCGACTATCACCGTGTCGCCACGTTGCATCTCGAGTCGGGCTGGGT
GCCGGCGACGACGCGCGTCGAAGAACTCGAAAGCGCGATCCGCGCGGTGTGTGAGCCGTATTTCGACCGCGCGCTGAAAG
ACATTTCGCTTGGGCAGGTGCTGATGCGCCTATTCTCGACGTCGCGCCGCTTCAACGTCGAAATCCAGCCGCAGCTCGTG
CTGTTGCAGAAGACAATGCTGAATGTAGAAGGTCTGGGTCGTTCGCTCGATCCGGAACTCGACTTGTGGAAGACGGCGAA
GCCGTATCTGGAGCGTTGGATGAACGAGCAGATCGGCGCAAAAGGCTGGTACGAGCGGCTCAAGATCGAGGCGCCGCAGT
GGAGCAAGACGCTGCCGCAATTGCCGCGCCTGATTCATCACGTGCTGGCGCAGCGTCACGACGCGCAGCAGCGCGGCATC
AACGACGAAACCATCCGCCAGATCCTGCTCGAACAGAAGCGCACGAACCGGCTGCTGCAAGGGCTATTGATGTTCGGTGT
AGCAGTGGGCGTGGGCGCGGTGCTGGCGCGCGCGTGGCTCGCCATCGCGTACGGCGGCTATTGA

Upstream 100 bases:

>100_bases
ACGCATCGAGCGTCTGGAACAGAAGGTCGTAGCCCGCGGCGCCGATGCGCCCGGCGCCGCCGCCATGTCGCGCGGCACGC
GCTAGTCACCGGGCTTAACC

Downstream 100 bases:

>100_bases
GCGCATTCCACGAGGGTGTTCCATGAGTGACAAGAGGCCGTCCGTTCCCCCTTCGGCGCCGGACTTCGAAAACCGCGATC
CGAATGCGCCCGGCTTTTGG

Product: putative ubiquinone biosynthesis protein UbiB

Products: 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: NA

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MRFLRFLKIFFTVIRFGLDEMMLSRVNDRRVRLLLRITTIGRKFDQPPGVRLRLALESLGPIFVKFGQVLSTRRDLLRPD
IATELAKLQDQVPPFDSAVAIAIIEKSLGAPVDTIFDDFERVPVASASIAQVHFATLKIGQHAGKQVAVKVLRPNMLPVI
DSDLALLRDIAVWAERLWADGKRLKPREVVAEFDKYLHDELDLMREAANGSQLRRNFAGLDLLLVPEMYWEYCTANVLVM
ERMVGVPISQVDTLRVAGVDIPKLAREGVEIFFTQVFRDGFFHADMHPGNIQVSLDPAHFGRYIALDFGIIGALSDFDKN
YLAQNFLAFFKRDYHRVATLHLESGWVPATTRVEELESAIRAVCEPYFDRALKDISLGQVLMRLFSTSRRFNVEIQPQLV
LLQKTMLNVEGLGRSLDPELDLWKTAKPYLERWMNEQIGAKGWYERLKIEAPQWSKTLPQLPRLIHHVLAQRHDAQQRGI
NDETIRQILLEQKRTNRLLQGLLMFGVAVGVGAVLARAWLAIAYGGY

Sequences:

>Translated_527_residues
MRFLRFLKIFFTVIRFGLDEMMLSRVNDRRVRLLLRITTIGRKFDQPPGVRLRLALESLGPIFVKFGQVLSTRRDLLRPD
IATELAKLQDQVPPFDSAVAIAIIEKSLGAPVDTIFDDFERVPVASASIAQVHFATLKIGQHAGKQVAVKVLRPNMLPVI
DSDLALLRDIAVWAERLWADGKRLKPREVVAEFDKYLHDELDLMREAANGSQLRRNFAGLDLLLVPEMYWEYCTANVLVM
ERMVGVPISQVDTLRVAGVDIPKLAREGVEIFFTQVFRDGFFHADMHPGNIQVSLDPAHFGRYIALDFGIIGALSDFDKN
YLAQNFLAFFKRDYHRVATLHLESGWVPATTRVEELESAIRAVCEPYFDRALKDISLGQVLMRLFSTSRRFNVEIQPQLV
LLQKTMLNVEGLGRSLDPELDLWKTAKPYLERWMNEQIGAKGWYERLKIEAPQWSKTLPQLPRLIHHVLAQRHDAQQRGI
NDETIRQILLEQKRTNRLLQGLLMFGVAVGVGAVLARAWLAIAYGGY
>Mature_527_residues
MRFLRFLKIFFTVIRFGLDEMMLSRVNDRRVRLLLRITTIGRKFDQPPGVRLRLALESLGPIFVKFGQVLSTRRDLLRPD
IATELAKLQDQVPPFDSAVAIAIIEKSLGAPVDTIFDDFERVPVASASIAQVHFATLKIGQHAGKQVAVKVLRPNMLPVI
DSDLALLRDIAVWAERLWADGKRLKPREVVAEFDKYLHDELDLMREAANGSQLRRNFAGLDLLLVPEMYWEYCTANVLVM
ERMVGVPISQVDTLRVAGVDIPKLAREGVEIFFTQVFRDGFFHADMHPGNIQVSLDPAHFGRYIALDFGIIGALSDFDKN
YLAQNFLAFFKRDYHRVATLHLESGWVPATTRVEELESAIRAVCEPYFDRALKDISLGQVLMRLFSTSRRFNVEIQPQLV
LLQKTMLNVEGLGRSLDPELDLWKTAKPYLERWMNEQIGAKGWYERLKIEAPQWSKTLPQLPRLIHHVLAQRHDAQQRGI
NDETIRQILLEQKRTNRLLQGLLMFGVAVGVGAVLARAWLAIAYGGY

Specific function: Required, probably indirectly, for the hydroxylation of 2-octaprenylphenol to 2-octaprenyl-6-hydroxy-phenol, the fourth step in ubiquinone biosynthesis

COG id: COG0661

COG function: function code R; Predicted unusual protein kinase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ABC1 family. UbiB subfamily

Homologues:

Organism=Homo sapiens, GI40254938, Length=262, Percent_Identity=29.3893129770992, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI41393593, Length=276, Percent_Identity=28.6231884057971, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI32261307, Length=389, Percent_Identity=26.9922879177378, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI217035081, Length=216, Percent_Identity=29.6296296296296, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI34147522, Length=192, Percent_Identity=28.6458333333333, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI2367309, Length=526, Percent_Identity=49.8098859315589, Blast_Score=498, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI17559152, Length=280, Percent_Identity=28.5714285714286, Blast_Score=119, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6325148, Length=451, Percent_Identity=25.4988913525499, Blast_Score=121, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6323282, Length=255, Percent_Identity=26.2745098039216, Blast_Score=87, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6321319, Length=217, Percent_Identity=29.9539170506912, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI22024280, Length=272, Percent_Identity=30.5147058823529, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24662575, Length=260, Percent_Identity=30.7692307692308, Blast_Score=108, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UBIB_BURP8 (B2JCV1)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001856616.1
- ProteinModelPortal:   B2JCV1
- GeneID:   6241879
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_0377
- HOGENOM:   HBG593509
- OMA:   RIQRVVI
- ProtClustDB:   PRK04750
- HAMAP:   MF_00414
- InterPro:   IPR004147
- InterPro:   IPR011009
- InterPro:   IPR010232
- TIGRFAMs:   TIGR01982

Pfam domain/function: PF03109 ABC1; SSF56112 Kinase_like

EC number: NA

Molecular weight: Translated: 60105; Mature: 60105

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1ba6240c)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFLRFLKIFFTVIRFGLDEMMLSRVNDRRVRLLLRITTIGRKFDQPPGVRLRLALESLG
CHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHC
PIFVKFGQVLSTRRDLLRPDIATELAKLQDQVPPFDSAVAIAIIEKSLGAPVDTIFDDFE
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHH
RVPVASASIAQVHFATLKIGQHAGKQVAVKVLRPNMLPVIDSDLALLRDIAVWAERLWAD
HCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCC
GKRLKPREVVAEFDKYLHDELDLMREAANGSQLRRNFAGLDLLLVPEMYWEYCTANVLVM
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHH
ERMVGVPISQVDTLRVAGVDIPKLAREGVEIFFTQVFRDGFFHADMHPGNIQVSLDPAHF
HHHHCCCCHHCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEEECHHHH
GRYIALDFGIIGALSDFDKNYLAQNFLAFFKRDYHRVATLHLESGWVPATTRVEELESAI
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHH
RAVCEPYFDRALKDISLGQVLMRLFSTSRRFNVEIQPQLVLLQKTMLNVEGLGRSLDPEL
HHHHHHHHHHHHHHCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCC
DLWKTAKPYLERWMNEQIGAKGWYERLKIEAPQWSKTLPQLPRLIHHVLAQRHDAQQRGI
HHHHCHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCC
NDETIRQILLEQKRTNRLLQGLLMFGVAVGVGAVLARAWLAIAYGGY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
>Mature Secondary Structure
MRFLRFLKIFFTVIRFGLDEMMLSRVNDRRVRLLLRITTIGRKFDQPPGVRLRLALESLG
CHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHC
PIFVKFGQVLSTRRDLLRPDIATELAKLQDQVPPFDSAVAIAIIEKSLGAPVDTIFDDFE
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHH
RVPVASASIAQVHFATLKIGQHAGKQVAVKVLRPNMLPVIDSDLALLRDIAVWAERLWAD
HCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCC
GKRLKPREVVAEFDKYLHDELDLMREAANGSQLRRNFAGLDLLLVPEMYWEYCTANVLVM
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHH
ERMVGVPISQVDTLRVAGVDIPKLAREGVEIFFTQVFRDGFFHADMHPGNIQVSLDPAHF
HHHHCCCCHHCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEEECHHHH
GRYIALDFGIIGALSDFDKNYLAQNFLAFFKRDYHRVATLHLESGWVPATTRVEELESAI
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHH
RAVCEPYFDRALKDISLGQVLMRLFSTSRRFNVEIQPQLVLLQKTMLNVEGLGRSLDPEL
HHHHHHHHHHHHHHCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCC
DLWKTAKPYLERWMNEQIGAKGWYERLKIEAPQWSKTLPQLPRLIHHVLAQRHDAQQRGI
HHHHCHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCC
NDETIRQILLEQKRTNRLLQGLLMFGVAVGVGAVLARAWLAIAYGGY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2 2-octaprenylphenol; O2 [C]

Specific reaction: 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA