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The map label for this gene is ubiE

Identifier: 186475143

GI number: 186475143

Start: 425374

End: 426105

Strand: Direct

Name: ubiE

Synonym: Bphy_0374

Alternate gene names: 186475143

Gene position: 425374-426105 (Clockwise)

Preceding gene: 186475142

Following gene: 186475144

Centisome position: 12.23

GC content: 59.02

Gene sequence:

>732_bases
ATGACCCAAACCCACTTCGGCTATCAGACAGTCGACGAACAGGAAAAAGCGAAGAAAGTGGCCGGCGTATTCCACTCGGT
CGCCTCCAACTATGACCTGATGAACGACCTGATGTCGGGCGGGCTGCACCGCGCGTGGAAGGCGTTCACGATCGCGCAGG
CCAACGTGCGGCCGGGATACAAGGTGCTCGACATCGCGGGCGGCACGGGCGACCTGTCGAAGGCGTTCGCCAAGCGCGCG
GGCGAGACGGGGGAAGTCTGGCATACGGACATCAACGAATCGATGCTGCGCGTGGGCCGCGACCGTTTGATCGACAAGGG
TGTCATCACGCCGACGCTGCTTTGCGATGCCGAAAAGATTCCGTTTCCGGACAACTACTTCGATGTGGTCACGGTGGCAT
TCGGCTTGCGCAACATGACGCACAAGGATGCCGCGCTCGCCGAAATGCGCCGCGTGCTCAAACCGGCCGGGCGTCTGCTC
GTGCTGGAATTCTCAAAGGTCTGGGATCCACTCAAAAAAGCCTACGACGTCTATTCTTTCAAGGTGCTCCCGTGGCTGGG
CGACCGCTTTGCCAAGGACGCGGACAGCTATCGCTACCTCGCGGAATCGATCCGGATGCATCCGGACCAGGAAACTTTGA
AGACGATGATGGAACAAGCTGGCCTCGACGCCGTCAAATATTACAATTTGTCAGCTGGCGTGGTAGCCTTGCATGTGGGG
ACGAAATACTGA

Upstream 100 bases:

>100_bases
ATCCAGTCCAGCGCACGGCCATAGACACTGATACGATTCGGCGGCGCGCCGCTCAGGTGCGACGCAACAATTTTCAGAAT
ATGCGAAAGGACGAAGCGCG

Downstream 100 bases:

>100_bases
TGTCCTAACCTCCCGTTTTTAAAGGAAATTGAGAAAATGTCCGATTCGAATATGTCATCTCGTTGTAAGGTTAAGGGGTC
GTGGGCGAGGAGAATCGGAC

Product: ubiquinone/menaquinone biosynthesis methyltransferase

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MTQTHFGYQTVDEQEKAKKVAGVFHSVASNYDLMNDLMSGGLHRAWKAFTIAQANVRPGYKVLDIAGGTGDLSKAFAKRA
GETGEVWHTDINESMLRVGRDRLIDKGVITPTLLCDAEKIPFPDNYFDVVTVAFGLRNMTHKDAALAEMRRVLKPAGRLL
VLEFSKVWDPLKKAYDVYSFKVLPWLGDRFAKDADSYRYLAESIRMHPDQETLKTMMEQAGLDAVKYYNLSAGVVALHVG
TKY

Sequences:

>Translated_243_residues
MTQTHFGYQTVDEQEKAKKVAGVFHSVASNYDLMNDLMSGGLHRAWKAFTIAQANVRPGYKVLDIAGGTGDLSKAFAKRA
GETGEVWHTDINESMLRVGRDRLIDKGVITPTLLCDAEKIPFPDNYFDVVTVAFGLRNMTHKDAALAEMRRVLKPAGRLL
VLEFSKVWDPLKKAYDVYSFKVLPWLGDRFAKDADSYRYLAESIRMHPDQETLKTMMEQAGLDAVKYYNLSAGVVALHVG
TKY
>Mature_242_residues
TQTHFGYQTVDEQEKAKKVAGVFHSVASNYDLMNDLMSGGLHRAWKAFTIAQANVRPGYKVLDIAGGTGDLSKAFAKRAG
ETGEVWHTDINESMLRVGRDRLIDKGVITPTLLCDAEKIPFPDNYFDVVTVAFGLRNMTHKDAALAEMRRVLKPAGRLLV
LEFSKVWDPLKKAYDVYSFKVLPWLGDRFAKDADSYRYLAESIRMHPDQETLKTMMEQAGLDAVKYYNLSAGVVALHVGT
KY

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2)

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family

Homologues:

Organism=Homo sapiens, GI116063536, Length=277, Percent_Identity=44.043321299639, Blast_Score=223, Evalue=1e-58,
Organism=Escherichia coli, GI48994983, Length=243, Percent_Identity=63.7860082304527, Blast_Score=313, Evalue=6e-87,
Organism=Caenorhabditis elegans, GI17557039, Length=244, Percent_Identity=45.0819672131148, Blast_Score=213, Evalue=8e-56,
Organism=Saccharomyces cerevisiae, GI6323526, Length=257, Percent_Identity=42.0233463035019, Blast_Score=170, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24641702, Length=255, Percent_Identity=45.0980392156863, Blast_Score=218, Evalue=4e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UBIE_BURP8 (B2JCU8)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001856613.1
- ProteinModelPortal:   B2JCU8
- SMR:   B2JCU8
- GeneID:   6241876
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_0374
- HOGENOM:   HBG559580
- OMA:   FDVYTIA
- ProtClustDB:   PRK00216
- HAMAP:   MF_01813
- InterPro:   IPR004033
- PANTHER:   PTHR10108:SF24
- TIGRFAMs:   TIGR01934

Pfam domain/function: PF01209 Ubie_methyltran

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27258; Mature: 27126

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: PS01183 UBIE_1; PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTHFGYQTVDEQEKAKKVAGVFHSVASNYDLMNDLMSGGLHRAWKAFTIAQANVRPGY
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHEEECCCCCCC
KVLDIAGGTGDLSKAFAKRAGETGEVWHTDINESMLRVGRDRLIDKGVITPTLLCDAEKI
EEEEECCCCCHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCHHHEECCCCC
PFPDNYFDVVTVAFGLRNMTHKDAALAEMRRVLKPAGRLLVLEFSKVWDPLKKAYDVYSF
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHE
KVLPWLGDRFAKDADSYRYLAESIRMHPDQETLKTMMEQAGLDAVKYYNLSAGVVALHVG
EEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHEEECCCCCEEEEECC
TKY
CCC
>Mature Secondary Structure 
TQTHFGYQTVDEQEKAKKVAGVFHSVASNYDLMNDLMSGGLHRAWKAFTIAQANVRPGY
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHEEECCCCCCC
KVLDIAGGTGDLSKAFAKRAGETGEVWHTDINESMLRVGRDRLIDKGVITPTLLCDAEKI
EEEEECCCCCHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCHHHEECCCCC
PFPDNYFDVVTVAFGLRNMTHKDAALAEMRRVLKPAGRLLVLEFSKVWDPLKKAYDVYSF
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHE
KVLPWLGDRFAKDADSYRYLAESIRMHPDQETLKTMMEQAGLDAVKYYNLSAGVVALHVG
EEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHEEECCCCCEEEEECC
TKY
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA