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The map label for this gene is nagE [H]

Identifier: 186475065

GI number: 186475065

Start: 338304

End: 340070

Strand: Direct

Name: nagE [H]

Synonym: Bphy_0296

Alternate gene names: 186475065

Gene position: 338304-340070 (Clockwise)

Preceding gene: 186475064

Following gene: 186475071

Centisome position: 9.72

GC content: 64.97

Gene sequence:

>1767_bases
ATGGATGGGAATCCGTTTCTGAAGGTTCAGCGCCTTGGCCGCGCACTGATGCTGCCGATCGCGGTGTTGCCGGTAGCCGG
CCTGTTGCTGCGCCTCGGTCAGCCCGATGTGTTCAACATCAAGATGATCGCCGACGCGGGCGGCGCGATCTTCGACAACC
TGCCGCTTCTGTTCGCGATCGGCGTCGCTGTCGGTTTCGCGAAGGATAACAACGGCGTGGCAGCGCTGGCGGGCGCGATC
GGCTATCTGATCGAAGTCGCCGTGATGAAGGACATCAACGACAAGCTCAACATGGGCGTGCTGTCGGGGATCATCGCCGG
TATCGTCGCGGGGATGCTGTACAACCGGTACAAGGACATCAAGCTACCCGACTACCTCGCGTTCTTCGGCGGCAAGCGCT
TCGTGCCGATTGTCACGGGGGTCGCGTGTCTGGTGTTGGGCATCGTGTTCGGCTACGCGTGGGCGCCCGTTCAGGCCGCC
ATCGACGCCGCCGGCCACTGGCTGACGACGGCAGGCGCGGTCGGTGCATTCGTGTTCGGCCTGCTGAACCGGCTGCTGCT
CGTGACGGGTCTGCATCACATCCTGAACTCGCTCGCATGGTTCGTGTTCGGCACATTCACGCCGCCGGGCGGCGCGGCCG
TGACAGGCGACCTGCATCGCTTCTTTGCAGGCGACCCGACCGCGGGCACTTTCATGACGGGCTTCTTCCCGATCATGATG
TTCGGTCTGCCCGCAGCGTGTCTCGCAATGCTCCACGAAGCGCCCAAGGAGCGCCGCGCACTGGTCGGCGGCCTGCTGTT
CTCGATGGCGCTGACGTCGTTCCTGACGGGCGTGACGGAGCCGATCGAGTTCAGCTTCATGTTCCTCGCGCCAGTGCTGT
ACGCGATCCACGCGGTGCTGACGGGCCTCTCGCTCGCAATCTGTTCGGCGCTCGGCATCAAGCTCGGCTTCACGTTCTCG
GCGGGCGCGATCGACTACGTGCTCAACTACGGTCTATCGACAAAAGGCTGGGAAGCGATCCCCATCGGGATTGCCTATTC
GGTCGTCTACTACGGCCTGTTCCGCTTCTTCATCCGCAAGTTCAACATGGCGACGCCGGGCCGCGAGCAGGAAACGCCGG
ACGTGGCCAACGAATCGTTCTCTGCGGATGGCTTCGTCGCGCCTGTCGGGGGCTCAGCCGCAACGTCGGGCACGCGAGCG
CAGCGATACATCGCGGCGCTCGGCGGTGCAGCGAATCTGTCGGTGGTCGATGCATGCACGACGCGTCTGCGTCTGTCGGT
GGTGGACTCGGAGAAGGTGTCGGACAACGAGCTGAAGTCGATCGGTGCTCGCGGCGTGCTGAAGCGCGGCGGCGGCAACG
TACAGGTGATCATCGGACCGGAAGCGGACATCATCGCCGATGAAATCCGCACGACGATTGCGAGCGGCGGCGCGGCTGCT
GCGTCGCCCGCGAAGCCCGCTGTTGCGTCGGCCCCTGTCGTAACCGCTGCAACGGCTGCAACGGCGGCAGCTGGCAACGG
CCCGCTCGATCCGGACCCGCTGCGCTGGCTCGCTGTGTTCGGCGGCGCGACGAACGTCGTGTCGCTGGAAGCGGTGGCGG
CGACACGCTTGCGTATCGTCGTGCGCGACGCGTCGGCGATCGATCGTCAGCGTCTCGACACGCTGAATGTAGCGTGGGTG
TCGACGGATACGTTCCACATCGTCGTCGGCGACGCGGCGCCGCGTTACGCGCAGCAACTGTCGACGCGTCTGACAGCAAC
GGCATGA

Upstream 100 bases:

>100_bases
GCGTCGCAACCAGTCCGAACGACAGATTCGTCAGCTCGATATTTCAACCAGCAAGTCAAGCAAGTACGCAACTACACGAG
ACAAAGGAGTGGAGGTATCA

Downstream 100 bases:

>100_bases
GTTTCGTGAAGGCCTGAGTCTTCGTCGCTAAAGAGCCGGCGCCCTCGAGGCGCCGGTTTCTATTTGCGGCTCAGCGTTCG
TCGCGGGCCGCCTGGGCATC

Product: PTS system N-acetylglucosamine-specific transporter subunit IIBC

Products: NA

Alternate protein names: EIICBA-Nag; EII-Nag; N-acetylglucosamine permease IIC component; PTS system N-acetylglucosamine-specific EIIC component; N-acetylglucosamine-specific phosphotransferase enzyme IIB component; PTS system N-acetylglucosamine-specific EIIB component; N-acetylglucosamine-specific phosphotransferase enzyme IIA component; PTS system N-acetylglucosamine-specific EIIA component [H]

Number of amino acids: Translated: 588; Mature: 588

Protein sequence:

>588_residues
MDGNPFLKVQRLGRALMLPIAVLPVAGLLLRLGQPDVFNIKMIADAGGAIFDNLPLLFAIGVAVGFAKDNNGVAALAGAI
GYLIEVAVMKDINDKLNMGVLSGIIAGIVAGMLYNRYKDIKLPDYLAFFGGKRFVPIVTGVACLVLGIVFGYAWAPVQAA
IDAAGHWLTTAGAVGAFVFGLLNRLLLVTGLHHILNSLAWFVFGTFTPPGGAAVTGDLHRFFAGDPTAGTFMTGFFPIMM
FGLPAACLAMLHEAPKERRALVGGLLFSMALTSFLTGVTEPIEFSFMFLAPVLYAIHAVLTGLSLAICSALGIKLGFTFS
AGAIDYVLNYGLSTKGWEAIPIGIAYSVVYYGLFRFFIRKFNMATPGREQETPDVANESFSADGFVAPVGGSAATSGTRA
QRYIAALGGAANLSVVDACTTRLRLSVVDSEKVSDNELKSIGARGVLKRGGGNVQVIIGPEADIIADEIRTTIASGGAAA
ASPAKPAVASAPVVTAATAATAAAGNGPLDPDPLRWLAVFGGATNVVSLEAVAATRLRIVVRDASAIDRQRLDTLNVAWV
STDTFHIVVGDAAPRYAQQLSTRLTATA

Sequences:

>Translated_588_residues
MDGNPFLKVQRLGRALMLPIAVLPVAGLLLRLGQPDVFNIKMIADAGGAIFDNLPLLFAIGVAVGFAKDNNGVAALAGAI
GYLIEVAVMKDINDKLNMGVLSGIIAGIVAGMLYNRYKDIKLPDYLAFFGGKRFVPIVTGVACLVLGIVFGYAWAPVQAA
IDAAGHWLTTAGAVGAFVFGLLNRLLLVTGLHHILNSLAWFVFGTFTPPGGAAVTGDLHRFFAGDPTAGTFMTGFFPIMM
FGLPAACLAMLHEAPKERRALVGGLLFSMALTSFLTGVTEPIEFSFMFLAPVLYAIHAVLTGLSLAICSALGIKLGFTFS
AGAIDYVLNYGLSTKGWEAIPIGIAYSVVYYGLFRFFIRKFNMATPGREQETPDVANESFSADGFVAPVGGSAATSGTRA
QRYIAALGGAANLSVVDACTTRLRLSVVDSEKVSDNELKSIGARGVLKRGGGNVQVIIGPEADIIADEIRTTIASGGAAA
ASPAKPAVASAPVVTAATAATAAAGNGPLDPDPLRWLAVFGGATNVVSLEAVAATRLRIVVRDASAIDRQRLDTLNVAWV
STDTFHIVVGDAAPRYAQQLSTRLTATA
>Mature_588_residues
MDGNPFLKVQRLGRALMLPIAVLPVAGLLLRLGQPDVFNIKMIADAGGAIFDNLPLLFAIGVAVGFAKDNNGVAALAGAI
GYLIEVAVMKDINDKLNMGVLSGIIAGIVAGMLYNRYKDIKLPDYLAFFGGKRFVPIVTGVACLVLGIVFGYAWAPVQAA
IDAAGHWLTTAGAVGAFVFGLLNRLLLVTGLHHILNSLAWFVFGTFTPPGGAAVTGDLHRFFAGDPTAGTFMTGFFPIMM
FGLPAACLAMLHEAPKERRALVGGLLFSMALTSFLTGVTEPIEFSFMFLAPVLYAIHAVLTGLSLAICSALGIKLGFTFS
AGAIDYVLNYGLSTKGWEAIPIGIAYSVVYYGLFRFFIRKFNMATPGREQETPDVANESFSADGFVAPVGGSAATSGTRA
QRYIAALGGAANLSVVDACTTRLRLSVVDSEKVSDNELKSIGARGVLKRGGGNVQVIIGPEADIIADEIRTTIASGGAAA
ASPAKPAVASAPVVTAATAATAAAGNGPLDPDPLRWLAVFGGATNVVSLEAVAATRLRIVVRDASAIDRQRLDTLNVAWV
STDTFHIVVGDAAPRYAQQLSTRLTATA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=470, Percent_Identity=55.7446808510638, Blast_Score=489, Evalue=1e-139,
Organism=Escherichia coli, GI1787343, Length=494, Percent_Identity=43.7246963562753, Blast_Score=380, Evalue=1e-107,
Organism=Escherichia coli, GI1787908, Length=517, Percent_Identity=37.7176015473888, Blast_Score=285, Evalue=7e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010974 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 61315; Mature: 61315

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGNPFLKVQRLGRALMLPIAVLPVAGLLLRLGQPDVFNIKMIADAGGAIFDNLPLLFAI
CCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHCCHHHHHH
GVAVGFAKDNNGVAALAGAIGYLIEVAVMKDINDKLNMGVLSGIIAGIVAGMLYNRYKDI
HHHHEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
KLPDYLAFFGGKRFVPIVTGVACLVLGIVFGYAWAPVQAAIDAAGHWLTTAGAVGAFVFG
CCCHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LLNRLLLVTGLHHILNSLAWFVFGTFTPPGGAAVTGDLHRFFAGDPTAGTFMTGFFPIMM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECHHHHHHCCCCCCCHHHHHHHHHHH
FGLPAACLAMLHEAPKERRALVGGLLFSMALTSFLTGVTEPIEFSFMFLAPVLYAIHAVL
HCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
TGLSLAICSALGIKLGFTFSAGAIDYVLNYGLSTKGWEAIPIGIAYSVVYYGLFRFFIRK
HHHHHHHHHHHHHHCCEEECCHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHH
FNMATPGREQETPDVANESFSADGFVAPVGGSAATSGTRAQRYIAALGGAANLSVVDACT
HCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHH
TRLRLSVVDSEKVSDNELKSIGARGVLKRGGGNVQVIIGPEADIIADEIRTTIASGGAAA
HHEEEEEECCCCCCHHHHHHHCCCHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCC
ASPAKPAVASAPVVTAATAATAAAGNGPLDPDPLRWLAVFGGATNVVSLEAVAATRLRIV
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCEEEHHHHHHHEEEEE
VRDASAIDRQRLDTLNVAWVSTDTFHIVVGDAAPRYAQQLSTRLTATA
EECHHHHHHHHCCEEEEEEEECCEEEEEECCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDGNPFLKVQRLGRALMLPIAVLPVAGLLLRLGQPDVFNIKMIADAGGAIFDNLPLLFAI
CCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHCCHHHHHH
GVAVGFAKDNNGVAALAGAIGYLIEVAVMKDINDKLNMGVLSGIIAGIVAGMLYNRYKDI
HHHHEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
KLPDYLAFFGGKRFVPIVTGVACLVLGIVFGYAWAPVQAAIDAAGHWLTTAGAVGAFVFG
CCCHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LLNRLLLVTGLHHILNSLAWFVFGTFTPPGGAAVTGDLHRFFAGDPTAGTFMTGFFPIMM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECHHHHHHCCCCCCCHHHHHHHHHHH
FGLPAACLAMLHEAPKERRALVGGLLFSMALTSFLTGVTEPIEFSFMFLAPVLYAIHAVL
HCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
TGLSLAICSALGIKLGFTFSAGAIDYVLNYGLSTKGWEAIPIGIAYSVVYYGLFRFFIRK
HHHHHHHHHHHHHHCCEEECCHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHH
FNMATPGREQETPDVANESFSADGFVAPVGGSAATSGTRAQRYIAALGGAANLSVVDACT
HCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHH
TRLRLSVVDSEKVSDNELKSIGARGVLKRGGGNVQVIIGPEADIIADEIRTTIASGGAAA
HHEEEEEECCCCCCHHHHHHHCCCHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCC
ASPAKPAVASAPVVTAATAATAAAGNGPLDPDPLRWLAVFGGATNVVSLEAVAATRLRIV
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCEEEHHHHHHHEEEEE
VRDASAIDRQRLDTLNVAWVSTDTFHIVVGDAAPRYAQQLSTRLTATA
EECHHHHHHHHCCEEEEEEEECCEEEEEECCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3284790; 3056518; 8905232; 9278503 [H]