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The map label for this gene is exoA [H]

Identifier: 186474828

GI number: 186474828

Start: 66149

End: 66928

Strand: Direct

Name: exoA [H]

Synonym: Bphy_0057

Alternate gene names: 186474828

Gene position: 66149-66928 (Clockwise)

Preceding gene: 186474827

Following gene: 186474836

Centisome position: 1.9

GC content: 59.49

Gene sequence:

>780_bases
ATGTTGCGCGTGATCACGGCCAACCTGAACGGCATCCGTTCGGCGGCCAAGAAGGGCTTTTTCGATTGGTTCGGCGAGCA
GAAGGCCGATGTCGTATGCGTGCAGGAAATCAAATGCTCGCAGGACGACATGACGCCAGAGTTCCTCGCGCCGCACGGTT
TCACCGGCTATTTCCAGCATGCAGTGAAAAAGGGCTACAGCGGTGCGGGTCTCTATACGCGTCACGAACCGGATGAAGTC
GTGATCGGCTTCGGCAGCGAAGAGTTCGATCCCGAGGGGCGCTACGTGGAAGCGCGCTTCGGCAATCTGTCGGTGATTTC
GGTGTATGTGCCGTCGGGTTCGAGCGGCGACGAGCGTCAGCAGGCGAAGTACCGTTTCATGGACGAGTTCATGCCGCATC
TCGCGGAATTATCGAGGGAGCGTGAAGTCATCGTGTGCGGCGACGTGAACATCGTGCACAAGGAAATCGACATCAAGAAC
TGGAAAGGCAATCAGAAGAATTCGGGCTGCCTGCCGGAAGAGCGCGCGTGGCTCACGAAGCTGTTCGACGAAGTCGGCTA
CGTCGACGTGTTCCGCACGCTCGACCAGCGGCCCGAGCAGTACACGTGGTGGAGCAATCGCGGCCAGGCGTACGCGAAGA
ACGTGGGTTGGCGTATCGATTACCAGATCGCGACGCCGGGCGTCGCGGCCACGGCGAAGCGCGTCGATGTGTTTCGCGAT
ATCAAGTTCAGCGATCACGCGCCCGTGACCGTCGACTATGCTCACAAGCTGGCGAAATAG

Upstream 100 bases:

>100_bases
GCTGAAGCTCGAGTTTCCTCCGGCAAAGGAATCGCTGAAGGGCGTGATCGTCGAATGATCCTCACCTCGTCGAATAGACG
AACCACAATGAAGGAAAAAC

Downstream 100 bases:

>100_bases
GCATTCCCAGACGCGCTGCGGGAATCAGTCGCTCACAGCCGTTCGCCGCGGGCGTCCGATGCCCGCGTAGTCGGGCAGCG
CATCCACGGTCTTGCCGACC

Product: exodeoxyribonuclease III Xth

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MLRVITANLNGIRSAAKKGFFDWFGEQKADVVCVQEIKCSQDDMTPEFLAPHGFTGYFQHAVKKGYSGAGLYTRHEPDEV
VIGFGSEEFDPEGRYVEARFGNLSVISVYVPSGSSGDERQQAKYRFMDEFMPHLAELSREREVIVCGDVNIVHKEIDIKN
WKGNQKNSGCLPEERAWLTKLFDEVGYVDVFRTLDQRPEQYTWWSNRGQAYAKNVGWRIDYQIATPGVAATAKRVDVFRD
IKFSDHAPVTVDYAHKLAK

Sequences:

>Translated_259_residues
MLRVITANLNGIRSAAKKGFFDWFGEQKADVVCVQEIKCSQDDMTPEFLAPHGFTGYFQHAVKKGYSGAGLYTRHEPDEV
VIGFGSEEFDPEGRYVEARFGNLSVISVYVPSGSSGDERQQAKYRFMDEFMPHLAELSREREVIVCGDVNIVHKEIDIKN
WKGNQKNSGCLPEERAWLTKLFDEVGYVDVFRTLDQRPEQYTWWSNRGQAYAKNVGWRIDYQIATPGVAATAKRVDVFRD
IKFSDHAPVTVDYAHKLAK
>Mature_259_residues
MLRVITANLNGIRSAAKKGFFDWFGEQKADVVCVQEIKCSQDDMTPEFLAPHGFTGYFQHAVKKGYSGAGLYTRHEPDEV
VIGFGSEEFDPEGRYVEARFGNLSVISVYVPSGSSGDERQQAKYRFMDEFMPHLAELSREREVIVCGDVNIVHKEIDIKN
WKGNQKNSGCLPEERAWLTKLFDEVGYVDVFRTLDQRPEQYTWWSNRGQAYAKNVGWRIDYQIATPGVAATAKRVDVFRD
IKFSDHAPVTVDYAHKLAK

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=253, Percent_Identity=35.5731225296443, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI18375503, Length=253, Percent_Identity=35.5731225296443, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI18375501, Length=253, Percent_Identity=35.5731225296443, Blast_Score=176, Evalue=2e-44,
Organism=Escherichia coli, GI1788046, Length=267, Percent_Identity=30.7116104868914, Blast_Score=104, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI71989536, Length=255, Percent_Identity=30.9803921568627, Blast_Score=138, Evalue=3e-33,
Organism=Drosophila melanogaster, GI221330655, Length=251, Percent_Identity=32.6693227091633, Blast_Score=146, Evalue=1e-35,
Organism=Drosophila melanogaster, GI17136678, Length=251, Percent_Identity=32.6693227091633, Blast_Score=145, Evalue=2e-35,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29503; Mature: 29503

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRVITANLNGIRSAAKKGFFDWFGEQKADVVCVQEIKCSQDDMTPEFLAPHGFTGYFQH
CEEEEEECCHHHHHHHHHCHHHHHCCCCCCEEEEEHHCCCCCCCCHHHHCCCCCHHHHHH
AVKKGYSGAGLYTRHEPDEVVIGFGSEEFDPEGRYVEARFGNLSVISVYVPSGSSGDERQ
HHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCEEEEEEEECCCCCCCHHH
QAKYRFMDEFMPHLAELSREREVIVCGDVNIVHKEIDIKNWKGNQKNSGCLPEERAWLTK
HHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHH
LFDEVGYVDVFRTLDQRPEQYTWWSNRGQAYAKNVGWRIDYQIATPGVAATAKRVDVFRD
HHHHCCHHHHHHHHHCCCCCCEEECCCCCEEHHCCCEEEEEEEECCCHHHHHHHHHHHHC
IKFSDHAPVTVDYAHKLAK
CCCCCCCCEEEHHHHHHCC
>Mature Secondary Structure
MLRVITANLNGIRSAAKKGFFDWFGEQKADVVCVQEIKCSQDDMTPEFLAPHGFTGYFQH
CEEEEEECCHHHHHHHHHCHHHHHCCCCCCEEEEEHHCCCCCCCCHHHHCCCCCHHHHHH
AVKKGYSGAGLYTRHEPDEVVIGFGSEEFDPEGRYVEARFGNLSVISVYVPSGSSGDERQ
HHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCEEEEEEEECCCCCCCHHH
QAKYRFMDEFMPHLAELSREREVIVCGDVNIVHKEIDIKNWKGNQKNSGCLPEERAWLTK
HHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHH
LFDEVGYVDVFRTLDQRPEQYTWWSNRGQAYAKNVGWRIDYQIATPGVAATAKRVDVFRD
HHHHCCHHHHHHHHHCCCCCCEEECCCCCEEHHCCCEEEEEEEECCCHHHHHHHHHHHHC
IKFSDHAPVTVDYAHKLAK
CCCCCCCCEEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]