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The map label for this gene is rsmB [H]

Identifier: 186474810

GI number: 186474810

Start: 45662

End: 47023

Strand: Direct

Name: rsmB [H]

Synonym: Bphy_0039

Alternate gene names: 186474810

Gene position: 45662-47023 (Clockwise)

Preceding gene: 186474809

Following gene: 186474811

Centisome position: 1.31

GC content: 65.93

Gene sequence:

>1362_bases
ATGCTGCATCTCGCACCGGAATCGCTCGGTTTCGCGCTCGACTGCGCCGCGCAGGCCGTCGGCGCGGTGCGTCAGGGCGC
TGCGTTGCCCGCAGCCTTGCATTCAATCTTCGTGTCGTTGCCGGAGGGTGTCGCGACGGCGTCGCGCGGGGCCGTGCAGG
ACATCGCATACCGTACGATGCGCCGGCTAGGCACGGCAGAATGGCTCGTCGCGAAGCTGGTGCGCAAGGCGCCGCCGCCG
CACGTCGCGCATGTGCTCGCGTGTGCGCTTGCGTTGCTGGTCGACGAAGCGAGCAACGCGGCGTACACGCCCTTCACGGT
GGTCGATCAGGCCGTGAACGCAATCGGCGCGCGGCGCGAGTCGTCCTTCGCGAAAGGCCTGACGAACGCGGTGCTGCGCA
ATTTCCTGCGCGAACGCGAGACCTTGCTCGGCGACGCGCAGAAGGATGCCGTCGCTCACTGGAATTATCCCGTCTGGTGG
ATCGACGCCGTTCGCAACGCATGGCCCGACGCATGGCAAGACATCCTCACGGCAGGCAACAATCAAGGTCCGCTAACGCT
GCGGGTGAACGCGCGGCGCGCGACCGTCGATGCTTATCTGCAAACGTTGCGTGAGCATCAGATTTCTGCGACGCAAGCGG
GCGACCAAGCCGTGCGACTCGAAACACCGATGCCCGTCGATCGCATTCCCGGTTTCAACGAAGGCGTCGCCTCGGTTCAG
GACGCGGGCGCGCAACTCGCCGCGCAATGGCTGGGTGTGCGCGACGGCATGCGGGTGCTGGACGCGTGTGCCGCGCCCGG
TGGCAAGACGGGTCATCTTCTTGAACTCGCGGATATCGATCTGATCGCGCTAGAAAGCGACGCGACGCGCGCGCGCCGGA
TCGGCGAAAACCTGCAGCGCCTTGGATTGAGCGCGCACGTGCGCGTCGGCGATGCGGGCAGTCCCGAGCAATGGCACGAT
GCCGCCGATGCGCCGTTCGACCGCATCCTCGCCGATGTGCCGTGTTCGGCTTCGGGCATCGTGCGCCGTCATCCGGACAT
TCGCTGGCTGCGCCGCGCCTCGGATATTCCCGTGCTCGTCGAGGAACAGCGCCGCATCCTCGAAGCGCTCTGGACGCTCG
TCAAACCGGGCGGCGAGTTGCTGTACGTTACGTGCTCGATCTTTCCGGAAGAAGGCGAGTTGCAGGCCCAGTGGTTTGGA
AACCGGCACGCGGATGCGGTACGATTGGACGCGCCGGGGCAACTGCTCCCTGCAGTCGCCCGCGCGCCCGCCGACGTATC
GGCTGGTTCCCGCGCCGGACAAAGCACTGGTTCGAGTTTAGACCACGACGGATTCTTCTACGCGCGCTTTCAGAAACGGT
GA

Upstream 100 bases:

>100_bases
TGCGCGACACTTGGTGCGCACCGTTCTCTGCCCGTTCATGACCCGAAAGCCTTCTCAGCGTCCTGCCGCATCACCGCGTT
CGCGGGACACTCATCTGTCG

Downstream 100 bases:

>100_bases
TCATCAAACGCTTTCTTCCGCTACGGCTCGCGGCGGCGCTCTGGATTGCGCTGGCCTTCTGGCTGACGGCAGCCGGGCCG
GCGCGAGCCGAATCGATCGC

Product: sun protein

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MLHLAPESLGFALDCAAQAVGAVRQGAALPAALHSIFVSLPEGVATASRGAVQDIAYRTMRRLGTAEWLVAKLVRKAPPP
HVAHVLACALALLVDEASNAAYTPFTVVDQAVNAIGARRESSFAKGLTNAVLRNFLRERETLLGDAQKDAVAHWNYPVWW
IDAVRNAWPDAWQDILTAGNNQGPLTLRVNARRATVDAYLQTLREHQISATQAGDQAVRLETPMPVDRIPGFNEGVASVQ
DAGAQLAAQWLGVRDGMRVLDACAAPGGKTGHLLELADIDLIALESDATRARRIGENLQRLGLSAHVRVGDAGSPEQWHD
AADAPFDRILADVPCSASGIVRRHPDIRWLRRASDIPVLVEEQRRILEALWTLVKPGGELLYVTCSIFPEEGELQAQWFG
NRHADAVRLDAPGQLLPAVARAPADVSAGSRAGQSTGSSLDHDGFFYARFQKR

Sequences:

>Translated_453_residues
MLHLAPESLGFALDCAAQAVGAVRQGAALPAALHSIFVSLPEGVATASRGAVQDIAYRTMRRLGTAEWLVAKLVRKAPPP
HVAHVLACALALLVDEASNAAYTPFTVVDQAVNAIGARRESSFAKGLTNAVLRNFLRERETLLGDAQKDAVAHWNYPVWW
IDAVRNAWPDAWQDILTAGNNQGPLTLRVNARRATVDAYLQTLREHQISATQAGDQAVRLETPMPVDRIPGFNEGVASVQ
DAGAQLAAQWLGVRDGMRVLDACAAPGGKTGHLLELADIDLIALESDATRARRIGENLQRLGLSAHVRVGDAGSPEQWHD
AADAPFDRILADVPCSASGIVRRHPDIRWLRRASDIPVLVEEQRRILEALWTLVKPGGELLYVTCSIFPEEGELQAQWFG
NRHADAVRLDAPGQLLPAVARAPADVSAGSRAGQSTGSSLDHDGFFYARFQKR
>Mature_453_residues
MLHLAPESLGFALDCAAQAVGAVRQGAALPAALHSIFVSLPEGVATASRGAVQDIAYRTMRRLGTAEWLVAKLVRKAPPP
HVAHVLACALALLVDEASNAAYTPFTVVDQAVNAIGARRESSFAKGLTNAVLRNFLRERETLLGDAQKDAVAHWNYPVWW
IDAVRNAWPDAWQDILTAGNNQGPLTLRVNARRATVDAYLQTLREHQISATQAGDQAVRLETPMPVDRIPGFNEGVASVQ
DAGAQLAAQWLGVRDGMRVLDACAAPGGKTGHLLELADIDLIALESDATRARRIGENLQRLGLSAHVRVGDAGSPEQWHD
AADAPFDRILADVPCSASGIVRRHPDIRWLRRASDIPVLVEEQRRILEALWTLVKPGGELLYVTCSIFPEEGELQAQWFG
NRHADAVRLDAPGQLLPAVARAPADVSAGSRAGQSTGSSLDHDGFFYARFQKR

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=281, Percent_Identity=27.0462633451957, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI76150623, Length=281, Percent_Identity=27.0462633451957, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI32698918, Length=189, Percent_Identity=30.6878306878307, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI11545785, Length=346, Percent_Identity=26.0115606936416, Blast_Score=76, Evalue=8e-14,
Organism=Escherichia coli, GI2367212, Length=446, Percent_Identity=38.1165919282511, Blast_Score=274, Evalue=9e-75,
Organism=Escherichia coli, GI87081985, Length=315, Percent_Identity=27.9365079365079, Blast_Score=96, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17536757, Length=288, Percent_Identity=29.1666666666667, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6324268, Length=349, Percent_Identity=24.3553008595989, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6319447, Length=308, Percent_Identity=26.9480519480519, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24668781, Length=239, Percent_Identity=32.6359832635983, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356513, Length=239, Percent_Identity=32.6359832635983, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI22024126, Length=290, Percent_Identity=25.5172413793103, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19920494, Length=173, Percent_Identity=32.3699421965318, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 48942; Mature: 48942

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHLAPESLGFALDCAAQAVGAVRQGAALPAALHSIFVSLPEGVATASRGAVQDIAYRTM
CCCCCCHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHH
RRLGTAEWLVAKLVRKAPPPHVAHVLACALALLVDEASNAAYTPFTVVDQAVNAIGARRE
HHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
SSFAKGLTNAVLRNFLRERETLLGDAQKDAVAHWNYPVWWIDAVRNAWPDAWQDILTAGN
HHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCEECCCCCHHHHHHHHCCHHHHHHHHHCCC
NQGPLTLRVNARRATVDAYLQTLREHQISATQAGDQAVRLETPMPVDRIPGFNEGVASVQ
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHCCCCCCHHHHHH
DAGAQLAAQWLGVRDGMRVLDACAAPGGKTGHLLELADIDLIALESDATRARRIGENLQR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCEEEEECCCHHHHHHHHHHHHH
LGLSAHVRVGDAGSPEQWHDAADAPFDRILADVPCSASGIVRRHPDIRWLRRASDIPVLV
CCCEEEEEECCCCCCHHHHCCCCCCHHHHHHCCCCCCCCCHHCCCCHHHHHHCCCCCCHH
EEQRRILEALWTLVKPGGELLYVTCSIFPEEGELQAQWFGNRHADAVRLDAPGQLLPAVA
HHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEHEECCCCCCCEEEECCCCHHHHHHH
RAPADVSAGSRAGQSTGSSLDHDGFFYARFQKR
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECC
>Mature Secondary Structure
MLHLAPESLGFALDCAAQAVGAVRQGAALPAALHSIFVSLPEGVATASRGAVQDIAYRTM
CCCCCCHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHH
RRLGTAEWLVAKLVRKAPPPHVAHVLACALALLVDEASNAAYTPFTVVDQAVNAIGARRE
HHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
SSFAKGLTNAVLRNFLRERETLLGDAQKDAVAHWNYPVWWIDAVRNAWPDAWQDILTAGN
HHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCEECCCCCHHHHHHHHCCHHHHHHHHHCCC
NQGPLTLRVNARRATVDAYLQTLREHQISATQAGDQAVRLETPMPVDRIPGFNEGVASVQ
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHCCCCCCHHHHHH
DAGAQLAAQWLGVRDGMRVLDACAAPGGKTGHLLELADIDLIALESDATRARRIGENLQR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCEEEEECCCHHHHHHHHHHHHH
LGLSAHVRVGDAGSPEQWHDAADAPFDRILADVPCSASGIVRRHPDIRWLRRASDIPVLV
CCCEEEEEECCCCCCHHHHCCCCCCHHHHHHCCCCCCCCCHHCCCCHHHHHHCCCCCCHH
EEQRRILEALWTLVKPGGELLYVTCSIFPEEGELQAQWFGNRHADAVRLDAPGQLLPAVA
HHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEHEECCCCCCCEEEECCCCHHHHHHH
RAPADVSAGSRAGQSTGSSLDHDGFFYARFQKR
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]