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The map label for this gene is folE

Identifier: 183222791

GI number: 183222791

Start: 3572326

End: 3572877

Strand: Reverse

Name: folE

Synonym: LEPBI_I3451

Alternate gene names: 183222791

Gene position: 3572877-3572326 (Counterclockwise)

Preceding gene: 183222798

Following gene: 183222790

Centisome position: 99.26

GC content: 39.49

Gene sequence:

>552_bases
ATGGAAAACTTAATCGAAGAAATCCTAAAACAAATTGGTGAAGATCCAAACAGAGAAGGTCTCGTCAAAACTCCTAATCG
AGTGAAAAAAGCTTATGATTTTTTAACGAGTGGGTACAAGGCAGACATCAACCAATTGGTCAATGGCGCCATTTTTGAAG
AAAATACCACAGGTATGGTTTTGGTTCGTGATATTGAAATGTATTCCTTATGCGAACACCACTTACTCCCTTTTTATGGA
AGGGCTCATGTCGCATACATTCCCAATAAAAAAATCATTGGGATCAGTAAAATTCCACGAATCGTAGATGTGTTTGCCAG
AAGACTGCAAGTACAAGAAAGACTTACCGACCAAATTGCACAGGCCATCCAAGAAACATTGGACCCTTTGGGTGTTGGTG
TTGTCATCAAAGCAAAACATTTGTGTATGATGATGCGAGGAGTCGAAAAACAAAACTCAGAATTATTCACTTCCAGTTTG
CTTGGTCTCTTCAAAACGGATCCTACTACTCGAAGCGAATTTTTAGATCTCATCCGAACCGGATCGCACTAA

Upstream 100 bases:

>100_bases
TTTCCTTAAGAGTCAAGTCTAGTCAAATAACTAGTGAATATTCTGCATTCAAATTTTGAAAGGCAGGTACACAAGGAACT
TACTTTCAAAAAGGAATCGG

Downstream 100 bases:

>100_bases
GTTCAATTTTTAAATTCTTTTTTTTCGATTCATGCTGGACTTTTTTTTAAAAAAGTTCAGTCTTTCTCCACTAGAGGGAC
ACAATGCCGAAAGAAAGAAT

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MENLIEEILKQIGEDPNREGLVKTPNRVKKAYDFLTSGYKADINQLVNGAIFEENTTGMVLVRDIEMYSLCEHHLLPFYG
RAHVAYIPNKKIIGISKIPRIVDVFARRLQVQERLTDQIAQAIQETLDPLGVGVVIKAKHLCMMMRGVEKQNSELFTSSL
LGLFKTDPTTRSEFLDLIRTGSH

Sequences:

>Translated_183_residues
MENLIEEILKQIGEDPNREGLVKTPNRVKKAYDFLTSGYKADINQLVNGAIFEENTTGMVLVRDIEMYSLCEHHLLPFYG
RAHVAYIPNKKIIGISKIPRIVDVFARRLQVQERLTDQIAQAIQETLDPLGVGVVIKAKHLCMMMRGVEKQNSELFTSSL
LGLFKTDPTTRSEFLDLIRTGSH
>Mature_183_residues
MENLIEEILKQIGEDPNREGLVKTPNRVKKAYDFLTSGYKADINQLVNGAIFEENTTGMVLVRDIEMYSLCEHHLLPFYG
RAHVAYIPNKKIIGISKIPRIVDVFARRLQVQERLTDQIAQAIQETLDPLGVGVVIKAKHLCMMMRGVEKQNSELFTSSL
LGLFKTDPTTRSEFLDLIRTGSH

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=173, Percent_Identity=56.6473988439306, Blast_Score=224, Evalue=2e-59,
Organism=Homo sapiens, GI4503949, Length=173, Percent_Identity=56.6473988439306, Blast_Score=224, Evalue=2e-59,
Organism=Homo sapiens, GI66932970, Length=131, Percent_Identity=55.7251908396947, Blast_Score=167, Evalue=4e-42,
Organism=Homo sapiens, GI66932972, Length=131, Percent_Identity=55.7251908396947, Blast_Score=167, Evalue=5e-42,
Organism=Escherichia coli, GI1788476, Length=178, Percent_Identity=43.8202247191011, Blast_Score=120, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17560486, Length=178, Percent_Identity=55.6179775280899, Blast_Score=222, Evalue=9e-59,
Organism=Saccharomyces cerevisiae, GI6321706, Length=175, Percent_Identity=54.2857142857143, Blast_Score=204, Evalue=8e-54,
Organism=Drosophila melanogaster, GI24656782, Length=173, Percent_Identity=52.6011560693642, Blast_Score=203, Evalue=5e-53,
Organism=Drosophila melanogaster, GI24656796, Length=173, Percent_Identity=52.6011560693642, Blast_Score=202, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24656791, Length=173, Percent_Identity=52.6011560693642, Blast_Score=202, Evalue=1e-52,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): GCH1_LEPBA (B0S8X8)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001964377.1
- ProteinModelPortal:   B0S8X8
- SMR:   B0S8X8
- GeneID:   6390042
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_3333
- HOGENOM:   HBG370191
- OMA:   ARIVEMF
- ProtClustDB:   PRK09347
- BioCyc:   LBIF355278:LBF_3333-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234
- TIGRFAMs:   TIGR00063

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =3.5.4.16

Molecular weight: Translated: 20756; Mature: 20756

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENLIEEILKQIGEDPNREGLVKTPNRVKKAYDFLTSGYKADINQLVNGAIFEENTTGMV
CHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCHHEECCCCCEE
LVRDIEMYSLCEHHLLPFYGRAHVAYIPNKKIIGISKIPRIVDVFARRLQVQERLTDQIA
EEEHHHHHHHHHHHCCCCCCCEEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHH
QAIQETLDPLGVGVVIKAKHLCMMMRGVEKQNSELFTSSLLGLFKTDPTTRSEFLDLIRT
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHC
GSH
CCC
>Mature Secondary Structure
MENLIEEILKQIGEDPNREGLVKTPNRVKKAYDFLTSGYKADINQLVNGAIFEENTTGMV
CHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCHHEECCCCCEE
LVRDIEMYSLCEHHLLPFYGRAHVAYIPNKKIIGISKIPRIVDVFARRLQVQERLTDQIA
EEEHHHHHHHHHHHCCCCCCCEEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHH
QAIQETLDPLGVGVVIKAKHLCMMMRGVEKQNSELFTSSLLGLFKTDPTTRSEFLDLIRT
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHC
GSH
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA