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The map label for this gene is fadA1 [H]

Identifier: 183222745

GI number: 183222745

Start: 3523058

End: 3524272

Strand: Reverse

Name: fadA1 [H]

Synonym: LEPBI_I3403

Alternate gene names: 183222745

Gene position: 3524272-3523058 (Counterclockwise)

Preceding gene: 183222746

Following gene: 183222744

Centisome position: 97.91

GC content: 44.12

Gene sequence:

>1215_bases
ATGGGGAATTCCTATATTATTGATGCTGTCCGAACTCCGAGAGGAAAGGGCAAAAAACGCGGGACACTTGCATCCGTCCA
CCCACAAGAATTAGCTGCTGCCACATTAAAAGCCATCCAATCCCGTACAGGAATCGATCCAAAAACGGTAGAAGAAGTTG
TAATGGGTTGTGTATCCCAAGTCGCTGACCAAGCTGCATGTATCGCACGTTATGCGGTTATGGCGGCACATTGGCCAAAA
GATGTTCCTGGTTATACAGTAAACCGTTTTTGCGGATCTGGATTACAAGCACTGAACAACGTAGCAAACCATGTTGCGTC
TGGAGCGATGGAACTTGGAGTTGGTGGTGGAGTGGAATCCATGAGCCGAGTGAAAATGGGTGATGATATGATCGGTCGTG
ATTTTAACGTAGGTAACGATAAAATTGCTGCTCATTACAACCTAGTTCCACAAGGAATCTCTGCTGACTTAATTGCAACG
AAGTATGATATTTCTCGTGAAGAAGCAGATCGTTTTGCAGAATCTTCACAACAAAAAGCTCATGCTGCCATTCAAAACGG
ATACTTCAAAAAATCTGTGATCCCAATCACTTTGGATGATGGAACTGTTGTGACAGAGGAAGAGAACCCACGATTGGAAT
CTGATTATGCATTCCTTTCAAGCCTTGGTCCTGTTTTCAAAACGATTGGTGAAAAAGAACTCGATGCCATTGCACTTCGT
TCTTACCCAGAAGTGAAAAAAATCAACCACATCCATACACTTGGAAACTCATCCGGTATCGTGGATGGTGCTGCGGCGAT
TTTAGTAACGAACGATGATGGTTTGAAAAAATATGGTTTAAAACCTCGTGCAAAAATCCTTGCAACGGTAGCGACTGGTG
AAGACCCAACCATCATGTTAACTGGACCTGTTTCTGCTTCACAAAAAGCTTTGAAACAAGCAGGCCTCAGTGTAAAAGAC
ATCGACCTTTGGGAAATCAACGAAGCTTTCGCATCTGTTGTGTTATACGTAAAGAAAACACTCGGAATCGATGAATCCAA
AATCAATGTGAACGGTGGAGCGATTGCTCTTGGTCACCCACTCGGAGCAACAGGTGCTATCCTCACTGGAACGGTGCTTG
ACGAGTTGGAACGAAGAGACCTTCGTTACGGCCTTATCACTCTTTGTATTGGTGGTGGTATGGGGATTGCAACAATCATC
GAACGATTGAAGTAA

Upstream 100 bases:

>100_bases
ACAAATTCACAAAAACTATGACGAAATGATGATTTTTTTACTTTTCTTGTGAGGCGAACTCTGTACGTTTTTCCTAGAAC
ACCCAGAGAGAGGTAAACCC

Downstream 100 bases:

>100_bases
AACCAATTTCTCTTTCAAATTCAAGTTTAGGCCCCGTGTATTGGGGTTTAAACTTGGTTCTCATTTTCCTCTTTCAAATC
ACTTCGAAAAACTAACTAAA

Product: acetyl-CoA acetyltransferase

Products: CoA; 3-oxoacyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MGNSYIIDAVRTPRGKGKKRGTLASVHPQELAAATLKAIQSRTGIDPKTVEEVVMGCVSQVADQAACIARYAVMAAHWPK
DVPGYTVNRFCGSGLQALNNVANHVASGAMELGVGGGVESMSRVKMGDDMIGRDFNVGNDKIAAHYNLVPQGISADLIAT
KYDISREEADRFAESSQQKAHAAIQNGYFKKSVIPITLDDGTVVTEEENPRLESDYAFLSSLGPVFKTIGEKELDAIALR
SYPEVKKINHIHTLGNSSGIVDGAAAILVTNDDGLKKYGLKPRAKILATVATGEDPTIMLTGPVSASQKALKQAGLSVKD
IDLWEINEAFASVVLYVKKTLGIDESKINVNGGAIALGHPLGATGAILTGTVLDELERRDLRYGLITLCIGGGMGIATII
ERLK

Sequences:

>Translated_404_residues
MGNSYIIDAVRTPRGKGKKRGTLASVHPQELAAATLKAIQSRTGIDPKTVEEVVMGCVSQVADQAACIARYAVMAAHWPK
DVPGYTVNRFCGSGLQALNNVANHVASGAMELGVGGGVESMSRVKMGDDMIGRDFNVGNDKIAAHYNLVPQGISADLIAT
KYDISREEADRFAESSQQKAHAAIQNGYFKKSVIPITLDDGTVVTEEENPRLESDYAFLSSLGPVFKTIGEKELDAIALR
SYPEVKKINHIHTLGNSSGIVDGAAAILVTNDDGLKKYGLKPRAKILATVATGEDPTIMLTGPVSASQKALKQAGLSVKD
IDLWEINEAFASVVLYVKKTLGIDESKINVNGGAIALGHPLGATGAILTGTVLDELERRDLRYGLITLCIGGGMGIATII
ERLK
>Mature_403_residues
GNSYIIDAVRTPRGKGKKRGTLASVHPQELAAATLKAIQSRTGIDPKTVEEVVMGCVSQVADQAACIARYAVMAAHWPKD
VPGYTVNRFCGSGLQALNNVANHVASGAMELGVGGGVESMSRVKMGDDMIGRDFNVGNDKIAAHYNLVPQGISADLIATK
YDISREEADRFAESSQQKAHAAIQNGYFKKSVIPITLDDGTVVTEEENPRLESDYAFLSSLGPVFKTIGEKELDAIALRS
YPEVKKINHIHTLGNSSGIVDGAAAILVTNDDGLKKYGLKPRAKILATVATGEDPTIMLTGPVSASQKALKQAGLSVKDI
DLWEINEAFASVVLYVKKTLGIDESKINVNGGAIALGHPLGATGAILTGTVLDELERRDLRYGLITLCIGGGMGIATIIE
RLK

Specific function: Subunit Of The Multifunctional Enzyme Complex Of The Fatty Acid Degradation Cycle. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=410, Percent_Identity=37.5609756097561, Blast_Score=252, Evalue=4e-67,
Organism=Homo sapiens, GI4501853, Length=397, Percent_Identity=37.5314861460957, Blast_Score=225, Evalue=6e-59,
Organism=Homo sapiens, GI148539872, Length=413, Percent_Identity=35.5932203389831, Blast_Score=219, Evalue=4e-57,
Organism=Homo sapiens, GI4557237, Length=411, Percent_Identity=33.5766423357664, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI4504327, Length=445, Percent_Identity=28.9887640449438, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI194353979, Length=388, Percent_Identity=28.8659793814433, Blast_Score=135, Evalue=9e-32,
Organism=Escherichia coli, GI48994986, Length=411, Percent_Identity=39.4160583941606, Blast_Score=269, Evalue=2e-73,
Organism=Escherichia coli, GI1787663, Length=425, Percent_Identity=37.4117647058824, Blast_Score=249, Evalue=3e-67,
Organism=Escherichia coli, GI1788554, Length=423, Percent_Identity=40.1891252955083, Blast_Score=245, Evalue=3e-66,
Organism=Escherichia coli, GI87082165, Length=414, Percent_Identity=36.4734299516908, Blast_Score=231, Evalue=8e-62,
Organism=Escherichia coli, GI1788683, Length=429, Percent_Identity=27.7389277389277, Blast_Score=141, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI133906874, Length=413, Percent_Identity=35.1089588377724, Blast_Score=226, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI25147385, Length=411, Percent_Identity=30.6569343065693, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17535921, Length=411, Percent_Identity=32.360097323601, Blast_Score=174, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI17551802, Length=446, Percent_Identity=28.9237668161435, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17535917, Length=407, Percent_Identity=28.7469287469287, Blast_Score=128, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6322031, Length=403, Percent_Identity=35.2357320099256, Blast_Score=207, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6325229, Length=419, Percent_Identity=32.6968973747017, Blast_Score=172, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24655093, Length=421, Percent_Identity=35.8669833729216, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI17648125, Length=415, Percent_Identity=35.421686746988, Blast_Score=226, Evalue=3e-59,
Organism=Drosophila melanogaster, GI24640423, Length=412, Percent_Identity=33.0097087378641, Blast_Score=174, Evalue=7e-44,
Organism=Drosophila melanogaster, GI17137578, Length=443, Percent_Identity=29.3453724604966, Blast_Score=166, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.16

Molecular weight: Translated: 42871; Mature: 42739

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNSYIIDAVRTPRGKGKKRGTLASVHPQELAAATLKAIQSRTGIDPKTVEEVVMGCVSQ
CCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
VADQAACIARYAVMAAHWPKDVPGYTVNRFCGSGLQALNNVANHVASGAMELGVGGGVES
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCHHH
MSRVKMGDDMIGRDFNVGNDKIAAHYNLVPQGISADLIATKYDISREEADRFAESSQQKA
HHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
HAAIQNGYFKKSVIPITLDDGTVVTEEENPRLESDYAFLSSLGPVFKTIGEKELDAIALR
HHHHHCCCEECCEEEEEECCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHC
SYPEVKKINHIHTLGNSSGIVDGAAAILVTNDDGLKKYGLKPRAKILATVATGEDPTIML
CCCCHHHHHHHHCCCCCCCCCCCEEEEEEECCCCHHHCCCCCCHHEEEEEECCCCCEEEE
TGPVSASQKALKQAGLSVKDIDLWEINEAFASVVLYVKKTLGIDESKINVNGGAIALGHP
ECCCCHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEECCC
LGATGAILTGTVLDELERRDLRYGLITLCIGGGMGIATIIERLK
CCCCCHHHHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHCC
>Mature Secondary Structure 
GNSYIIDAVRTPRGKGKKRGTLASVHPQELAAATLKAIQSRTGIDPKTVEEVVMGCVSQ
CCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
VADQAACIARYAVMAAHWPKDVPGYTVNRFCGSGLQALNNVANHVASGAMELGVGGGVES
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCHHH
MSRVKMGDDMIGRDFNVGNDKIAAHYNLVPQGISADLIATKYDISREEADRFAESSQQKA
HHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
HAAIQNGYFKKSVIPITLDDGTVVTEEENPRLESDYAFLSSLGPVFKTIGEKELDAIALR
HHHHHCCCEECCEEEEEECCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHC
SYPEVKKINHIHTLGNSSGIVDGAAAILVTNDDGLKKYGLKPRAKILATVATGEDPTIML
CCCCHHHHHHHHCCCCCCCCCCCEEEEEEECCCCHHHCCCCCCHHEEEEEECCCCCEEEE
TGPVSASQKALKQAGLSVKDIDLWEINEAFASVVLYVKKTLGIDESKINVNGGAIALGHP
ECCCCHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEECCC
LGATGAILTGTVLDELERRDLRYGLITLCIGGGMGIATIIERLK
CCCCCHHHHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acyl-CoA; acetyl-CoA

Specific reaction: acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA

General reaction: Acyl group transfer; Claisen condensation [C]

Inhibitor: Tris (hydroxy methyl) aminomethane [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]