Definition Leptospira biflexa serovar Patoc strain 'Patoc 1 (Paris)' chromosome chromosome I, complete sequence.
Accession NC_010602
Length 3,599,677

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The map label for this gene is metG

Identifier: 183222652

GI number: 183222652

Start: 3419438

End: 3421483

Strand: Reverse

Name: metG

Synonym: LEPBI_I3307

Alternate gene names: 183222652

Gene position: 3421483-3419438 (Counterclockwise)

Preceding gene: 183222655

Following gene: 183222651

Centisome position: 95.05

GC content: 39.15

Gene sequence:

>2046_bases
ATGTCGAAACACATTTTAGTTACAAGTGCTTTGCCTTATGCAAACGGCTCTATCCATTTAGGCCATATATTGGAAGCGGT
CCAAACTGACATTTGGGTACGCTTCCAAAAGTTGATTGGAAATGAATGTTATTTTTTCTGTGCGGATGATACACACGGAA
CCCCCATCATGATCGCTGCTAAAAAAGCAGGGAAAACTCCTGAGTCCATGATTGAGGAAGTTCAAAAAGAACATTACAAA
GATCTGACATCCTTTGGCGTTGAGTATGATAATTATTACACAACCAATTCGGAAGAGAACAAAAAGTTTTCCGAATCCAT
TTACCTAACTCTTAAAAAAAACGGACATATCGTTGCTCGTAACATAGAACAGTCGTATTGCGAACATGACAAAATGTTTT
TGCCTGACAGATTCATCAAAGGGACCTGTCCAAAATGTGGTGCCAAAGACCAATACGGAGATTCTTGCGAAGTCTGTGGA
ACAAGTTACTCACCTAAAGATTTAAAAGATTCATACTGTTCCATTTGCGGAACCACACCTGTGCTAAGAGAATCAAAACA
TTTGTTCTTTAAGTTACAAGACTTTCAAAACCAACTTAAAAACTGGATGGAAGAAGGGAATCGATTGAATGAAGGTGCCC
AGAAGAAATTACAAGAATGGTTTACATCAGGTTTACAAGAGTGGGATATCAGTCGTGACGGTCCTTACTTTGGTTTTGCG
ATCCCGGAAGAAGAAAACAAATACTTTTATGTATGGTTGGATGCACCCATCGGTTATATGGCTTCTTCCTTAAACCATCT
GAAGGATGAAAGAAAATTCAATGAGTTTTGGAAAGAAGGGAAAGGGGAAATTGTCCATTTCATCGGAAAAGACATTTTGT
ATTTCCATGGATTGTTTTGGCCTGCGATGCTTATGGGTTCAGGATACAAAGCACCGAGCCAATTGAATGTACATGGTTTT
TTAACTGTCAATGGCGAAAAGATGTCGAAATCACGTGGGACATTTATCAATGCATCCACGTTTGCTAAGTATTTAGATGT
GGAACATTTTCGCTTTTATTTGGCATGTCGTTTGGGTTCCGGAATGGAAGACGTCGACATTTCCTTTGATGATTTTGTTT
CCCGGGTAAATTCAGACCTGATAGGAAATTTGGTGAACTTGGTTTCTCGAGTTTCCACTTCCATTCTCGATAAAATGGAT
AGAAAATTGGGGATCTTGTCAACGGAAGGCAAATCATTGGTCTCAGAATTACTCTCCAAAGAAACAGAAATTCGGGAAGC
GTATGTGTCACGTAACTATTCCAAGGTGATGCGTGATATCACAGGTCTTGGGGACAAAGTGAATAAGTACGTAAATGATT
ATGCTCCTTGGAACCTGATCAAAACAGATGTGGAAAAAGCAAGAGAGGTTGTGACCACTTCCCTCAATTGCGCCAAAATA
TTATTTACTTATCTGGCGCCAGTGACTCCGAAAATTGTCCATTCGCTCGCGGACTTATTTCAAATTCCAAATTTGAGTTT
TTTGAATTTAACAGAGACCATCGAAAACAAAGTTCTTGGTCCCTACCAAATGTTATCCAAACGTGTTGAGGAAAAAAATA
TTACCATTATGATTACAGAAACGAAAGAAACGTTTGAAAAATCAAACCCTGAGAAAGCAAAACAAGATCCATCTAAGTCC
AATACAAACGAAGTAAAGTCGGCGACCGTTTCCGAAGATGGATTCATCACAATTGATGAATTGTCAAAAGTGGAACTTCG
AGTTGGTCTCATCAAAGAAGCAAATCCTGTGGAAGGAGCCGACAAACTTTTGTTTGTAAAAGTAGATTTAGGAGAAAAAG
GCATCAAAAACGTATTTGCGGGAATCAAAGCAAGCTACACAGCCGAAGAATTGGTGGGCAAAAAAGTAGTTGTTGTCGCC
AATTTAAAACCACGCCAAATGAAGTTTGGTTTATCAGAAGCGATGTTACTTGCGTCAGGAAAGGACAAAACATTATCCTT
GTTTGTTCCTGACCGCGATGCAAGTCCTGGTGACCTTTTGAAATAA

Upstream 100 bases:

>100_bases
GTTCTCTCACTTCCTTCAACTTCATTTTTTCTCTCTTTTCTTGATTTTTCTCAAAAATAGGCTGAATTGGAAAATCCCAT
TGGGAAACTGGAATTAATCT

Downstream 100 bases:

>100_bases
ACAATGGCAACAAAGTCAGAACAAATATTAAGGGAGAAAGAAATCCAAGGGATTAAGTTTAGTCTCTATGGAAAGATGAT
TGTTTTTTCCTTATTGACCA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 681; Mature: 680

Protein sequence:

>681_residues
MSKHILVTSALPYANGSIHLGHILEAVQTDIWVRFQKLIGNECYFFCADDTHGTPIMIAAKKAGKTPESMIEEVQKEHYK
DLTSFGVEYDNYYTTNSEENKKFSESIYLTLKKNGHIVARNIEQSYCEHDKMFLPDRFIKGTCPKCGAKDQYGDSCEVCG
TSYSPKDLKDSYCSICGTTPVLRESKHLFFKLQDFQNQLKNWMEEGNRLNEGAQKKLQEWFTSGLQEWDISRDGPYFGFA
IPEEENKYFYVWLDAPIGYMASSLNHLKDERKFNEFWKEGKGEIVHFIGKDILYFHGLFWPAMLMGSGYKAPSQLNVHGF
LTVNGEKMSKSRGTFINASTFAKYLDVEHFRFYLACRLGSGMEDVDISFDDFVSRVNSDLIGNLVNLVSRVSTSILDKMD
RKLGILSTEGKSLVSELLSKETEIREAYVSRNYSKVMRDITGLGDKVNKYVNDYAPWNLIKTDVEKAREVVTTSLNCAKI
LFTYLAPVTPKIVHSLADLFQIPNLSFLNLTETIENKVLGPYQMLSKRVEEKNITIMITETKETFEKSNPEKAKQDPSKS
NTNEVKSATVSEDGFITIDELSKVELRVGLIKEANPVEGADKLLFVKVDLGEKGIKNVFAGIKASYTAEELVGKKVVVVA
NLKPRQMKFGLSEAMLLASGKDKTLSLFVPDRDASPGDLLK

Sequences:

>Translated_681_residues
MSKHILVTSALPYANGSIHLGHILEAVQTDIWVRFQKLIGNECYFFCADDTHGTPIMIAAKKAGKTPESMIEEVQKEHYK
DLTSFGVEYDNYYTTNSEENKKFSESIYLTLKKNGHIVARNIEQSYCEHDKMFLPDRFIKGTCPKCGAKDQYGDSCEVCG
TSYSPKDLKDSYCSICGTTPVLRESKHLFFKLQDFQNQLKNWMEEGNRLNEGAQKKLQEWFTSGLQEWDISRDGPYFGFA
IPEEENKYFYVWLDAPIGYMASSLNHLKDERKFNEFWKEGKGEIVHFIGKDILYFHGLFWPAMLMGSGYKAPSQLNVHGF
LTVNGEKMSKSRGTFINASTFAKYLDVEHFRFYLACRLGSGMEDVDISFDDFVSRVNSDLIGNLVNLVSRVSTSILDKMD
RKLGILSTEGKSLVSELLSKETEIREAYVSRNYSKVMRDITGLGDKVNKYVNDYAPWNLIKTDVEKAREVVTTSLNCAKI
LFTYLAPVTPKIVHSLADLFQIPNLSFLNLTETIENKVLGPYQMLSKRVEEKNITIMITETKETFEKSNPEKAKQDPSKS
NTNEVKSATVSEDGFITIDELSKVELRVGLIKEANPVEGADKLLFVKVDLGEKGIKNVFAGIKASYTAEELVGKKVVVVA
NLKPRQMKFGLSEAMLLASGKDKTLSLFVPDRDASPGDLLK
>Mature_680_residues
SKHILVTSALPYANGSIHLGHILEAVQTDIWVRFQKLIGNECYFFCADDTHGTPIMIAAKKAGKTPESMIEEVQKEHYKD
LTSFGVEYDNYYTTNSEENKKFSESIYLTLKKNGHIVARNIEQSYCEHDKMFLPDRFIKGTCPKCGAKDQYGDSCEVCGT
SYSPKDLKDSYCSICGTTPVLRESKHLFFKLQDFQNQLKNWMEEGNRLNEGAQKKLQEWFTSGLQEWDISRDGPYFGFAI
PEEENKYFYVWLDAPIGYMASSLNHLKDERKFNEFWKEGKGEIVHFIGKDILYFHGLFWPAMLMGSGYKAPSQLNVHGFL
TVNGEKMSKSRGTFINASTFAKYLDVEHFRFYLACRLGSGMEDVDISFDDFVSRVNSDLIGNLVNLVSRVSTSILDKMDR
KLGILSTEGKSLVSELLSKETEIREAYVSRNYSKVMRDITGLGDKVNKYVNDYAPWNLIKTDVEKAREVVTTSLNCAKIL
FTYLAPVTPKIVHSLADLFQIPNLSFLNLTETIENKVLGPYQMLSKRVEEKNITIMITETKETFEKSNPEKAKQDPSKSN
TNEVKSATVSEDGFITIDELSKVELRVGLIKEANPVEGADKLLFVKVDLGEKGIKNVFAGIKASYTAEELVGKKVVVVAN
LKPRQMKFGLSEAMLLASGKDKTLSLFVPDRDASPGDLLK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=526, Percent_Identity=29.467680608365, Blast_Score=236, Evalue=4e-62,
Organism=Homo sapiens, GI24308436, Length=556, Percent_Identity=25.5395683453237, Blast_Score=176, Evalue=8e-44,
Organism=Escherichia coli, GI1788432, Length=692, Percent_Identity=46.242774566474, Blast_Score=636, Evalue=0.0,
Organism=Escherichia coli, GI1789455, Length=95, Percent_Identity=40, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17540794, Length=615, Percent_Identity=27.9674796747967, Blast_Score=254, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI212645096, Length=535, Percent_Identity=23.5514018691589, Blast_Score=152, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI71992781, Length=411, Percent_Identity=22.6277372262774, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321704, Length=557, Percent_Identity=28.3662477558348, Blast_Score=217, Evalue=6e-57,
Organism=Saccharomyces cerevisiae, GI6321610, Length=543, Percent_Identity=23.2044198895028, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24655311, Length=515, Percent_Identity=30.0970873786408, Blast_Score=243, Evalue=2e-64,
Organism=Drosophila melanogaster, GI21358483, Length=521, Percent_Identity=25.7197696737044, Blast_Score=171, Evalue=1e-42,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_LEPBA (B0SHA8)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001964240.1
- ProteinModelPortal:   B0SHA8
- GeneID:   6387761
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_3193
- HOGENOM:   HBG721667
- OMA:   SAYQPEQ
- ProtClustDB:   PRK00133
- BioCyc:   LBIF355278:LBF_3193-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 76974; Mature: 76843

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 330-330

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MSKHILVTSALPYANGSIHLGHILEAVQTDIWVRFQKLIGNECYFFCADDTHGTPIMIAA
CCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEE
KKAGKTPESMIEEVQKEHYKDLTSFGVEYDNYYTTNSEENKKFSESIYLTLKKNGHIVAR
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHCCEEEEEEECCCEEEEE
NIEQSYCEHDKMFLPDRFIKGTCPKCGAKDQYGDSCEVCGTSYSPKDLKDSYCSICGTTP
CCHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHCCCCH
VLRESKHLFFKLQDFQNQLKNWMEEGNRLNEGAQKKLQEWFTSGLQEWDISRDGPYFGFA
HHHCCCHHEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHCCCCCCCCEEEEE
IPEEENKYFYVWLDAPIGYMASSLNHLKDERKFNEFWKEGKGEIVHFIGKDILYFHGLFW
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHH
PAMLMGSGYKAPSQLNVHGFLTVNGEKMSKSRGTFINASTFAKYLDVEHFRFYLACRLGS
HHHHHCCCCCCCCCCCEEEEEEECCHHHHCCCCCEEEHHHHHHHHHHHHHHHEEEEECCC
GMEDVDISFDDFVSRVNSDLIGNLVNLVSRVSTSILDKMDRKLGILSTEGKSLVSELLSK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHHHHH
ETEIREAYVSRNYSKVMRDITGLGDKVNKYVNDYAPWNLIKTDVEKAREVVTTSLNCAKI
HHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHH
LFTYLAPVTPKIVHSLADLFQIPNLSFLNLTETIENKVLGPYQMLSKRVEEKNITIMITE
HHHHHCCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCHHHHHHHHHHHCCEEEEEEC
TKETFEKSNPEKAKQDPSKSNTNEVKSATVSEDGFITIDELSKVELRVGLIKEANPVEGA
CHHHHHCCCCHHHHCCCCCCCCHHHHHCEECCCCCEECCCCCHHHEEEEEECCCCCCCCC
DKLLFVKVDLGEKGIKNVFAGIKASYTAEELVGKKVVVVANLKPRQMKFGLSEAMLLASG
CCEEEEEECCCCHHHHHHHHHCCCCCCHHHHCCCEEEEEECCCCHHHHCCHHHHHHEECC
KDKTLSLFVPDRDASPGDLLK
CCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
SKHILVTSALPYANGSIHLGHILEAVQTDIWVRFQKLIGNECYFFCADDTHGTPIMIAA
CCCEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEE
KKAGKTPESMIEEVQKEHYKDLTSFGVEYDNYYTTNSEENKKFSESIYLTLKKNGHIVAR
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHCCEEEEEEECCCEEEEE
NIEQSYCEHDKMFLPDRFIKGTCPKCGAKDQYGDSCEVCGTSYSPKDLKDSYCSICGTTP
CCHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHCCCCH
VLRESKHLFFKLQDFQNQLKNWMEEGNRLNEGAQKKLQEWFTSGLQEWDISRDGPYFGFA
HHHCCCHHEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHCCCCCCCCEEEEE
IPEEENKYFYVWLDAPIGYMASSLNHLKDERKFNEFWKEGKGEIVHFIGKDILYFHGLFW
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHH
PAMLMGSGYKAPSQLNVHGFLTVNGEKMSKSRGTFINASTFAKYLDVEHFRFYLACRLGS
HHHHHCCCCCCCCCCCEEEEEEECCHHHHCCCCCEEEHHHHHHHHHHHHHHHEEEEECCC
GMEDVDISFDDFVSRVNSDLIGNLVNLVSRVSTSILDKMDRKLGILSTEGKSLVSELLSK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHHHHH
ETEIREAYVSRNYSKVMRDITGLGDKVNKYVNDYAPWNLIKTDVEKAREVVTTSLNCAKI
HHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHH
LFTYLAPVTPKIVHSLADLFQIPNLSFLNLTETIENKVLGPYQMLSKRVEEKNITIMITE
HHHHHCCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCHHHHHHHHHHHCCEEEEEEC
TKETFEKSNPEKAKQDPSKSNTNEVKSATVSEDGFITIDELSKVELRVGLIKEANPVEGA
CHHHHHCCCCHHHHCCCCCCCCHHHHHCEECCCCCEECCCCCHHHEEEEEECCCCCCCCC
DKLLFVKVDLGEKGIKNVFAGIKASYTAEELVGKKVVVVANLKPRQMKFGLSEAMLLASG
CCEEEEEECCCCHHHHHHHHHCCCCCCHHHHCCCEEEEEECCCCHHHHCCHHHHHHEECC
KDKTLSLFVPDRDASPGDLLK
CCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA