Click here to switch to the map view.

The map label for this gene is prfA

Identifier: 183222627

GI number: 183222627

Start: 3394384

End: 3395445

Strand: Reverse

Name: prfA

Synonym: LEPBI_I3282

Alternate gene names: 183222627

Gene position: 3395445-3394384 (Counterclockwise)

Preceding gene: 183222630

Following gene: 183222626

Centisome position: 94.33

GC content: 42.28

Gene sequence:

>1062_bases
ATGATAGATAGATTGAAAAAAATTCAAGAAAAATACCTGCGTATCGAGGATGAGCTCGCAAAGGCCACCGCATCGGATAC
ATTAAAGAATCTATCGAAAGAGAGATCTCGCCTAACTCCCGTATATACAAAAGCAGATGAATATTTAAAAATTACTAAAG
ATTGCCAAGATGCAAAATCCTTATTAGAATCAGAAAATGATCCAGACATGCATTCCATGTTAAAATCAGAAATCGAAGAA
GGAGAAAAAAAATTAGAAGAACTGGCAAAAGAACTCGAAATTATGTTATTACCACCTGATCCCAATTCAGGGAAAAGTAT
TCTAGTCGAGATTCGTGCTGGAACCGGTGGGGAAGAATCGGGATTATTTTGTGCCGATTTATTTCGAATGTACAACAAGT
ATGCCGATAAAAAGGGACTGCGCGTTGAGATCATTGATATGAGCCAAACAGGTATTGGCGGATATAAAGAAATCGTATTC
TCGCTTGATGATGATAAAGCCTATGATTTGTTTAAATTTGAATCTGGGACACACCGTGTACAAAGGATACCTGAAACTGA
ATCAGGCGGAAGGATTCACACTTCTGCTGTAACAGTAGCCATCCTACCAGAAGCCGAAGAAAAAGAAGTTGAGATCAAAG
AAAGTGACCTTCGAATTGACGTTTATCGCTCGTCAGGTGCTGGAGGTCAGCACGTCAACACAACTGACTCGGCGGTTCGT
ATCACACACATTCCAACTGGGATTGTAGTCGCCTCCCAAGAAGAACGTTCCCAAATCAAGAACCGCGATAAAGCAATGAG
AGTACTCAGAGCAAGGATCGCTGACCAAGCAGCTGAGACTGCTAAACTCAGCGCTGATGCTTTGAAAAAAGCGCAGGTTG
GTTCGGGAGACAGATCAGAACGCATTCGAACGTATAACTTCCCTCAAGGCCGATGCACGGACCACAGGATCGGATTCACG
AGTCATAATCTACCAGCCATCATGGAAGGGGATTTGGATGAATTAATTGATGCCTTGATCCAAGAAGATCGGTCCAAACG
ATTAGCAGAAGCCAAGGCTTAA

Upstream 100 bases:

>100_bases
ATTGGATTTGCCCTGAGAGGGAAGTTCGTTCACGGGTTTCTTAAATCTCCGTTAGATTTTAGTTTATCAAAAACAGGCAT
TCTGTATCCTTTCCATAGAC

Downstream 100 bases:

>100_bases
AAAAAAACAAAATTTACCAAAGGAATTCGATTGAATCAACTGATTTGATTGTTATTCCTTTTAGTAGGATGAAGACCTCA
TGAAATTTCTGCAATTAGGG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MIDRLKKIQEKYLRIEDELAKATASDTLKNLSKERSRLTPVYTKADEYLKITKDCQDAKSLLESENDPDMHSMLKSEIEE
GEKKLEELAKELEIMLLPPDPNSGKSILVEIRAGTGGEESGLFCADLFRMYNKYADKKGLRVEIIDMSQTGIGGYKEIVF
SLDDDKAYDLFKFESGTHRVQRIPETESGGRIHTSAVTVAILPEAEEKEVEIKESDLRIDVYRSSGAGGQHVNTTDSAVR
ITHIPTGIVVASQEERSQIKNRDKAMRVLRARIADQAAETAKLSADALKKAQVGSGDRSERIRTYNFPQGRCTDHRIGFT
SHNLPAIMEGDLDELIDALIQEDRSKRLAEAKA

Sequences:

>Translated_353_residues
MIDRLKKIQEKYLRIEDELAKATASDTLKNLSKERSRLTPVYTKADEYLKITKDCQDAKSLLESENDPDMHSMLKSEIEE
GEKKLEELAKELEIMLLPPDPNSGKSILVEIRAGTGGEESGLFCADLFRMYNKYADKKGLRVEIIDMSQTGIGGYKEIVF
SLDDDKAYDLFKFESGTHRVQRIPETESGGRIHTSAVTVAILPEAEEKEVEIKESDLRIDVYRSSGAGGQHVNTTDSAVR
ITHIPTGIVVASQEERSQIKNRDKAMRVLRARIADQAAETAKLSADALKKAQVGSGDRSERIRTYNFPQGRCTDHRIGFT
SHNLPAIMEGDLDELIDALIQEDRSKRLAEAKA
>Mature_353_residues
MIDRLKKIQEKYLRIEDELAKATASDTLKNLSKERSRLTPVYTKADEYLKITKDCQDAKSLLESENDPDMHSMLKSEIEE
GEKKLEELAKELEIMLLPPDPNSGKSILVEIRAGTGGEESGLFCADLFRMYNKYADKKGLRVEIIDMSQTGIGGYKEIVF
SLDDDKAYDLFKFESGTHRVQRIPETESGGRIHTSAVTVAILPEAEEKEVEIKESDLRIDVYRSSGAGGQHVNTTDSAVR
ITHIPTGIVVASQEERSQIKNRDKAMRVLRARIADQAAETAKLSADALKKAQVGSGDRSERIRTYNFPQGRCTDHRIGFT
SHNLPAIMEGDLDELIDALIQEDRSKRLAEAKA

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=289, Percent_Identity=44.636678200692, Blast_Score=258, Evalue=6e-69,
Organism=Homo sapiens, GI34577120, Length=332, Percent_Identity=37.9518072289157, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI166795305, Length=194, Percent_Identity=42.2680412371134, Blast_Score=171, Evalue=1e-42,
Organism=Escherichia coli, GI1787462, Length=355, Percent_Identity=48.7323943661972, Blast_Score=315, Evalue=2e-87,
Organism=Escherichia coli, GI2367172, Length=314, Percent_Identity=36.624203821656, Blast_Score=196, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17542784, Length=260, Percent_Identity=35, Blast_Score=160, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6321295, Length=317, Percent_Identity=41.6403785488959, Blast_Score=243, Evalue=3e-65,
Organism=Drosophila melanogaster, GI19921226, Length=296, Percent_Identity=43.5810810810811, Blast_Score=241, Evalue=4e-64,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_LEPBA (B0SH84)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001964216.1
- ProteinModelPortal:   B0SH84
- SMR:   B0SH84
- GeneID:   6388113
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_3169
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- ProtClustDB:   PRK00591
- BioCyc:   LBIF355278:LBF_3169-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 39502; Mature: 39502

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDRLKKIQEKYLRIEDELAKATASDTLKNLSKERSRLTPVYTKADEYLKITKDCQDAKS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHH
LLESENDPDMHSMLKSEIEEGEKKLEELAKELEIMLLPPDPNSGKSILVEIRAGTGGEES
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCEEEEEEECCCCCCCC
GLFCADLFRMYNKYADKKGLRVEIIDMSQTGIGGYKEIVFSLDDDKAYDLFKFESGTHRV
CCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHEEEECCCCCCEEEEEECCCCHHH
QRIPETESGGRIHTSAVTVAILPEAEEKEVEIKESDLRIDVYRSSGAGGQHVNTTDSAVR
HCCCCCCCCCEEEEEEEEEEEECCCCCCCCEEECCCEEEEEEECCCCCCCCCCCCCCEEE
ITHIPTGIVVASQEERSQIKNRDKAMRVLRARIADQAAETAKLSADALKKAQVGSGDRSE
EEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
RIRTYNFPQGRCTDHRIGFTSHNLPAIMEGDLDELIDALIQEDRSKRLAEAKA
CCEECCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIDRLKKIQEKYLRIEDELAKATASDTLKNLSKERSRLTPVYTKADEYLKITKDCQDAKS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHH
LLESENDPDMHSMLKSEIEEGEKKLEELAKELEIMLLPPDPNSGKSILVEIRAGTGGEES
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCEEEEEEECCCCCCCC
GLFCADLFRMYNKYADKKGLRVEIIDMSQTGIGGYKEIVFSLDDDKAYDLFKFESGTHRV
CCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHEEEECCCCCCEEEEEECCCCHHH
QRIPETESGGRIHTSAVTVAILPEAEEKEVEIKESDLRIDVYRSSGAGGQHVNTTDSAVR
HCCCCCCCCCEEEEEEEEEEEECCCCCCCCEEECCCEEEEEEECCCCCCCCCCCCCCEEE
ITHIPTGIVVASQEERSQIKNRDKAMRVLRARIADQAAETAKLSADALKKAQVGSGDRSE
EEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
RIRTYNFPQGRCTDHRIGFTSHNLPAIMEGDLDELIDALIQEDRSKRLAEAKA
CCEECCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA