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The map label for this gene is hisS

Identifier: 183222581

GI number: 183222581

Start: 3348738

End: 3350051

Strand: Reverse

Name: hisS

Synonym: LEPBI_I3236

Alternate gene names: 183222581

Gene position: 3350051-3348738 (Counterclockwise)

Preceding gene: 183222582

Following gene: 183222580

Centisome position: 93.07

GC content: 37.21

Gene sequence:

>1314_bases
TTGAAAGAACAAAAACTAACAACAGAAAATTATAAGGGCACTCGGGATTTTTATCCAGAAGATATGCGCCTTCGTAACTA
TTTATTTTCGGTCATGAAAGATGTCGTGAGATCGTATGGGTACGAAGAGTATGATGGACCCATGGTGGAATCGTTAGATT
TATACCGTGCCAAAACGGGTGAAGAGATTGTTGGAAAACAAATTTATAACTTCATTGATAAAGGGGATCGAGAAGTTGCC
ATTCGACCCGAAATGACACCTACTGTTGCAAGGATGGTCGCAAAAAAGTTACGTGAATTGCCAAGACCGATTCGTTGGTT
TTCCATTCCCAATCTTTGGCGTTATGAACAACCTGGCCATGGAAGGCTCAGGGAACACTGGCAATTGAATGTCGATATGT
TTGGCGTTACTAGTAGTCGCGCAGAGCTTGAGATTTTATCTTTAGCTTGTGATATCCTCTTTGCTTTTGGGGCTCCAAAA
AACAGTTTCAAAGTCACCATCTCACACCGGTCACTCCTCGATGAATTCTTGTTAGACGGACTTAAGGTAAGTCCTAATCA
AGCACATGAAGTGTCCAAAATTTTGGACAAAAAAAATAAAATCACACAGGACGAATACGTCGCTTTAGTGACCAAAACAA
TTCCAAATGACTCTTCTGCTGTTTCCAAAATTGATTTATTTTTGAATGCTACGGTAAATTCGTTAAACCAAATACCAGGA
ATCAAAGAAGAAACCTTGGCTGCCATCAAGGGATTATTTGAAGACATCAAAACTATTGGACTTTCTGATATCATTCACTT
TGATCCATCTGTTGTCCGAGGATTTGATTATTATACAGGATTTATTTTTGAAATCTTTGATACCTCTCCGCAAAACAAAC
GTTCATTGTACGGTGGTGGAAGATATGATAATTTAATTGGACTTTTTTCTAATGAAGAGTTAACAGGAATTGGTTTTGGA
TTGGGAGACGTTACCTTACAAAATTTTTTAACGGCCCACAATCTGTTACCGAATTTTGCCAGCGATACAACGGTTTTTAT
TCCTCTATTGGATGATTCGTCTTTCGCAGAAAATCACAATTTTGCAAAAGAATTAAGAAAAGAAAAAATTTCTGCCGAGG
TTTCATTGGTTTCACAAAAAATGGGAAAACAACTTTCCTATGCTGAAAAAAAAGGATACAGATGGATTTTACTTCGAGGG
GAAGATGAAATCAAAACAAATACAGTAACCTTAAAAGATATGGCATCAAGAAACCAATTTACATTTAGTTTCTCGGAAGC
GCTTCAAAAGATTAAAGAAGAGCTTTCGAAATGA

Upstream 100 bases:

>100_bases
AAGGATCACTGGTGTAAGCGGAGATAAAAAAGCCAAAGAGATTTACCATAGTCTCATTGAATTTTTTAATTAAACTGCTC
GGTTCAATAGGAGCCAATCA

Downstream 100 bases:

>100_bases
ATTGGATTAACACATTAGATCTTAAACTTTCTACTTGGATCCAAAACCATTTACACCATAAAAATCTAAGTTGGGTTTTG
TCTCGAATCAATCGGGGTGA

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MKEQKLTTENYKGTRDFYPEDMRLRNYLFSVMKDVVRSYGYEEYDGPMVESLDLYRAKTGEEIVGKQIYNFIDKGDREVA
IRPEMTPTVARMVAKKLRELPRPIRWFSIPNLWRYEQPGHGRLREHWQLNVDMFGVTSSRAELEILSLACDILFAFGAPK
NSFKVTISHRSLLDEFLLDGLKVSPNQAHEVSKILDKKNKITQDEYVALVTKTIPNDSSAVSKIDLFLNATVNSLNQIPG
IKEETLAAIKGLFEDIKTIGLSDIIHFDPSVVRGFDYYTGFIFEIFDTSPQNKRSLYGGGRYDNLIGLFSNEELTGIGFG
LGDVTLQNFLTAHNLLPNFASDTTVFIPLLDDSSFAENHNFAKELRKEKISAEVSLVSQKMGKQLSYAEKKGYRWILLRG
EDEIKTNTVTLKDMASRNQFTFSFSEALQKIKEELSK

Sequences:

>Translated_437_residues
MKEQKLTTENYKGTRDFYPEDMRLRNYLFSVMKDVVRSYGYEEYDGPMVESLDLYRAKTGEEIVGKQIYNFIDKGDREVA
IRPEMTPTVARMVAKKLRELPRPIRWFSIPNLWRYEQPGHGRLREHWQLNVDMFGVTSSRAELEILSLACDILFAFGAPK
NSFKVTISHRSLLDEFLLDGLKVSPNQAHEVSKILDKKNKITQDEYVALVTKTIPNDSSAVSKIDLFLNATVNSLNQIPG
IKEETLAAIKGLFEDIKTIGLSDIIHFDPSVVRGFDYYTGFIFEIFDTSPQNKRSLYGGGRYDNLIGLFSNEELTGIGFG
LGDVTLQNFLTAHNLLPNFASDTTVFIPLLDDSSFAENHNFAKELRKEKISAEVSLVSQKMGKQLSYAEKKGYRWILLRG
EDEIKTNTVTLKDMASRNQFTFSFSEALQKIKEELSK
>Mature_437_residues
MKEQKLTTENYKGTRDFYPEDMRLRNYLFSVMKDVVRSYGYEEYDGPMVESLDLYRAKTGEEIVGKQIYNFIDKGDREVA
IRPEMTPTVARMVAKKLRELPRPIRWFSIPNLWRYEQPGHGRLREHWQLNVDMFGVTSSRAELEILSLACDILFAFGAPK
NSFKVTISHRSLLDEFLLDGLKVSPNQAHEVSKILDKKNKITQDEYVALVTKTIPNDSSAVSKIDLFLNATVNSLNQIPG
IKEETLAAIKGLFEDIKTIGLSDIIHFDPSVVRGFDYYTGFIFEIFDTSPQNKRSLYGGGRYDNLIGLFSNEELTGIGFG
LGDVTLQNFLTAHNLLPNFASDTTVFIPLLDDSSFAENHNFAKELRKEKISAEVSLVSQKMGKQLSYAEKKGYRWILLRG
EDEIKTNTVTLKDMASRNQFTFSFSEALQKIKEELSK

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI6996014, Length=461, Percent_Identity=27.114967462039, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI15029520, Length=459, Percent_Identity=25.9259259259259, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI1788861, Length=415, Percent_Identity=25.7831325301205, Blast_Score=124, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI71993693, Length=479, Percent_Identity=25.2609603340292, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71993686, Length=479, Percent_Identity=25.2609603340292, Blast_Score=127, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6325290, Length=447, Percent_Identity=26.6219239373602, Blast_Score=117, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24643061, Length=471, Percent_Identity=23.9915074309979, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24643059, Length=490, Percent_Identity=23.0612244897959, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_LEPBA (B0SH39)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001964171.1
- GeneID:   6387583
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_3124
- HOGENOM:   HBG616575
- OMA:   SIPQCWR
- ProtClustDB:   PRK00037
- BioCyc:   LBIF355278:LBF_3124-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 49906; Mature: 49906

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEQKLTTENYKGTRDFYPEDMRLRNYLFSVMKDVVRSYGYEEYDGPMVESLDLYRAKTG
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCC
EEIVGKQIYNFIDKGDREVAIRPEMTPTVARMVAKKLRELPRPIRWFSIPNLWRYEQPGH
HHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCC
GRLREHWQLNVDMFGVTSSRAELEILSLACDILFAFGAPKNSFKVTISHRSLLDEFLLDG
CCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHCC
LKVSPNQAHEVSKILDKKNKITQDEYVALVTKTIPNDSSAVSKIDLFLNATVNSLNQIPG
CCCCCCHHHHHHHHHHHHCCCCHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC
IKEETLAAIKGLFEDIKTIGLSDIIHFDPSVVRGFDYYTGFIFEIFDTSPQNKRSLYGGG
CCHHHHHHHHHHHHHHHHHCHHHHHHCCHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCC
RYDNLIGLFSNEELTGIGFGLGDVTLQNFLTAHNLLPNFASDTTVFIPLLDDSSFAENHN
CCCCEEEEECCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHCCH
FAKELRKEKISAEVSLVSQKMGKQLSYAEKKGYRWILLRGEDEIKTNTVTLKDMASRNQF
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCEEEEEECCCCCCCCEEEHHHHHCCCCE
TFSFSEALQKIKEELSK
EEEHHHHHHHHHHHHCC
>Mature Secondary Structure
MKEQKLTTENYKGTRDFYPEDMRLRNYLFSVMKDVVRSYGYEEYDGPMVESLDLYRAKTG
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCC
EEIVGKQIYNFIDKGDREVAIRPEMTPTVARMVAKKLRELPRPIRWFSIPNLWRYEQPGH
HHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCC
GRLREHWQLNVDMFGVTSSRAELEILSLACDILFAFGAPKNSFKVTISHRSLLDEFLLDG
CCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHCC
LKVSPNQAHEVSKILDKKNKITQDEYVALVTKTIPNDSSAVSKIDLFLNATVNSLNQIPG
CCCCCCHHHHHHHHHHHHCCCCHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC
IKEETLAAIKGLFEDIKTIGLSDIIHFDPSVVRGFDYYTGFIFEIFDTSPQNKRSLYGGG
CCHHHHHHHHHHHHHHHHHCHHHHHHCCHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCC
RYDNLIGLFSNEELTGIGFGLGDVTLQNFLTAHNLLPNFASDTTVFIPLLDDSSFAENHN
CCCCEEEEECCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHCCH
FAKELRKEKISAEVSLVSQKMGKQLSYAEKKGYRWILLRGEDEIKTNTVTLKDMASRNQF
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCEEEEEECCCCCCCCEEEHHHHHCCCCE
TFSFSEALQKIKEELSK
EEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA