Click here to switch to the map view.

The map label for this gene is algI [H]

Identifier: 183222442

GI number: 183222442

Start: 3208943

End: 3210313

Strand: Direct

Name: algI [H]

Synonym: LEPBI_I3096

Alternate gene names: 183222442

Gene position: 3208943-3210313 (Clockwise)

Preceding gene: 183222439

Following gene: 183222443

Centisome position: 89.15

GC content: 32.46

Gene sequence:

>1371_bases
ATGCTCTTCAATTCAATTGATTATTTCTACTTTTTTGTTACCTGTTATATCCTTTATTGGATTTCTCCTGCACGGTTTCG
GAAATATATCTTAATCATTTTTTCATTAATCTTTTATGGTTACTGGAGCGGTTCTTTTTTAATTCATTTCGTACTTTTCA
TTGTCATTACCCATCTTTGCGTTATCGGTATTTTAAAAACTAAAAAGAGAATATTTTTAATCCTTGGTGTATCCATTAAT
TTAATCAATTTATGTTTCTTTAAATATTTTTTAACTTATTTAAAGTATTTAATGTCAGAAGGGAATTTAACCATTCCGTT
CTTTCAAAGCCTTTCCGAATTTGTATTACCATTAGCAATTAGTTTTTATACGTTCCAAATGATTGCGTATCTCGTTGATG
CTTGGCGAGGAAAGTTCTCAGAATCTCCATTTATACACTTTTTACTTTTTATTTTATTTTTCCCTCAGCTGATCGCTGGC
CCTATCATGCGTCATGATGATTTTTATGATCAAATTGATCGATCAAAGTTAACTTTTGATTTTGTGCAACGTGGGATTTT
GCACATCATCTCTGGTTTGATCAAAAAAGTTCTCATTGCCGATCAAATTGCAAAAATGATTAATCCAGTGTATGCAAATC
CTGGAGAATATGACGGTGTCTCAATTCTATTGTCAACTTTCGCATTTTCTTTCCAAGTTTACGGTGATTTTTCGGGTTAC
ACTGATTTGGCGAGAGGATCAGCTTTTCTTTTAGGGTACGAGATTCCGGAAAACTTTCGATCCCCATTTTTATCAGTCAG
TTTCACAGAATTATGGAGCCGTTGGCATTATACACTTTCCACTTGGATTAGAGATTACTTATATATCCCATTGGGAGGAA
ATCGAGTCTCAGAATTAAGATACAATCTGAACACGATCTTAGTGATGTCACTTTCGGGATTGTGGCATGGAAATACTTAC
ACGTTTTTTCTTTGGGGATTTTTCCATGGAATCTTTCTTTCCATAGAAAGATTTGCTTTCGGTAGACCTGACCGTAAATC
CATGTCAAACTTGAAAAAGTTATTTGCAGTGATTTGGATTTTTAGTATTTTTTCAATTCTTGCTACTTTTTTTAGAATTG
ATACTCTCGAAAAAATTTCAACATTTTGGACTGCGATCGTAAACGTTAAAGGCAAAATCATTTATAAACCAGATTTTTGG
GGACTCATCATTGGAAGTTACATCATACAAATGATTGAATACAAACAATACTTTGCAGAAAAATTAAAACCATATACAAC
TTGGATCATTTTTTTCTTAAGTTTCTTCGTTTATATAGCTCTCAGCAAAATTGAAGCACCTGTAGAGACATTTATTTACT
TTCAGTTTTAA

Upstream 100 bases:

>100_bases
CGGCCATCTTGAGAACTGATCTTTCTGAATTTGAATATCTAGGCAAGTATGTTTGCATAAATGACTGGTAGAAAAAAATA
TTAGAGAAATTCTTGGGAAA

Downstream 100 bases:

>100_bases
AGGCATATATGAAATCGATATCAATTTATTCTTTCCCATTCTTAATTTTATTCAGCATCATCTTAATCGATAAGGTGATT
TGTATTCCAGATGTTCGAGA

Product: putative membrane bound 0-acyl transferase

Products: NA

Alternate protein names: Alginate biosynthesis protein AlgI [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MLFNSIDYFYFFVTCYILYWISPARFRKYILIIFSLIFYGYWSGSFLIHFVLFIVITHLCVIGILKTKKRIFLILGVSIN
LINLCFFKYFLTYLKYLMSEGNLTIPFFQSLSEFVLPLAISFYTFQMIAYLVDAWRGKFSESPFIHFLLFILFFPQLIAG
PIMRHDDFYDQIDRSKLTFDFVQRGILHIISGLIKKVLIADQIAKMINPVYANPGEYDGVSILLSTFAFSFQVYGDFSGY
TDLARGSAFLLGYEIPENFRSPFLSVSFTELWSRWHYTLSTWIRDYLYIPLGGNRVSELRYNLNTILVMSLSGLWHGNTY
TFFLWGFFHGIFLSIERFAFGRPDRKSMSNLKKLFAVIWIFSIFSILATFFRIDTLEKISTFWTAIVNVKGKIIYKPDFW
GLIIGSYIIQMIEYKQYFAEKLKPYTTWIIFFLSFFVYIALSKIEAPVETFIYFQF

Sequences:

>Translated_456_residues
MLFNSIDYFYFFVTCYILYWISPARFRKYILIIFSLIFYGYWSGSFLIHFVLFIVITHLCVIGILKTKKRIFLILGVSIN
LINLCFFKYFLTYLKYLMSEGNLTIPFFQSLSEFVLPLAISFYTFQMIAYLVDAWRGKFSESPFIHFLLFILFFPQLIAG
PIMRHDDFYDQIDRSKLTFDFVQRGILHIISGLIKKVLIADQIAKMINPVYANPGEYDGVSILLSTFAFSFQVYGDFSGY
TDLARGSAFLLGYEIPENFRSPFLSVSFTELWSRWHYTLSTWIRDYLYIPLGGNRVSELRYNLNTILVMSLSGLWHGNTY
TFFLWGFFHGIFLSIERFAFGRPDRKSMSNLKKLFAVIWIFSIFSILATFFRIDTLEKISTFWTAIVNVKGKIIYKPDFW
GLIIGSYIIQMIEYKQYFAEKLKPYTTWIIFFLSFFVYIALSKIEAPVETFIYFQF
>Mature_456_residues
MLFNSIDYFYFFVTCYILYWISPARFRKYILIIFSLIFYGYWSGSFLIHFVLFIVITHLCVIGILKTKKRIFLILGVSIN
LINLCFFKYFLTYLKYLMSEGNLTIPFFQSLSEFVLPLAISFYTFQMIAYLVDAWRGKFSESPFIHFLLFILFFPQLIAG
PIMRHDDFYDQIDRSKLTFDFVQRGILHIISGLIKKVLIADQIAKMINPVYANPGEYDGVSILLSTFAFSFQVYGDFSGY
TDLARGSAFLLGYEIPENFRSPFLSVSFTELWSRWHYTLSTWIRDYLYIPLGGNRVSELRYNLNTILVMSLSGLWHGNTY
TFFLWGFFHGIFLSIERFAFGRPDRKSMSNLKKLFAVIWIFSIFSILATFFRIDTLEKISTFWTAIVNVKGKIIYKPDFW
GLIIGSYIIQMIEYKQYFAEKLKPYTTWIIFFLSFFVYIALSKIEAPVETFIYFQF

Specific function: Together with AlgJ and AlgF, forms an inner membrane complex which probably interacts with the alginate polymerization- transport complex and adds acetyl groups at the O-2 and O-3 positions of polymannuronic acid. Acetylation of alginate is important for

COG id: COG1696

COG function: function code M; Predicted membrane protein involved in D-alanine export

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane-bound acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004299 [H]

Pfam domain/function: PF03062 MBOAT [H]

EC number: NA

Molecular weight: Translated: 53866; Mature: 53866

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFNSIDYFYFFVTCYILYWISPARFRKYILIIFSLIFYGYWSGSFLIHFVLFIVITHLC
CCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
VIGILKTKKRIFLILGVSINLINLCFFKYFLTYLKYLMSEGNLTIPFFQSLSEFVLPLAI
HHHHHHCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHH
SFYTFQMIAYLVDAWRGKFSESPFIHFLLFILFFPQLIAGPIMRHDDFYDQIDRSKLTFD
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCHHHCCCCHHHHHHHCHHHHH
FVQRGILHIISGLIKKVLIADQIAKMINPVYANPGEYDGVSILLSTFAFSFQVYGDFSGY
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHEEEECCCCC
TDLARGSAFLLGYEIPENFRSPFLSVSFTELWSRWHYTLSTWIRDYLYIPLGGNRVSELR
HHHHCCCEEEEEEECCHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHH
YNLNTILVMSLSGLWHGNTYTFFLWGFFHGIFLSIERFAFGRPDRKSMSNLKKLFAVIWI
HHHHHEEEEEECCCEECCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
FSIFSILATFFRIDTLEKISTFWTAIVNVKGKIIYKPDFWGLIIGSYIIQMIEYKQYFAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHH
KLKPYTTWIIFFLSFFVYIALSKIEAPVETFIYFQF
HHCHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEC
>Mature Secondary Structure
MLFNSIDYFYFFVTCYILYWISPARFRKYILIIFSLIFYGYWSGSFLIHFVLFIVITHLC
CCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
VIGILKTKKRIFLILGVSINLINLCFFKYFLTYLKYLMSEGNLTIPFFQSLSEFVLPLAI
HHHHHHCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHH
SFYTFQMIAYLVDAWRGKFSESPFIHFLLFILFFPQLIAGPIMRHDDFYDQIDRSKLTFD
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCHHHCCCCHHHHHHHCHHHHH
FVQRGILHIISGLIKKVLIADQIAKMINPVYANPGEYDGVSILLSTFAFSFQVYGDFSGY
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHEEEECCCCC
TDLARGSAFLLGYEIPENFRSPFLSVSFTELWSRWHYTLSTWIRDYLYIPLGGNRVSELR
HHHHCCCEEEEEEECCHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHH
YNLNTILVMSLSGLWHGNTYTFFLWGFFHGIFLSIERFAFGRPDRKSMSNLKKLFAVIWI
HHHHHEEEEEECCCEECCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
FSIFSILATFFRIDTLEKISTFWTAIVNVKGKIIYKPDFWGLIIGSYIIQMIEYKQYFAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHH
KLKPYTTWIIFFLSFFVYIALSKIEAPVETFIYFQF
HHCHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8636017; 10984043; 12003941; 11208804; 11179370 [H]