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The map label for this gene is yerD [H]

Identifier: 183222326

GI number: 183222326

Start: 3080833

End: 3082413

Strand: Direct

Name: yerD [H]

Synonym: LEPBI_I2978

Alternate gene names: 183222326

Gene position: 3080833-3082413 (Clockwise)

Preceding gene: 183222325

Following gene: 183222327

Centisome position: 85.59

GC content: 42.31

Gene sequence:

>1581_bases
TTGAGTTCGAAGATAGAAATGGATGCACAAATGATGAATCAAATTTTGACTTGGATCGAAACAAATCCTATCTCCTCAAC
ATGGATTGGGATCTTACTTTTTTTTGTGTTTGTTTTCCTTCGTGACATCACACAAAAAAAACATACCATTCAAAGAAACT
TTCCCATCGTTGGAAGGCTACGTTATTTTTTAGAAATGATTGGCCCAGAACTCAGGCAGTACTGGGTTGCCAATGACAAA
GAAGAAAGGCCATTTGACCGGACAGAAAGAAGTTGGATCTACGCAACAGCCAAAGGACAAAATAATAATTTTGGTTTTGG
AACAACAGAAATCCAATACGAACCTGGATACCCCATCATCAAACACAAAGCCTTTCCTTATCCGGAATCAAAAGCATATA
TCCATAACAATGACCCAAGTTGTATCCCATGCCTCAAAGTAATTGGTCCAAATCGGAAATTTCCTTACCGTCCCTATTCC
ATTGTGAATATCTCTGCTATGTCTTTTGGATCACTTGGAAAAAATGCCGTACTCGCATTGAATCGAGGCGCAAGAGATTC
CGGTTCTTACCATAACACTGGTGAGGGGGGGCTTAGTCATTATCATATGGAAGGTGCTGACATCGTTTGGCAAATTGGGA
CTGGCTATTTTGGTGCCAGAGACCATTCTGGAAAGTTTAACTTAGATCTGTTAAAAGAAAAAGTAGGAACAAATGCTTGT
GTGAAAATGATCGAAATCAAATTATCACAAGGCGCCAAACCTGGTAAAGGTGGTATTTTACCTGCCAAAAAAGTAAACGC
AGAAATTGCATCCATTCGCCATGTAGAAGAAGGCAAGGATTGTATCTCACCTAACTCCCATAGTGAATTTACCAATGTGA
AAGAACTGGTTCAATTCATTGAAACCATTGCCAACGGAACAGGTTTGCCTGTTGGTATCAAAAGTGCGGTGGGAGAAATT
GAATTTTGGCAAGAACTAGCAGATGAGATGAAACGCACCTCAAAAGGACCTGATTTTATCACCATCGATGGTGGGGAAGG
TGGAACGGGGGCGGCACCTCTTACTTACGCAGACCATGTTTCCTTACCGTTTAAAATTGGATTCCAACGAGTGTACACTC
TGTTCCAAAAAGAAGGATTATCGGAACAGATCGTTTGGATTGGATCAGGAAAATTGGGATTTCCCGACCGAGCCGTTGTT
GCAATTGCCATGGGTTGTGACCTCATCAATATTGCCAGAGAAGCGATGTTATCCATCGGTTGTATCCAAGCACAAAAATG
CCATACCGACCATTGCCCTGCAGGTGTTGCCACACAAAACTGGTGGTTACAGCGCGGAGTGGACCCAACCATCAAAGGGA
AACGTGCCGCAAAGTACATCCAAGGATTCAGAAAAGAATTACTCAGCTTGGCACATTCTTGTGGGTATGAACACCCAGGC
CAGTTCACAGGGCAAGACATCGAAATTAGTATGGGGATGAATCGTTACCAAACCCTTGAGGGACTACTCGGTTACAAACG
TGATGAGGTAAAATTTACCAAACTCCAAGACTATACCGTGTTTCCAAAACGACAAGCTTAG

Upstream 100 bases:

>100_bases
CCGCAAAAATACCTTATGTCTCAGGTTATATTTTTAAACCATTCACCAAAGAAAAACTTTTGGATTCTTTGGCCCATCTA
CAATCTTAGCTTGTCAATAC

Downstream 100 bases:

>100_bases
ATGAAAAAACTCATTTTATTCTCTTTACTATTCCTTGTTAGCTGCTTGTCTGAAGAAAGGAATACCATTAAAGAAGCAAA
AAATGGGTTTATCAATTTAA

Product: hypothetical protein

Products: L-glutamine; 2-oxoglutarate; reduced ferredoxin

Alternate protein names: NA

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSSKIEMDAQMMNQILTWIETNPISSTWIGILLFFVFVFLRDITQKKHTIQRNFPIVGRLRYFLEMIGPELRQYWVANDK
EERPFDRTERSWIYATAKGQNNNFGFGTTEIQYEPGYPIIKHKAFPYPESKAYIHNNDPSCIPCLKVIGPNRKFPYRPYS
IVNISAMSFGSLGKNAVLALNRGARDSGSYHNTGEGGLSHYHMEGADIVWQIGTGYFGARDHSGKFNLDLLKEKVGTNAC
VKMIEIKLSQGAKPGKGGILPAKKVNAEIASIRHVEEGKDCISPNSHSEFTNVKELVQFIETIANGTGLPVGIKSAVGEI
EFWQELADEMKRTSKGPDFITIDGGEGGTGAAPLTYADHVSLPFKIGFQRVYTLFQKEGLSEQIVWIGSGKLGFPDRAVV
AIAMGCDLINIAREAMLSIGCIQAQKCHTDHCPAGVATQNWWLQRGVDPTIKGKRAAKYIQGFRKELLSLAHSCGYEHPG
QFTGQDIEISMGMNRYQTLEGLLGYKRDEVKFTKLQDYTVFPKRQA

Sequences:

>Translated_526_residues
MSSKIEMDAQMMNQILTWIETNPISSTWIGILLFFVFVFLRDITQKKHTIQRNFPIVGRLRYFLEMIGPELRQYWVANDK
EERPFDRTERSWIYATAKGQNNNFGFGTTEIQYEPGYPIIKHKAFPYPESKAYIHNNDPSCIPCLKVIGPNRKFPYRPYS
IVNISAMSFGSLGKNAVLALNRGARDSGSYHNTGEGGLSHYHMEGADIVWQIGTGYFGARDHSGKFNLDLLKEKVGTNAC
VKMIEIKLSQGAKPGKGGILPAKKVNAEIASIRHVEEGKDCISPNSHSEFTNVKELVQFIETIANGTGLPVGIKSAVGEI
EFWQELADEMKRTSKGPDFITIDGGEGGTGAAPLTYADHVSLPFKIGFQRVYTLFQKEGLSEQIVWIGSGKLGFPDRAVV
AIAMGCDLINIAREAMLSIGCIQAQKCHTDHCPAGVATQNWWLQRGVDPTIKGKRAAKYIQGFRKELLSLAHSCGYEHPG
QFTGQDIEISMGMNRYQTLEGLLGYKRDEVKFTKLQDYTVFPKRQA
>Mature_525_residues
SSKIEMDAQMMNQILTWIETNPISSTWIGILLFFVFVFLRDITQKKHTIQRNFPIVGRLRYFLEMIGPELRQYWVANDKE
ERPFDRTERSWIYATAKGQNNNFGFGTTEIQYEPGYPIIKHKAFPYPESKAYIHNNDPSCIPCLKVIGPNRKFPYRPYSI
VNISAMSFGSLGKNAVLALNRGARDSGSYHNTGEGGLSHYHMEGADIVWQIGTGYFGARDHSGKFNLDLLKEKVGTNACV
KMIEIKLSQGAKPGKGGILPAKKVNAEIASIRHVEEGKDCISPNSHSEFTNVKELVQFIETIANGTGLPVGIKSAVGEIE
FWQELADEMKRTSKGPDFITIDGGEGGTGAAPLTYADHVSLPFKIGFQRVYTLFQKEGLSEQIVWIGSGKLGFPDRAVVA
IAMGCDLINIAREAMLSIGCIQAQKCHTDHCPAGVATQNWWLQRGVDPTIKGKRAAKYIQGFRKELLSLAHSCGYEHPGQ
FTGQDIEISMGMNRYQTLEGLLGYKRDEVKFTKLQDYTVFPKRQA

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0069

COG function: function code E; Glutamate synthase domain 2

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI308199519, Length=206, Percent_Identity=30.5825242718447, Blast_Score=96, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17570289, Length=297, Percent_Identity=29.2929292929293, Blast_Score=114, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6320030, Length=289, Percent_Identity=29.0657439446367, Blast_Score=113, Evalue=6e-26,
Organism=Drosophila melanogaster, GI28574881, Length=288, Percent_Identity=29.1666666666667, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24665539, Length=288, Percent_Identity=29.1666666666667, Blast_Score=108, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR002932 [H]

Pfam domain/function: PF01645 Glu_synthase [H]

EC number: 1.4.7.1

Molecular weight: Translated: 58769; Mature: 58638

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKIEMDAQMMNQILTWIETNPISSTWIGILLFFVFVFLRDITQKKHTIQRNFPIVGRL
CCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
RYFLEMIGPELRQYWVANDKEERPFDRTERSWIYATAKGQNNNFGFGTTEIQYEPGYPII
HHHHHHHCHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCCEE
KHKAFPYPESKAYIHNNDPSCIPCLKVIGPNRKFPYRPYSIVNISAMSFGSLGKNAVLAL
ECCCCCCCCCCEEEECCCCCHHHHHHHHCCCCCCCCCCEEEEEEEHHCCCCCCCCEEEEE
NRGARDSGSYHNTGEGGLSHYHMEGADIVWQIGTGYFGARDHSGKFNLDLLKEKVGTNAC
ECCCCCCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHCCHHH
VKMIEIKLSQGAKPGKGGILPAKKVNAEIASIRHVEEGKDCISPNSHSEFTNVKELVQFI
HHHHHHHHHCCCCCCCCCCCCHHHHCHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHH
ETIANGTGLPVGIKSAVGEIEFWQELADEMKRTSKGPDFITIDGGEGGTGAAPLTYADHV
HHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEECCCC
SLPFKIGFQRVYTLFQKEGLSEQIVWIGSGKLGFPDRAVVAIAMGCDLINIAREAMLSIG
CCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHEEEHHHCCHHHHHHHHHHHHHH
CIQAQKCHTDHCPAGVATQNWWLQRGVDPTIKGKRAAKYIQGFRKELLSLAHSCGYEHPG
HHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
QFTGQDIEISMGMNRYQTLEGLLGYKRDEVKFTKLQDYTVFPKRQA
CCCCCCEEEECCCCHHHHHHHHHCCCCCCEEEEEEECEECCCCCCC
>Mature Secondary Structure 
SSKIEMDAQMMNQILTWIETNPISSTWIGILLFFVFVFLRDITQKKHTIQRNFPIVGRL
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
RYFLEMIGPELRQYWVANDKEERPFDRTERSWIYATAKGQNNNFGFGTTEIQYEPGYPII
HHHHHHHCHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCCEE
KHKAFPYPESKAYIHNNDPSCIPCLKVIGPNRKFPYRPYSIVNISAMSFGSLGKNAVLAL
ECCCCCCCCCCEEEECCCCCHHHHHHHHCCCCCCCCCCEEEEEEEHHCCCCCCCCEEEEE
NRGARDSGSYHNTGEGGLSHYHMEGADIVWQIGTGYFGARDHSGKFNLDLLKEKVGTNAC
ECCCCCCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHCCHHH
VKMIEIKLSQGAKPGKGGILPAKKVNAEIASIRHVEEGKDCISPNSHSEFTNVKELVQFI
HHHHHHHHHCCCCCCCCCCCCHHHHCHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHH
ETIANGTGLPVGIKSAVGEIEFWQELADEMKRTSKGPDFITIDGGEGGTGAAPLTYADHV
HHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEECCCC
SLPFKIGFQRVYTLFQKEGLSEQIVWIGSGKLGFPDRAVVAIAMGCDLINIAREAMLSIG
CCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHEEEHHHCCHHHHHHHHHHHHHH
CIQAQKCHTDHCPAGVATQNWWLQRGVDPTIKGKRAAKYIQGFRKELLSLAHSCGYEHPG
HHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
QFTGQDIEISMGMNRYQTLEGLLGYKRDEVKFTKLQDYTVFPKRQA
CCCCCCEEEECCCCHHHHHHHHHCCCCCCEEEEEEECEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; oxidized ferredoxin

Specific reaction: 2 L-glutamate + 2 oxidized ferredoxin = L-glutamine + 2-oxoglutarate + 2 reduced ferredoxin

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9701819; 9384377; 8969499 [H]