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The map label for this gene is oprF [H]

Identifier: 183222022

GI number: 183222022

Start: 2760057

End: 2761913

Strand: Reverse

Name: oprF [H]

Synonym: LEPBI_I2664

Alternate gene names: 183222022

Gene position: 2761913-2760057 (Counterclockwise)

Preceding gene: 183222024

Following gene: 183222021

Centisome position: 76.73

GC content: 41.68

Gene sequence:

>1857_bases
ATGGCACGGATTCTTCTCATCATTCTCTTCCTTTTTGGGAGCGGGCTCACGAGTTCGCAAAGCATTAAAAATGGAATCCA
GGTTTTGGAGGGGGACCTTATCAATACCGGCCATCTTCTGCGGACCGAGAAACAAGTATTCCGCATTTCGAATACGATTT
TGCAAGAAGAACTCGCTTATTTGAGTGGGAAAAAGGTACGGATGTTATGCGATGTGCAAGGGGAAGTCTGTTCTCCCATC
CGTTATGAAATGGAACCCTTCGAAACAGGAAAAACTCCTGATTGGACCTTAAAAAAAATACCACGGTATGTGACTCAAGG
TCTCTTTTCCTTTAATCCCCAATGTACCCCGGATGGAAAGGTTTTGTTTTGGACAGCCCTTGTCCGCGAAGGGGGAAGAT
CCACACAAAAAGTTTGGGCTTCTAAAAGGGACCAATACGGATTTTGGATGGCAGGGGAACAATTGCCTTCACCATTAAAC
AATCGTTATCCCTCGGCAGTGATTTCTGCCTTACCTGGTGGGAATGAACTTTTTGTTTTTGGTAATTTTGGAGAAGAGGA
ACTACTCGACCAATTGAAACGAGAGATGACGCAGAAGTCTCAAATTGCCACAAGAGAAGCGGCCAATGCCAAAGAGTTCC
ATATTGTTCTCACAAAATTAGAAAACGAGTATAAAGAACGATCGGAAAAAATCCAAAACCGAGCACCACTTTACAAAACA
CGTAAAACTGAAACTGGTTGGACGTTACCAAGTCCCATTCCATTCCCTACTTTTTATAATTGGTACAAAAAGGCAGACAA
TCCCAACCAACAAGTATTTGGTGGTTCTGCCTTGTCTTCGAGTGGACGAACACTCATTTATTCAGCGCAACAGAAGAAAA
ATTTTGGTAAATTGGATTTATACGCCAGCATTCAAAATGAATCTGGAATCTTTGAAGAAGGGATCAATTTAGGTAGCACT
CTAAACACCGGAGAAGAGGAGATGGCTCCTTTCCTTGGACCCGATGATCGAACTTTGTATTTTTCATCTTCCGGAAGGAA
AGAAGGGATATCAATATTCATTTCGAGAAGGAATGGAGATTCCTGGACTATGTGGTCTGAACCACAAGAGTTGTCACCTA
ACCTAAGGGGTGTTAACTTTTTTAGTATTCCTGCTGTTGGCAATTGGGCTTATGTATCGAGAGATGGGGAACTTTTTATG
GCGGCCATCCCCCATCATTTCCAACCAGATCCCGTGGTTGTGATCAAAGGCAAAGTTGTAGATGAAGAGGGGAAGGCACT
TTCAGCCTTTGTGCAATACGAATCTCTCTTAAAGAAAAAAACAATAGGATCCACCATCAGTGATCCAAACACAGGTGAGT
TTAGCATCATATTACCCTACGAAGAAAATTATGGATTTTATGGGGAAAAAGAAGGTTACCTTCCAGTTTCACAAAACTTA
AATTTGGTGGGTAAAGAAAAGGAAGAGAAAGAAAAAACAGTTCTCCTCGTTTTACCGAAATTGAAAAAAGGAAACCAAAT
TGTGATGAACAATTTGTTTTTTGCCTTCCGTTCGTCTGAGTTTGCCAAAGAATCCGAACCAGAACTAGACAGATTGGCAG
GAATTTTGAAAAAAACGACCAATTTAAAGGTACTCATCGAAGGGCATACAGACAATGTTGGTACAAAGTCCGCCAACCAA
AAGTTATCCTTGGAACGTGCAAATTCAGTTGCCTTTTATTTGAAGTCTAAACATAAAATAGAGGATTCGAGGATTTCTGT
TGTGGGGCTTGGTCCATCGAACCCCATTGCTGAGAATGGATCGGAAGAAGGCCGTGCCGTCAACCGTCGGGTAGTTTTTA
AAATTTTGGAAGAGTAA

Upstream 100 bases:

>100_bases
AAAAGGGGTTTTTTCCCGAAGGAGACTGTAATGCCGCTAGCTTGGATCATAGGTCAATTTTCTAAGGGAATCGGAGGTTT
CAACCGACATTTCGTATGGT

Downstream 100 bases:

>100_bases
TACAGTGGATATCAAAAATATCAATATACCCAAAGTCAATGTAGACACCCAAAAAATGATGGGGGCAGTGGATGGCCTCG
TGGACAAAATCCCCCCACAA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 618; Mature: 617

Protein sequence:

>618_residues
MARILLIILFLFGSGLTSSQSIKNGIQVLEGDLINTGHLLRTEKQVFRISNTILQEELAYLSGKKVRMLCDVQGEVCSPI
RYEMEPFETGKTPDWTLKKIPRYVTQGLFSFNPQCTPDGKVLFWTALVREGGRSTQKVWASKRDQYGFWMAGEQLPSPLN
NRYPSAVISALPGGNELFVFGNFGEEELLDQLKREMTQKSQIATREAANAKEFHIVLTKLENEYKERSEKIQNRAPLYKT
RKTETGWTLPSPIPFPTFYNWYKKADNPNQQVFGGSALSSSGRTLIYSAQQKKNFGKLDLYASIQNESGIFEEGINLGST
LNTGEEEMAPFLGPDDRTLYFSSSGRKEGISIFISRRNGDSWTMWSEPQELSPNLRGVNFFSIPAVGNWAYVSRDGELFM
AAIPHHFQPDPVVVIKGKVVDEEGKALSAFVQYESLLKKKTIGSTISDPNTGEFSIILPYEENYGFYGEKEGYLPVSQNL
NLVGKEKEEKEKTVLLVLPKLKKGNQIVMNNLFFAFRSSEFAKESEPELDRLAGILKKTTNLKVLIEGHTDNVGTKSANQ
KLSLERANSVAFYLKSKHKIEDSRISVVGLGPSNPIAENGSEEGRAVNRRVVFKILEE

Sequences:

>Translated_618_residues
MARILLIILFLFGSGLTSSQSIKNGIQVLEGDLINTGHLLRTEKQVFRISNTILQEELAYLSGKKVRMLCDVQGEVCSPI
RYEMEPFETGKTPDWTLKKIPRYVTQGLFSFNPQCTPDGKVLFWTALVREGGRSTQKVWASKRDQYGFWMAGEQLPSPLN
NRYPSAVISALPGGNELFVFGNFGEEELLDQLKREMTQKSQIATREAANAKEFHIVLTKLENEYKERSEKIQNRAPLYKT
RKTETGWTLPSPIPFPTFYNWYKKADNPNQQVFGGSALSSSGRTLIYSAQQKKNFGKLDLYASIQNESGIFEEGINLGST
LNTGEEEMAPFLGPDDRTLYFSSSGRKEGISIFISRRNGDSWTMWSEPQELSPNLRGVNFFSIPAVGNWAYVSRDGELFM
AAIPHHFQPDPVVVIKGKVVDEEGKALSAFVQYESLLKKKTIGSTISDPNTGEFSIILPYEENYGFYGEKEGYLPVSQNL
NLVGKEKEEKEKTVLLVLPKLKKGNQIVMNNLFFAFRSSEFAKESEPELDRLAGILKKTTNLKVLIEGHTDNVGTKSANQ
KLSLERANSVAFYLKSKHKIEDSRISVVGLGPSNPIAENGSEEGRAVNRRVVFKILEE
>Mature_617_residues
ARILLIILFLFGSGLTSSQSIKNGIQVLEGDLINTGHLLRTEKQVFRISNTILQEELAYLSGKKVRMLCDVQGEVCSPIR
YEMEPFETGKTPDWTLKKIPRYVTQGLFSFNPQCTPDGKVLFWTALVREGGRSTQKVWASKRDQYGFWMAGEQLPSPLNN
RYPSAVISALPGGNELFVFGNFGEEELLDQLKREMTQKSQIATREAANAKEFHIVLTKLENEYKERSEKIQNRAPLYKTR
KTETGWTLPSPIPFPTFYNWYKKADNPNQQVFGGSALSSSGRTLIYSAQQKKNFGKLDLYASIQNESGIFEEGINLGSTL
NTGEEEMAPFLGPDDRTLYFSSSGRKEGISIFISRRNGDSWTMWSEPQELSPNLRGVNFFSIPAVGNWAYVSRDGELFMA
AIPHHFQPDPVVVIKGKVVDEEGKALSAFVQYESLLKKKTIGSTISDPNTGEFSIILPYEENYGFYGEKEGYLPVSQNLN
LVGKEKEEKEKTVLLVLPKLKKGNQIVMNNLFFAFRSSEFAKESEPELDRLAGILKKTTNLKVLIEGHTDNVGTKSANQK
LSLERANSVAFYLKSKHKIEDSRISVVGLGPSNPIAENGSEEGRAVNRRVVFKILEE

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI48994946, Length=113, Percent_Identity=38.0530973451327, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788957, Length=106, Percent_Identity=37.7358490566038, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 69449; Mature: 69318

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARILLIILFLFGSGLTSSQSIKNGIQVLEGDLINTGHLLRTEKQVFRISNTILQEELAY
CHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LSGKKVRMLCDVQGEVCSPIRYEMEPFETGKTPDWTLKKIPRYVTQGLFSFNPQCTPDGK
HCCCCEEEEEECCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
VLFWTALVREGGRSTQKVWASKRDQYGFWMAGEQLPSPLNNRYPSAVISALPGGNELFVF
EEEEEEHHHCCCCHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCHHHHHHCCCCCEEEEE
GNFGEEELLDQLKREMTQKSQIATREAANAKEFHIVLTKLENEYKERSEKIQNRAPLYKT
ECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCEEC
RKTETGWTLPSPIPFPTFYNWYKKADNPNQQVFGGSALSSSGRTLIYSAQQKKNFGKLDL
CCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEECCCHHCCCCCEEEEECHHCCCCCCEEE
YASIQNESGIFEEGINLGSTLNTGEEEMAPFLGPDDRTLYFSSSGRKEGISIFISRRNGD
EEEECCCCCCHHHCCCCCCCCCCCHHHHCCCCCCCCCEEEECCCCCCCCEEEEEEECCCC
SWTMWSEPQELSPNLRGVNFFSIPAVGNWAYVSRDGELFMAAIPHHFQPDPVVVIKGKVV
CCEECCCCHHCCCCCCCCEEEECCCCCCEEEEECCCCEEEEECCCCCCCCCEEEEEEEEE
DEEGKALSAFVQYESLLKKKTIGSTISDPNTGEFSIILPYEENYGFYGEKEGYLPVSQNL
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCEECCCCC
NLVGKEKEEKEKTVLLVLPKLKKGNQIVMNNLFFAFRSSEFAKESEPELDRLAGILKKTT
CEECCCCCCCCCEEEEEECCCCCCCEEEEEHEEEEECCCHHCCCCCCHHHHHHHHHHCCC
NLKVLIEGHTDNVGTKSANQKLSLERANSVAFYLKSKHKIEDSRISVVGLGPSNPIAENG
CEEEEEECCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCEEEEEECCCCCCCCCCC
SEEGRAVNRRVVFKILEE
CCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARILLIILFLFGSGLTSSQSIKNGIQVLEGDLINTGHLLRTEKQVFRISNTILQEELAY
HHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LSGKKVRMLCDVQGEVCSPIRYEMEPFETGKTPDWTLKKIPRYVTQGLFSFNPQCTPDGK
HCCCCEEEEEECCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
VLFWTALVREGGRSTQKVWASKRDQYGFWMAGEQLPSPLNNRYPSAVISALPGGNELFVF
EEEEEEHHHCCCCHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCHHHHHHCCCCCEEEEE
GNFGEEELLDQLKREMTQKSQIATREAANAKEFHIVLTKLENEYKERSEKIQNRAPLYKT
ECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCEEC
RKTETGWTLPSPIPFPTFYNWYKKADNPNQQVFGGSALSSSGRTLIYSAQQKKNFGKLDL
CCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEECCCHHCCCCCEEEEECHHCCCCCCEEE
YASIQNESGIFEEGINLGSTLNTGEEEMAPFLGPDDRTLYFSSSGRKEGISIFISRRNGD
EEEECCCCCCHHHCCCCCCCCCCCHHHHCCCCCCCCCEEEECCCCCCCCEEEEEEECCCC
SWTMWSEPQELSPNLRGVNFFSIPAVGNWAYVSRDGELFMAAIPHHFQPDPVVVIKGKVV
CCEECCCCHHCCCCCCCCEEEECCCCCCEEEEECCCCEEEEECCCCCCCCCEEEEEEEEE
DEEGKALSAFVQYESLLKKKTIGSTISDPNTGEFSIILPYEENYGFYGEKEGYLPVSQNL
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCEECCCCC
NLVGKEKEEKEKTVLLVLPKLKKGNQIVMNNLFFAFRSSEFAKESEPELDRLAGILKKTT
CEECCCCCCCCCEEEEEECCCCCCCEEEEEHEEEEECCCHHCCCCCCHHHHHHHHHHCCC
NLKVLIEGHTDNVGTKSANQKLSLERANSVAFYLKSKHKIEDSRISVVGLGPSNPIAENG
CEEEEEECCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCEEEEEECCCCCCCCCCC
SEEGRAVNRRVVFKILEE
CCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2447060; 10984043 [H]