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The map label for this gene is rsbU [H]

Identifier: 183221998

GI number: 183221998

Start: 2732509

End: 2733753

Strand: Reverse

Name: rsbU [H]

Synonym: LEPBI_I2639

Alternate gene names: 183221998

Gene position: 2733753-2732509 (Counterclockwise)

Preceding gene: 183221999

Following gene: 183221997

Centisome position: 75.94

GC content: 36.22

Gene sequence:

>1245_bases
ATGGGCACTGAAGAAAAACCTACTGTTGATTTAAGTTTCCTTATCAAGGAAATGAAAAAAGTAGAGTTTGGTTCTGGTGA
TCTAGTAATTGAACAATATACGTTAGGTGATTCCTTTTATTTTATTGAAAAGGGATGTGTTGAAGTTTGGAAATACTTAG
ATGAAACCAAACAAGAAATTCTTGTCATTGGAGATTTGGTTGCAGGTGATTATTTTGGTGAAATATCACTCATCGATTCA
GCTCCAAGGACAGTGAATATCACAACCAAAGAAAATTCCATTTTATATAAATTAACAAGAGAAGACTTCCATCGTTTGAT
CCAAACAAGTCCAGAGATGACATTGACTCTTTTAAAATTACTGACCGCAAGGATTCGTAACGCGGAACAAAGAGAAAATC
AAGTCCTCATTCAAAAAAACAAAGAGTTACGTTTGCAAAATGAAACATTAGAGATGATGGTGAAGGAACGGACAGCTAAA
CTCACCCAATCTTTAAAAATCATCCAAGAAGATTTAGAAACTGCCAAAACAATTCAAAGGAATATTTTACCATTAGGATT
GAAGCACATTGCCCATTTGGATTTCGCTTCAAAATTCGAACCGATGGCAGAAGTGGGTGGAGATATCTTTGATGTCATTC
GATTGGATAAATCTAAAATTAGATTGTTTTTAGCAGATGCCATTGGTCACGGTGTCCAGGCGGCTCTTATCACCATGGCC
ATCAAAGCTGAGTATGACCATTTAAAACACAATACACAAAATCCTTCTGATGTATTGGTTGCGCTAAACCAAATTTTTAT
CAATCGTTACGGGAAAAAATCCAATCAGTTTACGGCAGTGATTGTTGATTTAAAACTAGAGGAAAATACACTCGTTTATT
CTTCTGCAGGTCATAACGAACCTTATCTTTTGAATCAAACGGAAATTTTGCCGTTAAATGAGTCGGGGTTAATGCTTGGC
CTTGAAAAAGACGTAGAATATTCCAATACCTTGGTACCATTTGGACTTGGGGATCGATTGTTTATGATTTCTGACGGGTA
TTTAGAACAAGAAAATGAGAAAGGAATTTTACTTGGTGAATCCAAATTGCTTTCCAGTTTTCACTCTGCCAGGAGTTTAG
GAATAAGTTTAGCTGATACCGTGCATTATCTTATGGAAGAATTCCATACCTTTCGGGGGAATACCCCTATGATGGATGAT
GTTACTATTTTGGGAATCGGTACCTCGTACGAATTAGGAGTTTAA

Upstream 100 bases:

>100_bases
CTCTAATCCTTTTACAGAAGATGCAGAATATTGGTCTATTTGTAAACCAGCATTCACTTTAGATGCAGTTAACCGCGAAG
AAGAATAATCCACCAAATTT

Downstream 100 bases:

>100_bases
ATTGGGTTTAATCATCGCATACATCTTGTTTCTTTTGAATTTACTTTCGATCATTCCTACAATGTATCCATTGTATATAT
GGAAACTTTTAACAACAGGT

Product: putative serine phosphatase

Products: NA

Alternate protein names: Sigma factor sigB regulation protein rsbU [H]

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MGTEEKPTVDLSFLIKEMKKVEFGSGDLVIEQYTLGDSFYFIEKGCVEVWKYLDETKQEILVIGDLVAGDYFGEISLIDS
APRTVNITTKENSILYKLTREDFHRLIQTSPEMTLTLLKLLTARIRNAEQRENQVLIQKNKELRLQNETLEMMVKERTAK
LTQSLKIIQEDLETAKTIQRNILPLGLKHIAHLDFASKFEPMAEVGGDIFDVIRLDKSKIRLFLADAIGHGVQAALITMA
IKAEYDHLKHNTQNPSDVLVALNQIFINRYGKKSNQFTAVIVDLKLEENTLVYSSAGHNEPYLLNQTEILPLNESGLMLG
LEKDVEYSNTLVPFGLGDRLFMISDGYLEQENEKGILLGESKLLSSFHSARSLGISLADTVHYLMEEFHTFRGNTPMMDD
VTILGIGTSYELGV

Sequences:

>Translated_414_residues
MGTEEKPTVDLSFLIKEMKKVEFGSGDLVIEQYTLGDSFYFIEKGCVEVWKYLDETKQEILVIGDLVAGDYFGEISLIDS
APRTVNITTKENSILYKLTREDFHRLIQTSPEMTLTLLKLLTARIRNAEQRENQVLIQKNKELRLQNETLEMMVKERTAK
LTQSLKIIQEDLETAKTIQRNILPLGLKHIAHLDFASKFEPMAEVGGDIFDVIRLDKSKIRLFLADAIGHGVQAALITMA
IKAEYDHLKHNTQNPSDVLVALNQIFINRYGKKSNQFTAVIVDLKLEENTLVYSSAGHNEPYLLNQTEILPLNESGLMLG
LEKDVEYSNTLVPFGLGDRLFMISDGYLEQENEKGILLGESKLLSSFHSARSLGISLADTVHYLMEEFHTFRGNTPMMDD
VTILGIGTSYELGV
>Mature_413_residues
GTEEKPTVDLSFLIKEMKKVEFGSGDLVIEQYTLGDSFYFIEKGCVEVWKYLDETKQEILVIGDLVAGDYFGEISLIDSA
PRTVNITTKENSILYKLTREDFHRLIQTSPEMTLTLLKLLTARIRNAEQRENQVLIQKNKELRLQNETLEMMVKERTAKL
TQSLKIIQEDLETAKTIQRNILPLGLKHIAHLDFASKFEPMAEVGGDIFDVIRLDKSKIRLFLADAIGHGVQAALITMAI
KAEYDHLKHNTQNPSDVLVALNQIFINRYGKKSNQFTAVIVDLKLEENTLVYSSAGHNEPYLLNQTEILPLNESGLMLGL
EKDVEYSNTLVPFGLGDRLFMISDGYLEQENEKGILLGESKLLSSFHSARSLGISLADTVHYLMEEFHTFRGNTPMMDDV
TILGIGTSYELGV

Specific function: Positive regulator of sigma-B activity. Dephosphorylates rsbV in response to environmental stress conveyed from the rsbXST module [H]

COG id: COG2208

COG function: function code TK; Serine phosphatase RsbU, regulator of sigma subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PP2C-like domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017944
- InterPro:   IPR001932
- InterPro:   IPR014787
- InterPro:   IPR010822
- ProDom:   PD685490 [H]

Pfam domain/function: PF08673 RsbU_N; PF07228 SpoIIE [H]

EC number: =3.1.3.3 [H]

Molecular weight: Translated: 46785; Mature: 46654

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50042 CNMP_BINDING_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTEEKPTVDLSFLIKEMKKVEFGSGDLVIEQYTLGDSFYFIEKGCVEVWKYLDETKQEI
CCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEHHHHHHHHHHHHHHHHHHE
LVIGDLVAGDYFGEISLIDSAPRTVNITTKENSILYKLTREDFHRLIQTSPEMTLTLLKL
EEEEEEHHCCCCCEEEEECCCCCEEEEEECCCCEEEEECHHHHHHHHHCCCHHHHHHHHH
LTARIRNAEQRENQVLIQKNKELRLQNETLEMMVKERTAKLTQSLKIIQEDLETAKTIQR
HHHHHHCCHHHCCEEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NILPLGLKHIAHLDFASKFEPMAEVGGDIFDVIRLDKSKIRLFLADAIGHGVQAALITMA
CCCHHHHHHHHHHCHHHHCCCHHHHCCHHHHHHHCCHHHHEEEEEHHHCCCHHHHHHHHH
IKAEYDHLKHNTQNPSDVLVALNQIFINRYGKKSNQFTAVIVDLKLEENTLVYSSAGHNE
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCEEEEECCCCCC
PYLLNQTEILPLNESGLMLGLEKDVEYSNTLVPFGLGDRLFMISDGYLEQENEKGILLGE
CEEECCCEEEEECCCCEEEEEECCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCEEEEH
SKLLSSFHSARSLGISLADTVHYLMEEFHTFRGNTPMMDDVTILGIGTSYELGV
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure 
GTEEKPTVDLSFLIKEMKKVEFGSGDLVIEQYTLGDSFYFIEKGCVEVWKYLDETKQEI
CCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEHHHHHHHHHHHHHHHHHHE
LVIGDLVAGDYFGEISLIDSAPRTVNITTKENSILYKLTREDFHRLIQTSPEMTLTLLKL
EEEEEEHHCCCCCEEEEECCCCCEEEEEECCCCEEEEECHHHHHHHHHCCCHHHHHHHHH
LTARIRNAEQRENQVLIQKNKELRLQNETLEMMVKERTAKLTQSLKIIQEDLETAKTIQR
HHHHHHCCHHHCCEEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NILPLGLKHIAHLDFASKFEPMAEVGGDIFDVIRLDKSKIRLFLADAIGHGVQAALITMA
CCCHHHHHHHHHHCHHHHCCCHHHHCCHHHHHHHCCHHHHEEEEEHHHCCCHHHHHHHHH
IKAEYDHLKHNTQNPSDVLVALNQIFINRYGKKSNQFTAVIVDLKLEENTLVYSSAGHNE
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCEEEEECCCCCC
PYLLNQTEILPLNESGLMLGLEKDVEYSNTLVPFGLGDRLFMISDGYLEQENEKGILLGE
CEEECCCEEEEECCCCEEEEEECCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCEEEEH
SKLLSSFHSARSLGISLADTVHYLMEEFHTFRGNTPMMDDVTILGIGTSYELGV
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8002610; 8002609; 9384377; 8824586; 9786195; 12499568 [H]