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The map label for this gene is tpsA [H]

Identifier: 183221978

GI number: 183221978

Start: 2708807

End: 2710999

Strand: Direct

Name: tpsA [H]

Synonym: LEPBI_I2619

Alternate gene names: 183221978

Gene position: 2708807-2710999 (Clockwise)

Preceding gene: 183221962

Following gene: 183221979

Centisome position: 75.25

GC content: 38.44

Gene sequence:

>2193_bases
ATGAGACTGATCCTTGTATCCAACAGGTTACCAGTCCAATGGGACGGAACACCCAATGTCGGTGGACTTGCCACTGGTCT
TTCTAGTTTTCTTTCCCGTTGGAAATCACTTGGAAATGAAGTGGTTTGGGTGGGCTGGCCTGGTAAATCCATCCCAGAAA
AAGAACAACGAAACTATTCCAAAAAAATGTTGGAGGAACATGGAACAATCCCAGTGTTTCTAAAACAAAAGTTAGCCGAT
TCTTTTTACAATGGCTTTTGTAACAAAACCATTTGGCCCCTCTTCCATTATTTCACTGCTAATTGTGAGTTTTCTGATTT
AACCTTTGCCTCTTATGAAGAAGCAAACCAAGAATTTGCAAAAGCCATAAAAGAAATTTACCAACCTGGAGATTGGGTTT
GGGTGCATGATTACCATCTATTCCTTCTCCCTGGAATCTTAAGGAGTGAATTCCCAAATATCCTTTTATCTTTTTTCTTA
CACATACCCTTCCCTACCTTTGAAGTCTTTCGTTTACTCCCAGAACGAATCCGAAGCAAAATACTCGAAGGAGTTTTGGG
TGCAGATCTCATTGGATTTCATACACAAAGTTACACTCAATATTTCTTACGATCCCTACTTCGCTGTTTAGGAATTGAAA
ATGAAAGAGGTATCATATACTTTCAAAACCACCTAACTAAGGCAGCTGCTTTCCCTATGGGTATCAATGTAGAACAATTC
TCCAAGTATGCAAACTCAGACGAATGTGAAACCATCGCAAACCAAATCAATGTGAACCACAAAAATCTGAAACAAATCCT
TTCCGTTGACAGATTGGATTATACAAAAGGAGTCTTACAAAGATTAAACGCATTCGAATTGTTTTTAAAAAATAACCCCC
AATGGATCAAAAAATGTAAACTTGTTTTGGTTCTTGTCCCCTCTCGAACAGACGTATCCTCCTACCAATCCATGAAACGT
GCCATCGATGAAAAGATAGGTGCCATCAATGGGAGTTTTGGTACGTTAGATTGGACACCTATTTTGTATCAGTACAAAGG
ATTTCCATTTGAAGAACTAGTTCCGTTATATAAAAACACTCATGTTATGTTAGTCACACCTTTTCGAGATGGAATGAATT
TAGTGGCAAAAGAATTTTTGGTGTCACAAAAAAATGGGATGTTGGTACTAAGTGAGATGGCTGGTGCGTCTGCAGAATTA
CCAGAAGCCATCCTAGTGAATCCCAATGATTTGAGTGGGATGGCAGATGCAATCAAAGAAGCCATCGAGATGGAAGAATC
AGAAATTAATATCCGTAACCAAGTGATGGTTTCTCGTTTAAAAGAGAATTCAGTCATGGAGTGGGCAAAAAAAATCTTTA
ACGAAACAGAAGAGACAGCAAATCAAAACTTAAACTTCCAAACAAAATCGATTCCCCCTCACTCTGATTCGTTACTTCCG
AAATCCAATTCTCCAATTTTCATTTTGTTTGATTATGATGGCACATTGGTTCCATTCCAGTCCTTCCCTCATTTAGCAGT
CCCTTCCAAGGAACTTGTGAATTCCCTCACTCAACTATCAAAGTTTTCCAATGTCTCGGTTGCGATCATCAGCGGAAGGG
ATCGCAAATTTTTAGAATCAATATTTTTTGACATGCCCTTCCATTTAGTAGCAGAACATGGAGCTTGGCACCGAGCTCCC
AATCAAACAGAATGGCAGGCATTATTTTTTTCCAATGCGGAATGGAAAAAAGAAATCAAATCACATTTAGAAGAATTTTC
GAAACGAGTTCCAGGTTCCTTCACAGAGGAAAAAGAATTTTCGCTCGTTTGGCATTTTAGAAATGCAGATCCTGATATAG
GATTAAATGCCGCAAGAGAAATGTTAGACGAACTATCCCAAATATCATCAAACAGCGGTTTTTTTGTCCAAAGAGGAAAC
AAAATCATAGAAGTGAGAGAATACGGAACTGGAAAAGGGAAAGCTGCTTTAAAAATCCTTCCCAACACTTCGATTCAAAC
CTTTGTTTTTGGAGATGACACAACTGACGAAGATATGTTTCGCGAGTTGCCAGATCATGCCATCAGCGTCAAAATTGGGA
AATCAGAAACCATCGCAAAATTCCGATTCCAATCAACAGATGATGTTCATCTATGGATACAAAATTTAATCAAACACTTA
ACAAAGGTTCATCATGACGTTACACAAATATAA

Upstream 100 bases:

>100_bases
TAAGTCTAATGAGTTTATAGAAAGAATATTAGATATTTCAAATATCATTTTTTTCAAAGAAACCCCTTGCCATATAGTTT
TCATCTGTTCTCATTGAAAC

Downstream 100 bases:

>100_bases
CACAGGGATTATAGGAAACGGAAGTTACATCGCACATATCAACACTCATGCAGAAGTTGTTTGGATGTGTTGGCCATATT
TTGATAGTTCTCCTATTTTT

Product: putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB

Products: H+; alpha,alpha'-Trehalose 6-phosphate; UDP [C]

Alternate protein names: TPS; Trehalose-6-phosphate synthase [H]

Number of amino acids: Translated: 730; Mature: 730

Protein sequence:

>730_residues
MRLILVSNRLPVQWDGTPNVGGLATGLSSFLSRWKSLGNEVVWVGWPGKSIPEKEQRNYSKKMLEEHGTIPVFLKQKLAD
SFYNGFCNKTIWPLFHYFTANCEFSDLTFASYEEANQEFAKAIKEIYQPGDWVWVHDYHLFLLPGILRSEFPNILLSFFL
HIPFPTFEVFRLLPERIRSKILEGVLGADLIGFHTQSYTQYFLRSLLRCLGIENERGIIYFQNHLTKAAAFPMGINVEQF
SKYANSDECETIANQINVNHKNLKQILSVDRLDYTKGVLQRLNAFELFLKNNPQWIKKCKLVLVLVPSRTDVSSYQSMKR
AIDEKIGAINGSFGTLDWTPILYQYKGFPFEELVPLYKNTHVMLVTPFRDGMNLVAKEFLVSQKNGMLVLSEMAGASAEL
PEAILVNPNDLSGMADAIKEAIEMEESEINIRNQVMVSRLKENSVMEWAKKIFNETEETANQNLNFQTKSIPPHSDSLLP
KSNSPIFILFDYDGTLVPFQSFPHLAVPSKELVNSLTQLSKFSNVSVAIISGRDRKFLESIFFDMPFHLVAEHGAWHRAP
NQTEWQALFFSNAEWKKEIKSHLEEFSKRVPGSFTEEKEFSLVWHFRNADPDIGLNAAREMLDELSQISSNSGFFVQRGN
KIIEVREYGTGKGKAALKILPNTSIQTFVFGDDTTDEDMFRELPDHAISVKIGKSETIAKFRFQSTDDVHLWIQNLIKHL
TKVHHDVTQI

Sequences:

>Translated_730_residues
MRLILVSNRLPVQWDGTPNVGGLATGLSSFLSRWKSLGNEVVWVGWPGKSIPEKEQRNYSKKMLEEHGTIPVFLKQKLAD
SFYNGFCNKTIWPLFHYFTANCEFSDLTFASYEEANQEFAKAIKEIYQPGDWVWVHDYHLFLLPGILRSEFPNILLSFFL
HIPFPTFEVFRLLPERIRSKILEGVLGADLIGFHTQSYTQYFLRSLLRCLGIENERGIIYFQNHLTKAAAFPMGINVEQF
SKYANSDECETIANQINVNHKNLKQILSVDRLDYTKGVLQRLNAFELFLKNNPQWIKKCKLVLVLVPSRTDVSSYQSMKR
AIDEKIGAINGSFGTLDWTPILYQYKGFPFEELVPLYKNTHVMLVTPFRDGMNLVAKEFLVSQKNGMLVLSEMAGASAEL
PEAILVNPNDLSGMADAIKEAIEMEESEINIRNQVMVSRLKENSVMEWAKKIFNETEETANQNLNFQTKSIPPHSDSLLP
KSNSPIFILFDYDGTLVPFQSFPHLAVPSKELVNSLTQLSKFSNVSVAIISGRDRKFLESIFFDMPFHLVAEHGAWHRAP
NQTEWQALFFSNAEWKKEIKSHLEEFSKRVPGSFTEEKEFSLVWHFRNADPDIGLNAAREMLDELSQISSNSGFFVQRGN
KIIEVREYGTGKGKAALKILPNTSIQTFVFGDDTTDEDMFRELPDHAISVKIGKSETIAKFRFQSTDDVHLWIQNLIKHL
TKVHHDVTQI
>Mature_730_residues
MRLILVSNRLPVQWDGTPNVGGLATGLSSFLSRWKSLGNEVVWVGWPGKSIPEKEQRNYSKKMLEEHGTIPVFLKQKLAD
SFYNGFCNKTIWPLFHYFTANCEFSDLTFASYEEANQEFAKAIKEIYQPGDWVWVHDYHLFLLPGILRSEFPNILLSFFL
HIPFPTFEVFRLLPERIRSKILEGVLGADLIGFHTQSYTQYFLRSLLRCLGIENERGIIYFQNHLTKAAAFPMGINVEQF
SKYANSDECETIANQINVNHKNLKQILSVDRLDYTKGVLQRLNAFELFLKNNPQWIKKCKLVLVLVPSRTDVSSYQSMKR
AIDEKIGAINGSFGTLDWTPILYQYKGFPFEELVPLYKNTHVMLVTPFRDGMNLVAKEFLVSQKNGMLVLSEMAGASAEL
PEAILVNPNDLSGMADAIKEAIEMEESEINIRNQVMVSRLKENSVMEWAKKIFNETEETANQNLNFQTKSIPPHSDSLLP
KSNSPIFILFDYDGTLVPFQSFPHLAVPSKELVNSLTQLSKFSNVSVAIISGRDRKFLESIFFDMPFHLVAEHGAWHRAP
NQTEWQALFFSNAEWKKEIKSHLEEFSKRVPGSFTEEKEFSLVWHFRNADPDIGLNAAREMLDELSQISSNSGFFVQRGN
KIIEVREYGTGKGKAALKILPNTSIQTFVFGDDTTDEDMFRELPDHAISVKIGKSETIAKFRFQSTDDVHLWIQNLIKHL
TKVHHDVTQI

Specific function: Catalyzes the transfer of glucose from a nucleoside diphosphate-glucose to glucose-6-phosphate to form trehalose-6- phosphate and nucleoside diphosphate [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=477, Percent_Identity=33.3333333333333, Blast_Score=248, Evalue=8e-67,
Organism=Escherichia coli, GI1788207, Length=237, Percent_Identity=28.2700421940928, Blast_Score=79, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI115533558, Length=359, Percent_Identity=29.2479108635098, Blast_Score=151, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI115533560, Length=359, Percent_Identity=29.2479108635098, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71987012, Length=361, Percent_Identity=29.6398891966759, Blast_Score=149, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6319602, Length=469, Percent_Identity=38.8059701492537, Blast_Score=354, Evalue=3e-98,
Organism=Saccharomyces cerevisiae, GI6320279, Length=667, Percent_Identity=31.784107946027, Blast_Score=301, Evalue=2e-82,
Organism=Saccharomyces cerevisiae, GI6323917, Length=589, Percent_Identity=27.3344651952462, Blast_Score=233, Evalue=7e-62,
Organism=Saccharomyces cerevisiae, GI6323537, Length=666, Percent_Identity=25.3753753753754, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI19920676, Length=772, Percent_Identity=33.8082901554404, Blast_Score=360, Evalue=2e-99,
Organism=Drosophila melanogaster, GI221473368, Length=251, Percent_Identity=28.6852589641434, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI20129307, Length=251, Percent_Identity=28.6852589641434, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24582490, Length=251, Percent_Identity=28.6852589641434, Blast_Score=78, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: 2.4.1.15 [C]

Molecular weight: Translated: 83582; Mature: 83582

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLILVSNRLPVQWDGTPNVGGLATGLSSFLSRWKSLGNEVVWVGWPGKSIPEKEQRNYS
CEEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHH
KKMLEEHGTIPVFLKQKLADSFYNGFCNKTIWPLFHYFTANCEFSDLTFASYEEANQEFA
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
KAIKEIYQPGDWVWVHDYHLFLLPGILRSEFPNILLSFFLHIPFPTFEVFRLLPERIRSK
HHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
ILEGVLGADLIGFHTQSYTQYFLRSLLRCLGIENERGIIYFQNHLTKAAAFPMGINVEQF
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHCCCCCCCHHHH
SKYANSDECETIANQINVNHKNLKQILSVDRLDYTKGVLQRLNAFELFLKNNPQWIKKCK
HHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCE
LVLVLVPSRTDVSSYQSMKRAIDEKIGAINGSFGTLDWTPILYQYKGFPFEELVPLYKNT
EEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHEECCCCHHHHHHHHCCC
HVMLVTPFRDGMNLVAKEFLVSQKNGMLVLSEMAGASAELPEAILVNPNDLSGMADAIKE
EEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHH
AIEMEESEINIRNQVMVSRLKENSVMEWAKKIFNETEETANQNLNFQTKSIPPHSDSLLP
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCC
KSNSPIFILFDYDGTLVPFQSFPHLAVPSKELVNSLTQLSKFSNVSVAIISGRDRKFLES
CCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHH
IFFDMPFHLVAEHGAWHRAPNQTEWQALFFSNAEWKKEIKSHLEEFSKRVPGSFTEEKEF
HHHCCCHHHHHCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCE
SLVWHFRNADPDIGLNAAREMLDELSQISSNSGFFVQRGNKIIEVREYGTGKGKAALKIL
EEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEEECCCCCCCEEEEEE
PNTSIQTFVFGDDTTDEDMFRELPDHAISVKIGKSETIAKFRFQSTDDVHLWIQNLIKHL
CCCCEEEEEECCCCCCHHHHHHCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHH
TKVHHDVTQI
HHHHHHHHCC
>Mature Secondary Structure
MRLILVSNRLPVQWDGTPNVGGLATGLSSFLSRWKSLGNEVVWVGWPGKSIPEKEQRNYS
CEEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHH
KKMLEEHGTIPVFLKQKLADSFYNGFCNKTIWPLFHYFTANCEFSDLTFASYEEANQEFA
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
KAIKEIYQPGDWVWVHDYHLFLLPGILRSEFPNILLSFFLHIPFPTFEVFRLLPERIRSK
HHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
ILEGVLGADLIGFHTQSYTQYFLRSLLRCLGIENERGIIYFQNHLTKAAAFPMGINVEQF
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHCCCCCCCHHHH
SKYANSDECETIANQINVNHKNLKQILSVDRLDYTKGVLQRLNAFELFLKNNPQWIKKCK
HHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCE
LVLVLVPSRTDVSSYQSMKRAIDEKIGAINGSFGTLDWTPILYQYKGFPFEELVPLYKNT
EEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHEECCCCHHHHHHHHCCC
HVMLVTPFRDGMNLVAKEFLVSQKNGMLVLSEMAGASAELPEAILVNPNDLSGMADAIKE
EEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHH
AIEMEESEINIRNQVMVSRLKENSVMEWAKKIFNETEETANQNLNFQTKSIPPHSDSLLP
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCC
KSNSPIFILFDYDGTLVPFQSFPHLAVPSKELVNSLTQLSKFSNVSVAIISGRDRKFLES
CCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHH
IFFDMPFHLVAEHGAWHRAPNQTEWQALFFSNAEWKKEIKSHLEEFSKRVPGSFTEEKEF
HHHCCCHHHHHCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCE
SLVWHFRNADPDIGLNAAREMLDELSQISSNSGFFVQRGNKIIEVREYGTGKGKAALKIL
EEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEEECCCCCCCEEEEEE
PNTSIQTFVFGDDTTDEDMFRELPDHAISVKIGKSETIAKFRFQSTDDVHLWIQNLIKHL
CCCCEEEEEECCCCCCHHHHHHCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHH
TKVHHDVTQI
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Monovalent cations [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-Glucose 6-phosphate; UDPglucose [C]

Specific reaction: D-Glucose 6-phosphate + UDPglucose --> H+ + alpha,alpha'-Trehalose 6-phosphate + UDP [C]

General reaction: Hexosyl group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA