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The map label for this gene is aroA

Identifier: 183221957

GI number: 183221957

Start: 2685297

End: 2686583

Strand: Reverse

Name: aroA

Synonym: LEPBI_I2598

Alternate gene names: 183221957

Gene position: 2686583-2685297 (Counterclockwise)

Preceding gene: 183221960

Following gene: 183221955

Centisome position: 74.63

GC content: 42.89

Gene sequence:

>1287_bases
ATGTTACAGCCACAAATCAAATTAAATGCGAAAAATGAAATTTATGTCCCTGGAGATAAGTCCATATCACATCGGACCGT
ACTATTTTGTGCACTCTCCCAAGGGAAATCCGAGATTCATGGCTTTTTAGAAGGAGAAGACCCTCTCCACACCTTACGAT
GTTTTGAATCTATGGGACTTTCTGTCTCATCCCTTGGAAAAGGGAGTTATTCTGTTGTCAGTCCTGGCAAACAAAATCTC
AATTCTCCTAAGGGAGTTTTGGATTTTGGGAATGCTGGCACGGGGATTCGACTATCCGCAGGCCTACTGGCAGGCCTTCC
TGGGATGAACGCCACACTCACGGGTGACGCCTCCCTTTGCAAACGACCGATGGCAAGGATCATGAATCCTTTGCAAGAGA
TGGGAGCAAGTGTCATCTCCGTTGAAGGGAATGATCGAGCCCCGCTTCGCATCGAAGGGAAACAATTAAAAGACTATTCT
TACGTAAGTCCCATTGCTTCGGCACAAATCAAAAGTGCCCTTGTCCTTGCGGCACTTGCATCGGATATCTCCATTGAGTA
CAAAGAATCCGAAGTTTCAAGAGACCATACAGAGAATATGATTCGTTTCCTTGGGGGAACGATTACCCACCATTCCTCGG
TACATTTTACGGTAAAACCACCATATCATTTCGAAGGAACCAAATATGTAATCCCAGGGGATATTTCCAGCGCGGCCTTT
TTTATTGTTTTTGGACTTTGTGTGGGGGGGAGTGAACCCCTTCTCATCAAAAACATTGGTCTCAATCCTTCCAGGATTGG
GATCCTCACTGTTTTACAAAACATGGGCGGGAAAATTGAAATTATCGCCAAACGCGTAGAATGTGGTGAAGAGATTGGTG
ATTTACTCGTTTATCCATCCAAACTCAAACGAACTGTCATTACCGAAGACTTAATCCCTTCCATTATCGATGAAATTCCC
ATCTTAACGATTGCAGGTCTTTTTTCAGAGGGAGGATTTCAAATTTCCCATGCGGAAGAGCTCCGAGCAAAAGAATCAGA
CAGAATCCGTTCCATGGTATCCAATTTAGAAAGATTGGGTGTGAAAGTGAAGGAAGTAAATGATGGATATGAATTTGATG
AAGTAGGAACCATTCAAAATGCAAAAATTGAAACCTTTATGGACCATCGCATTGCGATGAGTTTTGCGATCCTATCCAAA
TTATCAGGCGTTTCACTTTCTTTTGACGATACAAGTTGGGTGGATACAAGTTTCCCTGGATTTTTTGAAATTCTAAAGTC
TGTTTGA

Upstream 100 bases:

>100_bases
TATCTAGAGGCATTTGGTTGTTCGTAAAGGAAACTTAGGTTTCCGACAAACGCTAATAAAGAAGCGCCAAATTGCAAAAT
ACTTTTTAGAAAAATACAAC

Downstream 100 bases:

>100_bases
ATTGTGATTGGATAAAGTTAGTGGAAATTTGAATTGGTACTCGGCACCTTTCTCTCCATTAAAAGATAGATTACCTTTAA
ATTGTTTGGTTAACATTTTT

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MLQPQIKLNAKNEIYVPGDKSISHRTVLFCALSQGKSEIHGFLEGEDPLHTLRCFESMGLSVSSLGKGSYSVVSPGKQNL
NSPKGVLDFGNAGTGIRLSAGLLAGLPGMNATLTGDASLCKRPMARIMNPLQEMGASVISVEGNDRAPLRIEGKQLKDYS
YVSPIASAQIKSALVLAALASDISIEYKESEVSRDHTENMIRFLGGTITHHSSVHFTVKPPYHFEGTKYVIPGDISSAAF
FIVFGLCVGGSEPLLIKNIGLNPSRIGILTVLQNMGGKIEIIAKRVECGEEIGDLLVYPSKLKRTVITEDLIPSIIDEIP
ILTIAGLFSEGGFQISHAEELRAKESDRIRSMVSNLERLGVKVKEVNDGYEFDEVGTIQNAKIETFMDHRIAMSFAILSK
LSGVSLSFDDTSWVDTSFPGFFEILKSV

Sequences:

>Translated_428_residues
MLQPQIKLNAKNEIYVPGDKSISHRTVLFCALSQGKSEIHGFLEGEDPLHTLRCFESMGLSVSSLGKGSYSVVSPGKQNL
NSPKGVLDFGNAGTGIRLSAGLLAGLPGMNATLTGDASLCKRPMARIMNPLQEMGASVISVEGNDRAPLRIEGKQLKDYS
YVSPIASAQIKSALVLAALASDISIEYKESEVSRDHTENMIRFLGGTITHHSSVHFTVKPPYHFEGTKYVIPGDISSAAF
FIVFGLCVGGSEPLLIKNIGLNPSRIGILTVLQNMGGKIEIIAKRVECGEEIGDLLVYPSKLKRTVITEDLIPSIIDEIP
ILTIAGLFSEGGFQISHAEELRAKESDRIRSMVSNLERLGVKVKEVNDGYEFDEVGTIQNAKIETFMDHRIAMSFAILSK
LSGVSLSFDDTSWVDTSFPGFFEILKSV
>Mature_428_residues
MLQPQIKLNAKNEIYVPGDKSISHRTVLFCALSQGKSEIHGFLEGEDPLHTLRCFESMGLSVSSLGKGSYSVVSPGKQNL
NSPKGVLDFGNAGTGIRLSAGLLAGLPGMNATLTGDASLCKRPMARIMNPLQEMGASVISVEGNDRAPLRIEGKQLKDYS
YVSPIASAQIKSALVLAALASDISIEYKESEVSRDHTENMIRFLGGTITHHSSVHFTVKPPYHFEGTKYVIPGDISSAAF
FIVFGLCVGGSEPLLIKNIGLNPSRIGILTVLQNMGGKIEIIAKRVECGEEIGDLLVYPSKLKRTVITEDLIPSIIDEIP
ILTIAGLFSEGGFQISHAEELRAKESDRIRSMVSNLERLGVKVKEVNDGYEFDEVGTIQNAKIETFMDHRIAMSFAILSK
LSGVSLSFDDTSWVDTSFPGFFEILKSV

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family

Homologues:

Organism=Escherichia coli, GI1787137, Length=438, Percent_Identity=28.7671232876712, Blast_Score=136, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6320332, Length=458, Percent_Identity=25.5458515283843, Blast_Score=103, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROA_LEPBA (B0SDL8)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001963580.1
- ProteinModelPortal:   B0SDL8
- SMR:   B0SDL8
- GeneID:   6389610
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_2518
- HOGENOM:   HBG646626
- OMA:   LSRNHTE
- ProtClustDB:   PRK02427
- BioCyc:   LBIF355278:LBF_2518-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00210
- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.10
- PIRSF:   PIRSF000505
- TIGRFAMs:   TIGR01356

Pfam domain/function: PF00275 EPSP_synthase; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =2.5.1.19

Molecular weight: Translated: 46410; Mature: 46410

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00104 EPSP_SYNTHASE_1; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQPQIKLNAKNEIYVPGDKSISHRTVLFCALSQGKSEIHGFLEGEDPLHTLRCFESMGL
CCCCCEEECCCCEEEECCCCCCCCEEEEEEEECCCHHHHCEECCCCCHHHHHHHHHHHCC
SVSSLGKGSYSVVSPGKQNLNSPKGVLDFGNAGTGIRLSAGLLAGLPGMNATLTGDASLC
CHHHCCCCCEEEECCCHHCCCCCCCEEECCCCCCCEEEECHHHHCCCCCCEEECCCHHHH
KRPMARIMNPLQEMGASVISVEGNDRAPLRIEGKQLKDYSYVSPIASAQIKSALVLAALA
HHHHHHHHHHHHHCCCEEEEECCCCCCCEEECCCCCCCCHHHCHHHHHHHHHHHHHHHHH
SDISIEYKESEVSRDHTENMIRFLGGTITHHSSVHFTVKPPYHFEGTKYVIPGDISSAAF
HCCEEEECHHHHHHHHHHHHHHHHCCCEEECCEEEEEECCCCEECCEEEEECCCCCHHHH
FIVFGLCVGGSEPLLIKNIGLNPSRIGILTVLQNMGGKIEIIAKRVECGEEIGDLLVYPS
HHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHEECHH
KLKRTVITEDLIPSIIDEIPILTIAGLFSEGGFQISHAEELRAKESDRIRSMVSNLERLG
HHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHC
VKVKEVNDGYEFDEVGTIQNAKIETFMDHRIAMSFAILSKLSGVSLSFDDTSWVDTSFPG
CEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCH
FFEILKSV
HHHHHHCC
>Mature Secondary Structure
MLQPQIKLNAKNEIYVPGDKSISHRTVLFCALSQGKSEIHGFLEGEDPLHTLRCFESMGL
CCCCCEEECCCCEEEECCCCCCCCEEEEEEEECCCHHHHCEECCCCCHHHHHHHHHHHCC
SVSSLGKGSYSVVSPGKQNLNSPKGVLDFGNAGTGIRLSAGLLAGLPGMNATLTGDASLC
CHHHCCCCCEEEECCCHHCCCCCCCEEECCCCCCCEEEECHHHHCCCCCCEEECCCHHHH
KRPMARIMNPLQEMGASVISVEGNDRAPLRIEGKQLKDYSYVSPIASAQIKSALVLAALA
HHHHHHHHHHHHHCCCEEEEECCCCCCCEEECCCCCCCCHHHCHHHHHHHHHHHHHHHHH
SDISIEYKESEVSRDHTENMIRFLGGTITHHSSVHFTVKPPYHFEGTKYVIPGDISSAAF
HCCEEEECHHHHHHHHHHHHHHHHCCCEEECCEEEEEECCCCEECCEEEEECCCCCHHHH
FIVFGLCVGGSEPLLIKNIGLNPSRIGILTVLQNMGGKIEIIAKRVECGEEIGDLLVYPS
HHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHEECHH
KLKRTVITEDLIPSIIDEIPILTIAGLFSEGGFQISHAEELRAKESDRIRSMVSNLERLG
HHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHC
VKVKEVNDGYEFDEVGTIQNAKIETFMDHRIAMSFAILSKLSGVSLSFDDTSWVDTSFPG
CEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCH
FFEILKSV
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA