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The map label for this gene is argF [H]

Identifier: 183221801

GI number: 183221801

Start: 2505716

End: 2506648

Strand: Direct

Name: argF [H]

Synonym: LEPBI_I2432

Alternate gene names: 183221801

Gene position: 2505716-2506648 (Clockwise)

Preceding gene: 183221800

Following gene: 183221803

Centisome position: 69.61

GC content: 38.69

Gene sequence:

>933_bases
ATGTCCCAAGTGAAACATTTAATCTCCTGGAAGGATTGGACCGATGGAGAAATCCAAGAACTCCTAGATTTTGCAGTCTA
TGTAAAGAAAAATCGAGTGTATTTTTCTGGGCATATGTCTGGCAGATCTCTTGCAATGTTATTTCAAAAAACATCGACAA
GAACACGAGTTTCCTTTGAAGCAGGAATGACAGAGCTCGGTGGACACGCCATATTTTTGGATTGGATGGCATCTAATTTT
TTACTTTCAGACATTGACTTTGAAGGAAAATACCTTTCGAGTAACGTTGCCATCATTATGGCTCGTCTCAAAAAACATGA
AGATTTGCTTGTATTGAAGTCAGGATCTACTGTTCCCGTCATCAATGGTTGCTGTAATTTGTTCCATCCATGCCAGTCAT
TAGCTGACATACTAACAATAGTTATGGACTCTCCCAATGATTGGAAAAAGAAACAAGTATGTTATATTGGTGTTCACAAT
AATGTGGCCAACTCGTTGGTGGAAATCACCGCAGCACTAGGTATCCATTTGATTTTAGTAACGCCCATTGCTGCAAAAGA
ATCCATTGTGGCTGAAGCCATCGATAGAGCACAGAAAAAAGGAACTGTCTCTTGGGAAATGGATGTTCATAAAGCTGTTT
CGCATGCAGATTATGTTTATACTGATACTTGGGTTGATATGGAATTTTTTAACGACCCAAAATACCAAAAAGAAAAAGAA
GAAAGAATTCAAATGATGATGCCCTACCAAGTGAATGCCGATCTGATGAAACATTCCAAAGCAAAAGTCATGCATGATAT
GCCAATCCACGCCGGTTATGAGATCACAAGAGAAATGGTAGAAAGCGATCGTTCGATCATTTTTACTCAGGCTGAAAATC
GCCTAGATGCACAAAAAGCGGTGATCTTAAAACTTTTAGAAAATCACCAATAG

Upstream 100 bases:

>100_bases
GGCAGAATCTGCGGCTTAAAAAAAATGGTTTTCCAAGTTCGAATCGCGGGAGATTCCTCTCGATTTCGTAACAATACAGT
CCGATACTTGTATAGAAGAT

Downstream 100 bases:

>100_bases
ACGAACATCAGGGAAATCTCAAAAAAATTCCTCGTAGAGAAAATGAAGGAGCCATTAACTCATTTTCTGCCACAGTCAAT
TTACACCTTAAGCATAATTA

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MSQVKHLISWKDWTDGEIQELLDFAVYVKKNRVYFSGHMSGRSLAMLFQKTSTRTRVSFEAGMTELGGHAIFLDWMASNF
LLSDIDFEGKYLSSNVAIIMARLKKHEDLLVLKSGSTVPVINGCCNLFHPCQSLADILTIVMDSPNDWKKKQVCYIGVHN
NVANSLVEITAALGIHLILVTPIAAKESIVAEAIDRAQKKGTVSWEMDVHKAVSHADYVYTDTWVDMEFFNDPKYQKEKE
ERIQMMMPYQVNADLMKHSKAKVMHDMPIHAGYEITREMVESDRSIIFTQAENRLDAQKAVILKLLENHQ

Sequences:

>Translated_310_residues
MSQVKHLISWKDWTDGEIQELLDFAVYVKKNRVYFSGHMSGRSLAMLFQKTSTRTRVSFEAGMTELGGHAIFLDWMASNF
LLSDIDFEGKYLSSNVAIIMARLKKHEDLLVLKSGSTVPVINGCCNLFHPCQSLADILTIVMDSPNDWKKKQVCYIGVHN
NVANSLVEITAALGIHLILVTPIAAKESIVAEAIDRAQKKGTVSWEMDVHKAVSHADYVYTDTWVDMEFFNDPKYQKEKE
ERIQMMMPYQVNADLMKHSKAKVMHDMPIHAGYEITREMVESDRSIIFTQAENRLDAQKAVILKLLENHQ
>Mature_309_residues
SQVKHLISWKDWTDGEIQELLDFAVYVKKNRVYFSGHMSGRSLAMLFQKTSTRTRVSFEAGMTELGGHAIFLDWMASNFL
LSDIDFEGKYLSSNVAIIMARLKKHEDLLVLKSGSTVPVINGCCNLFHPCQSLADILTIVMDSPNDWKKKQVCYIGVHNN
VANSLVEITAALGIHLILVTPIAAKESIVAEAIDRAQKKGTVSWEMDVHKAVSHADYVYTDTWVDMEFFNDPKYQKEKEE
RIQMMMPYQVNADLMKHSKAKVMHDMPIHAGYEITREMVESDRSIIFTQAENRLDAQKAVILKLLENHQ

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=318, Percent_Identity=32.0754716981132, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI1790703, Length=334, Percent_Identity=33.5329341317365, Blast_Score=151, Evalue=5e-38,
Organism=Escherichia coli, GI1786469, Length=332, Percent_Identity=33.1325301204819, Blast_Score=148, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6322373, Length=331, Percent_Identity=33.2326283987915, Blast_Score=148, Evalue=1e-36,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 35300; Mature: 35169

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQVKHLISWKDWTDGEIQELLDFAVYVKKNRVYFSGHMSGRSLAMLFQKTSTRTRVSFE
CCCHHHHHCCCCCCCHHHHHHHHHHHHEECCEEEEEECCCCCHHHHHHHHCCCCEEEEHH
AGMTELGGHAIFLDWMASNFLLSDIDFEGKYLSSNVAIIMARLKKHEDLLVLKSGSTVPV
CCHHHHCCCEEEEEEHHCCCEEECCCCCCCEECCCHHHHHHHHHCCCCEEEEECCCCCCH
INGCCNLFHPCQSLADILTIVMDSPNDWKKKQVCYIGVHNNVANSLVEITAALGIHLILV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHEEEEEE
TPIAAKESIVAEAIDRAQKKGTVSWEMDVHKAVSHADYVYTDTWVDMEFFNDPKYQKEKE
CCCHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHCCCEEEECCEEEEEECCCCCCHHHHH
ERIQMMMPYQVNADLMKHSKAKVMHDMPIHAGYEITREMVESDRSIIFTQAENRLDAQKA
HHHHEECCEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHH
VILKLLENHQ
HHHHHHHCCC
>Mature Secondary Structure 
SQVKHLISWKDWTDGEIQELLDFAVYVKKNRVYFSGHMSGRSLAMLFQKTSTRTRVSFE
CCHHHHHCCCCCCCHHHHHHHHHHHHEECCEEEEEECCCCCHHHHHHHHCCCCEEEEHH
AGMTELGGHAIFLDWMASNFLLSDIDFEGKYLSSNVAIIMARLKKHEDLLVLKSGSTVPV
CCHHHHCCCEEEEEEHHCCCEEECCCCCCCEECCCHHHHHHHHHCCCCEEEEECCCCCCH
INGCCNLFHPCQSLADILTIVMDSPNDWKKKQVCYIGVHNNVANSLVEITAALGIHLILV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHEEEEEE
TPIAAKESIVAEAIDRAQKKGTVSWEMDVHKAVSHADYVYTDTWVDMEFFNDPKYQKEKE
CCCHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHCCCEEEECCEEEEEECCCCCCHHHHH
ERIQMMMPYQVNADLMKHSKAKVMHDMPIHAGYEITREMVESDRSIIFTQAENRLDAQKA
HHHHEECCEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHH
VILKLLENHQ
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA