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The map label for this gene is amiA [H]

Identifier: 183221517

GI number: 183221517

Start: 2227074

End: 2227886

Strand: Reverse

Name: amiA [H]

Synonym: LEPBI_I2135

Alternate gene names: 183221517

Gene position: 2227886-2227074 (Counterclockwise)

Preceding gene: 183221518

Following gene: 183221516

Centisome position: 61.89

GC content: 44.16

Gene sequence:

>813_bases
GTGTATTTACCTCTTGATTTGGTGGAAGCTGTTTTTCTCAATTTGATTTCTTATGACGTTCGGTACCAATTTAAGGAAGG
AGAACTTTGGGTACTTTTGCCGAAGGAGACTGTCCCCAAACGAAACTTAGCTGTCAAGGCAATCATCATTGATGCGGGCC
ATGGAGGAAAAGACCCAGGAACTTCCGATCCTACAGGTTATTTTGAAAAAGAAGTGAGTCTTGGAGTGGCCCGTTATACT
TATTTGTACTTACGGAAGTACTACCCTGAAATCCGAGTGGAAATGGTCCGTAAGGATGACAGGTTTGTGGAATTGGAAGA
TCGTTCCAAATTTGCAAACCAAGTGCTCCGTGACACAAGGGATGTGATTTTTATTAGTTTCCATTGTAATGCCTCGCTCT
CAGACAAAGCCGCTGGATTCGAAGTGTATTACCTTTCCCAAAGTCCGAGTACTGAGGCTGCACGAGAGACTGCCCTTCTC
GAAAATCGTTACATCGGCAAAAACAAAAACCCAGTGGTGTCTCAGATCCAGTCCCAAATGCTTTCCAGTGTGACCCAAAG
GCGTTCGAAAAAACTGGCCGATGCGGTGGCCAATCAATACGAAAAGGGTTTGAGTCCTGAAATTCCGTCTCGTGGAGTGA
AAAAGGCAGATTTTTCCGTCTTGCGAGGAAGCCTAATGCCTGCTGTACTTGTGGAAATGGGGTATCTGACAAACCCTGAA
GAAAGTAAACGTCTCCGCGACAAATCCTTCCAAAAGAAAATTGCTCGGAGTGTGATTAAAGGAATTCATGAATACGCATC
TTCAAAAGATTAA

Upstream 100 bases:

>100_bases
TCCGCAAGGAACCATCCATTTTCGGATCGGTAGTAGTTTTTATACTTTGGATGGTAAAATTCAAAAAGTCCCCAAAGCCA
TTTTGAAGAAAGAGGAAGAG

Downstream 100 bases:

>100_bases
AGAATTGTTACAAAACTATTGGGAGTTTTTAAAAAAAATCTCCATTCAGTTTTATCGAATTGGAACCGGAGAAACCAAAC
TCACTCGTGATTTCATTTTT

Product: N-acetylmuramoyl-L-alanine amidase AmiA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MYLPLDLVEAVFLNLISYDVRYQFKEGELWVLLPKETVPKRNLAVKAIIIDAGHGGKDPGTSDPTGYFEKEVSLGVARYT
YLYLRKYYPEIRVEMVRKDDRFVELEDRSKFANQVLRDTRDVIFISFHCNASLSDKAAGFEVYYLSQSPSTEAARETALL
ENRYIGKNKNPVVSQIQSQMLSSVTQRRSKKLADAVANQYEKGLSPEIPSRGVKKADFSVLRGSLMPAVLVEMGYLTNPE
ESKRLRDKSFQKKIARSVIKGIHEYASSKD

Sequences:

>Translated_270_residues
MYLPLDLVEAVFLNLISYDVRYQFKEGELWVLLPKETVPKRNLAVKAIIIDAGHGGKDPGTSDPTGYFEKEVSLGVARYT
YLYLRKYYPEIRVEMVRKDDRFVELEDRSKFANQVLRDTRDVIFISFHCNASLSDKAAGFEVYYLSQSPSTEAARETALL
ENRYIGKNKNPVVSQIQSQMLSSVTQRRSKKLADAVANQYEKGLSPEIPSRGVKKADFSVLRGSLMPAVLVEMGYLTNPE
ESKRLRDKSFQKKIARSVIKGIHEYASSKD
>Mature_270_residues
MYLPLDLVEAVFLNLISYDVRYQFKEGELWVLLPKETVPKRNLAVKAIIIDAGHGGKDPGTSDPTGYFEKEVSLGVARYT
YLYLRKYYPEIRVEMVRKDDRFVELEDRSKFANQVLRDTRDVIFISFHCNASLSDKAAGFEVYYLSQSPSTEAARETALL
ENRYIGKNKNPVVSQIQSQMLSSVTQRRSKKLADAVANQYEKGLSPEIPSRGVKKADFSVLRGSLMPAVLVEMGYLTNPE
ESKRLRDKSFQKKIARSVIKGIHEYASSKD

Specific function: Unknown

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=231, Percent_Identity=30.3030303030303, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI87082163, Length=224, Percent_Identity=30.8035714285714, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790611, Length=229, Percent_Identity=29.2576419213974, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: 3.5.1.28

Molecular weight: Translated: 30707; Mature: 30707

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLPLDLVEAVFLNLISYDVRYQFKEGELWVLLPKETVPKRNLAVKAIIIDAGHGGKDPG
CCCCHHHHHHHHHHHHHHHHEEEEECCCEEEEECCCCCCCCCEEEEEEEEECCCCCCCCC
TSDPTGYFEKEVSLGVARYTYLYLRKYYPEIRVEMVRKDDRFVELEDRSKFANQVLRDTR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCC
DVIFISFHCNASLSDKAAGFEVYYLSQSPSTEAARETALLENRYIGKNKNPVVSQIQSQM
CEEEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LSSVTQRRSKKLADAVANQYEKGLSPEIPSRGVKKADFSVLRGSLMPAVLVEMGYLTNPE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCH
ESKRLRDKSFQKKIARSVIKGIHEYASSKD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MYLPLDLVEAVFLNLISYDVRYQFKEGELWVLLPKETVPKRNLAVKAIIIDAGHGGKDPG
CCCCHHHHHHHHHHHHHHHHEEEEECCCEEEEECCCCCCCCCEEEEEEEEECCCCCCCCC
TSDPTGYFEKEVSLGVARYTYLYLRKYYPEIRVEMVRKDDRFVELEDRSKFANQVLRDTR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCC
DVIFISFHCNASLSDKAAGFEVYYLSQSPSTEAARETALLENRYIGKNKNPVVSQIQSQM
CEEEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LSSVTQRRSKKLADAVANQYEKGLSPEIPSRGVKKADFSVLRGSLMPAVLVEMGYLTNPE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCH
ESKRLRDKSFQKKIARSVIKGIHEYASSKD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]