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The map label for this gene is exoA [H]

Identifier: 183221215

GI number: 183221215

Start: 1918120

End: 1918890

Strand: Direct

Name: exoA [H]

Synonym: LEPBI_I1829

Alternate gene names: 183221215

Gene position: 1918120-1918890 (Clockwise)

Preceding gene: 183221206

Following gene: 183221216

Centisome position: 53.29

GC content: 41.12

Gene sequence:

>771_bases
ATGAAAATCATCACGTTAAATTGCAACGGAATTCGTTCCAGTTTGAGTAAAGGTTTACTCGATTTTATACGTCACGAAAA
TCCTGACATTCTCTGTTTCCAAGAAACAAAGGCACCTGGGAAAGAAATTGACAGAGAGGAATTTCGTCACCTCGGATATG
AAGTTTATTTTTCATTGGCCGAAAAACCTGGTTACAGTGGGACCGCTGTTCTCACGAAACAAAAACCAAAACAGGTCACA
ATTGGATTTGGGGATGGGATCTTTCGTTCGGAAGGAAGGTCTGTTTTTCTCGAATTCCAAGATTTTTACCTTTGGAACCT
CTACTTTCCTTCGGGAACAAGCGGTGAAGAGCGCCAAAAGGTCAAATACCAGTTTTTAGATGAGTTTACAGAACTCACAA
AACCTTTTTTGAAAAAGAAAAAACCACTCCTGATTTGCGGAGATGTGAATATCGCCCATACAGAAATGGACATCCACAAT
CCCAAGGGGAATCAAAATAATTCCGGATTTTTACCAGCAGAACGGAAGTGGTTCACGGACTTTTTAGAACTCGGATTTTT
TGACTGCTTTCGAACCATCCATCCCGATGTTCTGGATGAATATTCCTGGTGGACCTACCGTTTCCAAGCAAGGAAAAACA
ATAAGGGTTGGAGGATTGATTATTTTTTTGTGACCAAATCCAAATCGGTTCGGCTCCAAACGGCAAAGATCGCAAAAGAA
CCAGTGATGTCAGACCATGCTCCTGTTGTTCTGGAAATCCAATTCTCTTGA

Upstream 100 bases:

>100_bases
GTGAGTTTGGACGTTCGGAGGAACGGAATTTCGGCGGAAATTTCTAACATACTGTTTTTAGTATCGACTTAAATTGGCTT
TGAATCGATCTCATTTCTAG

Downstream 100 bases:

>100_bases
CAGAATGCTTTTTTTCGGATCAATTCTTTTCTCATGAAACGATTCCTTCCTTTGCTCTCTTTTGTTCTCACCATCCAATG
TTCTGTCCTCGGTGTCCTCC

Product: exodeoxyribonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MKIITLNCNGIRSSLSKGLLDFIRHENPDILCFQETKAPGKEIDREEFRHLGYEVYFSLAEKPGYSGTAVLTKQKPKQVT
IGFGDGIFRSEGRSVFLEFQDFYLWNLYFPSGTSGEERQKVKYQFLDEFTELTKPFLKKKKPLLICGDVNIAHTEMDIHN
PKGNQNNSGFLPAERKWFTDFLELGFFDCFRTIHPDVLDEYSWWTYRFQARKNNKGWRIDYFFVTKSKSVRLQTAKIAKE
PVMSDHAPVVLEIQFS

Sequences:

>Translated_256_residues
MKIITLNCNGIRSSLSKGLLDFIRHENPDILCFQETKAPGKEIDREEFRHLGYEVYFSLAEKPGYSGTAVLTKQKPKQVT
IGFGDGIFRSEGRSVFLEFQDFYLWNLYFPSGTSGEERQKVKYQFLDEFTELTKPFLKKKKPLLICGDVNIAHTEMDIHN
PKGNQNNSGFLPAERKWFTDFLELGFFDCFRTIHPDVLDEYSWWTYRFQARKNNKGWRIDYFFVTKSKSVRLQTAKIAKE
PVMSDHAPVVLEIQFS
>Mature_256_residues
MKIITLNCNGIRSSLSKGLLDFIRHENPDILCFQETKAPGKEIDREEFRHLGYEVYFSLAEKPGYSGTAVLTKQKPKQVT
IGFGDGIFRSEGRSVFLEFQDFYLWNLYFPSGTSGEERQKVKYQFLDEFTELTKPFLKKKKPLLICGDVNIAHTEMDIHN
PKGNQNNSGFLPAERKWFTDFLELGFFDCFRTIHPDVLDEYSWWTYRFQARKNNKGWRIDYFFVTKSKSVRLQTAKIAKE
PVMSDHAPVVLEIQFS

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=258, Percent_Identity=37.5968992248062, Blast_Score=192, Evalue=3e-49,
Organism=Homo sapiens, GI18375503, Length=258, Percent_Identity=37.5968992248062, Blast_Score=192, Evalue=3e-49,
Organism=Homo sapiens, GI18375501, Length=258, Percent_Identity=37.5968992248062, Blast_Score=192, Evalue=3e-49,
Organism=Escherichia coli, GI1788046, Length=265, Percent_Identity=31.3207547169811, Blast_Score=121, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI71989536, Length=256, Percent_Identity=34.765625, Blast_Score=162, Evalue=1e-40,
Organism=Drosophila melanogaster, GI221330655, Length=254, Percent_Identity=37.7952755905512, Blast_Score=169, Evalue=1e-42,
Organism=Drosophila melanogaster, GI17136678, Length=254, Percent_Identity=37.7952755905512, Blast_Score=169, Evalue=2e-42,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29884; Mature: 29884

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIITLNCNGIRSSLSKGLLDFIRHENPDILCFQETKAPGKEIDREEFRHLGYEVYFSLA
CEEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHCCCEEEEEEC
EKPGYSGTAVLTKQKPKQVTIGFGDGIFRSEGRSVFLEFQDFYLWNLYFPSGTSGEERQK
CCCCCCCEEEEECCCCCEEEEECCCCEEECCCCEEEEEEEEEEEEEEECCCCCCCHHHHH
VKYQFLDEFTELTKPFLKKKKPLLICGDVNIAHTEMDIHNPKGNQNNSGFLPAERKWFTD
HHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEEEEEECCCCCCCCCCCCCCCHHHHHH
FLELGFFDCFRTIHPDVLDEYSWWTYRFQARKNNKGWRIDYFFVTKSKSVRLQTAKIAKE
HHHHHHHHHHHHHCHHHHHCCCEEEEEEEEECCCCCEEEEEEEEECCCCEEEEHHHHHHC
PVMSDHAPVVLEIQFS
CCCCCCCCEEEEEEEC
>Mature Secondary Structure
MKIITLNCNGIRSSLSKGLLDFIRHENPDILCFQETKAPGKEIDREEFRHLGYEVYFSLA
CEEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHCCCEEEEEEC
EKPGYSGTAVLTKQKPKQVTIGFGDGIFRSEGRSVFLEFQDFYLWNLYFPSGTSGEERQK
CCCCCCCEEEEECCCCCEEEEECCCCEEECCCCEEEEEEEEEEEEEEECCCCCCCHHHHH
VKYQFLDEFTELTKPFLKKKKPLLICGDVNIAHTEMDIHNPKGNQNNSGFLPAERKWFTD
HHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEEEEEECCCCCCCCCCCCCCCHHHHHH
FLELGFFDCFRTIHPDVLDEYSWWTYRFQARKNNKGWRIDYFFVTKSKSVRLQTAKIAKE
HHHHHHHHHHHHHCHHHHHCCCEEEEEEEEECCCCCEEEEEEEEECCCCEEEEHHHHHHC
PVMSDHAPVVLEIQFS
CCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]