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The map label for this gene is rlmL [H]

Identifier: 183221178

GI number: 183221178

Start: 1870170

End: 1871246

Strand: Direct

Name: rlmL [H]

Synonym: LEPBI_I1792

Alternate gene names: 183221178

Gene position: 1870170-1871246 (Clockwise)

Preceding gene: 183221177

Following gene: 183221179

Centisome position: 51.95

GC content: 40.02

Gene sequence:

>1077_bases
TTGGAACAAGAAATCCTTTCCTTCCATCTCAAGATAAATAATTCGAATCGAGGTGGTGTTTTCTTTTCTGGAAAAAAAGA
AGATGTCATCCAATTTGCCATTCATACTAAATTTGCCTCTCGTGTGAACCTGCAGTTGTTACACGAGAATGCCCAAAACT
ATGATGAATTTTATTCCAAAGCAATCGAACTACCATGGGAAAAATACATTGGTCCAGGTGTCAGTTTTAGCATCGATGCA
GAAACAAAAGACAAACTGAAAAACTCTGAATTTTCGATGCATCGAATGAAGGATGCCATCCTCGACAGATTACGAAGTCA
AAAAGTAGCATTACCGGAAATTCAAAAACGAATGGCGGATGTAACCATTGTTGTTAGGTCACATACGGACAGGTTCAGCA
TCGAACTTTCGTTATCGGGTGATCCGGTAGGTAGACGTGGGTATCGTTTGCATGCTGGGAATGCTCCCGTTCGGGAACCA
ATCGCTCAAGCGATGTTAGAATTATCCGAATGGAAAGAAGGAGATACTTTGCTTGATCCCATGTGTGGGTCAGGCACAAT
TCTCATTGAAGCTGCTCTCAGGGAACGGCTGTATGGCGAAATCAATCGATTCTTGTTTGCAGAATCACCTATCTTTCAAA
TTTTATTCCCTACTTATGTTTTTTCAGAAAGGAAAAAAGAAAACCCCACCACACCACATTTATTCGGATTTGACATTGAT
CCAGAAGCCATTCGCATCGCAAAAGAAAATGCGTATGAAGCCGGTGTGGAAGACTTTATCAAATTTGAAGTGGGAGATTG
TTTGGCATTAAAAAATGCATTTGGCTCAAAAGGCCATTTGGTGACCAATCCTCCCTATGGGGATCGGATTGGAAAACCGA
TGGAAGACTTACGAGAGATGTACTTTCAGTTTGGTCGGGTTTTAAAAAATGAATTTGGTGGTTGGAAGTTTACCGTCTTA
TGTGCTGATTTTTCATTATTCGGAAAATTTGGCTTAAAAGAAAACAAACATATCAGCCTCAAACATGCAAATCTAAAGGC
AAAAATAGTCGATTATGAATTACGAGGGGGGAAATGA

Upstream 100 bases:

>100_bases
TTGGATTGCCAAACTACTTGGCTCAAAAATTAGGAGATTCGGAATAAAACCTACTCACCCCACCTACCATGCCATCTGCG
GAGAGGGTCTTTCTCCGCTT

Downstream 100 bases:

>100_bases
AATCAAAGGACATTCTAAGTCGAGTTTTTGAAATCACAAGAGACCCAAGGGATGGACTTTTATTCTTAAAAGAATTCCAA
TCCCTTTCACCGGAATCATT

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MEQEILSFHLKINNSNRGGVFFSGKKEDVIQFAIHTKFASRVNLQLLHENAQNYDEFYSKAIELPWEKYIGPGVSFSIDA
ETKDKLKNSEFSMHRMKDAILDRLRSQKVALPEIQKRMADVTIVVRSHTDRFSIELSLSGDPVGRRGYRLHAGNAPVREP
IAQAMLELSEWKEGDTLLDPMCGSGTILIEAALRERLYGEINRFLFAESPIFQILFPTYVFSERKKENPTTPHLFGFDID
PEAIRIAKENAYEAGVEDFIKFEVGDCLALKNAFGSKGHLVTNPPYGDRIGKPMEDLREMYFQFGRVLKNEFGGWKFTVL
CADFSLFGKFGLKENKHISLKHANLKAKIVDYELRGGK

Sequences:

>Translated_358_residues
MEQEILSFHLKINNSNRGGVFFSGKKEDVIQFAIHTKFASRVNLQLLHENAQNYDEFYSKAIELPWEKYIGPGVSFSIDA
ETKDKLKNSEFSMHRMKDAILDRLRSQKVALPEIQKRMADVTIVVRSHTDRFSIELSLSGDPVGRRGYRLHAGNAPVREP
IAQAMLELSEWKEGDTLLDPMCGSGTILIEAALRERLYGEINRFLFAESPIFQILFPTYVFSERKKENPTTPHLFGFDID
PEAIRIAKENAYEAGVEDFIKFEVGDCLALKNAFGSKGHLVTNPPYGDRIGKPMEDLREMYFQFGRVLKNEFGGWKFTVL
CADFSLFGKFGLKENKHISLKHANLKAKIVDYELRGGK
>Mature_358_residues
MEQEILSFHLKINNSNRGGVFFSGKKEDVIQFAIHTKFASRVNLQLLHENAQNYDEFYSKAIELPWEKYIGPGVSFSIDA
ETKDKLKNSEFSMHRMKDAILDRLRSQKVALPEIQKRMADVTIVVRSHTDRFSIELSLSGDPVGRRGYRLHAGNAPVREP
IAQAMLELSEWKEGDTLLDPMCGSGTILIEAALRERLYGEINRFLFAESPIFQILFPTYVFSERKKENPTTPHLFGFDID
PEAIRIAKENAYEAGVEDFIKFEVGDCLALKNAFGSKGHLVTNPPYGDRIGKPMEDLREMYFQFGRVLKNEFGGWKFTVL
CADFSLFGKFGLKENKHISLKHANLKAKIVDYELRGGK

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=350, Percent_Identity=32.8571428571429, Blast_Score=185, Evalue=5e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 40823; Mature: 40823

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE ; PS01261 UPF0020

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQEILSFHLKINNSNRGGVFFSGKKEDVIQFAIHTKFASRVNLQLLHENAQNYDEFYSK
CCCCEEEEEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHCCCEEEHHCCCCHHHHHHH
AIELPWEKYIGPGVSFSIDAETKDKLKNSEFSMHRMKDAILDRLRSQKVALPEIQKRMAD
HHCCCHHHHCCCCCEEEECCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
VTIVVRSHTDRFSIELSLSGDPVGRRGYRLHAGNAPVREPIAQAMLELSEWKEGDTLLDP
EEEEEEECCCEEEEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCEECC
MCGSGTILIEAALRERLYGEINRFLFAESPIFQILFPTYVFSERKKENPTTPHLFGFDID
CCCCCCEEEHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCC
PEAIRIAKENAYEAGVEDFIKFEVGDCLALKNAFGSKGHLVTNPPYGDRIGKPMEDLREM
HHHHEEEHHCHHHHCHHHHHHHCCCHHHHHHHCCCCCCCEEECCCCCCCCCCCHHHHHHH
YFQFGRVLKNEFGGWKFTVLCADFSLFGKFGLKENKHISLKHANLKAKIVDYELRGGK
HHHHHHHHHHHCCCEEEEEEEECHHHHHHCCCCCCCEEEEEECCEEEEEEEEEECCCC
>Mature Secondary Structure
MEQEILSFHLKINNSNRGGVFFSGKKEDVIQFAIHTKFASRVNLQLLHENAQNYDEFYSK
CCCCEEEEEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHCCCEEEHHCCCCHHHHHHH
AIELPWEKYIGPGVSFSIDAETKDKLKNSEFSMHRMKDAILDRLRSQKVALPEIQKRMAD
HHCCCHHHHCCCCCEEEECCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
VTIVVRSHTDRFSIELSLSGDPVGRRGYRLHAGNAPVREPIAQAMLELSEWKEGDTLLDP
EEEEEEECCCEEEEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCEECC
MCGSGTILIEAALRERLYGEINRFLFAESPIFQILFPTYVFSERKKENPTTPHLFGFDID
CCCCCCEEEHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCC
PEAIRIAKENAYEAGVEDFIKFEVGDCLALKNAFGSKGHLVTNPPYGDRIGKPMEDLREM
HHHHEEEHHCHHHHCHHHHHHHCCCHHHHHHHCCCCCCCEEECCCCCCCCCCCHHHHHHH
YFQFGRVLKNEFGGWKFTVLCADFSLFGKFGLKENKHISLKHANLKAKIVDYELRGGK
HHHHHHHHHHHCCCEEEEEEEECHHHHHHCCCCCCCEEEEEECCEEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA