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The map label for this gene is rlmB [H]

Identifier: 183221112

GI number: 183221112

Start: 1798670

End: 1799371

Strand: Direct

Name: rlmB [H]

Synonym: LEPBI_I1726

Alternate gene names: 183221112

Gene position: 1798670-1799371 (Clockwise)

Preceding gene: 183221111

Following gene: 183221113

Centisome position: 49.97

GC content: 38.89

Gene sequence:

>702_bases
ATGGCTCCAGAACGGGGTATCAAAACCATCCGGGAAGTCATTGTTAAGGATGGAATTGGAAATGAAGAAAAACAAAGGAT
CAAAGCCTATCTTCCTAATTCCGTAAAGTTTACAACAGTTTCTGCAAGGGAATTGGATCGGATTGCCCAAGATAAAAACC
ACCAAGGTTATGTGATCATTCGAACGAAACAAAAATCTTATTTATCTCTTGGATTTGAACAATTCAAACAAAATATAAAA
CCGAACGAAGGTCCTGTTTTGATCCTAGACAGAATCCAAGATCCTGGAAATTTAGGGAATTTATTACGAACAGCTGAGTG
TATGGGCGTTAAACATGTGTTAATGTCTGATAGGGACACTTCCCCTATCACTCCCGTTGTGGAAAAAGTTTCAGCAGGTG
CCATTCACCATATTCAAATCTACCGAGTCGCCAATTTGAAACATGGAATCGATTTTTTAAAGAAAAATGAATATTGGATT
CTAGCCACTGACGAAGAAGGTGAAGAAGGGATATGGGAAACGTTACCAGATGTATCACAAATGGCTGTGATCATGGGAAA
TGAAGGAGAAGGAGTCAAAAGAATTTTACTTGAAGATGCTGATTATGTAGCTAGAATTCCATTGTATGGCGCTGTTACAT
CCTTAAACGTGGTGGTAGCTTGCGGAATTACTTTGGATAGGCTTCATCATGCTTCCTTATAG

Upstream 100 bases:

>100_bases
TCACAGACACAAAAGAAGGAAATACAACATGGAAAAAAGCCCAGTAGAAATACTCTTTGGTAAACGAAATTTTTTCGAAT
TTTTAGAATCATTGGAACAA

Downstream 100 bases:

>100_bases
CAGAATCCGAATTGTTTTAGTATTCCTTGTATTATTTCTATTCACTTCCGAAGCCTGTATTTATGATTTTTATCGAAAGG
AATTTGTTTCGGAAGATCAA

Product: TrmH family tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 233; Mature: 232

Protein sequence:

>233_residues
MAPERGIKTIREVIVKDGIGNEEKQRIKAYLPNSVKFTTVSARELDRIAQDKNHQGYVIIRTKQKSYLSLGFEQFKQNIK
PNEGPVLILDRIQDPGNLGNLLRTAECMGVKHVLMSDRDTSPITPVVEKVSAGAIHHIQIYRVANLKHGIDFLKKNEYWI
LATDEEGEEGIWETLPDVSQMAVIMGNEGEGVKRILLEDADYVARIPLYGAVTSLNVVVACGITLDRLHHASL

Sequences:

>Translated_233_residues
MAPERGIKTIREVIVKDGIGNEEKQRIKAYLPNSVKFTTVSARELDRIAQDKNHQGYVIIRTKQKSYLSLGFEQFKQNIK
PNEGPVLILDRIQDPGNLGNLLRTAECMGVKHVLMSDRDTSPITPVVEKVSAGAIHHIQIYRVANLKHGIDFLKKNEYWI
LATDEEGEEGIWETLPDVSQMAVIMGNEGEGVKRILLEDADYVARIPLYGAVTSLNVVVACGITLDRLHHASL
>Mature_232_residues
APERGIKTIREVIVKDGIGNEEKQRIKAYLPNSVKFTTVSARELDRIAQDKNHQGYVIIRTKQKSYLSLGFEQFKQNIKP
NEGPVLILDRIQDPGNLGNLLRTAECMGVKHVLMSDRDTSPITPVVEKVSAGAIHHIQIYRVANLKHGIDFLKKNEYWIL
ATDEEGEEGIWETLPDVSQMAVIMGNEGEGVKRILLEDADYVARIPLYGAVTSLNVVVACGITLDRLHHASL

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=159, Percent_Identity=33.3333333333333, Blast_Score=73, Evalue=2e-13,
Organism=Escherichia coli, GI1790623, Length=233, Percent_Identity=32.1888412017167, Blast_Score=103, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17555908, Length=246, Percent_Identity=29.6747967479675, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324775, Length=168, Percent_Identity=31.547619047619, Blast_Score=79, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26052; Mature: 25921

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPERGIKTIREVIVKDGIGNEEKQRIKAYLPNSVKFTTVSARELDRIAQDKNHQGYVII
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEECHHHHHHHHHCCCCCCEEEE
RTKQKSYLSLGFEQFKQNIKPNEGPVLILDRIQDPGNLGNLLRTAECMGVKHVLMSDRDT
EECCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
SPITPVVEKVSAGAIHHIQIYRVANLKHGIDFLKKNEYWILATDEEGEEGIWETLPDVSQ
CCCHHHHHHHCCCCEEEEEEEEECCCHHHHHHHCCCCEEEEEECCCCCCCHHHHCCCHHH
MAVIMGNEGEGVKRILLEDADYVARIPLYGAVTSLNVVVACGITLDRLHHASL
EEEEECCCCCCHHHHHHCCCCCEEECCHHHHHHHCEEEEECCCHHHHHHHCCC
>Mature Secondary Structure 
APERGIKTIREVIVKDGIGNEEKQRIKAYLPNSVKFTTVSARELDRIAQDKNHQGYVII
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEECHHHHHHHHHCCCCCCEEEE
RTKQKSYLSLGFEQFKQNIKPNEGPVLILDRIQDPGNLGNLLRTAECMGVKHVLMSDRDT
EECCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
SPITPVVEKVSAGAIHHIQIYRVANLKHGIDFLKKNEYWILATDEEGEEGIWETLPDVSQ
CCCHHHHHHHCCCCEEEEEEEEECCCHHHHHHHCCCCEEEEEECCCCCCCHHHHCCCHHH
MAVIMGNEGEGVKRILLEDADYVARIPLYGAVTSLNVVVACGITLDRLHHASL
EEEEECCCCCCHHHHHHCCCCCEEECCHHHHHHHCEEEEECCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12704232 [H]