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The map label for this gene is yiaO [H]

Identifier: 183220863

GI number: 183220863

Start: 1535634

End: 1536641

Strand: Reverse

Name: yiaO [H]

Synonym: LEPBI_I1476

Alternate gene names: 183220863

Gene position: 1536641-1535634 (Counterclockwise)

Preceding gene: 183220864

Following gene: 183220862

Centisome position: 42.69

GC content: 42.16

Gene sequence:

>1008_bases
ATGTTTTTAAAGCAGTTAAAATATTTAGTTTGTGTAAGTATCGCCCTCACGATCAGTGGGGGTCTTTTTGCTCAAACAAC
CGTTAAACTCGCTACCGTTGCTCCCGAAGGATCACCTTGGGCCAACGAATTAGCAAAAATCAAAAAGAAAATTGAAGCTG
AATCCCAAGGACAAATTAAATTCAAAATTTATCCTGGTGGACAGATGGGTGGAGAAAACGAAATCCTCCAACAAGTCATT
CGTGGGAAACTACAAGGTGCTGGACTCACTGCCGGAGCACTTGCCAACACTGTCAAAGAATTAAATGTTTTGGAAATTCC
TTACCTTTTTAATTCTTATGCACAAGCAGATTGTGTTCTCGACGAACATCTATTAGAAGATTTTAGAAAACTTTTTGAAG
CGAAGGGTCTTATTTTTGTCACTTGGGCAGAAAACGGGTACCGTTCCATCGGAACCAAATCAGCTCCCGTGAAAAAACCT
GAAGACTTAAAGGGAATCAAAATCAGGGTGCAAGAATCTCCAGTTCATATTGCCTATTGGAAACAATTAGGTGTGAGTGG
AATTCCCATTGCGATCCCAGAAGTCCTTCCCTCCTTACAAACAGGTGTGGTGGAAGGGTTTGACAACACTCCCCTTTTCA
CTCTTGCAGCGGAATGGCAAACTGCGATCAAATACTTCACTTTGACCCGCCATATTTACCAACCAGCAGCCATCCTATAC
TCTAAAAAGTATTGGGATACTCTCAATGATGAACAAAAGAAAACCCTCATGGGTGAAGGAAACAAACTTGCTCCAGGTGC
TAGACAAGCAGTTCGTTCCATCGAAAAGAATATGATTGCCACTCTAAAAAAAGCAGATGTGCAAGTGTATGAGCCTTCAG
CGGCAGACCTTGCTGGATTTAAATCTGCTGCAGGTGTTGTAGCAGGCCAAGTGATTGGAAAAATTGGTGGTCAATCGAAA
CAGATCTACGATAAAATCCAAAAAGCCAAAGCGGCTTGTGGCCCATAG

Upstream 100 bases:

>100_bases
AACCATCTTCGTTACCTGAAATTGAAGCTGACCAAATCGTTGAACAACGAAAAGCGAAAAAATTACTCGACGAATTATAA
ACCATCACTAGGAGAACTAG

Downstream 100 bases:

>100_bases
AGTAAGGAAGGAAACAGATAAATGAAATTCGTCGAACGAATTCTAAATACATTGAGTTTCGGCGAGAAATGGGCAGGGGG
AATTTGTTTCCTTCTGCTCA

Product: ABC transporter periplasmic binding protein

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MFLKQLKYLVCVSIALTISGGLFAQTTVKLATVAPEGSPWANELAKIKKKIEAESQGQIKFKIYPGGQMGGENEILQQVI
RGKLQGAGLTAGALANTVKELNVLEIPYLFNSYAQADCVLDEHLLEDFRKLFEAKGLIFVTWAENGYRSIGTKSAPVKKP
EDLKGIKIRVQESPVHIAYWKQLGVSGIPIAIPEVLPSLQTGVVEGFDNTPLFTLAAEWQTAIKYFTLTRHIYQPAAILY
SKKYWDTLNDEQKKTLMGEGNKLAPGARQAVRSIEKNMIATLKKADVQVYEPSAADLAGFKSAAGVVAGQVIGKIGGQSK
QIYDKIQKAKAACGP

Sequences:

>Translated_335_residues
MFLKQLKYLVCVSIALTISGGLFAQTTVKLATVAPEGSPWANELAKIKKKIEAESQGQIKFKIYPGGQMGGENEILQQVI
RGKLQGAGLTAGALANTVKELNVLEIPYLFNSYAQADCVLDEHLLEDFRKLFEAKGLIFVTWAENGYRSIGTKSAPVKKP
EDLKGIKIRVQESPVHIAYWKQLGVSGIPIAIPEVLPSLQTGVVEGFDNTPLFTLAAEWQTAIKYFTLTRHIYQPAAILY
SKKYWDTLNDEQKKTLMGEGNKLAPGARQAVRSIEKNMIATLKKADVQVYEPSAADLAGFKSAAGVVAGQVIGKIGGQSK
QIYDKIQKAKAACGP
>Mature_335_residues
MFLKQLKYLVCVSIALTISGGLFAQTTVKLATVAPEGSPWANELAKIKKKIEAESQGQIKFKIYPGGQMGGENEILQQVI
RGKLQGAGLTAGALANTVKELNVLEIPYLFNSYAQADCVLDEHLLEDFRKLFEAKGLIFVTWAENGYRSIGTKSAPVKKP
EDLKGIKIRVQESPVHIAYWKQLGVSGIPIAIPEVLPSLQTGVVEGFDNTPLFTLAAEWQTAIKYFTLTRHIYQPAAILY
SKKYWDTLNDEQKKTLMGEGNKLAPGARQAVRSIEKNMIATLKKADVQVYEPSAADLAGFKSAAGVVAGQVIGKIGGQSK
QIYDKIQKAKAACGP

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=260, Percent_Identity=28.4615384615385, Blast_Score=129, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 36485; Mature: 36485

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLKQLKYLVCVSIALTISGGLFAQTTVKLATVAPEGSPWANELAKIKKKIEAESQGQIK
CCHHHHHHHHHHHHHHHHCCCEEEHHEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEE
FKIYPGGQMGGENEILQQVIRGKLQGAGLTAGALANTVKELNVLEIPYLFNSYAQADCVL
EEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCEEECCHHHHHHHHHHHHH
DEHLLEDFRKLFEAKGLIFVTWAENGYRSIGTKSAPVKKPEDLKGIKIRVQESPVHIAYW
HHHHHHHHHHHHHHCCEEEEEECCCCCHHCCCCCCCCCCCCCCCCEEEEEECCCEEEEEH
KQLGVSGIPIAIPEVLPSLQTGVVEGFDNTPLFTLAAEWQTAIKYFTLTRHIYQPAAILY
HHCCCCCCCEEHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCHHHHH
SKKYWDTLNDEQKKTLMGEGNKLAPGARQAVRSIEKNMIATLKKADVQVYEPSAADLAGF
HHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHH
KSAAGVVAGQVIGKIGGQSKQIYDKIQKAKAACGP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFLKQLKYLVCVSIALTISGGLFAQTTVKLATVAPEGSPWANELAKIKKKIEAESQGQIK
CCHHHHHHHHHHHHHHHHCCCEEEHHEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEE
FKIYPGGQMGGENEILQQVIRGKLQGAGLTAGALANTVKELNVLEIPYLFNSYAQADCVL
EEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCEEECCHHHHHHHHHHHHH
DEHLLEDFRKLFEAKGLIFVTWAENGYRSIGTKSAPVKKPEDLKGIKIRVQESPVHIAYW
HHHHHHHHHHHHHHCCEEEEEECCCCCHHCCCCCCCCCCCCCCCCEEEEEECCCEEEEEH
KQLGVSGIPIAIPEVLPSLQTGVVEGFDNTPLFTLAAEWQTAIKYFTLTRHIYQPAAILY
HHCCCCCCCEEHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCHHHHH
SKKYWDTLNDEQKKTLMGEGNKLAPGARQAVRSIEKNMIATLKKADVQVYEPSAADLAGF
HHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHH
KSAAGVVAGQVIGKIGGQSKQIYDKIQKAKAACGP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]