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The map label for this gene is pcnB [H]

Identifier: 183220775

GI number: 183220775

Start: 1442567

End: 1444060

Strand: Direct

Name: pcnB [H]

Synonym: LEPBI_I1386

Alternate gene names: 183220775

Gene position: 1442567-1444060 (Clockwise)

Preceding gene: 183220773

Following gene: 183220777

Centisome position: 40.07

GC content: 40.16

Gene sequence:

>1494_bases
GTGGATGAATCCAATATGTTTAAATTTCTCACTTCTCTTTTCAGAAAGAAAGCCGACTCTGTCGATTCCTTTTTGATGTA
CCCCGAGGGAAAGAGATACTATCGAGAAACTCACTCCATACGCAGGGCCAATATCGATGAAGATGCCATCAAAATCATCA
ATCGATTGAACAAGTTTCGTTACAAGGCCTATTTAGTCGGTGGAGGAGTCAGAGATCTGCTGATGGGCAAACGCCCAAAA
GATTTTGACATTGTCACAAGTGCGACACCAAACCAAATCAAAAGGGTCTTCAATAACTGCCGAATCATCGGTAAACGATT
TAAAATTGTACACATCATTTTTAAAGGTAAAATTATTGAAGTCTCCACGTTCCGATCATTACCGGAACATCGTTTGGAAA
AACACAAAGCAGAAAACGATTATCTCATCAAGCGGGACAACTCCTTCGGTACAGCAAAGGAAGACGCGGCAAGACGCGAC
TTTACCATCAATTCACTGTTCTACGATCCTAAAAACGATTCCATTTTGGATTACGTTGGCGGATTTGAAGACATCCAAAA
GAAAATCGTAAGGGTCATTGGTGATCCTGATATTTCCTTTAAAGAAGATCCAGTTCGTATGTTACGAGCGGTTAAGTTTT
CCGTTTTACTCGGTCTCGACATCGAGAAAAAAACCAAACTTGCCATCAAAAAGAACCGACTGGAACTCGAAAAGTCTTCT
ACTGCGAGACTTCTTGAAGAATACAACAAGATGTTTCGTACATGGAAAACTTCCATCATCTTTGAAGGTTTGGCGGAAAA
CCATCTCCTTGATGTATTGTACAAAGAACCAACTGATAAGTTAAAGAAAACGGATCCGGAATGGCGTGAACATTTTATGG
ACACTCCACTTGGGAAAAGACTCGCTGTGACTGACAAACTCCTCTCGGCCAGAGAAGAGATGACTCCTGCGATATTTTAT
TCGTTAATCTTTTACGATATCGTCAAAGATCTTTATGAAAATGATCGTGGCCACTTAGCGCATAACATCAAAGAAAGTTT
ACAACCAGTTTTCGAACGTATGGGTATCCCCAAACGTGAACAAGATAACTTGGTGAAAATTTTTATCAGCCAACCTCGTT
TTCAAGTCACTGACGATGAAAAAGAAAGGCAAAATACTTTCTTCAAAAAGAAAGATTATTTTTACGATGCGTTTATGGTG
TATAAAATTGTCGCCATTTCCGAAAACAATGAAGCCGCCGTACAAAGTGCCTTCTTTTGGGAAATTTCACTCAGACAAAG
ACCAAAACCGGACAGCCATCAGTTTGGCCAACAGAATCGTAAAAAAGAAGGGAATAAAAAACGCCCACCAAGGAAAAAAC
ACAGGGATCGAAGGGGTGGAAATCCAAACCAAAGAGACCAAGATTCCCAAGGGAACCATTCGAACCAATCTGGAAAAGAG
GATTCAAATGAAGGTCGTAATTCTTCATTCGAACACCAAGATTCGGAAGAATAA

Upstream 100 bases:

>100_bases
CGCAATTTGGAGCCTTTCGTAGGCGGTTGTGACGTAAGTTTCTGCCAATGTTCGTTATCCAGCCAATTTTTTCCTTGCCC
ATGCCATTTTTCAGGGGAAA

Downstream 100 bases:

>100_bases
AAACCTGTCGATTCGTATCCTGTAGAATCTCTCCATAACTCTCGTCAAATGATCTTGGGAGTTATGGAAGGATCACCCAA
TATAAGATTTTAATTGTCGA

Product: Poly(A) polymerase

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MDESNMFKFLTSLFRKKADSVDSFLMYPEGKRYYRETHSIRRANIDEDAIKIINRLNKFRYKAYLVGGGVRDLLMGKRPK
DFDIVTSATPNQIKRVFNNCRIIGKRFKIVHIIFKGKIIEVSTFRSLPEHRLEKHKAENDYLIKRDNSFGTAKEDAARRD
FTINSLFYDPKNDSILDYVGGFEDIQKKIVRVIGDPDISFKEDPVRMLRAVKFSVLLGLDIEKKTKLAIKKNRLELEKSS
TARLLEEYNKMFRTWKTSIIFEGLAENHLLDVLYKEPTDKLKKTDPEWREHFMDTPLGKRLAVTDKLLSAREEMTPAIFY
SLIFYDIVKDLYENDRGHLAHNIKESLQPVFERMGIPKREQDNLVKIFISQPRFQVTDDEKERQNTFFKKKDYFYDAFMV
YKIVAISENNEAAVQSAFFWEISLRQRPKPDSHQFGQQNRKKEGNKKRPPRKKHRDRRGGNPNQRDQDSQGNHSNQSGKE
DSNEGRNSSFEHQDSEE

Sequences:

>Translated_497_residues
MDESNMFKFLTSLFRKKADSVDSFLMYPEGKRYYRETHSIRRANIDEDAIKIINRLNKFRYKAYLVGGGVRDLLMGKRPK
DFDIVTSATPNQIKRVFNNCRIIGKRFKIVHIIFKGKIIEVSTFRSLPEHRLEKHKAENDYLIKRDNSFGTAKEDAARRD
FTINSLFYDPKNDSILDYVGGFEDIQKKIVRVIGDPDISFKEDPVRMLRAVKFSVLLGLDIEKKTKLAIKKNRLELEKSS
TARLLEEYNKMFRTWKTSIIFEGLAENHLLDVLYKEPTDKLKKTDPEWREHFMDTPLGKRLAVTDKLLSAREEMTPAIFY
SLIFYDIVKDLYENDRGHLAHNIKESLQPVFERMGIPKREQDNLVKIFISQPRFQVTDDEKERQNTFFKKKDYFYDAFMV
YKIVAISENNEAAVQSAFFWEISLRQRPKPDSHQFGQQNRKKEGNKKRPPRKKHRDRRGGNPNQRDQDSQGNHSNQSGKE
DSNEGRNSSFEHQDSEE
>Mature_497_residues
MDESNMFKFLTSLFRKKADSVDSFLMYPEGKRYYRETHSIRRANIDEDAIKIINRLNKFRYKAYLVGGGVRDLLMGKRPK
DFDIVTSATPNQIKRVFNNCRIIGKRFKIVHIIFKGKIIEVSTFRSLPEHRLEKHKAENDYLIKRDNSFGTAKEDAARRD
FTINSLFYDPKNDSILDYVGGFEDIQKKIVRVIGDPDISFKEDPVRMLRAVKFSVLLGLDIEKKTKLAIKKNRLELEKSS
TARLLEEYNKMFRTWKTSIIFEGLAENHLLDVLYKEPTDKLKKTDPEWREHFMDTPLGKRLAVTDKLLSAREEMTPAIFY
SLIFYDIVKDLYENDRGHLAHNIKESLQPVFERMGIPKREQDNLVKIFISQPRFQVTDDEKERQNTFFKKKDYFYDAFMV
YKIVAISENNEAAVQSAFFWEISLRQRPKPDSHQFGQQNRKKEGNKKRPPRKKHRDRRGGNPNQRDQDSQGNHSNQSGKE
DSNEGRNSSFEHQDSEE

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=218, Percent_Identity=31.1926605504587, Blast_Score=81, Evalue=3e-15,
Organism=Escherichia coli, GI87081691, Length=321, Percent_Identity=38.3177570093458, Blast_Score=235, Evalue=4e-63,
Organism=Caenorhabditis elegans, GI71995920, Length=210, Percent_Identity=31.4285714285714, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI281360112, Length=209, Percent_Identity=33.9712918660287, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21357337, Length=209, Percent_Identity=33.9712918660287, Blast_Score=93, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 58507; Mature: 58507

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDESNMFKFLTSLFRKKADSVDSFLMYPEGKRYYRETHSIRRANIDEDAIKIINRLNKFR
CCCHHHHHHHHHHHHHHHCCHHHEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
YKAYLVGGGVRDLLMGKRPKDFDIVTSATPNQIKRVFNNCRIIGKRFKIVHIIFKGKIIE
EEEEEECCCHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCEEEEEEEECCEEEE
VSTFRSLPEHRLEKHKAENDYLIKRDNSFGTAKEDAARRDFTINSLFYDPKNDSILDYVG
EHHHHCCHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHCCEEEEEEEECCCCCCHHHHHC
GFEDIQKKIVRVIGDPDISFKEDPVRMLRAVKFSVLLGLDIEKKTKLAIKKNRLELEKSS
CHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
TARLLEEYNKMFRTWKTSIIFEGLAENHLLDVLYKEPTDKLKKTDPEWREHFMDTPLGKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHCCCCCCH
LAVTDKLLSAREEMTPAIFYSLIFYDIVKDLYENDRGHLAHNIKESLQPVFERMGIPKRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCC
QDNLVKIFISQPRFQVTDDEKERQNTFFKKKDYFYDAFMVYKIVAISENNEAAVQSAFFW
CCCEEEEEECCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHEE
EISLRQRPKPDSHQFGQQNRKKEGNKKRPPRKKHRDRRGGNPNQRDQDSQGNHSNQSGKE
EEECCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
DSNEGRNSSFEHQDSEE
CCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDESNMFKFLTSLFRKKADSVDSFLMYPEGKRYYRETHSIRRANIDEDAIKIINRLNKFR
CCCHHHHHHHHHHHHHHHCCHHHEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
YKAYLVGGGVRDLLMGKRPKDFDIVTSATPNQIKRVFNNCRIIGKRFKIVHIIFKGKIIE
EEEEEECCCHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCEEEEEEEECCEEEE
VSTFRSLPEHRLEKHKAENDYLIKRDNSFGTAKEDAARRDFTINSLFYDPKNDSILDYVG
EHHHHCCHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHCCEEEEEEEECCCCCCHHHHHC
GFEDIQKKIVRVIGDPDISFKEDPVRMLRAVKFSVLLGLDIEKKTKLAIKKNRLELEKSS
CHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
TARLLEEYNKMFRTWKTSIIFEGLAENHLLDVLYKEPTDKLKKTDPEWREHFMDTPLGKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHCCCCCCH
LAVTDKLLSAREEMTPAIFYSLIFYDIVKDLYENDRGHLAHNIKESLQPVFERMGIPKRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCC
QDNLVKIFISQPRFQVTDDEKERQNTFFKKKDYFYDAFMVYKIVAISENNEAAVQSAFFW
CCCEEEEEECCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHEE
EISLRQRPKPDSHQFGQQNRKKEGNKKRPPRKKHRDRRGGNPNQRDQDSQGNHSNQSGKE
EEECCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
DSNEGRNSSFEHQDSEE
CCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]