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The map label for this gene is glpK

Identifier: 183220713

GI number: 183220713

Start: 1378369

End: 1379865

Strand: Direct

Name: glpK

Synonym: LEPBI_I1323

Alternate gene names: 183220713

Gene position: 1378369-1379865 (Clockwise)

Preceding gene: 183220712

Following gene: 183220720

Centisome position: 38.29

GC content: 41.82

Gene sequence:

>1497_bases
ATGGCAAAAAAAAATTACATCATTGGAATTGATGCAGGTACAACTGGGATTCGTACATTTTGTTTTAATGACAAAGGGAA
AGTGATCTCCTCGGCTTACCAAGAGTTCAAACAATACTATCCAAAACCAGGTTGGGTGGAACATGACCCAGAAGAGATTT
GGGTAAAAACACAAAAGCTCATCACACTTGCCATTAAAAATGGAAAGTTAAATCCCAAAGATGCCGTGGCCATTGGGATC
ACAAACCAAAGGGAAACTTCGGTTGTTTGGGATAAAAAAACAGGAAAACCTGTTTATAATGCCATCGTTTGGCAATGCCG
ACGTACTTCTGATATTTGTAAGGACTTAAAAAAACAAAGCCTAGATTCCAATTTTCGAAATAAAACAGGGCTAGTCCTAG
ATGCTTATTTTTCAGGTACCAAAATCCAATGGATCTTAGACAATGTGAAAGGGGCAAGGGATCGGGCAGAACGTGGTGAC
CTTCTTTTTGGAACCATTGATACCTGGTTATTGTACAAACTCACAGGCCATAAAGAACATAAAACCGATCATACCAATGC
ATCTCGAACCTTACTTTTTAATATCCAAACCAAAGAATGGGATGAAGAACTATGTAAGATTTTAAGAGTTCCCATGTCCA
TGTTACCGAAGGCATTTAACTCCAAAAATCTCTTTGGGTTTACTTCTAATGTCAAATCCATCCCAGATGGAATCCCAATT
TCTTCGTTAGTTGGTGACCAACAAGGGGCACTCTTTGGACAACTTTGTACAGAACCAGGGGAAGCCAAAAATACCTATGG
AACGGGTTGTTTTTTACTATTCAATGTGGGGGATGAATTCCGGATTTCGAACCAAGGTCTCATCACCACATTGGCCCTCG
GTCCAGAAGGGAAAACTGTGTACTGTTTGGAAGGATCTGTTTTTATCGGGGGTGCTGTGGTCCAATTCCTCCGAGACAAT
TTGGAATTTTTTAAATACTCAAAAGACTCGGAAAAACTCGTGAAGTCGATCAAAACAAAAGATGATATTGTGTTTGTTCC
TGCCTTTGCAGGTCTTGGTGCCCCGCATTGGGACCAAGAAGCACGTGGTGCCATCTTTGGACTCTCTCGTGACACAACGC
CTGCCCAAATCACAAGAGCCGCTCTCAAAGCCATTGCCTTACAATCCTATGAACTTGCCAATGCAATGGAAAAGGAAACG
GGAAAACCATTGAAATTTTTACGAGTGGATGGTGGTGCCACTTCCAACGCATGGCTCATGCAATTCCAAGCTGACATCTT
AGGAACAAAAGTGATCCGACCACAAAACGTGGATACGACGGTGCTGGGTGCGGCATACTTGGCAGGACTCGAACGTGGAT
TTTTTAAATCAGTGGCTCACCTTCGAAAAGAAGAAACCAAAACCACACAATTCACACCGAAGATGAAAGAATCCGAACGG
AAAGAAGAAATTGATAAGTGGAATTTAGCCATTTCTCGTGTGAAAACGGAAACCTAA

Upstream 100 bases:

>100_bases
TTGGTATACTTACGAGCGTTAAAGTACAAAGAGATGTTTGATCCTCATGGAAAATTAGAAATCATTATGGCGTATGAAGG
TTTGGAAATAGAGGTATAAT

Downstream 100 bases:

>100_bases
TCCATTCCCTTCAATAGTCGAACCATAGGAGAGACGATTCAAAGGTTTGTTTTTGTTTGGTAATCCCTCCACCAACAAGA
AACAAACCAGTTTCATAAAA

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MAKKNYIIGIDAGTTGIRTFCFNDKGKVISSAYQEFKQYYPKPGWVEHDPEEIWVKTQKLITLAIKNGKLNPKDAVAIGI
TNQRETSVVWDKKTGKPVYNAIVWQCRRTSDICKDLKKQSLDSNFRNKTGLVLDAYFSGTKIQWILDNVKGARDRAERGD
LLFGTIDTWLLYKLTGHKEHKTDHTNASRTLLFNIQTKEWDEELCKILRVPMSMLPKAFNSKNLFGFTSNVKSIPDGIPI
SSLVGDQQGALFGQLCTEPGEAKNTYGTGCFLLFNVGDEFRISNQGLITTLALGPEGKTVYCLEGSVFIGGAVVQFLRDN
LEFFKYSKDSEKLVKSIKTKDDIVFVPAFAGLGAPHWDQEARGAIFGLSRDTTPAQITRAALKAIALQSYELANAMEKET
GKPLKFLRVDGGATSNAWLMQFQADILGTKVIRPQNVDTTVLGAAYLAGLERGFFKSVAHLRKEETKTTQFTPKMKESER
KEEIDKWNLAISRVKTET

Sequences:

>Translated_498_residues
MAKKNYIIGIDAGTTGIRTFCFNDKGKVISSAYQEFKQYYPKPGWVEHDPEEIWVKTQKLITLAIKNGKLNPKDAVAIGI
TNQRETSVVWDKKTGKPVYNAIVWQCRRTSDICKDLKKQSLDSNFRNKTGLVLDAYFSGTKIQWILDNVKGARDRAERGD
LLFGTIDTWLLYKLTGHKEHKTDHTNASRTLLFNIQTKEWDEELCKILRVPMSMLPKAFNSKNLFGFTSNVKSIPDGIPI
SSLVGDQQGALFGQLCTEPGEAKNTYGTGCFLLFNVGDEFRISNQGLITTLALGPEGKTVYCLEGSVFIGGAVVQFLRDN
LEFFKYSKDSEKLVKSIKTKDDIVFVPAFAGLGAPHWDQEARGAIFGLSRDTTPAQITRAALKAIALQSYELANAMEKET
GKPLKFLRVDGGATSNAWLMQFQADILGTKVIRPQNVDTTVLGAAYLAGLERGFFKSVAHLRKEETKTTQFTPKMKESER
KEEIDKWNLAISRVKTET
>Mature_497_residues
AKKNYIIGIDAGTTGIRTFCFNDKGKVISSAYQEFKQYYPKPGWVEHDPEEIWVKTQKLITLAIKNGKLNPKDAVAIGIT
NQRETSVVWDKKTGKPVYNAIVWQCRRTSDICKDLKKQSLDSNFRNKTGLVLDAYFSGTKIQWILDNVKGARDRAERGDL
LFGTIDTWLLYKLTGHKEHKTDHTNASRTLLFNIQTKEWDEELCKILRVPMSMLPKAFNSKNLFGFTSNVKSIPDGIPIS
SLVGDQQGALFGQLCTEPGEAKNTYGTGCFLLFNVGDEFRISNQGLITTLALGPEGKTVYCLEGSVFIGGAVVQFLRDNL
EFFKYSKDSEKLVKSIKTKDDIVFVPAFAGLGAPHWDQEARGAIFGLSRDTTPAQITRAALKAIALQSYELANAMEKETG
KPLKFLRVDGGATSNAWLMQFQADILGTKVIRPQNVDTTVLGAAYLAGLERGFFKSVAHLRKEETKTTQFTPKMKESERK
EEIDKWNLAISRVKTET

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI42794763, Length=459, Percent_Identity=46.1873638344227, Blast_Score=393, Evalue=1e-109,
Organism=Homo sapiens, GI4504007, Length=455, Percent_Identity=46.3736263736264, Blast_Score=390, Evalue=1e-108,
Organism=Homo sapiens, GI189409120, Length=455, Percent_Identity=46.3736263736264, Blast_Score=390, Evalue=1e-108,
Organism=Homo sapiens, GI41393575, Length=439, Percent_Identity=46.9248291571754, Blast_Score=389, Evalue=1e-108,
Organism=Homo sapiens, GI88196792, Length=506, Percent_Identity=32.4110671936759, Blast_Score=288, Evalue=8e-78,
Organism=Escherichia coli, GI1790361, Length=493, Percent_Identity=49.4929006085193, Blast_Score=530, Evalue=1e-151,
Organism=Escherichia coli, GI1789987, Length=471, Percent_Identity=23.5668789808917, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790005, Length=484, Percent_Identity=22.5206611570248, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17535599, Length=498, Percent_Identity=38.7550200803213, Blast_Score=354, Evalue=7e-98,
Organism=Caenorhabditis elegans, GI71986140, Length=499, Percent_Identity=26.8537074148297, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI71986147, Length=258, Percent_Identity=30.6201550387597, Blast_Score=105, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6321755, Length=549, Percent_Identity=35.5191256830601, Blast_Score=298, Evalue=1e-81,
Organism=Drosophila melanogaster, GI17864214, Length=511, Percent_Identity=40.7045009784736, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655555, Length=494, Percent_Identity=39.4736842105263, Blast_Score=355, Evalue=3e-98,
Organism=Drosophila melanogaster, GI24655560, Length=494, Percent_Identity=39.4736842105263, Blast_Score=355, Evalue=3e-98,
Organism=Drosophila melanogaster, GI24655570, Length=494, Percent_Identity=39.4736842105263, Blast_Score=355, Evalue=3e-98,
Organism=Drosophila melanogaster, GI24655574, Length=494, Percent_Identity=39.4736842105263, Blast_Score=355, Evalue=3e-98,
Organism=Drosophila melanogaster, GI24655565, Length=494, Percent_Identity=39.4736842105263, Blast_Score=355, Evalue=3e-98,
Organism=Drosophila melanogaster, GI24654573, Length=438, Percent_Identity=42.2374429223744, Blast_Score=348, Evalue=6e-96,
Organism=Drosophila melanogaster, GI19922058, Length=533, Percent_Identity=35.2720450281426, Blast_Score=308, Evalue=7e-84,
Organism=Drosophila melanogaster, GI24652871, Length=491, Percent_Identity=35.8452138492872, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI24656367, Length=510, Percent_Identity=30.7843137254902, Blast_Score=245, Evalue=4e-65,
Organism=Drosophila melanogaster, GI21357867, Length=525, Percent_Identity=30.0952380952381, Blast_Score=239, Evalue=3e-63,
Organism=Drosophila melanogaster, GI24656372, Length=345, Percent_Identity=31.8840579710145, Blast_Score=175, Evalue=6e-44,
Organism=Drosophila melanogaster, GI45552909, Length=285, Percent_Identity=29.8245614035088, Blast_Score=121, Evalue=1e-27,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_LEPBA (B0SG67)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001962369.1
- ProteinModelPortal:   B0SG67
- SMR:   B0SG67
- GeneID:   6387377
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_1269
- HOGENOM:   HBG511469
- OMA:   KPSSEVY
- ProtClustDB:   CLSK574221
- BioCyc:   LBIF355278:LBF_1269-MONOMER
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 55700; Mature: 55569

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 14-14 BINDING 18-18 BINDING 84-84 BINDING 136-136 BINDING 246-246 BINDING 268-268 BINDING 311-311

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKNYIIGIDAGTTGIRTFCFNDKGKVISSAYQEFKQYYPKPGWVEHDPEEIWVKTQKL
CCCCCEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHEEE
ITLAIKNGKLNPKDAVAIGITNQRETSVVWDKKTGKPVYNAIVWQCRRTSDICKDLKKQS
EEEEEECCCCCCCCEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHH
LDSNFRNKTGLVLDAYFSGTKIQWILDNVKGARDRAERGDLLFGTIDTWLLYKLTGHKEH
HCCCCCCCCCEEEEEEECCCEEEEEECCCCCCHHHHHCCCEEEEEHHHEEEEEECCCCCC
KTDHTNASRTLLFNIQTKEWDEELCKILRVPMSMLPKAFNSKNLFGFTSNVKSIPDGIPI
CCCCCCCCEEEEEEEECCHHHHHHHHHHHCCHHHHHHHCCCCCEEEECCCCCCCCCCCCH
SSLVGDQQGALFGQLCTEPGEAKNTYGTGCFLLFNVGDEFRISNQGLITTLALGPEGKTV
HHHHCCCCCCHHHHHHCCCCCCCCCCCCEEEEEEECCCCEEECCCCEEEEEEECCCCCEE
YCLEGSVFIGGAVVQFLRDNLEFFKYSKDSEKLVKSIKTKDDIVFVPAFAGLGAPHWDQE
EEECCCEEEHHHHHHHHHHCHHHEECCCCHHHHHHHHCCCCCEEEEECHHCCCCCCCCCC
ARGAIFGLSRDTTPAQITRAALKAIALQSYELANAMEKETGKPLKFLRVDGGATSNAWLM
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEE
QFQADILGTKVIRPQNVDTTVLGAAYLAGLERGFFKSVAHLRKEETKTTQFTPKMKESER
EEEHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
KEEIDKWNLAISRVKTET
HHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AKKNYIIGIDAGTTGIRTFCFNDKGKVISSAYQEFKQYYPKPGWVEHDPEEIWVKTQKL
CCCCEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHEEE
ITLAIKNGKLNPKDAVAIGITNQRETSVVWDKKTGKPVYNAIVWQCRRTSDICKDLKKQS
EEEEEECCCCCCCCEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHH
LDSNFRNKTGLVLDAYFSGTKIQWILDNVKGARDRAERGDLLFGTIDTWLLYKLTGHKEH
HCCCCCCCCCEEEEEEECCCEEEEEECCCCCCHHHHHCCCEEEEEHHHEEEEEECCCCCC
KTDHTNASRTLLFNIQTKEWDEELCKILRVPMSMLPKAFNSKNLFGFTSNVKSIPDGIPI
CCCCCCCCEEEEEEEECCHHHHHHHHHHHCCHHHHHHHCCCCCEEEECCCCCCCCCCCCH
SSLVGDQQGALFGQLCTEPGEAKNTYGTGCFLLFNVGDEFRISNQGLITTLALGPEGKTV
HHHHCCCCCCHHHHHHCCCCCCCCCCCCEEEEEEECCCCEEECCCCEEEEEEECCCCCEE
YCLEGSVFIGGAVVQFLRDNLEFFKYSKDSEKLVKSIKTKDDIVFVPAFAGLGAPHWDQE
EEECCCEEEHHHHHHHHHHCHHHEECCCCHHHHHHHHCCCCCEEEEECHHCCCCCCCCCC
ARGAIFGLSRDTTPAQITRAALKAIALQSYELANAMEKETGKPLKFLRVDGGATSNAWLM
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEE
QFQADILGTKVIRPQNVDTTVLGAAYLAGLERGFFKSVAHLRKEETKTTQFTPKMKESER
EEEHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
KEEIDKWNLAISRVKTET
HHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA