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The map label for this gene is nuoN

Identifier: 183220697

GI number: 183220697

Start: 1361442

End: 1362884

Strand: Direct

Name: nuoN

Synonym: LEPBI_I1307

Alternate gene names: 183220697

Gene position: 1361442-1362884 (Clockwise)

Preceding gene: 183220696

Following gene: 183220698

Centisome position: 37.82

GC content: 41.37

Gene sequence:

>1443_bases
ATGTCATATACTCCTTCCTCGAATGACTTAATAGCGATTTCACCCATGCTCATATTGTGTGGGGTAGCTTTACTTTCCCT
AGTTGTCCAATTTTTAATCCCAGAAGAAGATGAAGGGAAACCACTTTGGGTTCTTTCTATTTTAGGAATCCTTGTGGCTA
TGTATGCTTTGTATCATACAACCAATTCACCAGGATATGGAAAGTTTTTTGGTTCTCAAATTTCGATTAGCCCACTGACG
GTTTGGTTAAGTGCTATCTATTTAATAGCAGGTCTCATCACCTTACTCGTTGCACCTCCCTTTTTATCACAACACAAAAC
ATTATTCCCTGAGTTTTTCCCCTTAATGCTCTTTTGTTTGTCTGGTATGATGTTTTTGACATCTGGGTATGACCTCATTG
TCATCTTTGTAGGATTGGAAATCCTTTCCTTATCACTTTATGTGATGATAGGTATGGCTCGGACATCTGTTTCCGCATTG
GAAAGTGCGATGAAGTATTTTTTACTCGGAACATTTAGTTCTGGTTTTATGTTACTAGGGATTGCATTTTTATACGGCGG
ATCGGGGACAACGAACTTGGACGGAGCCCTTCGAGGATTATCCTTAAAAGGATATGAGGCGAATTTTTCGAAACTAGGTC
TTGGATTGTTTTTTGTGGGTGTGTCCTTTAAAGCTGCCCTTGTTCCATTCCACTCCTGGACACCCGATGTGTATGAAGGA
GCACAAACTCCTATCACTGGTTTTATGGCGAGTGCGGGAAAGGCATCCGCCCTTGGACTTGTTATCATTTTGTTCAACCA
TATCCCAATGGGTGAGATGGGAAATGTTTGGAAGTATCTTATGGGAACCATTGCCCTGATTTCTATGACTTGGGGGAATA
TCGTTGCTTTAAAACAAGATAACCTAAAACGGATGTTAGCCTATTCTTCGATTTCCCATGCCGGTTATATTGTAGCAGGG
ATTGCTTGTGGGGCAGGTCTTGAAGCACTGTATTATCTTTTTTCTTATTCCTTACTGAACCTTGCTGCCTTTGCCATCAT
TTCCTATTTGGAACAAGGAAAACATGAGGTGACTGTGAATGGAATCTCTCACTTAAGTGGTGAACATCCATTCACAGCTC
TTGCTTTGAGTCTCGTGTTTTTGTCCTTTGCTGGGTTTCCACCCCTCATCGGTTTTTGGACCAAACTTTTCCTTTTGCAA
AAAATGGCGGAATCGGATTTATTCTTCCACCGGGTTTTGTTATTTGGAGCTGTTGCCAATTCTTGTATCGCATTTTATTA
TTATATGAAGATCACCATCCAGTCCTATATGAAACAAGAGACTGGGGTTGTGGCCGGGGCACGGGATCTGCCGAGTTTGC
CAACACTCGGATTTTTAATCTTTTTGCTCTGTGTTTTCTTTACGGCAGGTTGGATTTTTTTCCAGCCAGGATCCCTTTTG
TAA

Upstream 100 bases:

>100_bases
TCTTCCCAAAGTGGAAGAGGATTTAGAGAATTTAGAAAATTCCATCGAGTCAGATTTTGATTTGGAATTAGAACCGAACG
AGAAAAAAGGAAACTAACAA

Downstream 100 bases:

>100_bases
TTCTGTTATAGCAGAATTACATCCAGGATAGCGAAAAACAAGTTGCTTCCCAATCCCAGGGGAAAAGGCTTGCCTAAGTA
GGTAAATGGAAATGGCAACG

Product: NADH-quinone oxidoreductase subunit N

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MSYTPSSNDLIAISPMLILCGVALLSLVVQFLIPEEDEGKPLWVLSILGILVAMYALYHTTNSPGYGKFFGSQISISPLT
VWLSAIYLIAGLITLLVAPPFLSQHKTLFPEFFPLMLFCLSGMMFLTSGYDLIVIFVGLEILSLSLYVMIGMARTSVSAL
ESAMKYFLLGTFSSGFMLLGIAFLYGGSGTTNLDGALRGLSLKGYEANFSKLGLGLFFVGVSFKAALVPFHSWTPDVYEG
AQTPITGFMASAGKASALGLVIILFNHIPMGEMGNVWKYLMGTIALISMTWGNIVALKQDNLKRMLAYSSISHAGYIVAG
IACGAGLEALYYLFSYSLLNLAAFAIISYLEQGKHEVTVNGISHLSGEHPFTALALSLVFLSFAGFPPLIGFWTKLFLLQ
KMAESDLFFHRVLLFGAVANSCIAFYYYMKITIQSYMKQETGVVAGARDLPSLPTLGFLIFLLCVFFTAGWIFFQPGSLL

Sequences:

>Translated_480_residues
MSYTPSSNDLIAISPMLILCGVALLSLVVQFLIPEEDEGKPLWVLSILGILVAMYALYHTTNSPGYGKFFGSQISISPLT
VWLSAIYLIAGLITLLVAPPFLSQHKTLFPEFFPLMLFCLSGMMFLTSGYDLIVIFVGLEILSLSLYVMIGMARTSVSAL
ESAMKYFLLGTFSSGFMLLGIAFLYGGSGTTNLDGALRGLSLKGYEANFSKLGLGLFFVGVSFKAALVPFHSWTPDVYEG
AQTPITGFMASAGKASALGLVIILFNHIPMGEMGNVWKYLMGTIALISMTWGNIVALKQDNLKRMLAYSSISHAGYIVAG
IACGAGLEALYYLFSYSLLNLAAFAIISYLEQGKHEVTVNGISHLSGEHPFTALALSLVFLSFAGFPPLIGFWTKLFLLQ
KMAESDLFFHRVLLFGAVANSCIAFYYYMKITIQSYMKQETGVVAGARDLPSLPTLGFLIFLLCVFFTAGWIFFQPGSLL
>Mature_479_residues
SYTPSSNDLIAISPMLILCGVALLSLVVQFLIPEEDEGKPLWVLSILGILVAMYALYHTTNSPGYGKFFGSQISISPLTV
WLSAIYLIAGLITLLVAPPFLSQHKTLFPEFFPLMLFCLSGMMFLTSGYDLIVIFVGLEILSLSLYVMIGMARTSVSALE
SAMKYFLLGTFSSGFMLLGIAFLYGGSGTTNLDGALRGLSLKGYEANFSKLGLGLFFVGVSFKAALVPFHSWTPDVYEGA
QTPITGFMASAGKASALGLVIILFNHIPMGEMGNVWKYLMGTIALISMTWGNIVALKQDNLKRMLAYSSISHAGYIVAGI
ACGAGLEALYYLFSYSLLNLAAFAIISYLEQGKHEVTVNGISHLSGEHPFTALALSLVFLSFAGFPPLIGFWTKLFLLQK
MAESDLFFHRVLLFGAVANSCIAFYYYMKITIQSYMKQETGVVAGARDLPSLPTLGFLIFLLCVFFTAGWIFFQPGSLL

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family

Homologues:

Organism=Escherichia coli, GI145693160, Length=468, Percent_Identity=31.8376068376068, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1788827, Length=317, Percent_Identity=27.1293375394322, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1788831, Length=291, Percent_Identity=26.1168384879725, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788614, Length=230, Percent_Identity=24.3478260869565, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NUON_LEPBA (B0SFU5)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001962353.1
- GeneID:   6389039
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_1253
- HOGENOM:   HBG747830
- OMA:   MDMNTLL
- ProtClustDB:   CLSK573684
- BioCyc:   LBIF355278:LBF_1253-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_00445
- InterPro:   IPR010096
- InterPro:   IPR001750
- TIGRFAMs:   TIGR01770

Pfam domain/function: PF00361 Oxidored_q1

EC number: =1.6.99.5

Molecular weight: Translated: 52222; Mature: 52090

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1ace7bc4)-; HASH(0x1ae3df30)-; HASH(0x1ad86858)-; HASH(0x1ae3b5fc)-; HASH(0x1ab5202c)-; HASH(0x1ae3b608)-; HASH(0x1ae3b740)-; HASH(0x1ae3b77c)-; HASH(0x1acd98a8)-; HASH(0x1ae3b8cc)-; HASH(0x1ad86840)-; HASH(0x1ae16a1c)-; HASH(0x1ad81468)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYTPSSNDLIAISPMLILCGVALLSLVVQFLIPEEDEGKPLWVLSILGILVAMYALYHT
CCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHC
TNSPGYGKFFGSQISISPLTVWLSAIYLIAGLITLLVAPPFLSQHKTLFPEFFPLMLFCL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
SGMMFLTSGYDLIVIFVGLEILSLSLYVMIGMARTSVSALESAMKYFLLGTFSSGFMLLG
CCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
IAFLYGGSGTTNLDGALRGLSLKGYEANFSKLGLGLFFVGVSFKAALVPFHSWTPDVYEG
HHHHHCCCCCCCCCHHHHCCEECCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCHHHCC
AQTPITGFMASAGKASALGLVIILFNHIPMGEMGNVWKYLMGTIALISMTWGNIVALKQD
CCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHH
NLKRMLAYSSISHAGYIVAGIACGAGLEALYYLFSYSLLNLAAFAIISYLEQGKHEVTVN
HHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEC
GISHLSGEHPFTALALSLVFLSFAGFPPLIGFWTKLFLLQKMAESDLFFHRVLLFGAVAN
CHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SCIAFYYYMKITIQSYMKQETGVVAGARDLPSLPTLGFLIFLLCVFFTAGWIFFQPGSLL
HHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCH
>Mature Secondary Structure 
SYTPSSNDLIAISPMLILCGVALLSLVVQFLIPEEDEGKPLWVLSILGILVAMYALYHT
CCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHC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PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA