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The map label for this gene is nuoF [H]

Identifier: 183220691

GI number: 183220691

Start: 1354494

End: 1355765

Strand: Direct

Name: nuoF [H]

Synonym: LEPBI_I1301

Alternate gene names: 183220691

Gene position: 1354494-1355765 (Clockwise)

Preceding gene: 183220690

Following gene: 183220692

Centisome position: 37.63

GC content: 43.87

Gene sequence:

>1272_bases
ATGGGACTAAAAACCTTACTTACAACTCATATTGATGCTTCTGATTCGCATACTTTGAAACACTACCAGTCGGTCGGCGG
ATATGAAAGTCTAAAAAAGGCACTTTCTGAAATGACCGCCGAACAGATTGTAAACGATGTAAAGAACTCAGGACTCCGTG
GTCGCGGTGGTGCTGGTTTTCCTACGGGAAACAAATGGGGATTCATTCCCAAAACAGACAAACCGAAATACTTAATTTGT
AATGGCGATGAAGGTGAACCTGGAACCTTTAAAGATAGGATGCTCATTGAACGTTTCCCTCATATGCTCATTGAAGGGAT
GGCCATTGCCGCAAAAGCAATCGATTCTCACCAAGGGTATATCTACATCCGAGGTGAATTTCACAAGGGCATTCGCATTG
TAGAAACTGCTGTGGAAGAAGCATACAAAGCAGGCCTACTTGGAAAAAATATCTTTGGCCTAGGTTATGATTTTGATTTA
GCTGTGTATTCAGGAGCAGGTGCTTATATCTGTGGAGAAGAGTCAGCTCTTATCAATTCACTCGAAGGCCGGAGGGGCCA
CCCACGTTTGAAACCTCCGTTTCCAGCAGTTTCAGGGCTTTATGCGTGCCCAACGGTTGTGAACAATGTCGAAACATTTT
GTAATGTGCCACATATCATCCGTATGACGGGCGAGGAATACAAAAAAATTGGAACCGAAAAATCTCCTGGCACTCGTCTC
TTTGCGGTGAGTGGTCACGTCAAAAAACCAGGGATTTATGAAGTGGAAATGGGTACTCCGATGAAGGAACTCATTTTCGA
TATTTGTGGTGGGATCAAAAACGATAAGGATCTCAAGGCTGTGATCCCAGGGGGAAGCTCTTCTCCCATCCTAACAAAAG
ACGAAGCGATGACTGCCACGATGGATTATGAATCCATTGCTTCTCTCAAATCGATGTTAGGATCTGGGGCAGTGATCATT
TTGTCGGAAGAAGCAGACCTTGTGGAAACCACATACCGATTGGCAGAATTTTATTCACATGAATCTTGTGGCCAATGTAC
ACCATGCCGCGAAGGAACCCATTGGGTCAAAGATCTACTTCATAAAATCAAAAAGGGAGAAGGGACTGAAAAAGATGTGG
AACTCATCTTCTCTCTTTCAAGGAATATGGAAGGTGGCACCACCATTTGTCCGTTAGCGGATGCGTGTGTGATGGCAGTT
CGACCAACCATGACAAAATTTAAGGAAGAGTTTTCTCTTCGATTGAAAAAGGAAGTGAGTGTTTCTCACTAA

Upstream 100 bases:

>100_bases
GGATTTGGTCCTGTTGCCCAAATCAATGACAAATATTATGAAAATTTAACTCCAGAATCCATCGAAAAGATTCTTTCTGA
ATTGGAAAAAGAAGGATAAC

Downstream 100 bases:

>100_bases
AGGTCACCAAATACGATGGACTGGGCTTTAATTCTTGCTTGGGGGATCAAAATCCTCTCATTATTTTTTGTAATCCTTAC
TGGTGTTGCGTATTATACCC

Product: NADH-quinone oxidoreductase subunit F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MGLKTLLTTHIDASDSHTLKHYQSVGGYESLKKALSEMTAEQIVNDVKNSGLRGRGGAGFPTGNKWGFIPKTDKPKYLIC
NGDEGEPGTFKDRMLIERFPHMLIEGMAIAAKAIDSHQGYIYIRGEFHKGIRIVETAVEEAYKAGLLGKNIFGLGYDFDL
AVYSGAGAYICGEESALINSLEGRRGHPRLKPPFPAVSGLYACPTVVNNVETFCNVPHIIRMTGEEYKKIGTEKSPGTRL
FAVSGHVKKPGIYEVEMGTPMKELIFDICGGIKNDKDLKAVIPGGSSSPILTKDEAMTATMDYESIASLKSMLGSGAVII
LSEEADLVETTYRLAEFYSHESCGQCTPCREGTHWVKDLLHKIKKGEGTEKDVELIFSLSRNMEGGTTICPLADACVMAV
RPTMTKFKEEFSLRLKKEVSVSH

Sequences:

>Translated_423_residues
MGLKTLLTTHIDASDSHTLKHYQSVGGYESLKKALSEMTAEQIVNDVKNSGLRGRGGAGFPTGNKWGFIPKTDKPKYLIC
NGDEGEPGTFKDRMLIERFPHMLIEGMAIAAKAIDSHQGYIYIRGEFHKGIRIVETAVEEAYKAGLLGKNIFGLGYDFDL
AVYSGAGAYICGEESALINSLEGRRGHPRLKPPFPAVSGLYACPTVVNNVETFCNVPHIIRMTGEEYKKIGTEKSPGTRL
FAVSGHVKKPGIYEVEMGTPMKELIFDICGGIKNDKDLKAVIPGGSSSPILTKDEAMTATMDYESIASLKSMLGSGAVII
LSEEADLVETTYRLAEFYSHESCGQCTPCREGTHWVKDLLHKIKKGEGTEKDVELIFSLSRNMEGGTTICPLADACVMAV
RPTMTKFKEEFSLRLKKEVSVSH
>Mature_422_residues
GLKTLLTTHIDASDSHTLKHYQSVGGYESLKKALSEMTAEQIVNDVKNSGLRGRGGAGFPTGNKWGFIPKTDKPKYLICN
GDEGEPGTFKDRMLIERFPHMLIEGMAIAAKAIDSHQGYIYIRGEFHKGIRIVETAVEEAYKAGLLGKNIFGLGYDFDLA
VYSGAGAYICGEESALINSLEGRRGHPRLKPPFPAVSGLYACPTVVNNVETFCNVPHIIRMTGEEYKKIGTEKSPGTRLF
AVSGHVKKPGIYEVEMGTPMKELIFDICGGIKNDKDLKAVIPGGSSSPILTKDEAMTATMDYESIASLKSMLGSGAVIIL
SEEADLVETTYRLAEFYSHESCGQCTPCREGTHWVKDLLHKIKKGEGTEKDVELIFSLSRNMEGGTTICPLADACVMAVR
PTMTKFKEEFSLRLKKEVSVSH

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=412, Percent_Identity=45.3883495145631, Blast_Score=376, Evalue=1e-104,
Organism=Homo sapiens, GI260656005, Length=412, Percent_Identity=45.3883495145631, Blast_Score=375, Evalue=1e-104,
Organism=Escherichia coli, GI1788620, Length=414, Percent_Identity=45.6521739130435, Blast_Score=387, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17531727, Length=412, Percent_Identity=45.873786407767, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI19920794, Length=380, Percent_Identity=47.6315789473684, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI45550452, Length=381, Percent_Identity=48.0314960629921, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24653819, Length=387, Percent_Identity=37.2093023255814, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24653817, Length=357, Percent_Identity=35.2941176470588, Blast_Score=233, Evalue=2e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 46263; Mature: 46132

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS00644 COMPLEX1_51K_1 ; PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLKTLLTTHIDASDSHTLKHYQSVGGYESLKKALSEMTAEQIVNDVKNSGLRGRGGAGF
CCCHHHHHEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PTGNKWGFIPKTDKPKYLICNGDEGEPGTFKDRMLIERFPHMLIEGMAIAAKAIDSHQGY
CCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
IYIRGEFHKGIRIVETAVEEAYKAGLLGKNIFGLGYDFDLAVYSGAGAYICGEESALINS
EEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCEEEEEECCCCEEEECCHHHHHHH
LEGRRGHPRLKPPFPAVSGLYACPTVVNNVETFCNVPHIIRMTGEEYKKIGTEKSPGTRL
HCCCCCCCCCCCCCCHHHCHHHHHHHHHHHHHHHCCCHHEEECCHHHHHCCCCCCCCCEE
FAVSGHVKKPGIYEVEMGTPMKELIFDICGGIKNDKDLKAVIPGGSSSPILTKDEAMTAT
EEEECCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCCEEECCCCEEEE
MDYESIASLKSMLGSGAVIILSEEADLVETTYRLAEFYSHESCGQCTPCREGTHWVKDLL
CCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
HKIKKGEGTEKDVELIFSLSRNMEGGTTICPLADACVMAVRPTMTKFKEEFSLRLKKEVS
HHHHCCCCCHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VSH
CCC
>Mature Secondary Structure 
GLKTLLTTHIDASDSHTLKHYQSVGGYESLKKALSEMTAEQIVNDVKNSGLRGRGGAGF
CCHHHHHEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PTGNKWGFIPKTDKPKYLICNGDEGEPGTFKDRMLIERFPHMLIEGMAIAAKAIDSHQGY
CCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
IYIRGEFHKGIRIVETAVEEAYKAGLLGKNIFGLGYDFDLAVYSGAGAYICGEESALINS
EEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCEEEEEECCCCEEEECCHHHHHHH
LEGRRGHPRLKPPFPAVSGLYACPTVVNNVETFCNVPHIIRMTGEEYKKIGTEKSPGTRL
HCCCCCCCCCCCCCCHHHCHHHHHHHHHHHHHHHCCCHHEEECCHHHHHCCCCCCCCCEE
FAVSGHVKKPGIYEVEMGTPMKELIFDICGGIKNDKDLKAVIPGGSSSPILTKDEAMTAT
EEEECCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCCEEECCCCEEEE
MDYESIASLKSMLGSGAVIILSEEADLVETTYRLAEFYSHESCGQCTPCREGTHWVKDLL
CCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
HKIKKGEGTEKDVELIFSLSRNMEGGTTICPLADACVMAVRPTMTKFKEEFSLRLKKEVS
HHHHCCCCCHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VSH
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]